iiinova — where your digital self keeps evolving

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Erik A. Rodriguez @erin-rod.bsky.social · 02/10/2026

🎃Behold FrankenPumpkin!🎃 I filled a flat bottom flask with chlorophyll extracted from algae.🦠UV light produced the red fluorescence. Jordan Tanen drew the pumpkin face.🖊️ #FluorescenceFriday #SciArt #SciComm #RealTimeChem #ChemArt

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Anh Hoang Le, PhD 🏳️‍🌈🏴󠁧󠁢󠁳󠁣󠁴󠁿🇻🇳 @anhhle2702.bsky.social · 02/10/2026

Happy #FluorescenceFriday. Single immune cell migration is fascinating. But these cells can also behave like a coordinated collective, like 🐝 from a hive. I captured some embryonic myeloid cells (🔴) displaying swarming behaviour in a migrating ectoderm cell sheet (🔵), like neutrophils in our body!

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James Manton @jamesdmanton.bsky.social · 29/09/2026

I've been working on a fast, high-ratio image compressor to try and alleviate the data storage issues myself and some of my colleagues have recently been facing. Before I 'release' version 1, it'd be great to gain some feedback regarding performance and missing features. github.com/jdmanton/rad...

github.com

GitHub - jdmanton/radelta

Contribute to jdmanton/radelta development by creating an account on GitHub.

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Anh Hoang Le, PhD 🏳️‍🌈🏴󠁧󠁢󠁳󠁣󠁴󠁿🇻🇳 @anhhle2702.bsky.social · 25/09/2026

Happy #FluorescenceFriday. The first time I imaged a 🐸 embryo, I didn't realise they had these ciliated cells on the outer surface. So you can imagine how surprised and curious I was! These cilia are similar to those found in the human airway. And yes, this is just a good old confocal + 🔥LUT!

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Heather Brown-Harding @microscopyed.bsky.social · 23/09/2026

Anyone have any seen red autofluorescence in the agarose pad while imaging c. elegans

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chrometra.com @chrometra.com · 08/09/2026

Our actin probes functionalized for expansion microscopy used in the MAGNIFY protocol. Cells: HeLa Magnification: 25x

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Journal of Cell Science @jcellsci.bsky.social · 11/08/2026

Our 'Cell Science at a Glance' articles are short primers that act as an introduction to an area of cell biology. Perfect if you're new to a field, or want to find out the latest on a particular topic. Check out the full list at: tinyurl.com/4c8e8m97 #cellbiology #community #notforprofit

A collage showing a selection of recent Cell Science at a Glance posters

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Harmit Singh Malik @harmitmalik.bsky.social · 09/08/2026

I’ve often said (partly tongue in cheek) that spite can be a great motivator. But mudita (freudenfeude), the ability to be truly happy for others’ success, can be a much more reliable way to have a fulfilling career. Even if it can seem that way, science is not a zero sum game. Play the long game.

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NeuroWoodworks @neurowoodworks.bsky.social · 09/08/2026

Mitochondrion on a block of walnut. I went all out on the sanding to make it super smooth on this one.

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George Campbell @geobellward.bsky.social · 06/08/2026

A small joy is to find a paper from the 80’s explaining something that seems to have somehow been forgotten.

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Nicolas Denans @nicolasdenans.bsky.social · 02/08/2026

any recommendation for a high throughput imaging system to sort zebrafish larvae based on fluorescence? Thinking about the VAST or AcquiferIM or..... Any input welcome!! #zebrafish

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Dr. Solomon David @solomonrdavid.bsky.social · 31/07/2026

Believe it or not we have addressed this! Here you go: single gar = gar multiple gars, same species = garrison multiple gars, different species = garmada

Graphic showing the different terminology for a single gar, a group of gar of the same species, and group of gar of multiple species. They are called the following, respectively: gar, garrison, garmada.

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St. Jude Children's Research Hospital @stjuderesearch.bsky.social · 15/07/2026

St. Jude scientists identified sub-compartments in the nucleolus that help lock ribosome building blocks in place. These findings highlight the role played by phase separation, shedding light on diseases linked to increased ribosome production. bit.ly/4bK3SzL

stjude.org

Research explains how nucleolus sub-compartments drive ribosome assembly

See how RNA and SURF6 sub-compartments surrounded by NPM1 help in ribosome biogenesis within the nucleolus granular component condensate

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Sarah McAnulty, Ph.D. @sarahmackattack.bsky.social · 07/07/2026

