The Department of Biology at Stanford University seeks applicants for a tenure-track Assistant Professor in Evolutionary Biology. A successful candidate will conduct research that asks fundamental que...

Biayna, Baranovskii and colleagues report that intron-retained RNAs derived from cell cycle- and genome maintenance-related genes are pooled at the periphery of nuclear speckles and undergo synchronou...

Abstract. Changes in regulatory sequences controlling the timing and activity of gene products underlie much of natural phenotypic variation. Yet, identify

Advances in mitochondrial genome editing have expanded efforts to manipulate mitochondrial DNA and model mitochondrial disease. This Review discusses the development of programmable nucleases and mitochondrial base editors, their applications in disease modelling and therapeutic correction, and the key challenges to clinical translation.

This study introduces iChIP2, a low-input chromatin immunoprecipitation followed by sequencing method that profiles histone post-translational modifications (hPTMs) simultaneously across diverse eukar...

A nanopore-based ‘chop and measure’ method sequences peptides at single-amino-acid resolution by using enzymatic digestion to progressively shorten the N terminus one residue at a time,...

Nature - A newly compiled atlas of species-wide structural variants and gene-based and graph pangenomes derived from highly complete assemblies of genomes from 1,086 natural isolates enable...

Molecular Biosystems Conference

Read this JoVE article on In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Molecular Biosystems Conference

Traditionally, antifungal resistance (AFR) has received much less attention compared with bacterial resistance to antibiotics. However, global changes, pandemics, and emerging new fungal infections have highlighted global health consequences of AFR. The recent report of the World Health Organisation (WHO) has identified fungal priority pathogens, and recognised AFR among the greatest global health threats. This is particularly important given the significant increase in fungal infections linked to climate change and pandemics. Environmental factors play critical roles in AFR and fungal infections, as many clinically relevant fungal pathogens and AFR originate from the environment (mainly soil). In addition, the environment serves as a potential rich source for the discovery of new antifungal agents, including mycoviruses and bacterial probiotics, which hold promise for effective therapies. In this article, we summarise the environmental pathways of AFR development and spread among high priority fungal pathogens, and propose potential mechanisms of AFR development and spread. We identify a research priority list to address key knowledge gaps in our understanding of environmental AFR. Further, we propose an integrated roadmap for predictive risk management of AFR that is critical for effective surveillance and forecasting of public health outcomes under current and future climatic conditions.

Controlling for phylogeny is essential in comparative genomics studies, because species, genomes and genes are not independent data points within statistical tests. The authors review the application ...

Ecological interactions are central to our understanding of the composition and function of communities, but attempts to use them as a theoretical foundation for experimental ecology have been confou....

Phylowave, an innovative phylogenetic approach, can identify the main circulating pathogen lineages with increased fitness and the associated genetic changes, enabling the timely identification of…

Michael Elowitz on the early years of synthetic biology and the programmability of living cells.

Jove

Abstract. Ecological theory predicts that organismal distribution and abundance depend on the ability to adapt to environmental change. It also predicts th

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy).Concerns have bee…

The Plant Cell publishes novel research of special significance in plant biology, especially in the areas of cellular biology, molecular biology, biochemistry, genetics, development, and evolution.

A streamlined CRISPR-Cas9-based toolbox that improves the engineering process of Komagataella phaffii yeast by implementing short single-stranded DNA seque

npj Antimicrobials and Resistance - Unlocking the potential of experimental evolution to study drug resistance in pathogenic fungi

glabrata is an opportunistic pathogen that can resist common antifungals and rapidly acquire multidr

During mixed-species invasive candidiasis - an emerging threat in healthcare - presence of Candida a

Candida glabrata is a fungal microbe associated with multiple vertebrate microbiomes and their terrestrial environments. In humans, the species has emerged as an opportunistic pathogen that now ranks ...

Recent years have seen intense interest in the development of point-of-care nucleic acid diagnostic technologies to address the scaling limitations of laboratory-based approaches. Chief among these are combinations of isothermal amplification approaches with CRISPR-based detection and readouts of target products. Here, we contribute to the growing body of rapid, programmable point-of-care pathogen tests by developing and optimizing a one-pot NASBA-Cas13a nucleic acid detection assay. This test uses the isothermal amplification technique NASBA to amplify target viral nucleic acids, followed by the Cas13a-based detection of amplified sequences. We first demonstrate an in-house formulation of NASBA that enables the optimization of individual NASBA components. We then present design rules for NASBA primer sets and LbuCas13a guide RNAs for the fast and sensitive detection of SARS-CoV-2 viral RNA fragments, resulting in 20–200 aM sensitivity. Finally, we explore the combination of high-throughput assay condition screening with mechanistic ordinary differential equation modeling of the reaction scheme to gain a deeper understanding of the NASBA-Cas13a system. This work presents a framework for developing a mechanistic understanding of reaction performance and optimization that uses both experiments and modeling, which we anticipate will be useful in developing future nucleic acid detection technologies.