iiinova — where your digital self keeps evolving

Lander Lab @landerlab.bsky.social · 01/05/2025

Our latest review is out in COSB! Led by Zeba Rizvi @zrizvi.bsky.social,‬ we explore how cryo-EM has brought new insights to Cullin RING ligases - from conformations to allosteric control, cryo-EM has massively improved our understanding of these dynamic molecular machines. tinyurl.com/y4sza8dh

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Lander Lab @landerlab.bsky.social · 21/03/2025

Big congrats to Dr. Kelly Chen on completing her PhD! She wrangled human ClpXP into submission with CryoEM, and now she's off to tackle new challenges at Genentech. We couldn’t be more proud of all she’s accomplished, and we all wish her all the best in this next chapter of her scientific journey.

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Lander Lab @landerlab.bsky.social · 19/03/2025

That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!

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Lander Lab @landerlab.bsky.social · 19/03/2025

Congrats to Group 1, winners of the 2025 CSHL #CryoEM course! They reconstructed a 1.9 A structure from 100 images, the built and refined an outstanding atomic model with great validation metrics. They are the recipients of the new Cryo Champion Chalice, adorned with $2,467 worth of used autogrids!

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Lander Lab @landerlab.bsky.social · 18/03/2025

CSHL 2025 #CryoEM course update - final hour of the competition and the teams are refining their atomic models. Amazing what these students have done with 100 micrographs in just a few hours!

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Lander Lab @landerlab.bsky.social · 18/03/2025

It’s the last full day of the CSHL #CryoEM course! Last leg of the competition - solve the structure of a mystery protein and generate an atomic model with a complete Table 1 (sequence not provided). Students are learning that ChatGPT isn’t much help when trying to identify symmetry of a structure…

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Lander Lab @landerlab.bsky.social · 18/03/2025

Brace yourselves, Matthijn Vos @matthijnvos.bsky.social has arrived! EM expert & head of the NanoImaging Core at the Institut Pasteur, he's known for his razor-sharp critiques and brutal honesty. But beneath the grumpiness? A brilliant scientist and an invaluable colleague. Welcome, Matthijn!

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Lander Lab @landerlab.bsky.social · 17/03/2025

Happy St. Patrick’s Day from the CSHL #cryoEM course! Data collection day for the final competition, but it looks like some mischievous leprechauns got into the microscope and messed up the autoloader… A tough but valuable lesson for the students: sometimes your resolution is limited by bad luck…

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Lander Lab @landerlab.bsky.social · 17/03/2025

Day 11 of the CSHL #CryoEM course: depositing data and new developments at the EMDB with Jack Turner, @jencash.bsky.social showed how to make graphene oxide grids, and learned how to make local resolution maps. Competitive data collection starts tomorrow!

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Lander Lab @landerlab.bsky.social · 16/03/2025

We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!

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Lander Lab @landerlab.bsky.social · 16/03/2025

Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.

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Lander Lab @landerlab.bsky.social · 14/03/2025

Outstanding lecture on EM validation by Oli Clarke @olibclarke.bsky.social this morning at the CSHL #cryoEM course.

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Lander Lab @landerlab.bsky.social · 14/03/2025

Today at the CSHL #CryoEM course: we began with an excellent overview of atomic model validation by Christopher Williams, then an in-depth modeling tutorial by @jencash.bsky.social, a masking and local refinement tutorial by Kelli H, and tonight the students begin flying solo on the Krios!

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Lander Lab @landerlab.bsky.social · 12/03/2025

Our first competition at the CSHL #CryoEM course - each student tries to make a perfect grid of ryanodine receptors. We’ll be checking atlases after dinner… who will be tonight’s big winner? Thanks to @olibclarke.bsky.social for providing sample and gold grids!

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Lander Lab @landerlab.bsky.social · 11/03/2025

Day 6 of the CSHL #CryoEM course: field trip to the city! Visited the New York Structural Biology Center, where Ed Eng @edwardteng.bsky.social introduced everyone to the EM sample prep and collection resources available at nccat.nysbc.org. Then a tour of the new technologies being developed there!

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Lander Lab @landerlab.bsky.social · 11/03/2025

Day 5 of the CSHL #cryoEM course: clipping and loading autogrids. Thanks to SubAngstrom for supplying us with rings and clips for the course! Kelli H also gave an amazing tutorial on 3D mask creation, which we put to use for a signal subtraction tutorial run tonight by Katerina Naydenova.

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Lander Lab @landerlab.bsky.social · 09/03/2025

Day 4 of the CSHL #CryoEM course - A great lecture on advanced processing strategies from Justin Kollman, then more hands-on training! Freezing cryo samples with the Leica GP2, TFS Vitrobot, and manual plunger; preparing and checking negative stain grids; and checking alignments on the Krios.

