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@protocolsio.bsky.social
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A secure platform for developing and sharing reproducible methods.

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Erin Hahn (she/her) @erinhahn.bsky.social · 16/09/2026
Join us next week for a chat about Reproducible methods using @protocolsio.bsky.social 🔬 Register today! 👇
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Stephanie Hampton @sehampton.bsky.social · 17/09/2026
Winter #limnology sampling is on the horizon! Our Winter Limnology Network team recently posted our official protocols for sampling under the ice in seasonally frozen lakes - check them out, use them, go get some winter data! www.protocols.io/view/limnolo...
Secchi disk on rope lowered through hole in the ice on a lake
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Northeastern University Library @northeasternlib.bsky.social · 11/09/2026
Get your questions about protocols.io answered during expert Mericka McCabe's office hours. 📆 Friday, September 18 ⏰ 9 - 10 a.m. EDT 📍 Snell Library 215 🔗 northeastern.libcal.com/event/17469987
northeastern.libcal.com
Meet with an expert: protocols.io
Northeastern researchers have Premium access to protocols.io, an experimental workflow tool and open methods repository that allows you to find, create, store, run, and share...
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www.protocols.io @protocolsio.bsky.social · 09/09/2026
Enabling Reproducible Science Methods locked in static documents don't scale. Join researchers from CSIRO, ANU and Waters Biosciences for a free session on making research workflows reproducible and collaborative. Register here: go.springernature.com/anz-webinar-...
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smahtnetwrk.bsky.social @smahtnetwrk.bsky.social · 28/08/2026
Explore a step-by-step NanoSeq library preparation protocol using mung bean nuclease for whole-genome duplex sequencing. Optimized across 15+ human tissues, the method enables improved genome coverage for high-sensitivity somatic mutation detection. www.protocols.io/view/optimiz...
protocols.io
Optimized Mung Bean Nuclease Nanoseq Library Preparation Protocol for Whole Genome Sequencing
Optimized Mung Bean Nuclease Nanoseq Library Preparation Protocol for Whole Genome Sequencing. Duplex. Read full protocol, steps, and materials on protocols.io
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Carl Zimmer @carlzimmer.com · 27/08/2026
The lab is a mysterious place--where some people have "magic hands," and some experiments just won't work. Here's my story about the unspoken knowledge of science, and the struggle to share it. Gift link: nyti.ms/3UccF7Z
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SPARC @sparcopen.bsky.social · 26/08/2026
Read our new impact story on Michael J. Fox Foundation & @asapresearch.parkinsonsroadmap.org. Updated #OpenScience requirements aim to accelerate discovery & improve treatment for Parkinson’s disease. It's an inspiration to other @orfg.bsky.social members. sparcopen.org/impact-story...
sparcopen.org
Parkinson’s Research Funders Lead the Way with Progressive Open Access and Open Science Policies - SPARC
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www.protocols.io @protocolsio.bsky.social · 21/08/2026
@springernature.com is sponsoring Lab of the Future Conference! Our protocols.io, Experimental Workflow Solutions and AI Growth & Partnerships teams will be there. Let’s connect on digital workflows and research ecosystems accelerating discovery. Join us there: www.lab-of-the-future.com/europe
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Max Farnworth @maxfarnworth.bsky.social · 12/08/2026
Our book chapter from 7 (?!?!) years ago, is now also available as protocols.io version! That's pretty neat. www.protocols.io/view/the-red...
protocols.io
The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tr...
The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development. . Read full protocol, s...
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Ignacio J. Melero-Jiménez @ignamelero.bsky.social · 21/07/2026
🧬 We’ve shared an open-access protocol for cost-effective, high-quality DNA extraction from cyanobacteria and microalgae. We hope it will be useful to the research community! Developed with @Raul_MontanezM. doi.org/10.17504/pro... #Cyanobacteria #Microalgae #DNAExtraction #Genomics #ProtocolsIO
doi.org
Lab protocol: DNA extraction from cyanobacteria and microalgae
Lab protocol: DNA extraction from cyanobacteria and microalgae. Photosynthetic microorganisms are key. Read full protocol, steps, and materials on protocols.io
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Emory CIDMATH @emorycidmath.bsky.social · 22/07/2026
Access the full protocol on protocols.io: www.protocols.io/view/rsv-a-a...
protocols.io
Bring structure to your research - protocols.io
A secure platform to develop, share, and discover reproducible research methods, protocols, and workflows across teams and the global scientific community.