Alright I gotta go run a potentially soggy moth night Do me a kindness and close some states while I'm out talking urban biodiversity with my fellow Philadelphians 🥺 $10 to fill a bubble. Fill this map in: givebutter.com/USS2026

givebutter.com

United States of Science 2026

By Skype a Scientist

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George Campbell @geobellward.bsky.social · 16/06/2026

I feel like I am echoing the editorial, but the Points of View and Points of Significance series were very influential to my scientific journey. I am thrilled to see Points of View come back, being helmed by @helenajambor.bsky.social who has already done great work amongst the microscopy community

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Total Internal Reflection🇬🇧🇺🇸🇺🇦 @tirscienceblog.bsky.social · 07/06/2026

The #ThermoFisher #antibody scandal is an ugly reminder, I think, of how few people (including those at TF, it seems) are trained to properly validate antibodies before use. Some advice on antibody validation in the posting 👇 totalinternalreflectionblog.com/2022/09/18/h...

totalinternalreflectionblog.com

Hitting the target (a short guide to validating antibody specificity)

Antibodies are essential and ubiquitous tools in biology, but their unguarded usage can easily lead to error. Here’s how to validate their specificity, and ensure that they’re hitting the rig…

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Reese Richardson @reeserichardson.bsky.social · 05/06/2026

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

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Sebastian S. Cocioba @atinygreencell.bsky.social · 01/06/2026

Run controls. Always. Anything short of that isn't science. Full stop.

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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 01/06/2026

Update on GeneFrame alternative for bacterial imaging. After discussion with us and others, SunJin lab have how launched a substitute for timelapse imaging in geneframes www.sunjinlab.com/product/ispa... Dimensions are very similar, price is reasonable ($105 for 250) #microsky

sunjinlab.com

iSpacer 0.3mm – SUNJIN LAB

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Laurel Oldach @laureloldach.bsky.social · 28/05/2026

Thermo Fisher has responded to research integrity activists who spotted apparent manipulation in its antibody catalog: "In ... preparing antibody images for publication on our website, some images may have been adjusted to clarify for presentation purposes." More: cen.acs.org/research-int...

Headline "Sleuths say Thermo Fisher doctored data to sell antibodies," subhead "Apparent manipulation of validation images in reagent catalog concern researchers," byline Laurel Oldach, image of a pipette loading an SDS-PAGE gel for a western blot

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Mark Peifer (He, him) @peiferlabunc.bsky.social · 29/05/2026

translated: we used faked results to try and convince you to buy our over-priced antibodies, and then offer a set of lies to justify it

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Heather Brown-Harding @microscopyed.bsky.social · 21/05/2026

Check out my "rough draft" of my instrument status page for the HCBI. Any feedback before I set it up on an HCBI GitHub? hbrown227.github.io/lab-instrume...

hbrown227.github.io

Lab Instrument Status — Harvard

Live status of lab instruments at Harvard. Check availability before your session.

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Joachim Goedhart @joachimgoedhart.bsky.social · 19/05/2026

Combining biosensor imaging data with an animated plot of the quantified intensity, based on a blog I wrote earlier: thenode.biologists.com/crafting-plo... In this version I used a dark theme

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Anthony Dornan @dornan.bsky.social · 08/05/2026

#BlueSci question. Any recommendations for an (anti-rat) NIR 2y Ab to be used with a 730 nm laser line? The 2 obvious choices are AF750 or DyeLight755, with (on paper) AF750 brighter? Not sure about comparative photostability? No appropriate JaneliaFluors? @christlet.bsky.social any thoughts?

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Heather Brown-Harding @microscopyed.bsky.social · 22/04/2026

I think it would be cool to link up all the Imaging/Analysis YouTube Channels in the "Featured Channels" section of HCBI's YouTube homepage. What are some of the best we could highlight?

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Joachim Goedhart @joachimgoedhart.bsky.social · 10/04/2026

Some confetti to celebrate #FluorescenceFriday

Bacterial colonies expressing tagBFP / SGFP2 / mKOk and illuminated with UV

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Joyce Meiring @joycemeiri.bsky.social · 19/03/2026

What is the public opinion on color coding treatments in figures and then using the same colors used in the graph to outline matching representative images? Is it useful or am I being too extra?

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Moe Mahjoub @mahjoublab.bsky.social · 13/03/2026

Happy #FluorescenceFriday all! Checkout these beautiful basal bodies in airway #multiciliated cells expanded via U-ExM 😍 Images captured by the super talented @maneesha07.bsky.social on a #Nikon NSPARC 🔬.

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Heather Brown-Harding @microscopyed.bsky.social · 09/03/2026

Anyone (in the US) have strong opinions on what companies do the best fabric posters?

