iiinova — where your digital self keeps evolving

Pablo J. Sáez @pjsaez.bsky.social · 11/10/2026

Tomorrow, new MSc students start their program I'll make them play the classic "spot the differences"👇🏽, bc The North remembers...& The Mad Microscopy Gang #TMMG too I'll try to follow @cilialab.bsky.social & @aaandmoore.bsky.social to keep my chill & not start ranting #microscopy #AI #AIslop🧪🔬

The picture shows a parallel of the Nikon small work competition webpage showing the removal of the 1st place, which inappropriately used AI in the movie of beating cilia.

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Pablo J. Sáez @pjsaez.bsky.social · 30/09/2026

Two cakes...two "last pieces"... I guess only @isabel-stoetzel.bsky.social will understand, bc I don't 😅🤷🏽‍♂️

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Pablo J. Sáez @pjsaez.bsky.social · 18/09/2026

#Mechanobiology rules! Today, it's not only Chile's independence day🇨🇱😋 ...but also Sara Wickström @sarawickstrom.bsky.social is getting the Körber Prize 👏🏽👏🏽👏🏽 for being a "curiosity's junkie" 😂 Of note, Kate photobombing is top @katemiro.bsky.social @focalplane.bsky.social @koerber-ip.bsky.social

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Pablo J. Sáez @pjsaez.bsky.social · 15/09/2026

Relieved to remember that after 1 M versions + last minute changes somehow, somewhere there is light @ the end of the tunnel... Grateful for the work of my @cellcommlab.bsky.social Whenever is ready we'll post it to receive comments from the community 😉 Tks 4 existing @biorxivpreprint.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 02/09/2026

Another year is gone & I grow old and proud of the work of my team @cellcommlab.bsky.social To celebrate the 6th labversary, we did the best we can do: we submitted...now to cross fingers & to press F5. OK, to celebrate we also had pizza as tradition dictates ;) #CellMigration & pizza lovers 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 18/08/2026

#cytoskeleton So, there is a small (very small) 9-aa motif that is an evolutionary conserved anchor to actin. It's named: short linear actin filament-binding motif (SFM), and it's *the same* found in LifeAct ! Full: www.nature.com/articles/s41... @natcellbio.nature.com @cellclub.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 11/06/2026

We kicked off today the 1st session of the Focal Plane #CellMigration webinars Brilliant talks by @juanmagararc.bsky.social , @kyohalie.bsky.social & @djcohen.bsky.social Tks to Helen @focalplane.bsky.social for organization & support. Looking FWD to the next one @valeriaventurini.bsky.social !

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Pablo J. Sáez @pjsaez.bsky.social · 04/06/2026

1 week to start🔥 New @focalplane.bsky.social webinar series focused on #CellMigration, register & join us▶️, it will be fun! pls spread the word, in the next one, ECRs could apply to show their work ;) ▶️ to register: focalplane.biologists.com/2026/05/29/n... @dgz.bsky.social @the-node.bsky.social

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Pablo J. Sáez @pjsaez.bsky.social · 27/04/2026

Very nice cover picture for the next GRC in #CellMigration, chaired by @robinsall.bsky.social #AnaMariaLennon & @djcohen.bsky.social www.grc.org/directed-cel...

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Pablo J. Sáez @pjsaez.bsky.social · 09/04/2026

#FijiImageJ macro time! It's been a while...but somehow it feels like riding a bike. #microscopy analysis #bioimaging #organelles

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Pablo J. Sáez @pjsaez.bsky.social · 30/03/2026

Just because it's Monday, we love #microscopy & we love #mitochondria, the powerhouse of the #cell We videoed mito under the microscope to see their dynamics. Here they join forces! Ofc we <3 the other #organelles too (to prevent jealousy) From @cellcommlab.bsky.social 🧪🔬 @focalplane.bsky.social

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Pablo J. Sáez @pjsaez.bsky.social · 16/03/2026

This is why we love #CalciumSignaling Look how mechanical damage triggers long range Ca2+ waves in this plant !! By @annalisabellandi.bsky.social, who is now around here ;) Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 16/01/2026