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Emory CIDMATH @emorycidmath.bsky.social · 22/07/2026
"RSV-A and RSV-B Amplicon Sequencing from Solid-Phase Wastewater Using the NEBNext® RSV Primer Module" is a novel protocol by CIDMATH researchers Said Rachida, Alaa Ahmed, Hannah Cayla D., Abby Paulos, Orlando Sablon, Marlene Wolfe, and Anne Piantadosi.
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Ben Pascoe @benizao.bsky.social · 26/06/2026
Open science has been central to this project from the beginning. We've made our laboratory and sequencing protocols openly available so that others can build on the framework. 🧫 Laboratory protocols www.protocols.io/view/getcamp... 🧬 Sequencing protocols www.protocols.io/view/getcamp...
protocols.io
GetCampy_Laboratory Procedures V.1
GetCampy_Laboratory Procedures. This standard operating procedure (SOP) outlines the preparation, qual. Read full protocol, steps, and materials on protocols.io
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Ben Pascoe @benizao.bsky.social · 26/06/2026
🧵 New paper! 🚨 Delighted to see our GETCampy-Africa protocol published in BMJ Open. *Protocols for genomic epidemiology and source attribution of enteric bacteria causing diarrhoeal disease across Africa* @bmj.com @bmjmedicine.bsky.social 📄 bmjopen.bmj.com/content/16/6... 👇
bmjopen.bmj.com
Protocols for genomic epidemiology and source attribution of enteric bacteria causing diarrhoeal disease across Africa
Introduction Enteric bacterial pathogens are a major cause of diarrhoeal disease in low-income and middle-income countries, with complex transmission pathways involving human, animal and environmental...
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Tom Pennance @schistosoma.bsky.social · 27/05/2026
A protocol for performing whole genome sequencing of individual Schistosoma haematobium larva stored on FTA cards. Following this protocol (inc. diagnosis), with list price consumables, I currently estimate one can achieve a ~50x coverage of each genome for ~£104 www.protocols.io/view/schisto...
protocols.io
Schistosoma haematobium larva FTA-to-WGS
Schistosoma haematobium larva FTA-to-WGS. This protocol describes the isolation of individual schistos. Read full protocol, steps, and materials on protocols.io
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Zebrafish Rock! @zebrafishrock.bsky.social · 25/04/2026
As we near the end of #EarthWeek, a reminder that #zebrafish do not need sterile dishes! Save money and reduce plastic consumption by sanitizing used dishes with hot water or dilute bleach 🧫💦 For example, you could follow this protocol from the Fish Floor at UCL: www.protocols.io/view/washing... 🐟 🧪
Image of clean petri dishes drying on a towel. They are neatly sorted and very shiny! Credit to Prof. Cecilia Moens.
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Matt Lycas @lycasworks.bsky.social · 27/04/2026
www.protocols.io/view/cryo-ex... New protocol alert!! Cryo-expansion microscopy of C. elegans and Tardigrades using the high pressure freezer!
protocols.io
Cryo-Expansion Microscopy of C. elegans and Tardigrades
Cryo-Expansion Microscopy of C. elegans and Tardigrades. Expansion microscopy (ExM) improves imaging r. Read full protocol, steps, and materials on protocols.io
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Doug Dyer @douglaspdyer.bsky.social · 01/05/2026
delighted to share our new pre-print developing label-free glycosaminoglycan analysis of diverse complex biological samples, led by @h-davies-s.bsky.social working closely with David Knight and Rebecca Miller alongisde our recent recruit Samie Dalgarno www.biorxiv.org/content/10.6...
biorxiv.org
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www.protocols.io @protocolsio.bsky.social · 28/04/2026
Modern research is increasingly collaborative, creating a need for clearer, shared workflows. Join us on May 5, 2026 at 14:00 CET | 13:00 GMT for a webinar on how research teams are adapting and collaborating at scale. Register here: go.springernature.com/int1/pf040/e...
