Reposted by www.protocols.ioErin Hahn (she/her) @erinhahn.bsky.social · 16/09/2026Join us next week for a chat about Reproducible methods using @protocolsio.bsky.social 🔬 Register today! 👇 021
Reposted by www.protocols.ioStephanie Hampton @sehampton.bsky.social · 17/09/2026Winter #limnology sampling is on the horizon! Our Winter Limnology Network team recently posted our official protocols for sampling under the ice in seasonally frozen lakes - check them out, use them, go get some winter data! www.protocols.io/view/limnolo... 072
Reposted by www.protocols.ioNortheastern University Library @northeasternlib.bsky.social · 11/09/2026Get your questions about protocols.io answered during expert Mericka McCabe's office hours. 📆 Friday, September 18 ⏰ 9 - 10 a.m. EDT 📍 Snell Library 215 🔗 northeastern.libcal.com/event/17469987northeastern.libcal.comMeet with an expert: protocols.ioNortheastern researchers have Premium access to protocols.io, an experimental workflow tool and open methods repository that allows you to find, create, store, run, and share... 111
www.protocols.io @protocolsio.bsky.social · 09/09/2026Enabling Reproducible Science Methods locked in static documents don't scale. Join researchers from CSIRO, ANU and Waters Biosciences for a free session on making research workflows reproducible and collaborative. Register here: go.springernature.com/anz-webinar-... 000
Reposted by www.protocols.iosmahtnetwrk.bsky.social @smahtnetwrk.bsky.social · 28/08/2026Explore a step-by-step NanoSeq library preparation protocol using mung bean nuclease for whole-genome duplex sequencing. Optimized across 15+ human tissues, the method enables improved genome coverage for high-sensitivity somatic mutation detection. www.protocols.io/view/optimiz...protocols.ioOptimized Mung Bean Nuclease Nanoseq Library Preparation Protocol for Whole Genome SequencingOptimized Mung Bean Nuclease Nanoseq Library Preparation Protocol for Whole Genome Sequencing. Duplex. Read full protocol, steps, and materials on protocols.io 052
Reposted by www.protocols.ioCarl Zimmer @carlzimmer.com · 27/08/2026The lab is a mysterious place--where some people have "magic hands," and some experiments just won't work. Here's my story about the unspoken knowledge of science, and the struggle to share it. Gift link: nyti.ms/3UccF7Z 1021158
Reposted by www.protocols.ioSPARC @sparcopen.bsky.social · 26/08/2026Read our new impact story on Michael J. Fox Foundation & @asapresearch.parkinsonsroadmap.org. Updated #OpenScience requirements aim to accelerate discovery & improve treatment for Parkinson’s disease. It's an inspiration to other @orfg.bsky.social members. sparcopen.org/impact-story...sparcopen.orgParkinson’s Research Funders Lead the Way with Progressive Open Access and Open Science Policies - SPARC 072
www.protocols.io @protocolsio.bsky.social · 21/08/2026@springernature.com is sponsoring Lab of the Future Conference! Our protocols.io, Experimental Workflow Solutions and AI Growth & Partnerships teams will be there. Let’s connect on digital workflows and research ecosystems accelerating discovery. Join us there: www.lab-of-the-future.com/europe 051
Reposted by www.protocols.ioMax Farnworth @maxfarnworth.bsky.social · 12/08/2026Our book chapter from 7 (?!?!) years ago, is now also available as protocols.io version! That's pretty neat. www.protocols.io/view/the-red...protocols.ioThe Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tr...The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development. . Read full protocol, s... 173
Reposted by www.protocols.ioIgnacio J. Melero-Jiménez @ignamelero.bsky.social · 21/07/2026🧬 We’ve shared an open-access protocol for cost-effective, high-quality DNA extraction from cyanobacteria and microalgae. We hope it will be useful to the research community! Developed with @Raul_MontanezM. doi.org/10.17504/pro... #Cyanobacteria #Microalgae #DNAExtraction #Genomics #ProtocolsIOdoi.orgLab protocol: DNA extraction from cyanobacteria and microalgaeLab protocol: DNA extraction from cyanobacteria and microalgae. Photosynthetic microorganisms are key. Read full protocol, steps, and materials on protocols.io 092
Reposted by www.protocols.ioEmory CIDMATH @emorycidmath.bsky.social · 22/07/2026Access the full protocol on protocols.io: www.protocols.io/view/rsv-a-a...protocols.ioBring structure to your research - protocols.ioA secure platform to develop, share, and discover reproducible research methods, protocols, and workflows across teams and the global scientific community. 031
