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Eva Maria Novoa

@evamarianovoa.bsky.social
697 followers 154 following 15 posts

ICREA Professor & Group Leader - Epitranscriptomics & RNA Dynamics @CRGenomica. Biochemist and bioinformatician. #RNAmods #nanopore #ribosome #tRNA #inheritance. Mom of 3. Piano, beach vball, hiking Website: novoalab.com

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Eva Maria Novoa @evamarianovoa.bsky.social · 20/08/2026
Need to #multiplex your #nanopore direct RNA runs? We are happy to announce an upgraded version of #SeqTagger #version2 with demuxing models for #mRNA (compatible with polyadenylated #rRNA) and #tRNA. Code and models publicly available in GitHub! github.com/novoalab/Seq...
github.com
GitHub - novoalab/SeqTagger: Super-fast and accurate demultiplexing of direct RNA-seq runs (Pryszcz*, Diensthuber*, et al., Genome Res 2025)
Super-fast and accurate demultiplexing of direct RNA-seq runs (Pryszcz*, Diensthuber*, et al., Genome Res 2025) - novoalab/SeqTagger
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Eva Maria Novoa @evamarianovoa.bsky.social · 04/03/2026
Please check out our latest #preprint to study #ACTIVELY TRANSLATING #tRNA populations and their dynamics upon #stress :) This was an wonderful collaborative effort between @novoalab.bsky.social @immagina.bsky.social and #Soares teams - thank you for making this possible!
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Novoa Lab @CRG @novoalab.bsky.social · 10/11/2025
It was our pleasure to host @james-bryson.bsky.social (BRIC, Copenhagen) at @crg.eu through the EU-LIFE Postdoctoral Exchange Program! Big thanks to James for presenting his work on advanced CRISPR tools for epitranscriptomic research, and to @eu-life.bsky.social for this opportunity!🧬✈️ #EULIFE #CRG
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Novoa Lab @CRG @novoalab.bsky.social · 14/10/2025
🧬 New review on nanopore basecalling models is out! ✨ Congrats to @soniacruciani.bsky.social (former Novoa Lab PhD, now postdoc at the Deplancke Lab, EPFL) and @evamarianovoa.bsky.social👏 📖 www.nature.com/articles/s41...
nature.com
Client Challenge
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Eva Maria Novoa @evamarianovoa.bsky.social · 03/08/2025
Fresh preprint from the @novoalab.bsky.social !😀📌 Do you want to barcode up to 96 #RNA samples in your #nanopore flowcells? How well do RNA #modification-aware models perform? What if there is #noBasecallingModel for your modification-of-interest? Find it out here!😊 www.biorxiv.org/content/10.1...
biorxiv.org
Systematic benchmarking of basecalling models for RNA modification detection with highly-multiplexed nanopore sequencing
Nanopore direct RNA sequencing (DRS) holds promise for advancing our understanding of the epitranscriptome by detecting RNA modifications in native RNA molecules. Recently, Oxford Nanopore Technologie...
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Gregor Diensthuber @gdiensthuber.bsky.social · 14/07/2025
🚨 New preprint alert 🚨 We systematically benchmarked @nanoporetech.com 's modification-aware basecalling models released for RNA on sets of in vitro and in vivo sequences and made some curious observations 🧬🔍. bit.ly/4lXqNul Follow along for a little recap (1/12)
bit.ly
Systematic benchmarking of basecalling models for RNA modification detection with highly multiplexed nanopore sequencing
Nanopore direct RNA sequencing (DRS) holds promise for advancing our understanding of the epitranscriptome by detecting RNA modifications in native RNA molecules. Recently, Oxford Nanopore Technologie...
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Monica Bettencourt-Dias @monicabettencourt.bsky.social · 01/07/2025
Happy to be the next (and 1st female) @crg.eu director. The CRG always stood out to me, for its excellence in understanding life's principles, with implications for health and biodiversity, & its collaborative, open and innovative way of doing science. Thrilled to join its amazing community in 2026!
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Novoa Lab @CRG @novoalab.bsky.social · 28/02/2025
New manuscript from the Novoa lab was published this week in Genome Biology! You can give it a read here: rdcu.be/ebiIs
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Crystal Rogers, PhD @rogerslabucd.bsky.social · 19/02/2025
Pretty sad. Just got the email that CZI is canceling the second round of Diversity Leadership Awards. Private industry will definitely not fill the hole that NIH and NSF are leaving. 😢💔
Thank you for submitting your Letter of Intent (LOI) to CZI. We appreciate your interest in our work.