#FluorescenceFriday Jost, Schweizer et al. @ Cuello lab found that a mutation linked to aortic aneurysmas led to distorted actin #cytoskeleton in VSMCs #mechanobiology New collab @cellcommlab.bsky.social by @jboixcampos.bsky.social🔥 Full www.sciencedirect.com/science/arti... #microscopy #cell 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 09/01/2026

#FluorescenceFriday Proud of our the last paper @cellcommlab.bsky.social & 1st one of @jboixcampos.bsky.social We continue to study #CellMigration & how cells decide where to go: ↖️↗️⬇️? (movie of the actin #cytoskeleton) Full www.science.org/doi/10.1126/... @focalplane.bsky.social @science.org 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 01/01/2026

We combined cell biology, #AI based image analysis & a mathematical model to show how migratory behaviour changes with branching For now macrophages & endothelial cells, but we have many more, stay tuned ;) Kudos to all authors for the effort, especially to a great Padawan @jboixcampos.bsky.social

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Pablo J. Sáez @pjsaez.bsky.social · 01/01/2026

#HappyNewYear ! Let's start 2026 with a @cellcommlab.bsky.social paper How many branches is OK during #CellMigration? It depends on the #cell: for ex. immune cells are just fine. In collab. w. #NirGov @hfspo.bsky.social Full www.science.org/doi/10.1126/... @focalplane.bsky.social @science.org 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 04/12/2025

Hot from the press ! Happy to see this out, and that @cellcommlab.bsky.social contributed with live-cell imaging Autoimmunoglobulin-triggered extracellular vesicles (movie) contribute to kidney disease Full www.cell.com/cell/fulltex... @focalplane.bsky.social @cp-cell.bsky.social #microscopy 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 21/11/2025

Even if "provisionally" accepted, those are the best words/news I've heard for quite a while... Here some #blebs to "provisionally" cellebrate. Let's see this #cell dance under the microscope. @focalplane.bsky.social #fluorescencefriday #microscopy 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 14/11/2025

I guess it never gets old...bc we love #CellMigration ATP has many roles: energy currency, hydrotrope, and *danger signal* Immune cells are faster after ATP exposure, F-actin (cyan) goes to the rear for nitro boost! www.science.org/doi/full/10.... @focalplane.bsky.social #fluorescencefriday 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 07/11/2025

I love to see the endoplasmic reticulum (ER, cyan) hitchhiking over microtubules (MTs, orange). Also the membrane dynamics are lovely. It's been a while, but here we are for a late #fluorescencefriday with some #microscopy #scicomm #scisky @cellcommlab.bsky.social focalplane.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 27/10/2025

Kudos to @eliasbarrigalab.bsky.social for the Innovation DGZ Prize for his work on #CellMigration A great Chilean 🇨🇱 in #STEM 💪🏽🔥 @dgz.bsky.social #DGZ50

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Pablo J. Sáez @pjsaez.bsky.social · 01/09/2025

Today 20 years after I started working in a scientific lab while still in 🇨🇱, my lab, the @cellcommlab.bsky.social has its 5th labversary. What a ride ! I'm thankful for the support of my family, friends, & so many great collabs & colleagues. Fig legend 👇🏽 + pending longer thread #PIlife #Science

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Pablo J. Sáez @pjsaez.bsky.social · 31/08/2025

Post an image from your summer that confers peace

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Pablo J. Sáez @pjsaez.bsky.social · 25/07/2025

Barely made ir for another #FluorescenceFriday Ahhh the #Nucleus ... it never gets old. This #cell has 2 bc why not !! It seems they are refusing to separate. This 👇🏽 is an excellent chance to put your last work in the nucleus in this issue edited by @abbybuch.bsky.social @jcellsci.bsky.social

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Pablo J. Sáez @pjsaez.bsky.social · 18/07/2025

#HotFromThePress !! What if instead of analyzing 4-6 pathology markers you could check >100 in the *same* sample? @maltekuehl.com & co @puellesv.bsky.social lab introduce #PathoPlex, method that could even predict disease Full www.nature.com/articles/s41... @focalplane.bsky.social @nature.com 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 08/07/2025