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Lander Lab @landerlab.bsky.social · 08/03/2025

Everyone crowding around Matthijn Vos for an introductory demo on the Krios on day 3 of the CSHL #CryoEM course

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Lander Lab @landerlab.bsky.social · 08/03/2025

Today at the CSHL #CryoEM course Lauren @lalexandrescu.bsky.social showed everyone how to make their own Quantifoil-style holey cryo-em grids! Thanks to @johnrubinstein.bsky.social for introducing us to this technique - I predict it will be adopted by many other labs!

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Lander Lab @landerlab.bsky.social · 07/03/2025

We couldn't get out to #StandUpForScience with everyone else, but lots of great science discussed today at the CSHL #CryoEM course - intro to electron microscope optics with Alex de Marco, Fourier transforms & CTF with Tim Grant, and direct detectors with Katerina Naydenova.

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Lander Lab @landerlab.bsky.social · 07/03/2025

BTW welcome to Bluesky, Yifan Cheng @cryosp.bsky.social Professor at UCSF, HHMI investigator, #CryoEM pioneer, and aggregator of fish, chicken, and cannolis on a single plate.

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Lander Lab @landerlab.bsky.social · 07/03/2025

Amazing Day 1 of the CSHL #CryoEM Course! Yifan Cheng @cryosp.bsky.social kicked things off with a historical perspective and discussing dynamic allostery, Kelli Hvorecny gave a workflow overview, Lauren Anderson introduced ChimeraX @www.cgl.ucsf.edu, and Katerina Naydenova ran a RELION tutorial.

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Lander Lab @landerlab.bsky.social · 06/03/2025

Welcome to the participants of the 2025 #CryoEM course at Cold Spring Harbor Lab @cshlaboratory.bsky.social ! It's going to be a great 2 weeks immersed in all aspects of single particle cryoEM methods.

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Lander Lab @landerlab.bsky.social · 03/03/2025

Congrats to our postdoc Jan-Hannes Schaefer @jhschaef.bsky.social on being awarded a fellowship from the Walter Benjamin Program! Jan is collaborating with the Kelly lab here at Scripps Research @scripps.edu to understand how transthyretin forms amyloids that lead to heart disease.

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Lander Lab @landerlab.bsky.social · 03/03/2025

Congrats to Chris Zerio on publication of his study showing how cancer-associated Polymerase Theta undergoes both composition and conformational rearrangements to bring together broken strands of DNA for repair. Great collaboration with MOMA therapeutics. Now in peer-reviewed form: rdcu.be/ebFya

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Lander Lab @landerlab.bsky.social · 22/01/2025

Just because it's a homo-oligomer, don't assume it's symmetric! @benjabsnt.bsky.social & collaborators used cryo-EM to study the transthyretin (TTR) tetramer (55 kDa), shedding light on its misfolding pathways linked to amyloid disease. Now in peer-reviewed form: www.nature.com/articles/s41...

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Lander Lab @landerlab.bsky.social · 16/01/2025

Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...

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Lander Lab @landerlab.bsky.social · 19/12/2024

A huge week for Prakhyat, our joint @scripps.edu student with the @wisemanlab.bsky.social! He was awarded an AHA earlier in the week and today he passed his PhD candidacy exam! Because Prakh is so chill, we celebrated with chocolate-coffee liquid nitrogen ice cream. 😎

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Lander Lab @landerlab.bsky.social · 17/12/2024

Congrats to our amazing @scripps.edu grad student Prakhyat Mathur (joint with @wisemanlab.bsky.social) for being awarded a predoctoral fellowship from the AHA @americanheart.bsky.social. Prakh will be investigating how mitochondrial stress activates the integrated stress response. Well-earned Prakh!

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Lander Lab @landerlab.bsky.social · 04/12/2024

Congrats to our grad students who pitched therapeutic targets at this year's @scripps.edu Drug Discovery Shark Tank - particularly Shelby @shelbyferrier.bsky.social and Morgan @morganmgee.bsky.social on their winning proposal to target Cholera toxin subunit B, which raised $4 million!

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Lander Lab @landerlab.bsky.social · 22/11/2024

Electrons + Photons in a TEM = a BRIGHTER future for single particle? Cool preprint from #EPFL - they melted & re-vitrified samples with a laser inside the TEM. Not convinced this is the solution to overcoming preferred orientation, but pioneering work nonetheless. www.biorxiv.org/content/10.1...

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Lander Lab @landerlab.bsky.social · 14/11/2024

The human mitochondrial ClpXP is uniquely assembled and regulated compared to its bacterial counterparts. Congrats to Kelly Chen on her years of hard work on this challenging complex! www.biorxiv.org/content/10.1...

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