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www.protocols.io @protocolsio.bsky.social · 22/04/2026
We’re transforming research protocols from static documents into dynamic, reusable workflows. A key part is making large collections of trusted methods — including Protocol Exchange and Springer Protocols — available in protocols.io format, so researchers can reuse, adapt, and build on them.
protocols.io
Evolving protocols: From static to dynamic with protocols.io
Sharing research protocols is a cornerstone of open science, and protocols.io, part of Springer Nature, continues to evolve as a secure, modern platform for...
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Springer Nature @springernature.com · 29/03/2026
Strengthening transparency and reproducibility starts with sharing research work openly. Our latest blogs offer guidance for: > How to share research code openly: spklr.io/63329Eynzz > How to share research protocols and methods openly: spklr.io/63322EynzO
SPRINGER NATURE: Open Science | Step-by-step guides. Share your research code and protocols openly. Best practices for transparency and reuse. Read the blogs. Abstract blue and purple design element on right.
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www.protocols.io @protocolsio.bsky.social · 19/03/2026
Attending ABRF 2026 (@abrf.bsky.social)? Connect with protocols.io at Booth 710. We're engaging with core facilities about common priorities and challenges, including how to demonstrate impact and receive credit. If this resonates, schedule a short discussion with our team: abrf26.youcanbook.me
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Noura Maziak @nmaziak.bsky.social · 10/03/2026
Excited to share my PhD work! The question: How is genome organization established? Here, we developed a low-input Micro-C method, Pico-C, and used it to map chromatin architecture across early stages of fly development. Here’s a quick Blue-torial 🙂 (1/n) www.nature.com/articles/s41...
nature.com
Three-dimensional genome reorganization foreshadows zygotic genome activation in Drosophila - Nature Genetics
Pico-C, a low-input Micro-C approach, reveals that dynamic three-dimensional genome folding precedes zygotic genome activation in Drosophila.
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www.protocols.io @protocolsio.bsky.social · 10/03/2026
Join our webinar to hear from researchers at the University of Rochester and City of Hope on practical strategies for improving documentation, supporting training, and increasing visibility of core facility work. April 9 | 11 AM EST | 5 PM CET Register here: go.springernature.com/enhancing-im...
Webinar poster: “Enhancing Impact in Core Facilities,” April 9, 11 AM EST / 5 PM CET.
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Radcliffe Science Library @radcliffescilib.bsky.social · 26/02/2026
Last month, we highlighted Protocols.io as a new resource available at @bodleian.ox.ac.uk. Next week, as part OxFOS, you can sign up and join the Springer Nature team for a training session introducing the platform to researchers either in person here at the RSL or online go.glam.ox.ac.uk/proto
Protocol.io logo
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Kelvin Lau @klausenhauser on 🙅‍♂️🐘🪡 @klausenhauser.bsky.social · 20/02/2026
Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase! dx.doi.org/10.17504/protocols.io.261ged7xov47/v2 Thank you @jcoker10.bsky.social and Michael Lim!
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Francis Crick Institute Library & Information Services @fci-library.bsky.social · 17/02/2026
We've updated our protocols.io page to highlight a couple of the protocols published by Crick authors recently. If you're at the Crick, don't forget you can get your flat pdfs converted to the protocols.io format at no cost! 😀 www.protocols.io/organization...
protocols.io
Francis Crick Institute
The Francis Crick Institute (formerly the UK Centre for Medical Research and Innovation) is a biomedical research centre in London, which was established in 2010 and opened in 2...
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PLOS One @plosone.org · 10/02/2026
A Lab Protocol by Omid Gholamalamdari and Stephanie C. Weber details an optimized method for labeling of nascent RNA transcripts in the C. elegans intestine, enabling spatial analysis of transcription dynamics. plos.io/3Ox4rnO @protocolsio.bsky.social link: plos.io/3O9Jv6g
plos.io
Labeling of nascent RNA in the C. elegans intestine
Transcriptional regulation in C. elegans has been difficult to study at the level of nascent RNA because nucleotide analogs do not readily penetrate the cuticle. Here, we establish an ex vivo…
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Daniel Bolnick @danielbolnick.bsky.social · 03/02/2026
New @protocolsio.bsky.social post describing our new protocol for generating intestinal organoids for #stickleback: www.protocols.io/view/generat... With work by postdoc Arshad Padhiar, funded by @moorefound.bsky.social
protocols.io
Generation of Stickleback intestinal organoid
Intestinal organoids offer significant advantages over conventional 2D cultures, providing a physiologically relevant 3D architecture that enables more realistic disease modelin...
