iiinova — where your digital self keeps evolving

Reposted by Alfred Millett-Sikking

Loïc A. Royer 💻🔬🧪 @loicaroyer.bsky.social · 23/09/2026

What if any dataset, any size, any number of dimensions, opened in a browser tab from a link? 🔬🧪💻 Luxar is out today: write it in Python, share it as a link, explore it in any browser. Open source. 🧵 @biohub.org Preprint: doi.org/10.5281/zen... Code: github.com/royerlab/luxar

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Reposted by Alfred Millett-Sikking

FocalPlane @focalplane.bsky.social · 07/08/2026

Our #preprint list highlighting the latest tools and techniques in #microscopy is now up on FocalPlane. Let us know if you have any recommendations and/or give a shout out to your favourite(s)! focalplane.biologists.com/2026/08/07/m...

focalplane.biologists.com

Microscopy preprints – new tools and techniques in imaging - FocalPlane

Microscopy preprints – new tools and techniques in imaging - News

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Alfred Millett-Sikking @amsikking.bsky.social · 20/07/2026

Ultrafast 3D imaging just got faster! Introducing: "Instant dual-view Snouty (iDV-Snouty) light-sheet microscopy" -> Double the data rate of a Snouty microscope by adding a 2nd view! Use the extra view for a variety of imaging modes! Article here: amsikking.github.io/idv-snouty_l...

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James Manton @jamesdmanton.bsky.social · 02/06/2026

We present: Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution www.biorxiv.org/content/10.6...

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Ali Shaib @alishaib.bsky.social · 01/06/2026

Hello #world, meet 1,000× Expansion Microscopy. A small gel would grow to the size of an Olympic swimming pool, while amino-acid-scale distances become visible with ordinary light microscopy. Led by Helena Hu from @eboyden3.bsky.social's lab, in collab with us. Story: www.biorxiv.org/content/10.6...

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Torsten Wittmann @handle.invalid · 22/05/2026

A day in the life of a growing hiPSC spheroid expressing endogenously mStayGold-tagged histone 3.3 for #FluorescenceFriday and the #Snouty aficionados. 28 hours (and >50,000 exposures) later and they are still going! Home-brewed volume projection.

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Reposted by Alfred Millett-Sikking

BioImaging North America @bioimagingna.bsky.social · 18/05/2026

#HappyMicroscopyMonday #microscopycommunity - Join the BINA Builders for a panel and discussion on the shift from proprietary systems to community-driven innovation. Thursday, May 21! Learn more: buff.ly/pMShl9t

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Reposted by Alfred Millett-Sikking

Kevin Dean @kevin-dean.bsky.social · 12/05/2026

Altair Direct View OPM Preprint! We built a simplified direct-view oblique-plane microscope for imaging big, optically accessible specimens with a single-objective light-sheet geometry. Delivers... • ~5.4 mm field of view • ~2.6 μm, ~3.4 μm, and ~17.8 μm resolution in X/Y/Z

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Reposted by Alfred Millett-Sikking

Julia Lazzari-Dean @jlazzaridean.bsky.social · 12/05/2026

We invented “triplet tumbling microscopy” (TTM) to track protein-protein interactions in living cells! Tag your protein with GFP and see it interact with *untagged* binding partners www.biorxiv.org/content/10.6...

A cartoon overview of triplet tumbling microscopy, comparing data from bound and unbound samples.