Reposted by www.protocols.ioEmory CIDMATH @emorycidmath.bsky.social · 22/07/2026"RSV-A and RSV-B Amplicon Sequencing from Solid-Phase Wastewater Using the NEBNext® RSV Primer Module" is a novel protocol by CIDMATH researchers Said Rachida, Alaa Ahmed, Hannah Cayla D., Abby Paulos, Orlando Sablon, Marlene Wolfe, and Anne Piantadosi. 122
Reposted by www.protocols.ioBen Pascoe @benizao.bsky.social · 26/06/2026Open science has been central to this project from the beginning. We've made our laboratory and sequencing protocols openly available so that others can build on the framework. 🧫 Laboratory protocols www.protocols.io/view/getcamp... 🧬 Sequencing protocols www.protocols.io/view/getcamp...protocols.ioGetCampy_Laboratory Procedures V.1GetCampy_Laboratory Procedures. This standard operating procedure (SOP) outlines the preparation, qual. Read full protocol, steps, and materials on protocols.io 121
Reposted by www.protocols.ioBen Pascoe @benizao.bsky.social · 26/06/2026🧵 New paper! 🚨 Delighted to see our GETCampy-Africa protocol published in BMJ Open. *Protocols for genomic epidemiology and source attribution of enteric bacteria causing diarrhoeal disease across Africa* @bmj.com @bmjmedicine.bsky.social 📄 bmjopen.bmj.com/content/16/6... 👇bmjopen.bmj.comProtocols for genomic epidemiology and source attribution of enteric bacteria causing diarrhoeal disease across AfricaIntroduction Enteric bacterial pathogens are a major cause of diarrhoeal disease in low-income and middle-income countries, with complex transmission pathways involving human, animal and environmental... 153
Reposted by www.protocols.ioTom Pennance @schistosoma.bsky.social · 27/05/2026A protocol for performing whole genome sequencing of individual Schistosoma haematobium larva stored on FTA cards. Following this protocol (inc. diagnosis), with list price consumables, I currently estimate one can achieve a ~50x coverage of each genome for ~£104 www.protocols.io/view/schisto...protocols.ioSchistosoma haematobium larva FTA-to-WGSSchistosoma haematobium larva FTA-to-WGS. This protocol describes the isolation of individual schistos. Read full protocol, steps, and materials on protocols.io 031
Reposted by www.protocols.ioZebrafish Rock! @zebrafishrock.bsky.social · 25/04/2026As we near the end of #EarthWeek, a reminder that #zebrafish do not need sterile dishes! Save money and reduce plastic consumption by sanitizing used dishes with hot water or dilute bleach 🧫💦 For example, you could follow this protocol from the Fish Floor at UCL: www.protocols.io/view/washing... 🐟 🧪 25611
Reposted by www.protocols.ioMatt Lycas @lycasworks.bsky.social · 27/04/2026www.protocols.io/view/cryo-ex... New protocol alert!! Cryo-expansion microscopy of C. elegans and Tardigrades using the high pressure freezer!protocols.ioCryo-Expansion Microscopy of C. elegans and TardigradesCryo-Expansion Microscopy of C. elegans and Tardigrades. Expansion microscopy (ExM) improves imaging r. Read full protocol, steps, and materials on protocols.io 0318
Reposted by www.protocols.ioDoug Dyer @douglaspdyer.bsky.social · 01/05/2026delighted to share our new pre-print developing label-free glycosaminoglycan analysis of diverse complex biological samples, led by @h-davies-s.bsky.social working closely with David Knight and Rebecca Miller alongisde our recent recruit Samie Dalgarno www.biorxiv.org/content/10.6...biorxiv.org 4177
www.protocols.io @protocolsio.bsky.social · 28/04/2026Modern research is increasingly collaborative, creating a need for clearer, shared workflows. Join us on May 5, 2026 at 14:00 CET | 13:00 GMT for a webinar on how research teams are adapting and collaborating at scale. Register here: go.springernature.com/int1/pf040/e... 020
www.protocols.io @protocolsio.bsky.social · 22/04/2026We’re transforming research protocols from static documents into dynamic, reusable workflows. A key part is making large collections of trusted methods — including Protocol Exchange and Springer Protocols — available in protocols.io format, so researchers can reuse, adapt, and build on them.protocols.ioEvolving protocols: From static to dynamic with protocols.ioSharing research protocols is a cornerstone of open science, and protocols.io, part of Springer Nature, continues to evolve as a secure, modern platform for... 021
Reposted by www.protocols.ioSpringer Nature @springernature.com · 29/03/2026Strengthening transparency and reproducibility starts with sharing research work openly. Our latest blogs offer guidance for: > How to share research code openly: spklr.io/63329Eynzz > How to share research protocols and methods openly: spklr.io/63322EynzO 012
