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Shamphavi Sivabalasarma

@sshamphavi.bsky.social
517 followers 1.3K following 28 posts

Postdoc in Navarro lab, DMF-UNIL,Switzerland Jumping between bacterial and archaeal domains with a microscope ❄️🔬#teamtomo

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Reposted by Shamphavi Sivabalasarma
Svetlana Dodonova @dodonova-sveta.bsky.social · 13h
New preprint from Dodonova lab 🔔! Capturing multiple states by cryo-EM❄️, we show for the first time how archaeal RNA Polymerase engages a nucleosome and forms direct contacts with histones! 🧬 Great work by Gabriele & a fantastic collaboration with Dina Grohmann! 🧵 www.biorxiv.org/content/10.6...
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Simonetta Gribaldo @sgribaldo.bsky.social · 23/09/2026
Interested in #archaea, #methane, #cell-envelopes? New paper out @nature.com ! We discovered an enzyme that specifically cleaves the cell wall of methanogens, revealing a new chemical structure of archaeal peptidoglycan, 50 yrs after its first description www.nature.com/articles/s41... #MicroSky 🧵👇
nature.com
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 23/09/2026
What a paper Simonetta! Congrats 🎉
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Reposted by Shamphavi Sivabalasarma
Florent Waltz @florentwaltz.bsky.social · 22/09/2026
... For the sponsoring the talk and poster prizes, that went to @fannyleblanc.bsky.social and Aurélien Gregor 🎊. And huge thanks to @sofie-dot-rec.bsky.social, @phaips.vd.st and Imre Gonda for teaching the workshop on sample prep, data collection and processing on the 2nd day. #cryoET #TeamTOMO
Poster prize to Fanny Le Blanc
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Reposted by Shamphavi Sivabalasarma
bioRxiv Microbiology @biorxiv-microbiol.bsky.social · 16/09/2026
Ribosome profiling reveals translational dynamics between an archaeal virus and its host www.biorxiv.org/content/10.64898/20…
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Reposted by Shamphavi Sivabalasarma
Cameron Thrash @jcamthrash.bsky.social · 08/09/2026
Molecular basis of N2 fixation in a hyperthermophilic archaeon www.nature.com/articles/s41... #jcampubs
nature.com
Molecular basis of N2 fixation in a hyperthermophilic archaeon - Nature Communications
Nitrogenases are enzymes that convert atmospheric N2 into ammonia, thus providing bioavailable nitrogen to living organisms. Here, Maslać et al. describe a highly thermostable, simple nitrogenase from...
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Reposted by Shamphavi Sivabalasarma
Ricardo D. Righetto @lifeonthewedge.bsky.social · 08/09/2026
A wild publishing ride to get here... but finally! (first bioRxiv: January 5th, 2024 🙃) Amazing work from @lorenzlamm.bsky.social and colleagues 🤗 Special thanks to the community who jumped early on the boat, sharing feedback and training data to make MemBrain v2 a reality 🙏🏽
nature.com
MemBrain v2: an end-to-end tool for the analysis of membranes in cryo-electron tomography - Nature Methods
MemBrain v2 enables streamlined analysis of membranes in cryo-electron tomography by providing user-friendly tools for membrane segmentation, membrane protein localization and spatial analysis, suppor...
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 05/09/2026
Congrats!!🎉
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Reposted by Shamphavi Sivabalasarma
EMBL @embl.org · 03/09/2026
Congratulations to @florianwollweber.bsky.social, the recently appointed Group Leader at EMBL Grenoble, who received an ERC Starting Grant worth approximately €2 million to investigate the origins of eukaryotic cell complexity. bit.ly/4gNwEBk
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Reposted by Shamphavi Sivabalasarma
Alex Merz 🇺🇸🇨🇦🇺🇦 @merz.bsky.social · 31/08/2026
Our new paper with @ckellison.bsky.social. is finally out! We identify an ancient and widely conserved mechanism that promotes initiation of fiber polymerization in type IV pili and type II secretion systems.
pnas.org
β-strand addition within tip initiation complexes licenses assembly of diverse type IV filaments | PNAS
PilC/PilY1 proteins are tip-located adhesins of type IV pili (T4P) that are critical for T4P function in diverse behaviors including twitching moti...
