Saturn’s icy moon Enceladus features chemical signatures consistent with an alkaline soda ocean with H2 production via hydrothermal water-rock reactions. Chemolithoautotrophic methanogenesis is thermo...

Abstract. The archaeal symbiotic Nanobdellales have been isolated with their hosts from a shallow hot marine vent off Iceland and from terrestrial hot spri

Karst aquifers represent dynamic subsurface environments where hydrological fluctuations and redox variability can strongly influence groundwater microbiomes...

The integration of multiple meta-omics can tremendously improve our understanding of the structure, function and dynamics of the complex biological system in anaerobic digesters. Created in BioRender...

Anoxic live-cell imaging shows actin-dependent crawling motility and dynamic protrusions in Asgard archaea, providing insights into cellular innovations that may have contributed to eukaryotic evoluti...

Abstract. The increasing prevalence of antimicrobial resistance requires efficient strategies to prioritize biosynthetic gene clusters (BGCs) that may enco

Abstract. Parasitic fungi of the phylum Chytridiomycota are known parasites of phytoplankton yet little is known about drivers of their diversity and host

Oxytricha species are widely distributed in freshwater and terrestrial ecosystems, playing significant ecological roles in microbial communities. Their ability to undergo encystment provides a powerfu...

Anaerobic methanotrophic archaea (ANME) of the ANME-1 clade are pivotal regulators of the global carbon cycle, yet the genomic basis underlying their ecological diversification and broad environmental...

Plastic pollution creates new habitats for microbes in the ocean, but little is known about how it affects archaea. Here, the authors characterized the plastisphere archaeome and the associated potent...

Electroactive microbiomes couple direct, mediated, conductive, and interspecies electron transfer with biofilm organisation to drive wastewater treatment, bioremediation, resource recovery, carbon co...

Methanogenic archaea are key syntrophic partners in animal and human guts. However, the molecular basis of physical and functional interactions of methanogen...

The Rhizarian amoeba Paulinella chromatophora represents a unique case of primary endosymbiosis, which has acquired photosynthetic organelles (chromat…

Here, the authors surveyed the vertical microbiome in the Qinghai-Tibet Plateau and assessed the degradation patterns, biogeochemical cycling, and nutrient loss across these stratified microbiomes.

Methanogenic archaea harbor diverse enzymes that support their survival in extreme environments and play key roles in global carbon cycling. The C39 peptidas...

Environmental genomics has focused mainly on prokaryotes, leaving microbial eukaryote diversity poorly understood. Using ~20,000 mitochondrial genomes from 3,400 lake metagenomes worldwide, this study...

Norovirus is the leading global cause of acute gastroenteritis. Here, the authors elucidate norovirus replication and show that the viral NS3 protein forms an RNA-engaging molecular motor that may tra...

Communications Biology - Two coinfecting archaeal viruses provide insights into virus-virus interactions

Researchers reveal that comammox Nitrospira become dominant nitrifiers in alkaline thermokarst lakes on the Tibetan Plateau and uncover their ecological adaptations to extreme saline-alkaline environm...

Using bioinformatics, biochemistry, NMR, as well as the newly characterized enzyme ArmA from methanogenic archaea, we revised the existing knowledge on archaeal peptidoglycan structure. At the same ti...

Antimicrobial resistance (AMR) is one of the major global public health threats. In recent decades, Acinetobacter baumannii has become one of the most significant multidrug-resistant (MDR) bacteria in hospital settings (1, 2). Since 2017, the World Health Organization has classified carbapenem-resistant A. baumannii as a critical-priority pathogen, a status that remains in the most recent update in 2024, which reflects the urgent need for new antibiotics against this bacterium (3, 4). A. baumannii is a frequent cause of nosocomial infections, primarily affecting patients in intensive care units (ICUs), immunocompromised individuals, patients using medical devices (such as catheters), and those with underlying diseases (5).

In this Review, Reinke examines recent advances in microsporidia research, from their evolution, to host invasion, proliferation, transmission, host interaction, immune evasion, detection and treatmen...

Abstract. Widespread and chronic heavy metal pollution resulting from industrial activities has compromised the sustainability of soil ecosystems. Increasi

ArmA from Methanobrevibacter smithii, a dominant member of the human gut microbiome, is identified and characterized as a glycosyl hydrolase specific for cleaving the cell wall of methanogens, reveali...

SUMMARYRNA modifications in the tRNA, rRNA, and mRNA constitute a widespread layer of post-transcriptional gene regulation, commonly known as the epitranscriptome. In bacteria, tRNA and rRNA modificat...

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Cyanobacteria are the only prokaryotes to have evolved oxygenic photosynthesis, transforming the geochemistry and biology of the planet. They exhib...

A biofilm-like mode of growth is hypothesized to be required for the emergence of cellular life. Occurrence of common biofilm gene products in current Bacteria and Archaea is an indication for their ....

npj Biofilms and Microbiomes - Circadian regulation of the gut microbiome in ruminants: opportunities for targeted microbiota manipulation and optimized timing for interventions

Abstract. Anaerobic fermentation with microbial communities is an emerging platform for converting lignocellulosic biomass to biofuels and bioproducts. The

The rumen is a complex anaerobic fermentation ecosystem in which microbial communities undergo dynamic changes during host development, influencing fermentation efficiency, hydrogen utilization, and m...

The amoeba Incendiamoeba cascadensis demonstrates that eukaryotic life can withstand temperatures beyond what was thought possible and sheds light on molecular strategies for survival in extreme heat.

Abstract. Methane production by a biogas digester is a promising alternative to the use of fossil fuels as an energy source. One major substrate for methan

Abstract The genus Methylococcus is represented by obligately aerobic gram-negative cocci and diplococci, which utilize methane as a source of carbon and energy. These bacteria inhabit a wide spectrum...

A study reveals an independent case of plastid loss and a metabolic partnership with a bacterial endosymbiont in an understudied lineage of non-photosynthetic ochrophytes.

Abstract For the first time, this study describes a bacterial redox process of anaerobic degradation that simultaneously involves two key organic osmoprotectants: glycerol as an electron donor and bet...

Abstract Analysis of microbial community composition from five hot springs of the Uzon Caldera (Kamchatka, Russia) using molecular and classical microbiological methods revealed that these thermal eco...

Abstract. Characterizing CRISPR interference (CRISPRi) phenotypes presents a fundamental temporal challenge: pre-existing overabundance of target proteins

Snow cover, the extensive terrestrial habitat in Antarctica, sometimes exhibits vivid coloration, yet the structure and function of its microbial communities remain poorly characterized. Using metagen...

Hydrocarbons in the long-term hydrocarbon cycle (LTHC) are produced over long time scales, whereas cyanobacterial n-alkane production occurs on short time scales in the short-term hydrocarbon cycle (S...

Background Methane is a potent greenhouse gas with profound implications for climate change, and its global emissions are largely driven by biological methanogenesis—a process long thought to be restr...

Abstract. As Earth’s principal reservoir of organic carbon and microbial biomass, the deep subsurface hosts microorganisms capable of mobilizing this once-