Plasmid construction underpins molecular biology and synthetic biology, yet validation is often limited to the inserted fragment rather than the whole plasmid, and around a third of laboratory-made pl...

Plasmid Integrity by Complete Assembly and Rapid Determination sequencing (PICARD-seq) using ONT MinION. . Read full protocol, steps, and materials on protoc...

Cell-free is a powerful platform to unravel biological complexity. Here, the authors used active learning to optimize buffer composition, boosting mRNA 20-fold, enabling cell-free transcriptomics and ...

Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...

Precise timing of gene expression is a common feature in natural regulatory networks but is rarely implemented in synthetic pathways, where static control often limits performance. Here, we expand the...

This protocol details the design principles and procedures for building programmable CRISPRi-based gene circuits, and for testing individual components and the assembled circuits in various plant spec...

Long-read sequencing in 18 unicellular eukaryotes reveals that 6mA is widespread across eukaryotes and is enriched at transcriptionally permissive regions, which are also marked by H3K4me3.

Genetic circuits rely on modular, well-characterized genetic parts to achieve predictable cellular behaviour. Despite their widespread adoption, biological parts are complex and when used in a new mol...

Abstract. Cytosine DNA methylation patterns vary widely across eukaryotes, with its ancestral roles being understood to have included both transposable ele

Programmable and reversible CRISPRi-based genetic circuits function in a variety of plants.

Plant diversity and synthetic biology offer solutions to the global challenge of crop protection Non-flowering plants such as liverworts, mosses, and ferns belong to divergent lineages with ancient…

Synthetic gene circuits have the potential to revolutionise how plants can be engineered by allowing highly customised gene expression patterns to be designed. Gene circuits can sense and integrate m....

CRISPR-Cas technology has revolutionized biology by enabling precise DNA and RNA edits with ease. However, significant challenges remain for translating this technology into clinical applications. Tra...

Synthetic gene circuits in plants: recent advances and challenges - Volume 6

Synthetic gene circuits in plants: recent advances and challenges - Volume 6

Synthetic gene circuits in plants: recent advances and challenges - Volume 6

Mapping enhancers and their target genes in specific cell types is crucial for understanding gene re

A deep learning platform for structure-guided, generative design of RNA sequences is developed and used to discover fluorescent RNA aptamers.

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A major goal in synthetic development is to build gene regulatory circuits that control patterning. Here the authors discover that elevated cell density dampens SynNotch signaling, enabling the design...