www.protocols.io @protocolsio.bsky.social · 19/03/2026Attending ABRF 2026 (@abrf.bsky.social)? Connect with protocols.io at Booth 710. We're engaging with core facilities about common priorities and challenges, including how to demonstrate impact and receive credit. If this resonates, schedule a short discussion with our team: abrf26.youcanbook.me 011
Reposted by www.protocols.ioNoura Maziak @nmaziak.bsky.social · 10/03/2026Excited to share my PhD work! The question: How is genome organization established? Here, we developed a low-input Micro-C method, Pico-C, and used it to map chromatin architecture across early stages of fly development. Here’s a quick Blue-torial 🙂 (1/n) www.nature.com/articles/s41...nature.comThree-dimensional genome reorganization foreshadows zygotic genome activation in Drosophila - Nature GeneticsPico-C, a low-input Micro-C approach, reveals that dynamic three-dimensional genome folding precedes zygotic genome activation in Drosophila. 17024
www.protocols.io @protocolsio.bsky.social · 10/03/2026Join our webinar to hear from researchers at the University of Rochester and City of Hope on practical strategies for improving documentation, supporting training, and increasing visibility of core facility work. April 9 | 11 AM EST | 5 PM CET Register here: go.springernature.com/enhancing-im... 021
Reposted by www.protocols.ioRadcliffe Science Library @radcliffescilib.bsky.social · 26/02/2026Last month, we highlighted Protocols.io as a new resource available at @bodleian.ox.ac.uk. Next week, as part OxFOS, you can sign up and join the Springer Nature team for a training session introducing the platform to researchers either in person here at the RSL or online go.glam.ox.ac.uk/proto 032
Reposted by www.protocols.ioKelvin Lau @klausenhauser on 🙅♂️🐘🪡 @klausenhauser.bsky.social · 20/02/2026Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase! dx.doi.org/10.17504/protocols.io.261ged7xov47/v2 Thank you @jcoker10.bsky.social and Michael Lim! 33212
Reposted by www.protocols.ioFrancis Crick Institute Library & Information Services @fci-library.bsky.social · 17/02/2026We've updated our protocols.io page to highlight a couple of the protocols published by Crick authors recently. If you're at the Crick, don't forget you can get your flat pdfs converted to the protocols.io format at no cost! 😀 www.protocols.io/organization...protocols.ioFrancis Crick InstituteThe Francis Crick Institute (formerly the UK Centre for Medical Research and Innovation) is a biomedical research centre in London, which was established in 2010 and opened in 2... 033
Reposted by www.protocols.ioPLOS One @plosone.org · 10/02/2026A Lab Protocol by Omid Gholamalamdari and Stephanie C. Weber details an optimized method for labeling of nascent RNA transcripts in the C. elegans intestine, enabling spatial analysis of transcription dynamics. plos.io/3Ox4rnO @protocolsio.bsky.social link: plos.io/3O9Jv6gplos.ioLabeling of nascent RNA in the C. elegans intestineTranscriptional regulation in C. elegans has been difficult to study at the level of nascent RNA because nucleotide analogs do not readily penetrate the cuticle. Here, we establish an ex vivo… 054
Reposted by www.protocols.ioDaniel Bolnick @danielbolnick.bsky.social · 03/02/2026New @protocolsio.bsky.social post describing our new protocol for generating intestinal organoids for #stickleback: www.protocols.io/view/generat... With work by postdoc Arshad Padhiar, funded by @moorefound.bsky.socialprotocols.ioGeneration of Stickleback intestinal organoidIntestinal organoids offer significant advantages over conventional 2D cultures, providing a physiologically relevant 3D architecture that enables more realistic disease modelin... 052
Reposted by www.protocols.ioSara Zufan, PhD, MPH @exsciled.bsky.social · 03/02/2026Given the recent #Nipah outbreak in India, here are two #Nanopore protocols for NiV genomic sequencing in support of rapid outbreak response: IPC: www.protocols.io/view/sequenc... icddr,b/CDC: journals.asm.org/doi/10.1128/... 022
Reposted by www.protocols.ioSpringer Nature @springernature.com · 15/01/2026💡 Did you know? Methods are among the most cited research outputs. For #LoveMethods26, join experts for interactive sessions: 👉 Jan 21: @protocolsio.bsky.social Hackathon 👉 Jan 22: Methods Mythbusting Panel spklr.io/633288eAVM #OpenScience 064
Reposted by www.protocols.ioFrancis Crick Institute Library & Information Services @fci-library.bsky.social · 19/01/2026Happy International Love Methods week! The Crick partners with @protocolsio.bsky.social to share open methods, and if you happen to be a Crick researcher, you can use their protocol writing up service for free (while codes last!) www.protocols.io/we-enter-pro...protocols.ioWe will import and format your protocolPaper protocols floating on your benchtops? We can digitize them for you! 063
