Automated cryo-EM 2D class evaluation and sorting with AI.

CryoSift is a CNN-based tool that assesses the quality of 2D class averages. The incorporation of CryoSift into cryoSPARC workflows enables automated particle-stack cleaning.

The development of methods for low-cost time-resolved cryo-EM using an on-grid mixing strategy is described.

Critical Thinking AI for constructive criticism and science evaluation

This work presents a new cyberinfrastructure framework for cryo-EM structure determination, Magellon, which offers a robust and flexible approach to cryo-EM data management. We show the power of this ...

The molecular comparison of related RabGEFs reveals adaptation mechanisms of a functional module for specific cellular tasks.

Disulfide-based dimerization of modified identical and heterologous nanobody scaffolds enables higher-order assembly for high-resolution cryo-electron microscopy structure determination that is widely...

Lithium has an essential role in the brain and is deficient early in Alzheimer’s disease, which can be recapitulated in mice and treated with a novel lithium salt that restores the physiological level.

Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report s…

AlphaFold - A practical guide