This is why we love #cellmigration #Leukocytes: #Neutrophils (cyan) #Macrophages (orange) chemotaxis. I couldn't share it before the retirement of @zebrafishrock.bsky.social, but cool #microscopy in #zebrafish continue to rock ! #scicomm #scisky @cellcommlab.bsky.social @focalplane.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 29/06/2025

Post yourself from another era. (20 years ago)

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Pablo J. Sáez @pjsaez.bsky.social · 28/06/2025

Do you know what happens if you squeeze a #cell? If the #confinement is high, the nucleus (green) will be super tense & the compressed cells will react like this👇🏽changing their #cytoskeleton (magenta) Full: www.science.org/doi/10.1126/... #scicomm #microscopy #sciencesky @focalplane.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 20/06/2025

#FluorescenceFriday #Mitochondria, powerhouse of the #cell were labeled (cyan) and it's possible to visualize how they reach *every* corner to provide energy. All recorded under the microscope @cellcommlab.bsky.social #scicomm #sciencesky #microscopy #organelles @focalplane.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 07/06/2025

This is why we love #cell dynamics ! Look at this #macrophage branching exploring the surroundings videoed under the microscope This what immune cells do in our body in search of bacteria, viruses and death cells #microscopy #cellbio #sciencesky @focalplane.bsky.social @cellcommlab.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 19/04/2025

100 years ago it was founded the most successful and popular football team in Chile 🇨🇱: #ColoColo ⚽🏆 This year I had the chance to be in, Valladolid, where David Arellano (the the founder of the club) died (Hotel Inglaterra). He died while playing for Colo-Colo. #centenarioalbo #ColoColo100

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Pablo J. Sáez @pjsaez.bsky.social · 22/02/2025

Hot from the press: #CellMigration #vesicles #organelles Jeppesen et al @mag2art.bsky.social lab introduce blebbisomes: organelle-rich motile vesicles, have immune checkpoint inhibitors, released by #cancer cells & used for #cellcomm @naturecellbiology.bsky.social www.nature.com/articles/s41... 🧪🔬

The image shows a figure from a publication introducing a new type of vesicles named blebbisomes.
First light microscopic pictures show forming blebbisomes, then a large zoom on a forming blebbisome Is shown by electron microscopy. Then other pictures show the actin cytoskeleton, and migrating cancer cells that are releasing blebbisomes

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Pablo J. Sáez @pjsaez.bsky.social · 18/02/2025

An oldie (10 y!) but goodie: cellular interaction at the top! #cellcomm Video of a T cell (orange) interacting with a target cell (blue) From Chen et al. @ Betzig lab (@hhmijanelia.bsky.social) game changing paper !! Full science.org/doi/10.1126/... @focalplane.bsky.social #scicomm #microscopy 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 10/02/2025

Hot from the press for #CellMigration + #Nucleus aficionados Karling @helenweavers.bsky.social lab show *in vivo* how immune cell adapt quickly to confined microenvironments during motility. Live-cell imaging, image analysis, #Drosophila ! Full @emboreports.org www.embopress.org/doi/full/10....

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Pablo J. Sáez @pjsaez.bsky.social · 06/02/2025

#CellMigration happens all the time in our bodies. This may (or not) rely on adhesion molecules #Cancer cells switch strategies during metastasis to invade other tissues Naomi Wee (naomiwee.com) did this neat movie to explain it: www.youtube.com/watch?v=D9aD... #SciSky 🧪🔬 @cellcommlab.bsky.social

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Pablo J. Sáez @pjsaez.bsky.social · 01/02/2025

The #nucleus contains the info of a #cell, but it does much more than that !! Since the nucleus is *big*, acts as the main intracellular obstacle, and during #CellMigration can get 'trapped'/stuck until the cell pushes it FWD. #scicomm @focalplane.bsky.social @cellcommlab.bsky.social #Skyscience

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Pablo J. Sáez @pjsaez.bsky.social · 18/01/2025

