40S ribosomes are degraded in diverse stress conditions. Diehl et al. find that in response to stress, TUT7 is recruited to ubiquitylated 40S ribosomes and adds 3′ oligo(U) tails to the 18S rRNA. Iter...

Translational perturbations trigger ubiquitination and turnover of 40S ribosomes, yet how these events are coupled remains unclear. Shah et al. reveal that RIOK3 remodels ubiquitinated 40S subunits, e...

The Integrated Stress Response is a critical eukaryotic signaling pathway that maintains cellular proteostasis. During amino acid scarcity, the yeast kinase Gcn2 (GCN2 in humans) and its activators Gc...

Ribosome malfunction has toxic consequences, but how mammalian cells select ribosomal subunits for degradation remains poorly understood. Here, we develop a pulse-chase CRISPR screening strategy to id...

RNase P RNA forms shifting active and inactive conformers whose catalytic activity depends on Mg2+-driven tertiary-interaction dynamics, which are modulated by binding of accessory proteins.

Tsai et al. discover that ribosome composition is reprogrammed in response to intrinsic and external cellular perturbations, which supports ribosome remodeling as a conserved mechanism that enables ad...

A recent study reveals how a single stop codon helps alphaviruses evade immune defenses in both mosquitoes and humans

Abstract. The type VI secretion system (T6SS) is a sophisticated machine that uses a contractile mechanism to deliver antibacterial toxins into neighboring

Ribosome hibernation helps cells survive stress by reversibly silencing translation and preserving ribosomal complexes. Although well characterized in bacteria and eukaryotes, archaeal hibernation rem...

Drug development requires evidence integration across biological scales and modalities, but relevant tools are fragmented. We introduce the Virtual Biotech, an organization of artificial intelligence ...

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Cryo-electron tomography (cryo-ET) resolves and quantifies an almost complete view of bacterial translation, revealing how gene expression machineries coordinate across the processes of transcription,...

In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly p...

Fluorescent proteins are widely used as quantitative reporters of protein abundance in living cells. Here we show that this relationship can break down under translation inhibition: the cellular ribos...

Millions of RNA sequences are readily available, but the structures that determine their function are not. Picornaviruses initiate translation through Internal Ribosome Entry Sites (IRESes), RNA eleme...

Abstract. Sparsomycin (SPA) is a broad-spectrum inhibitor of protein synthesis with activity across all three domains of life. Although SPA has long been k

In this Perspective, Levin and Wang give a brief history of bacterial growth rate and discuss how it is connected to nutrient availability and macromolecule synthesis, with a focus on (p)ppGpp as a ce...

A working catalogue of twelve open problems in biology — each a concrete, testable target for the field.

Paper2Agent converts research papers into interactive artificial intelligence agents by turning manuscripts, code and data into model context protocol-based tool-invoking systems that reproduce origin...

The ciliary transition zone (TZ) regulates ciliary proteome composition, yet its molecular architecture, protein content, and contribution to motile ciliopathies remain poorly defined. We applied in situ cryo-electron tomography and subtomogram averaging ...