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Sara Zufan, PhD, MPH @exsciled.bsky.social · 03/02/2026
Given the recent #Nipah outbreak in India, here are two #Nanopore protocols for NiV genomic sequencing in support of rapid outbreak response: IPC: www.protocols.io/view/sequenc... icddr,b/CDC: journals.asm.org/doi/10.1128/...
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Springer Nature @springernature.com · 15/01/2026
💡 Did you know? Methods are among the most cited research outputs. For #LoveMethods26, join experts for interactive sessions: 👉 Jan 21: @protocolsio.bsky.social Hackathon 👉 Jan 22: Methods Mythbusting Panel spklr.io/633288eAVM #OpenScience
Promotional graphic for Springer Nature and protocols.io featuring an event announcement. The left side has a light blue background with the Springer Nature logo at the top and text reading: “Join experts for interactive sessions. Jan 21: protocols.io Hackathon. Jan 22: Methods Mythbusting Panel.” A dark blue button below says “Register today.” The right side shows the protocols.io logo and a large text design that says “I ♥ Methods” with a red heart symbol, followed by “Love Methods Week” and the hashtag #LoveMethods26.
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Francis Crick Institute Library & Information Services @fci-library.bsky.social · 19/01/2026
Happy International Love Methods week! The Crick partners with @protocolsio.bsky.social to share open methods, and if you happen to be a Crick researcher, you can use their protocol writing up service for free (while codes last!) www.protocols.io/we-enter-pro...
protocols.io
We will import and format your protocol
Paper protocols floating on your benchtops? We can digitize them for you!
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www.protocols.io @protocolsio.bsky.social · 09/01/2026
Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:
protocols.io
Join protocols.io and Springer Nature editors at Love Methods Week 2026
Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...
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www.protocols.io @protocolsio.bsky.social · 09/01/2026
Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:
protocols.io
Join protocols.io and Springer Nature editors at Love Methods Week 2026
Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...
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Radcliffe Science Library @radcliffescilib.bsky.social · 07/01/2026
Our promoted new resource for January is Protocols.io. Using Protocols.io researchers can create and share detailed experimental protocols with colleagues or the wider scientific community www.protocols.io
Graphic representing our monthly New Resource promotion featuring the name of the Library and this months chosen product - Protocols.io
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Nature Protocols @natprot.nature.com · 15/12/2025
Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs at Love Methods Week 2026excelscior.uc.pt/love-methods...6/ #LoveMethods26
protocols.io
Methods Mythbusting: Insights from Journal Editors and protocols.io
This webinar will be held at: 3pm CET / 2pm GMT / 9am EST / 6am PST Join us for a lively session where we tackle common misconceptions about protocols and methods publishing. H...
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GenomiqueENS @genomiqueens.bsky.social · 10/12/2025
🎉 We are thrilled to announce that our protocol “Library construction for ONT long read cDNA”, developed at our GenomiqueENS core facility, is available on protocol.io. dx.doi.org/10.17504/protocols.io.4r3l21oepg1y/v1
protocol.io
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GenomiqueENS @genomiqueens.bsky.social · 10/12/2025
🧬Searching for a Nanopore RNA-seq protocol that generates longer reads than the manufacturer’s protocol, and applicable for low-input material ? 🤔
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Jacques Dainat @jacquesdainat.bsky.social · 26/11/2025
Really liking the idea and output of GAQETv2 - such a cool tool for structural genome annotation QC!! www.protocols.io/view/structu...