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Reposted by Shamphavi Sivabalasarma
Tanmay Bharat @tbharat-lab.bsky.social · 23/08/2026
Molecular architecture of colossal surface layers from hyperthermophilic archaea Led by @idocaspy.bsky.social (Ido) In collaboration with @mkrupovic.bsky.social and @vikramalva.bsky.social labs doi.org/10.64898/202...
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Julia Meltzer @juliameltzer.bsky.social · 13/08/2026
Excited to share what I've been working on with an incredible team! Visualisation of Asgard viruses on cell surfaces and vesicles, and a story of complex viral interactions. Preprint: www.biorxiv.org/content/10.6... @brendanburns999.bsky.social @belindaferrari.bsky.social @xabivc.bsky.social
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 06/08/2026
Hot, acidic and an endlessly fascinating archaeal model organism! Check out our review on Sulfolobus acidocaldarius🥳
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Reposted by Shamphavi Sivabalasarma
Pedro Leão @pedroleao.bsky.social · 27/07/2026
The manuscript introducing ArchaeaHQ is still under review😴 As science can't wait... I'm making available a version of the dashboard we use. vee-lab.eu/archaeahq Here the user can have quick access to the information in the database, and select specific genomes of interest. Enjoy 🎉
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Evgenii Protasov @evgenii-protasov.bsky.social · 27/07/2026
Two types of sheathed archaellum structures from Haloarcula marismortui differ in their outer layer architectures #microbiology #archaea #MicroSky #ArchaeaSky @natcomms.nature.com www.nature.com/articles/s41...
nature.com
Two types of sheathed archaellum structures from Haloarcula marismortui differ in their outer layer architectures - Nature Communications
Archaea can swim using rotating helical filaments called archaella. Here, Meshcheryakov et al. report that the cryo-EM structures of two types of archaella from Haloarcula marismortui display a unique...
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Reposted by Shamphavi Sivabalasarma
Brett Baker @archaeal.bsky.social · 23/07/2026
www.science.org/doi/10.1126/...
science.org
Eukaryotic-like microtubules and dynamic instability of Asgard archaeal tubulins
Asgard archaeal mini microtubules suggest an archaeal origin of eukaryotic microtubules.
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bioRxiv Microbiology @biorxiv-microbiol.bsky.social · 16/07/2026
Systematic identification of cell division proteins in haloarchaea and the discovery of a membrane anchor for the Z ring www.biorxiv.org/content/10.64898/20…
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Gregor Weiss @weiss-lab.bsky.social · 10/07/2026
Some bacteria do not live as individuals. They build multicellular filaments and connect neighboring cells through tiny cell-cell junctions. Cyanobacteria are among the most beautiful examples 😍 In our new paper, we reveal first molecuar insights on the cyanobacterial septal junction architecture 🧵
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Paula Navarro @navarropaula.bsky.social · 03/07/2026
🚨 New in @natmicrobiol.nature.com! We reveal how the antibiotic target PBP1b fortifies the E.coli division site against osmotic rupture. Proud this was completed in our independent labs @dmf-unil.bsky.social, with @avettiger.bsky.social. Congratulations to all authors! 🦠❄️🔬 #teamtomo bit.ly/3SCbOg1 👇
doi.org
The penicillin-binding protein PBP1b fortifies the Escherichia coli division site against osmotic rupture - Nature Microbiology
A specific isoform of PBP1b functions independently of the activator protein, LpoB, to drive generation of a wedge-like peptidoglycan structure that strengthens the division site in Escherichia coli.
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Reposted by Shamphavi Sivabalasarma
Alex Crits-Christoph @acritschristoph.bsky.social · 23/06/2026
ohhhhh man it is another amazing paper from @masarunobu.bsky.social that's a Microgenomates growing on a Chloroflexi:
Fig. 2 | Parasitic interaction between strain OT8 and host strain Mc4.