How cells decide where to go? #CellMigration Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells ) Full www.nature.com/articles/s41... @naturephysics.bsky.social @focalplane.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 13/01/2025

#CellMigration meets #SciArt Naomi Wee (naomiwee.com) did this great movie to explain how amoeboid migration works (also mesenchymal & cancer migration) Wanna know what happens if this leukocyte chooses the wrong path? Watch this ! www.youtube.com/watch?v=mPwn... @focalplane.bsky.social #SciSky

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Pablo J. Sáez @pjsaez.bsky.social · 12/01/2025

#CellMigration aficionados, it would be cool to have a similar video for amoeboid cell migration too, no? Well come tomorrow and see the nice work done by Naomi Wee. Early teaser below, you can already see membrane dynamics and the leading edge vs uropod shape #GRCCellMigration #SciArt #GRC

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Pablo J. Sáez @pjsaez.bsky.social · 10/01/2025

How from the #preprint, super cool story on the role of #cytoskeleton by my former lab Spearheaded by dear friend and cell biologist extraordinaire Mathieu Deygas (Piel, Vargas lab @institutcurie.bsky.social) Data from in vitro to in vivo. Full at www.biorxiv.org/content/10.1... #scicomm #SciSky

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Pablo J. Sáez @pjsaez.bsky.social · 09/01/2025

Warming up for the upcoming #GRCCellMigration For me the best video to explain adhesion-dependent #CellMigration by Felix Donghwi Son More in the website felixvis.artstation.com #sciart #scicomm #ScienceSky #SciSky #cytoskeleton #FocalAdhesion

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Pablo J. Sáez @pjsaez.bsky.social · 07/01/2025

#Organelles closely interacting within a #cell videoed under a microscope Love how the mitochondria (orange) crawl on top, & are almost embedded within the nucleus (cyan) (rendered with ChimeraX @www.cgl.ucsf.edu) @focalplane.bsky.social @dgz.bsky.social @cellcommlab.bsky.social #Scisky #scicomm

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Pablo J. Sáez @pjsaez.bsky.social · 05/01/2025

Some of the ArpC4 KO cells with double nuclei showed in the #preprint, remind me of this one. Frustrated mitosis or something else? #Cell videoed under a microscope #scicomm @focalplane.bsky.social @cellcommlab.bsky.social

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Pablo J. Sáez @pjsaez.bsky.social · 05/01/2025

Interesting study by Delgado et al Lennon lab (@institutcurie.bsky.social), shows that Arp2/3 regulates Langerhans cells & nuclear shape, #cellmigration, and genome stability [In collab with @manellab.bsky.social, Benvenuti, Fachinetti, @sandraiden.bsky.social] Full www.biorxiv.org/content/10.1...

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Pablo J. Sáez @pjsaez.bsky.social · 29/12/2024

Brilliant study by @andreasschoenit.bsky.social et al @ @bladoux.bsky.social lab Force competition at the cellular level. #mechanobiology

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Pablo J. Sáez @pjsaez.bsky.social · 27/12/2024

Biology is amazing (wait for it): 1 ant cannot solve the task, which forces them to collaborate efficiently. 1 human can solve it, therefore no significant increase of working in groups, & *if* the group cannot communicate they become a "dumb crowd". by Dreyer et al.: www.pnas.org/doi/abs/10.1...

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Pablo J. Sáez @pjsaez.bsky.social · 27/12/2024

Last #fluorescencefriday of the year, let's finish with some more #CellMigration ! More immune cells migrating within microchannels. Which LUT do you prefer? @focalplane.bsky.social @cellcommlab.bsky.social 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 20/12/2024

ATP acts as i) cellular energy currency, ii) danger signal, & much more ATP induces fast migration of leukocytes, w depends on P2X7, Panx1 channels, & CaMKII, w leads to actin cytoskeleton (cyan) changes www.science.org/doi/full/10.... @focalplane.bsky.social #CellMigration #fluorescencefriday 🧪🔬

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Pablo J. Sáez @pjsaez.bsky.social · 18/12/2024

The AI revolution. Google Veo-2 Vs OpenAI Sora

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