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Reposted by Alfred Millett-Sikking

Julia Lazzari-Dean @jlazzaridean.bsky.social · 05/05/2026

We’re hiring a scientist to work on microscopy at Calico! If you’re excited about developing and applying cutting edge microscopy to a variety of biological questions, we’d love to hear from you. Find more information and apply here: www.calicolabs.com/careers/?gh_...

calicolabs.com

Careers - Calico

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Reposted by Alfred Millett-Sikking

Evelyn Strickland @strickland-evelyn.bsky.social · 20/04/2026

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

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Alfred Millett-Sikking @amsikking.bsky.social · 15/04/2026

Snouty objectives enable high resolution light-sheet microscopy with standard microscope platforms and sample formats! -> Here are citable repositories with CAD, Zemax and summarized optomechanical specifications: AMS_AGY_v3: a.k.a. Empress Snout, now the best! github.com/amsikking/AM...

github.com

GitHub - amsikking/AMS_AGY_v3_specifications: Optical and mechanical specifications for the AMS-AGY v3 objective (a.k.a Empress Snout).

Optical and mechanical specifications for the AMS-AGY v3 objective (a.k.a Empress Snout). - amsikking/AMS_AGY_v3_specifications

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Reposted by Alfred Millett-Sikking

Tanner Fadero @tanner-fadero.bsky.social · 10/03/2026

@patrickzager.bsky.social acquired this lovely 3D timelapse of live neutrophil membrane protrusions on the snoutscope at the UCSF Center for Advanced Light Microscopy. Shown are three orthogonal maximum intensity projections.

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Reposted by Alfred Millett-Sikking

Julia Lazzari-Dean @jlazzaridean.bsky.social · 09/03/2026

Hi microscopy folks! I'm looking for your advice 💡 We're shopping for cyclic immunofluorescence instruments to increase throughput and multiplex-ability of tissue IF. Have you worked with cyclic IF platforms (Comet, CellScape, Cell DIVE)? Thoughts? Pain points? Alternatives to consider? Thanks!

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Alfred Millett-Sikking @amsikking.bsky.social · 15/02/2026

The power of CAD! The ability to precisely draw 3D objects and have them made is still a miracle to me! -> Here's an old design for an alloy block to hold microcentrifuge tubes near freezing when moving them around. It's simple, but I wouldn't want to draw it or make it by hand...

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Alfred Millett-Sikking @amsikking.bsky.social · 15/02/2026

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "Z" scientist! (26/26) -> "Z" is for Zebra, Zucchini, and...Zernike? en.wikipedia.org/wiki/Frits_Z... Art by @annesikking.bsky.social (my Mum).

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Alfred Millett-Sikking @amsikking.bsky.social · 01/02/2026

Proof of principle. A critical step in many projects... -> Here's the first data from an AMS-AGY objective. Without building the whole instrument, it showed that a tilted Snouty (Frustum) objective can avoid the resolution lost in an oblique plane microscope! amsikking.github.io/high_na_sing...

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Alfred Millett-Sikking @amsikking.bsky.social · 01/02/2026

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "Y" scientist! (25/26) -> "Y" is for Yak, Yam, and...Young? en.wikipedia.org/wiki/Thomas_... Art by @annesikking.bsky.social (my Mum).

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Reposted by Alfred Millett-Sikking

Neuronal Signalling Lab @mjc-lab.bsky.social · 19/01/2026

Our snouty v3 (AMS-AGY v3) objective arrived today! Looking forward to getting it installed on our single objective light sheet system very soon...

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Alfred Millett-Sikking @amsikking.bsky.social · 18/01/2026

How has AI changed CAD? Can AI now reliably generate 3D parts, assemblies and drawings? I'm interested to hear about new tools, workflows and experiences ;) -> Here's a series of enclosures I designed years ago for housing custom lab equipment. Can AI CAD just generate this automatically now?

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Alfred Millett-Sikking @amsikking.bsky.social · 18/01/2026

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "X" scientist! (24/26) -> "X" is for Xeme, Xigua, and...Xanthopoulos? en.wikipedia.org/wiki/Basilis... Art by @annesikking.bsky.social (my Mum).

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Alfred Millett-Sikking @amsikking.bsky.social · 05/01/2026

Sharing: A simple automatic liquid dispense arrangement (SALDA) for timelapse microscopy. -> A SALDA enables accurate and repeatable liquid perturbations during timelapse microscopy and avoids environmental disturbance and vibrations from the user! Details here: amsikking.github.io/SALDA_for_ti...