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Faculty of Biology - University of Freiburg @biologyunifreiburg.bsky.social · 21/06/2026
Diese ausführliche und äußerst informative Dokumentation zeigt, welche Rolle Stickstoffdünger in der Landwirtschaft spielen und wie die Arbeit von Thomas Ott und seinem Team dazu beiträgt, die Lebensmittelproduktion nachhaltiger zu machen. Schauen Sie doch mal rein! 🌱🍅🧪
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 19/06/2026
Beautiful!!
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Svetlana Dodonova @dodonova-sveta.bsky.social · 19/06/2026
Excited to share our new preprint! 🧬❄️ by brilliant @mdreimann.bsky.social and great collaborators! Using cryo-ET&EM, we reveal archaeal chromatin in a near-native state: variable-beads-on-a-string fibers shaped by growth phase and histone composition #ArchaeaSky www.biorxiv.org/content/10.6...
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Evgenii Protasov @evgenii-protasov.bsky.social · 17/06/2026
A multilayered cell envelope of a member of the Chloroflexota offers an anchoring platform for the archaellum #microbiology #archaea #bacteria #MicroSky doi.org/10.3389/fmic...
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Reposted by Shamphavi Sivabalasarma
Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · 01/06/2026
🛠️💉It's time for engineering contractile injection systems! @chipericson.bsky.social & @davidschaumann.bsky.social introduce programmable CIS - PROCIS, combining tail length control, non-native cargo loading, and cell retargeting all in one system! www.biorxiv.org/content/10.6... 🧵 1/5
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BejaLabTechnion @bejalab.bsky.social · 26/05/2026
Active virus-host system in a Lokiarchaeon culture www.biorxiv.org/content/10.6...
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Reposted by Shamphavi Sivabalasarma
MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 26/05/2026
Led by Hannah Ochner, @tbharat-lab.bsky.social’s group have developed correlated #cryoEM & mass spectrometry imaging, capable of sub-cellular chemical mapping. With @kiranrpatil.bsky.social’s group, they have imaged the cellular uptake of forever chemicals. mrclmb.ac.uk/news-events/... #LMBResearch
Diagram showing cryo-FIB-SIMS technique
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Wei He (何炜) @weiwaer.bsky.social · 20/05/2026
My first story about Archaea cell division comes out !!! A three protein system (Dip system) positions H. volcanii divisome to midcell. Dip system works like bacterial Min sytem, but they share NO sequence homology "All roads lead to Rome😊".
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 20/05/2026
How do archaea find mid cell? They invent their own new system! Check out the preprint and admire those beautiful fluorescence images of @weiwaer.bsky.social Happy to have been part of it🎉
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bioRxiv Microbiology @biorxiv-microbiol.bsky.social · 20/05/2026
A conserved oscillatory system that positions the divisome in Archaea www.biorxiv.org/content/10.64898/20…
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Joshua Hamm @joshnhamm.bsky.social · 19/05/2026
Our paper examining the evolution of halophily in DPANN is out now in MBE! academic.oup.com/mbe/advance-...
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cryoEM papers @cryoempapers.bsky.social · 15/05/2026
Recent advances in integrative cryoEM/ET toward visualizing the molecular sociology of cellular organelles pubmed.ncbi.nlm.nih.gov/42134003/ #cryoEM
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Michael Wagner @michiwagner4.bsky.social · 10/05/2026
Cool discovery. Hydrogenobodies in rumen protozoa support methanogenesis #MicroSky 🧪 #ArchaeaSky www.science.org/doi/10.1126/...
science.org
Rumen ciliates modulate methane emissions in ruminants
Rumen ciliates are major contributors to enteric methane emissions from ruminant animals, yet the underlying mechanisms remain poorly understood. We present a catalog of 450 rumen ciliate genomes, wit...