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Emma Ganley @emmaganley.bsky.social · 27/11/2025
Exciting news: Premium protocols.io is now available via Research4Life to researchers in low- & middle-income countries—at no or low cost. 🌟 Full Springer Nature press release: group.springernature.com/gp/group/med... #OpenScience #Reproducibility #GlobalCollaboration #Research4Life #protocolsio
group.springernature.com
New collaboration empowers thousands of researchers to share research methods, improve reproducibility and drive global scientific progress | Springer Nature Group | Springer Nature
Heidelberg | London, 27 November 2025 Springer Nature and Research4Life extend partnership to include access to protocols.io for scientists in over
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Springer Nature @springernature.com · 27/11/2025
Springer Nature has extended its partnership with @r4lpartnership.bsky.social to provide researchers in more than 90 low-middle-income countries with no-cost or low-fee access to premium features on @protocolsio.bsky.social. Learn more: group.springernature.com/gp/group/med... #Research 🧪
The image promotes a new collaboration empowering researchers to share methods, improve reproducibility, and drive global scientific progress. It includes a close-up of a computer chip with a brain illustration and the Springer Nature logo.
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Lenny Teytelman @lteytelman.bsky.social · 18/11/2025
Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub librehub.github.io) is an amazing initiative from @makertobey.bsky.social and colleagues!
librehub.github.io
The Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub)
...
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Aureliano Bombarely @bombarelya.bsky.social · 18/11/2025
We have done our first @protocolsio.bsky.social. Are you interested about #genome_annotation quality evaluation? We have developed a tool called #GAQET2 (github.com/victorgcb198...) which runs several tools like #AGAT, #BUSCO, and many other tools. You can find it at www.protocols.io/view/structu...
github.com
GitHub - victorgcb1987/GAQET2
Contribute to victorgcb1987/GAQET2 development by creating an account on GitHub.
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Tobias Wenzel @makertobey.bsky.social · 18/11/2025
It was great to hear from @lteytelman.bsky.social about Protocols.io today for our LIBRE hub channel on open science! We already have a tutorial in Spanish: youtu.be/RuBKWouEyL8?...
youtu.be
Construyendo protocolos científicos reproducibles y abiertos con Protocols.io
YouTube video by LIBRE hub
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Barriga Lab @eliasbarrigalab.bsky.social · 11/11/2025
With the full CRISPR 7-11 protocol here :)
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Aligning Science Across Parkinson's @asapresearch.parkinsonsroadmap.org · 31/10/2025
👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio! Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉 ➡️ Mouse Perfusions (bit.ly/4qH6rIY) ➡️ Y-Maze (bit.ly/47aITTC) ➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)
Graphic with a dark teal background featuring three illustrated circular icons representing different research teams.

On the left, Team Kordower icon shows colorful antibody shapes labeled “Neuro-Immune Interactions.”

In the center, Team Liddle icon depicts a stylized brain with interconnected circuitry labeled “Circuitry and Brain-Body Interactions.”

On the right, Team Rio icon shows a human head outline with a DNA strand inside, labeled “PD Functional Genomics.”

A thin gradient line runs along the bottom, shifting from purple to blue to green, with the ASAP logo in the lower right corner.
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Ronan Doyle @ronandoyle.bsky.social · 20/10/2025
Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now: www.medrxiv.org/content/10.1... ✅ Now implemented in 6 NHS hospitals ✅ Increased sensitivity ✅ Increased pathogen genome coverage (1/3)
medrxiv.org
Optimisation of whole cell human depletion provides increased sensitivity and microbial genome coverage from respiratory metagenomic assays
Metagenomics is being adopted worldwide as a laboratory developed test. We have previously reported a unified metagenomic method for the direct detection of microorganisms from respiratory samples, de...
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Emma Ganley @emmaganley.bsky.social · 09/10/2025
Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF: 🔗 www.researchprofessionalnews.com/rr-news-uk-r... Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.
researchprofessionalnews.com
‘REF needs dedicated section for research methods’ - Research Professional News
Hidden REF Festival: Conference hears plea to fill gap in Research Excellence Framework
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www.protocols.io @protocolsio.bsky.social · 06/10/2025
Explore our new featured protocols protocols.io/welcome
protocols.io
Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
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Lenny Teytelman @lteytelman.bsky.social · 02/10/2025
I've been dreaming about this for a decade and it's now live! "Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io" www.protocols.io/blog/global-...
protocols.io
Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io
Research is a global endeavour, yet the expectation to communicate in English often creates barriers and an additional burden for non-native speakers. At the lab bench or in t...
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