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Alfred Millett-Sikking @amsikking.bsky.social · 05/01/2026

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "W" scientist! (23/26) -> "W" is for Worm, Watercress, and...Watt? en.wikipedia.org/wiki/James_W... Art by @annesikking.bsky.social (my Mum).

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Alfred Millett-Sikking @amsikking.bsky.social · 19/12/2025

Pro tip! As much as I like the 55deg #Snouty microscope configuration (previous post) it's hard to align and keep aligned! -> Consider using pre-designed O2-O3 optomechanics and an eyepiece to help avoid collisions during the set up! github.com/amsikking/SO...

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Alfred Millett-Sikking @amsikking.bsky.social · 19/12/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "V" scientist! (22/26) -> "V" is for Viper, Vanilla, and...Volta? en.wikipedia.org/wiki/Alessan... Art by @annesikking.bsky.social (my Mum).

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Reposted by Alfred Millett-Sikking

Julia Lazzari-Dean @jlazzaridean.bsky.social · 18/12/2025

I'm excited to be hiring an intern to develop new fluorescence-based assays for alpha synuclein aggregation at Calico during summer 2026 😃 🔬 If you're a current PhD student who loves microscopy, unnatural amino acids, and/or synuclein biophysics, please apply! www.calicolabs.com/careers/?gh_...

calicolabs.com

Careers - Calico

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Reposted by Alfred Millett-Sikking

Jon Daniels @jsdaniel02.bsky.social · 09/12/2025

The ASI machine shop made a nice replica model of the AMS-AGY v3 objective for us to have in the #CellBio2025 booth. The first of the real ones will be ready by the end of the year.

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Alfred Millett-Sikking @amsikking.bsky.social · 07/12/2025

Hands down my favorite #Snouty microscope configuration! -> The air objective with a 55deg light-sheet. It's not the highest resolution version, but the convenience of the air objective, the large field of view and the steep sectioning of the light-sheet make it a pleasure to use! #AIRR #miOPM

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Alfred Millett-Sikking @amsikking.bsky.social · 07/12/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "U" scientist! (21/26) -> "U" is for Unicorn, Ugli, and...Umov? en.wikipedia.org/wiki/Nikolay... Art by @annesikking.bsky.social (my Mum).

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Alfred Millett-Sikking @amsikking.bsky.social · 24/11/2025

Fast, gentle, 3D and high resolution makes miOPM ideal for timelapse microscopy of complex neural networks! -> Here's a unique iNeuron-iAstrocyte coculture imaged by github.com/gav-sturm and carefully prepared by @kunalshroff.bsky.social from the @kampmann.bsky.social lab. doi.org/10.1101/2025...

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Alfred Millett-Sikking @amsikking.bsky.social · 23/11/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "T" scientist! (20/26) -> "T" is for Tarantula, Turnip, and...Taylor? en.wikipedia.org/wiki/Brook_T... Art by @annesikking.bsky.social (my Mum).

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Alfred Millett-Sikking @amsikking.bsky.social · 12/11/2025

Has anyone had this problem? A hydrophobic water objective? The objective is great but we struggle to keep the water on the lens... -> Maybe a solution is to paint a circle around the nose with a 'hydrophobic barrier pap pen" (link below). Has anyone tried this? www.fishersci.com/shop/product...

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Alfred Millett-Sikking @amsikking.bsky.social · 09/11/2025

Another huge advantage of miOPM is that it's gentle! A light-sheet microscope that's compatible with standard slides and multi-well plates! -> Here we show 3D mitochondrial membrane potential in mammalian cells (GFP/RFP for mito, Cy5 for DNA). The cells move around freely! doi.org/10.1101/2025...

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Alfred Millett-Sikking @amsikking.bsky.social · 09/11/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "S" scientist! (19/26) -> "S" is for Squirrel, Strawberry, and...Snell? en.wikipedia.org/wiki/Willebr... Art by @annesikking.bsky.social (my Mum).

