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Janine Brunner

@janinebrunner.bsky.social
210 followers 305 following 8 posts

Group Leader VIB and Assistant Professor VUB Brussels, Belgium - Biochemist - Structural biologist - interested in #MembraneProteins #cryoEM - nature - paragliding

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Janine Brunner @janinebrunner.bsky.social · 03/04/2026
Out at Nat Commun: Stereoselectivity and plasticity of a common binding pocket in TRPM3 One pocket, many ligands. CryoEM & electrophysiology reveal a promiscuous site where mutations rewire stereoselectivity and invert the functional outcome of ligand binding nature.com/articles/s41467-026-71226-0
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Andrew Plested @andrewplested.bsky.social · 15/11/2025
Game changer, no doubt. Commercial systems will have expensive consumables I suppose, but huge savings to be made in cell culture, and many important but sparse native targets come into play. Crispr-tagged targets from iPSC neurons, for example. TRPC6 blind? 🤯
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Janine Brunner @janinebrunner.bsky.social · 14/11/2025
MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony. Or from a single dish of HEK cell culture in the case of two membrane proteins. Out in Nature Methods now! lnkd.in/gpyBSceg Wonderful collaboration with the Efremov lab.
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John Rubinstein @johnrubinstein.bsky.social · 30/10/2025
New lab preprint! @zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes 🧵 tinyurl.com/ukny4ptz
Cryo-EM maps and atomic models of the biotin-containing 3-methylcrotonyl-CoA carboxylase (MCC) complex and long-chain acyl-CoA carboxylase (LCC) complex
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Harry Low’s Lab @lowlab.bsky.social · 29/10/2025
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems. For more details and to apply please see www.imperial.ac.uk/jobs/search-...
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Janine Brunner @janinebrunner.bsky.social · 28/08/2025
Happy to announce our most recent preprint: Stereoselectivity and plasticity of a common binding pocket in TRPM3, by cryo-EM and elecrophysiology. Great collaboration with the Voets, Vriens and CISTIM labs. www.biorxiv.org/content/10.1...
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Vanni Lab at UNIFR, Switzerland @labvanni.bsky.social · 07/08/2025
Happy to share the latest from the lab, led by Daniel Alvarez, in collaboration with @lizconibear.bsky.social‬. In this AA-MD tour-de-force, we delve deep into the mechanism and energetics of lipid uptake by bridge-like lipid transfer proteins, and we learn a few interesting things along the way...
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A. Reza Mehdipour @rezamehdipour.bsky.social · 05/08/2025
We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!! www.biorxiv.org/content/10.1...
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Vanni Lab at UNIFR, Switzerland @labvanni.bsky.social · 23/07/2025
Happy to share the latest work from the lab, led by @mudgal17.bsky.social‬, in collaboration with the Weis lab @ethzurich.bsky.social. How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)
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Del Mármol lab @delmarmollab.bsky.social · 03/06/2025
New preprint in the lab combining what we like best: insects, love, chemistry and the olfactory system! 1/8 www.biorxiv.org/content/10.1...
biorxiv.org
A covalent recognition strategy enables conspecific mate identification
The olfactory system can detect an uncountable number of volatile molecules while retaining the ability to discriminate amongst very similar ones. We identified a unique mechanism employed by insect o...
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Jie Xu @jie-xu.bsky.social · 09/06/2025
Excited to share our latest story in Nature. We developed a high-throughput cellular stretch system to look for genes regulating membrane rupture under tension, and found an amazing small protein called NINJ1 that weakens the membrane for rupture! H/T to Zozo, my 10-y.o. for her artist's impression😃
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Ardem Patapoutian @ardemp.bskyverified.social · 09/06/2025
Check out this remarkable debut paper from @jie-xu.bsky.social, a very creative lab alumnus. Very proud! www.nature.com/articles/s41...
nature.com
NINJ1 regulates plasma membrane fragility under mechanical strain - Nature
Nature - NINJ1 regulates plasma membrane fragility under mechanical strain
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Adrià Sogues @adriasogues.bsky.social · 20/05/2025
I’m thrilled to finally share this preprint! It contains a WOW Cryo-EM structure (likely one of the strongest protein fibers known to man 🤯), but we’ve also untangled its biological function: a novel type of virulence factor💀! www.researchsquare.com/article/rs-6...