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BioImaging North America @bioimagingna.bsky.social · 06/03/2026

#HappyFluorescenceFriday #microscopycommunity - into Expansion Microscopy? Come to the Expansion Microscopy User Group Community Discussion Series! First session, Tue, March 10, on “Expansion Microscopy in Practice Series: Choosing the Right Protocol” Learn more: buff.ly/V4umk5E

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St. Jude Children's Research Hospital @stjuderesearch.bsky.social · 17/02/2026

St. Jude researchers and collaborators developed M-PACT, a tool that leverages AI to classify pediatric brain tumors using cell free DNA from cerebrospinal fluid. The approach could improve diagnosis and disease monitoring when tissue biopsy is not feasible. ow.ly/aUcU50Yh2bb

ow.ly

Classifying pediatric brain tumors by liquid biopsy using artificial intelligence

See how M-PACT, an AI-powered algorithm can sift through ctDNA in cerebrospinal fluid and molecularly classify tumors based on their DNA methylation pattern

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Joachim Goedhart @joachimgoedhart.bsky.social · 07/02/2026

Anyone ever tried super glue (cyanoacrylate) for mounting coverslips with stained mammalian cells, to be used for fluorescence microscopy? I'm looking for ways to conserve microscopy samples for a looooooong time...

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Emmanuel G. Reynaud @ereynaud.bsky.social · 15/01/2026

A loaded question to scream about...which agarose do you use to mix your beads to measure the PSF? Any brand #lsfm #lightsheet

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Jennifer Waters @jencwaters.bsky.social · 14/01/2026

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

meetings.cshl.edu

Quantitative Imaging: From Acquisition to Analysis

Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

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Joel Boerckel @jboerckel.bsky.social · 11/01/2026

Defending PhD student, looking over their thesis: “If I knew then what I know now, I could’ve done all of this in like 9 months.” A thread about my favorite pioneering cave explorers and why I don’t think AI will ever “solve” biology.

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Damian Dalle Nogare @damiandn.bsky.social · 09/01/2026

Want to up your microscopy and image analysis game? Applications open now for QI 2026! Join us in cold spring harbor for two weeks of intensive training in all things microscopy.

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Wendy Salmon 🐟🔬🧫 @fishcscells.bsky.social · 08/12/2025

Microscopy Bat signal! I want to compile a list of biological samples that demonstrate microscope capabilities, including the reagents to generate them. Please send me anything you know of!! Beads are great for QC, but there's a different teaching benefit with biology. 1/n

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Beth Cimini 🔬💻📊 @bethcimini.bsky.social · 13/11/2025

Just 4 days until the start of I2K and its 33 totally free image analysis tutorials and events! Please share with your "home networks", especially early career researchers - the videos will be amazing and high-impact no matter how many people attend live, BUT (1/x)

A fluorescent embryo on black text, with the words
Halfway to I2K: Virtual Tutorials on Image Analysis
November 17-19, 2025
Virtual Conference for beginners to developers
i2kconference.org
Image Credit - "Sweet Embryo",Travis D. Carney, BINA Image Contest 2024

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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 14/11/2025

Broader #bioimaging and #microsky people - anyone have any suggestions for sources of ~0.25mm non-cytotoxic double sided adhesive sheets?

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Alfred Millett-Sikking @amsikking.bsky.social · 12/11/2025

Has anyone had this problem? A hydrophobic water objective? The objective is great but we struggle to keep the water on the lens... -> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this? www.fishersci.com/shop/product...

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BioVoxxel (Jan Brocher) @biovoxxel.de · 10/11/2025

Ever wondered if analyzing your images by using intensity Z-projections impacts your data? Happy to announce my guest article in Microscopy & Analysis on that topic. It's a very short read but worth for everyone dealing with image analysis! buff.ly/KJ147tI #Microscopy #ZProjection #ImageAnalysis

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Kevin Terretaz @kwolbachia.bsky.social · 04/11/2025

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site #microscopy #ImageJ #FluorescenceFriday #microscopyMonday imagej.net/plugins/imag...

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Alex Merz 🇺🇸🇨🇦🇺🇦 @merz.bsky.social · 04/11/2025

One of the most useful resources for anyone who uses fluorescence methods.

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Rylie Walsh @ryliewalsh.bsky.social · 29/10/2025

Do you like building novel optical microscopes to answer complex biological questions? How about doing that as part of one of the world’s most unique and vibrant research and education communities, which is incidentally located in a beautiful seaside New England village? 🐙🌊🔬

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