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Alfred Millett-Sikking @amsikking.bsky.social · 26/10/2025

A huge advantage of miOPM is 3D imaging with an air objective (previous post), but for extra depth you can also use a water objective! -> Here's mouse alveolar organoids with Muc1 AT2 cells (green), TOM20 mitochondria (blue), RAGE AT1 cells (gray) and DAPI (magenta). doi.org/10.1101/2025...

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Alfred Millett-Sikking @amsikking.bsky.social · 26/10/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "R" scientist! (18/26) -> "R" is for Rabbit, Radish, and...Raman? en.wikipedia.org/wiki/C._V._R... Art by @annesikking.bsky.social (my Mum).

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Alfred Millett-Sikking @amsikking.bsky.social · 13/10/2025

The future of High-Content Imaging has arrived. -> Ultra-fast 3D imaging of multi-well plates at high resolution using an air objective! See previous posts and the new miOPM preprint for details: doi.org/10.1101/2025...

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Alfred Millett-Sikking @amsikking.bsky.social · 13/10/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "Q" scientist! (17/26) -> "Q" is for Quail, Quince, and...Quinn? en.wikipedia.org/wiki/Helen_Q... Art by @annesikking.bsky.social (my Mum).

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Reposted by Alfred Millett-Sikking

Torsten Wittmann @handle.invalid · 08/10/2025

Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.

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Reposted by Alfred Millett-Sikking

Reto Fiolka @retof.bsky.social · 06/10/2025

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues. www.biorxiv.org/content/10.1...

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Alfred Millett-Sikking @amsikking.bsky.social · 28/09/2025

The culmination of many years work. One of the most advanced light-sheet microscopes in the world! -> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections! For details see previous posts and here: github.com/amsikking/HT...

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Alfred Millett-Sikking @amsikking.bsky.social · 28/09/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "P" scientist! (16/26) -> "P" is for Pelican, Pineapple, and...Planck? en.wikipedia.org/wiki/Max_Pla... Art by @annesikking.bsky.social (my Mum).

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Reposted by Alfred Millett-Sikking

Kari Herrington @kahsage.bsky.social · 18/09/2025

@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.

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Alfred Millett-Sikking @amsikking.bsky.social · 15/09/2025

What if we could get good 3D imaging in 'any' sample refractive index? Previously we showed how remote refocus optics can get good 3D imaging in a 'watery' sample (RI=1.33) with any immersion objective. -> Here we show how a zoom lens can adjust remote refocus optics to any sample refractive index!

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Alfred Millett-Sikking @amsikking.bsky.social · 14/09/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "O" scientist! (15/26) -> "O" is for Octopus, Orange, and...Ohm? en.wikipedia.org/wiki/Georg_Ohm Art by @annesikking.bsky.social (my Mum).

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Kevin Dean @kevin-dean.bsky.social · 02/09/2025

Thrilled to share our new work in Cell Reports Methods: MCT-ASLM, a multiscale light-sheet microscopy platform that captures biology from tissue landscapes down to organelles, revealing rare events like sparse neurons and micrometastases. www.cell.com/cell-reports...

cell.com

Feature-driven whole-tissue imaging with subcellular resolution

Lin et al. present a self-driving light-sheet microscope that integrates whole-organ surveys with automated, high-resolution targeting of either user-specified or autonomously identified features. By ...

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Alfred Millett-Sikking @amsikking.bsky.social · 01/09/2025

What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post). -> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!

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Alfred Millett-Sikking @amsikking.bsky.social · 01/09/2025

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "N" scientist! (14/26) -> "N" is for Narwhal, Nasturtium, and...Newton? en.wikipedia.org/wiki/Isaac_N... Art by @annesikking.bsky.social (my Mum).

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Torsten Wittmann @handle.invalid · 29/08/2025

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

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