Coenzyme A (CoA) is an essential metabolic cofactor whose biosynthesis is controlled by pantothenate kinases (PANKs), the rate-limiting enzymes in CoA synthesis. Although CoA production is extensively...

The PLANT CYSTEINE OXIDASE (PCO) N-degron pathway contributes to oxygen sensing in plants. Here the authors identify HYPOXIA TRANSDUCER1 (HYT1) as a substrate of PCO and show it forms a coherent feed-...

The Lyme disease pathogen Borrelia burgdorferi contains a highly reduced genome lacking many primary metabolic pathways. However, B. burgdorferi retains the mevalonate pathway that synthesizes isopen...

Heme biosynthesis is tightly coordinated to support essential functions without accumulating toxic porphyrins and depleting cellular iron. Heme induces degradation of the heme biosynthetic enzyme, 5-a...

Abstract. Efficient purification of specific RNAs from lysates remains a major challenge. Existing methods, such as MS2 tagging or oligonucleotide hybridiz

The BqsRS two-component system regulates biofilms in the infectious pathogen Pseudomonas aeruginosa. Here, the authors show that the global response regulator BqsR controls Fe2+ acquisition in PAO1 th...

Deubiquitylases are key proteolytic regulators of ubiquitin-dependent cellular processes, catalyzing the removal or remodelling of ubiquitin modifications on substrate proteins, including those target...

ALT: Man Causes Own Bike Fall Meme

This publication highlight is part of the SBGrid Communities Project focused on science education and demonstrating how structural biology…

A methodology for mining protein structure databases on the basis of the distinct intrinsic structures of metal-binding active sites in enzymes enables the discovery of new families of radical halogen...

Liu et al. define how the CRL2ZYG11B ubiquitin ligase assembles and recognizes Gly/N-degron substrates by cryo-EM. Structures with an NLRP1 Gly/N-degron and SARS-CoV-2 ORF10 reveal extended substrate-...

Sweeping proposed rule, now open for comments, would also restrict foreign collaborations and remove federal funding for open-access fees

The Broad Institute Imaging Platform (the team behind CellProfiler, Piximi, and Bilayers) is currently hiring for postdoctoral and intern roles! Postdoc role: we are looking for 1-2 candidates who ha...

Heme, an iron-containing cofactor, is synthesized in mitochondria by an eight-enzyme pathway. Although cells were thought to manage heme autonomously, more than 1000 proteins contribute to its product...

The Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...