Sign in

mikesleutel.bsky.social

@mikesleutel.bsky.social
19 followers 16 following 2 posts
PostsRepliesMedia
Reposted by @mikesleutel.bsky.social
Adrià Sogues @adriasogues.bsky.social · 27/04/2026
This one deserves it🤩! We used cryo‑EM to identify a new “sporesilk” in Bacillus thuringiensis. The fiber, called A‑ENA, forms an incredibly stable covalent network that increases virulence by keeping the toxic payload and spores in a biofilm-like structure. www.nature.com/articles/s41...
nature.com
Auto-crosslinking sporesilk fibers promote endospore and Cry toxin clustering - Nature Communications
Authors reveal that protein nanofibrils govern assembly of bacterial spores and extrasporal toxins into insecticidal aggregates. Spores and parasporal bodies are incased in a “sporesilk” matrix that d...
12510
Reposted by @mikesleutel.bsky.social
vinceconticello.bsky.social @vinceconticello.bsky.social · 05/01/2026
New manuscript alert. Turns out that calcium ion coordination may be a more common mechanism than we initially expected to trigger polymerization of extracellular filaments. @mikesleutel.bsky.social @vikramalva.bsky.social www.biorxiv.org/content/10.6...
biorxiv.org
From fibril to framework: P. abyssi AbpX illuminates a calcium-responsive family of microbial biomatrix proteins that form thermostable hydrogels
Evolutionary pressure on microbial communities propagating under extreme environmental conditions often results in unique structural adaptations to promote cell survival. Here, we report an investigat...
175
mikesleutel.bsky.social @mikesleutel.bsky.social · 16/08/2025
For over 100 years, Bt spores have been used to protect crops from caterpillars. These spores bear mysterious hair-like ENAs. Thanks to cryoEM, we now know ENAs are robust protein fibers that cluster spores like grappling hooks. Read more in Nature Communications. www.nature.com/articles/s41...
nature.com
Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes - Nature Communications
B. thuringiensis spores contain uncharacterized protein filaments that extend from the surface of the exosporium. Here, the authors show that these filaments feature conserved β-barrel neck domains an...
061
Reposted by @mikesleutel.bsky.social
Adrià Sogues @adriasogues.bsky.social · 30/07/2025
Happy to see the final version out! Cryo-EM structure of the PS2 S-layer in Corynebacterium 🔬! We show it provides mechanical support to the cell wall and assembles at the poles in coordination with PG. It can also be used for covalent surface display. www.pnas.org/doi/10.1073/... #microsky
pnas.org
Cryo-EM structure and polar assembly of the PS2 S-layer of Corynebacterium glutamicum | PNAS
The polar-growing Corynebacteriales have a complex cell envelope architecture characterized by the presence of a specialized outer membrane compose...
1142
mikesleutel.bsky.social @mikesleutel.bsky.social · 20/05/2025
In our latest preprint we used cryoEM to solve the structure of A-ENA fibers and show that they are stabilized by 10 isopeptide bonds per monomer. A-ENA couples the spore to the cry-toxins, and in doing so increases the virulence of Bacillus thuringiensis: www.researchsquare.com/article/rs-6...
13712