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matthijnvos.bsky.social

@matthijnvos.bsky.social

113 followers 202 following 0 posts

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Ariane Briegel @arianebriegel.bsky.social · 09/06/2025

I have a question for all CryoEM folks here- has anyone done bsl2 pathogen survival tests after a cryocycle? Please let me know if anyone has any insights on this 🙏🙏🙏

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Saganism @sagan.bsky.social · 06/05/2025

Carl Sagan on sensible military spending and climate change.

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Ben Engel @cellarchlab.com · 06/05/2025

Thanks a lot @arianebriegel.bsky.social for visiting the @biozentrum.unibas.ch and telling us about your amazing #TeamTomo science. The squid 🦑 bacterial symbiosis cryo-lift out is super cool!! 🤩 #FIBtheWorld 🧪🧶🧬🌊

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PLOS Biology @plosbiology.org · 02/05/2025

How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z

Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.

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