researchsquare.com
Auto-crosslinking sporesilk fibers promote endospore and Cry toxin clustering
Bacillus thuringiesis infects and kills insect larvae via its spores and associated entomotoxin-containing parasporal bodies (PSBs). We show that endospores and PSBs of B. thuringiensis Sv. Israë...
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mikesleutel.bsky.social @mikesleutel.bsky.social · 20/05/2025
In our latest preprint we used cryoEM to solve the structure of A-ENA fibers and show that they are stabilized by 10 isopeptide bonds per monomer. A-ENA couples the spore to the cry-toxins, and in doing so increases the virulence of Bacillus thuringiensis: www.researchsquare.com/article/rs-6...
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Janine Brunner @janinebrunner.bsky.social · 05/05/2025
I am delighted to share our findings on the binding mode of tetanus neurotoxin and the anti-epileptic drug Levetiracetam to SV2A, an essential synaptic vesicle membrane protein of unknown function, now online in @NatComms.bsky.social   www.nature.com/articles/s41...
nature.com
Structures of native SV2A reveal the binding mode for tetanus neurotoxin and anti-epileptic racetams - Nature Communications
SV2A, an essential membrane protein in neurotransmission, is known as the receptor of clostridial neurotoxins and target of anti-epileptic drugs. Here the authors show details of the binding site for ...
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Harry Low’s Lab @lowlab.bsky.social · 28/03/2025
Please RT. Post-doc opportunity alert! 💥 Come join our team (thelowlab.org) at Imperial, London, working on bacterial secretion systems. The position is funded by the Wellcome Trust. For more details and to apply please see www.imperial.ac.uk/jobs/search-...
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Ed Twomey @cryoet.bsky.social · 26/03/2025
Here's our newest study: we reveal the molecular principles of neuronal excitation by glutamate, and how physiological temperatures influence this process. www.nature.com/articles/s41... www.nature.com/articles/d41... Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com
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cryoEM papers @cryoempapers.bsky.social · 18/03/2025
Structural basis of ClC-3 inhibition by TMEM9 and PI(3,5)P <sub>2</sub> pubmed.ncbi.nlm.nih.gov/40093093/ #cryoEM
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Chao Qi @qcxy8886.bsky.social · 13/03/2025
Happy to share our latest publication on @naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W. thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳 rdcu.be/edqzW rdcu.be/edoGv
rdcu.be
Mutant tau filaments from some inherited frontotemporal dementias show the Alzheimer fold
Nature Structural & Molecular Biology - Frontotemporal dementias can be caused by mutations in MAPT, which encodes the protein tau; the mutant protein forms harmful aggregates in the brain....
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Sylvie Callegari @sylviecallegari.bsky.social · 13/03/2025
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/... @wehi-research.bsky.social @komanderlab.bsky.social
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Waggoner Lab @labwaggoner.bsky.social · 23/02/2025
Themis and Grb2 form a constitutive structural hub in TCR signaling doi.org/10.1101/2025... @savvideslab.bsky.social
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Lander Lab @landerlab.bsky.social · 22/01/2025
Just because it's a homo-oligomer, don't assume it's symmetric! @benjabsnt.bsky.social & collaborators used cryo-EM to study the transthyretin (TTR) tetramer (55 kDa), shedding light on its misfolding pathways linked to amyloid disease. Now in peer-reviewed form: www.nature.com/articles/s41...
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Beck Laboratory @becklab.bsky.social · 17/01/2025
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
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Lander Lab @landerlab.bsky.social · 16/01/2025
Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...
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Janine Brunner @janinebrunner.bsky.social · 16/01/2025
Cryo-EM structures of mammalian membrane proteins from a single cell culture dish? Yes, Micro ISOlation (MISO) sample preparation makes it possible. www.biorxiv.org/content/10.1... Happy to be part of this exciting story and wonderful collaboration with the Rouslan Efremov lab.
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Harry Low’s Lab @lowlab.bsky.social · 15/01/2025
Love this.. 👏 MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony www.biorxiv.org/content/10.1...
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