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Archaea Biology Vienna @archaea-vienna.bsky.social · 11/05/2026
Do biofilms give an ecological advantage? Yes! A fantastic follow-up to our previous biofilm work in ammonia-oxidizing archaea: "Biofilm Lifestyle Drives Ecophysiological Niche Expansion in an Archaeal Soil Nitrifier" www.biorxiv.org/content/10.6... Congratulations to all authors! #archaea
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Evgenii Protasov @evgenii-protasov.bsky.social · 10/05/2026
Some archaea like coffee too 🤓☕️ Using coffee waste as feedstock for continuous cultivation of Sulfolobus acidocaldarius #microbiology #archaea #coffee #Sulfolobus #MicroSky #ArchaeaSky www.sciencedirect.com/science/arti...
sciencedirect.com
Using coffee waste as feedstock for continuous cultivation of Sulfolobus acidocaldarius
Sulfolobus acidocaldarius is a thermoacidophilic archaeon with high potential for robust continuous bioprocesses under extreme conditions. In this stu…
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Evgenii Protasov @evgenii-protasov.bsky.social · 04/05/2026
Do #archaea and #bacteria follow the same evolutionary "rules" when moving into a host🧐? Our latest study shows striking parallels in how intestinal and endosymbiotic archaea evolved compared to bacteria! #microbiology #methanogens @microbiomej.bsky.social link.springer.com/article/10.1...
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Sonja-Verena Albers @archaellum.bsky.social · 27/04/2026
Indeed, a lot of fun, and I would have not thought that I would publish anything about the bindosome ever again! 😁
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
This was incredibly fun figuring out with @archaellum.bsky.social and I am happy to revive the bindosome again likely found in more archaea than just Sulfolobales!
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
This suggests that TFF systems are not mere surface filaments but can be functionalized for substrate scavenging! And if anyone remembers just like bindosomes that @archaellum.bsky.social described once!
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
we screened for proteins with a class III signal peptide cleavage site, a hallmark for pilins. And surprise archaea encode for several substrate binding proteins with a class III signal peptidase site!
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
But what could their function be? (4/n)
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
We wondered what drove the variability in the systems. Using structure-guided phylogenetics, we saw that structural expansion of core components drove the diversification of the archaeal TFF superfamily! (3/n)
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
We find that archaea encode a striking diversity of TFF systems, up to 12 distinct systems per genome, exceeding the diversity typically observed in bacteria.
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
Back in 2016, Kira Makarova provided a first glimpse into the diversity of archaeal TFF systems. Since then, the archaeal domain has expanded substantially, with multiple newly described phyla. So we rescreened the genomes using MacSyFinder2 for various archaeal TFF systems (2/n)
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Shamphavi Sivabalasarma @sshamphavi.bsky.social · 27/04/2026
You ever wondered whether your favorite archaeon has a surface structure or even an archaellum? Say no more! We screened for the presence of archaeal TFF machineries in all archaeal phyla and help you with that! Check it out here: www.biorxiv.org/content/10.6... (1/n)
biorxiv.org
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BejaLabTechnion @bejalab.bsky.social · 20/04/2026
Bridging the membrane lipid divide: bacteria of the FCB group superphylum have the potential to synthesize archaeal ether lipids pmc.ncbi.nlm.nih.gov/articles/PMC...
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Martin Loose @nartimsoole.bsky.social · 16/04/2026
Now out in its final form! www.science.org/doi/10.1126/...
science.org
Science | AAAS
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Tomas Pascoa @tomaspascoa.bsky.social · 14/04/2026
1/ Excited to share our preprint! 🥳 Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine www.biorxiv.org/content/10.6...
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Anuj @rnfr2d2.bsky.social · 14/04/2026
How do “LEGO-like” electron-bifurcating modules combine to drive degradation in BTEX-contaminated ecosystems? Check out our latest preprint, where we use cryo-EM and cryo-ET to reveal how the 1 MDa BCRII complex powers extremely endergonic aromatic ring reduction. www.biorxiv.org/content/10.6...
biorxiv.org
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Debnath Ghosal @debnathghosal.bsky.social · 12/04/2026
One of biggest mysteries in biology: how did complex eukaryotic cells evolve from simple microbes? ~1.8 billion years ago, an archaeal cell likely merged with a bacterium to form the first eukaryotic cell, but can we ever find direct evidence of this transformative event? 🦠 🚶‍♂️
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