The prion concept postulates that seeded aggregation results in the recruitment of soluble protein monomers and their assembly into amyloid filaments with the same structures as those of the seeds. He...

In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly p...

The ciliary transition zone (TZ) regulates ciliary proteome composition, yet its molecular architecture, protein content, and contribution to motile ciliopathies remain poorly defined. We applied in situ cryo-electron tomography and subtomogram averaging ...

MemBrain v2 enables streamlined analysis of membranes in cryo-electron tomography by providing user-friendly tools for membrane segmentation, membrane protein localization and spatial analysis, suppor...

Mosquito-borne diseases are breaking records as climate change and other human influences allow the insects to expand their range.

Cryo-electron tomography uncovers the structure of a conserved hexameric ‘portal complex’ that spans the core wall in vaccinia virus, and shows how it is associated with viral functions such as a...

Multi-color super-resolution microscopy provides an atlas of SARS-CoV-2 viral RNA and proteins in infected human cells, uncovering nanoscale aspects of protease-dependent replication organelle maturat...

Family of rodent-borne viruses that can cause severe illness in humans

A quality and robustness release on top of 2.0.0b1, focused on the density-profile bilayer fitting (mesh refinement and thickness measurement), a forgiving centralized config system, and packaging ...

SerialEM is a widely-used program that supports several kinds of automated acquisition from transmission electron microscopes: tilt series, single particle data,…

ChimeraX Volume Operations Simplified Edition . Contribute to jparmachelab/ChimeraX-VOSE development by creating an account on GitHub.

In Mueller et al. the authors capture and visualize the native enzyme-substrate (Michaelis) and product complexes of nucleotide addition by bacterial RNA polymerase. Substrate coordination in a transition-state-like conformation, together with highly coordinated water molecules, suggests a positional (entropic) catalytic mechanism involving specific acid-base chemistry.

Henipavirus nucleoprotein assemblies exist in different conformations, indicating temporary release of genome for RNA synthesis.

Cas12a2 enables RNA-triggered, sequence-specific killing of eukaryotic cells via widespread DNA shredding, allowing selective elimination of cells on the basis of gene expression, including virus-infe...