CZI has decided not to continue with the second round of its Science Diversity Leadership Awards. We are committed to supporting groundbreaking research that advances the frontiers of scientific knowledge in pursuit of our mission to cure, prevent, or manage all diseases by the end of the century. We will provide information on future funding opportunities.

We look forward to staying in touch.

Bil Clemons, Program Officer, CZI
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Nat Trigg @natrigg.bsky.social · 16/02/2025
Working with germ-free mice with the Kambayashi Lab we saw a 'swollen' epididymal phenotype occurring, so we took a closer look doi.org/10.1530/REP-...
doi.org
A lack of commensal microbiota influences the male reproductive tract intergenerationally in mice
The microbiome encompasses the array of microorganisms inhabiting various niches in the body and is necessary for numerous physiological processes, including normal metabolism and a functioning immune...
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Eva Maria Novoa @evamarianovoa.bsky.social · 28/01/2025
Are #rRNA #modifications equal a across cell types, conditions and #disease? Are they tuned upon #antibiotics exposure? In our recent works, we find that yes and yes! Exciting times ahead!! Please see authors.elsevier.com/c/1kF253vVUP... and www.nature.com/articles/s41... Feedback very welcome!! :)
authors.elsevier.com
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Karousis Lab @karousislab.bsky.social · 18/12/2024
New pre-print 📢:Cell-free translation from diverse human cell types: Fast, reproducible and scalable t.co/IAld0ocjDf @nickkouvelas.bsky.social @unibern.bsky.social #biorxiv #mRNA #Ribosome
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Eva Maria Novoa @evamarianovoa.bsky.social · 22/01/2025
How has native RNA sequencing contributed to epitranscriptomic research? This is one of the main questions we addressed in our recent review with @gdiensthuber.bsky.social Now live! authors.elsevier.com/a/1kS2f3vVUP...
authors.elsevier.com
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Claus Wilke @clauswilke.com · 06/12/2024
This is a good recommendation. In addition, for bar plots or other plots where you're coloring large areas, add some transparency. The viridis colors are too dark and saturated for large areas. They were designed for points and lines(*). Compare left versus right.
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Eva Maria Novoa @evamarianovoa.bsky.social · 21/11/2024
Very happy to share our updated #Nano3P-seq protocol, adapted to #nanopore R10 chemistry. After a lot of troubleshooting both in the wet lab protocol but also the computational analysis pipeline, we finally made it! :-) www.biorxiv.org/content/10.1...
biorxiv.org
Nano3P-seq: charting the coding and non-coding transcriptome at single molecule resolution
RNA polyadenylation is crucial for RNA maturation, stability and function, with polyA tail lengths significantly influencing mRNA translation, efficiency and decay. Here, we provide a step-by-step pro...
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Gang Fang @gangfang.bsky.social · 20/11/2024
(1st post @BlueSky) Preprint alert🚨a long thread. Cautions in the use of @nanopore sequencing to map DNA modifications: officially reported “accuracy” ≠ reliable mapping in real applications. We performed a critical assessment of nanopore sequencing (across different versions of models) for the 1/n
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Itai Yanai @itaiyanai.bsky.social · 19/11/2024
I must admit that this annotated Nature abstract remains a useful recipe for constructing a summary paragraph. I show it to my students every time we get started.
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