Nature Methods - An integrated open-source acquisition and analysis ecosystem for high-throughput single-molecule imaging

This study explores the use of graphene reservoirs formed over perforated supporting films as a method to precisely control ice thickness during cryo-EM sample preparation.

Translational perturbations trigger ubiquitination and turnover of 40S ribosomes, yet how these events are coupled remains unclear. Shah et al. reveal that RIOK3 remodels ubiquitinated 40S subunits, e...

RNase P RNA forms shifting active and inactive conformers whose catalytic activity depends on Mg2+-driven tertiary-interaction dynamics, which are modulated by binding of accessory proteins.

Ribosome malfunction has toxic consequences, but how mammalian cells select ribosomal subunits for degradation remains poorly understood. Here, we develop a pulse-chase CRISPR screening strategy to id...

The Integrated Stress Response is a critical eukaryotic signaling pathway that maintains cellular proteostasis. During amino acid scarcity, the yeast kinase Gcn2 (GCN2 in humans) and its activators Gc...

Dynamic ribosome heterogeneity has emerged as a potential layer of translational regulation, but whether variation in ribosomal RNA (rRNA) modification stoichiometry can selectively control the transl...

In the absence of canonical, promoter-based transcriptional regulation, Leishmania has evolved alternative regulatory mechanisms for adaptive gene expression, including post-transcriptional control vi...

A recent study reveals how a single stop codon helps alphaviruses evade immune defenses in both mosquitoes and humans

Tsai et al. discover that ribosome composition is reprogrammed in response to intrinsic and external cellular perturbations, which supports ribosome remodeling as a conserved mechanism that enables ad...

Ribosome hibernation helps cells survive stress by reversibly silencing translation and preserving ribosomal complexes. Although well characterized in bacteria and eukaryotes, archaeal hibernation rem...

A working catalogue of twelve open problems in biology — each a concrete, testable target for the field.

Paper2Agent converts research papers into interactive artificial intelligence agents by turning manuscripts, code and data into model context protocol-based tool-invoking systems that reproduce origin...

Prime assembly enables RNA-guided integration of medium-to-large DNA sequences in human cells without requiring double-strand breaks or cell cycle progression, and supports exon recoding, transgene in...

The ribosomal rRNA methyltransferase GidB is involved in the discrimination of mischarged tRNAs in mycobacteria.

When used as antibacterial agents, Peptide Nucleic Acid (PNAs) are generally designed to base-pair with complementary sequences of an essential mRNA and block translation initiation. Although bacteria...

Here, we describe the determination of the highest resolution structure in the PDB at 0.43 Å resolution. Electron-density maps reveal extensive deformation electron density that is completely accounte...

Bacterial ribosomes can synthesize proteins encoded within their own RNA.

The slow growth displayed by Mycobacterium tuberculosis has been historically associated with pathogen’s persistence and drug tolerance. Unexpectedly, here the authors identify a rare subpopulation of...

Taurine is an abundant sulfur-containing metabolite with diverse roles in cellular physiology across many organisms, yet its biosynthesis and biological functions remain largely unexplored in fungi. H...

Candida auris colonizes metabolically diverse host niches and modulates immunity to its advantage. This study shows that carbon source availability regulates C. auris adhesion and cell morphology, the...

All living beings use ribosomes to synthesize proteins from α-amino acids. Inherited from our common ancestor, a few universally conserved ribosome biogenesis steps, introducing essential ribosomal RN...

Isoaspartate (isoAsp) formation is typically viewed as a "molecular clock" through nonenzymatic degradation of aspartate or asparagine during protein aging. Here we report a nearly universal enzymatic...

Bacterial ribosomal RNAs (rRNAs) are decorated with conserved nucleotide modifications, but the functionality of these modifications is often underexplored. MraW (RsmH) is a 16S rRNA methyltransferase. Here, we report that deletion of mraW corrects a late-stage sporulation defect in Bacillus subtili …

Abstract. To successfully transfer, conjugative plasmids must overcome a recipient cell’s defence mechanisms. The leading region of many plasmids—the first

Abstract. Ribosomal RNA (rRNA) modifications cluster around the peptidyl transferase centre (PTC), the catalytic centre of the ribosome, yet their collecti

The locations and levels of RNA modifications are key to understanding their biological functions. Here, the authors develop proteomics-style, RNA-specific isobaric tandem mass tags for quantitative…

In this work, Kalanxhi and Laxminarayan review the evidence supporting the links between climate change and antimicrobial resistance, highlight opportunities for action and identify areas wherein furt...

The loss of FMRP does not affect how neuronal ribosomes are stalled or where they are stalled, but reduces the number of neuronal RNA granules that contain stalled ribosomes.

Biochemistry publishes exceptional rigorous high-impact research across all of biological chemistry.

Cell growth requires elevated protein synthesis, which depends on the production of ribosomes. Ribosome biogenesis is a complex, multi-step pathway in which newly transcribed precursor ribosomal RNA (...

Mitochondria perform diverse metabolic and signaling functions, yet how these activities are spatially organized within the mitochondrial network of cells remains poorly understood. Organelle contact ...