Reposted by www.protocols.iowww.protocols.io @protocolsio.bsky.social · 09/01/2026Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:protocols.ioJoin protocols.io and Springer Nature editors at Love Methods Week 2026Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth... 075
www.protocols.io @protocolsio.bsky.social · 09/01/2026Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:protocols.ioJoin protocols.io and Springer Nature editors at Love Methods Week 2026Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth... 075
Reposted by www.protocols.ioRadcliffe Science Library @radcliffescilib.bsky.social · 07/01/2026Our promoted new resource for January is Protocols.io. Using Protocols.io researchers can create and share detailed experimental protocols with colleagues or the wider scientific community www.protocols.io 031
Reposted by www.protocols.ioNature Protocols @natprot.nature.com · 15/12/2025Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs at Love Methods Week 2026excelscior.uc.pt/love-methods...6/ #LoveMethods26protocols.ioMethods Mythbusting: Insights from Journal Editors and protocols.ioThis webinar will be held at: 3pm CET / 2pm GMT / 9am EST / 6am PST Join us for a lively session where we tackle common misconceptions about protocols and methods publishing. H... 042
Reposted by www.protocols.ioGenomiqueENS @genomiqueens.bsky.social · 10/12/2025🎉 We are thrilled to announce that our protocol “Library construction for ONT long read cDNA”, developed at our GenomiqueENS core facility, is available on protocol.io. dx.doi.org/10.17504/protocols.io.4r3l21oepg1y/v1protocol.io 112
Reposted by www.protocols.ioGenomiqueENS @genomiqueens.bsky.social · 10/12/2025🧬Searching for a Nanopore RNA-seq protocol that generates longer reads than the manufacturer’s protocol, and applicable for low-input material ? 🤔 132
Reposted by www.protocols.ioJacques Dainat @jacquesdainat.bsky.social · 26/11/2025Really liking the idea and output of GAQETv2 - such a cool tool for structural genome annotation QC!! www.protocols.io/view/structu... 021
Reposted by www.protocols.ioEmma Ganley @emmaganley.bsky.social · 27/11/2025Exciting news: Premium protocols.io is now available via Research4Life to researchers in low- & middle-income countries—at no or low cost. 🌟 Full Springer Nature press release: group.springernature.com/gp/group/med... #OpenScience #Reproducibility #GlobalCollaboration #Research4Life #protocolsiogroup.springernature.comNew collaboration empowers thousands of researchers to share research methods, improve reproducibility and drive global scientific progress | Springer Nature Group | Springer NatureHeidelberg | London, 27 November 2025 Springer Nature and Research4Life extend partnership to include access to protocols.io for scientists in over 032
Reposted by www.protocols.ioSpringer Nature @springernature.com · 27/11/2025Springer Nature has extended its partnership with @r4lpartnership.bsky.social to provide researchers in more than 90 low-middle-income countries with no-cost or low-fee access to premium features on @protocolsio.bsky.social. Learn more: group.springernature.com/gp/group/med... #Research 🧪 022
Reposted by www.protocols.ioLenny Teytelman @lteytelman.bsky.social · 18/11/2025Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub librehub.github.io) is an amazing initiative from @makertobey.bsky.social and colleagues!librehub.github.ioThe Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub)... 133
Reposted by www.protocols.ioAureliano Bombarely @bombarelya.bsky.social · 18/11/2025We have done our first @protocolsio.bsky.social. Are you interested about #genome_annotation quality evaluation? We have developed a tool called #GAQET2 (github.com/victorgcb198...) which runs several tools like #AGAT, #BUSCO, and many other tools. You can find it at www.protocols.io/view/structu...github.comGitHub - victorgcb1987/GAQET2Contribute to victorgcb1987/GAQET2 development by creating an account on GitHub. 1126
Reposted by www.protocols.ioTobias Wenzel @makertobey.bsky.social · 18/11/2025It was great to hear from @lteytelman.bsky.social about Protocols.io today for our LIBRE hub channel on open science! We already have a tutorial in Spanish: youtu.be/RuBKWouEyL8?...youtu.beConstruyendo protocolos científicos reproducibles y abiertos con Protocols.ioYouTube video by LIBRE hub 032
Reposted by www.protocols.ioBarriga Lab @eliasbarrigalab.bsky.social · 11/11/2025With the full CRISPR 7-11 protocol here :) 0105
Reposted by www.protocols.ioAligning Science Across Parkinson's @asapresearch.parkinsonsroadmap.org · 31/10/2025👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio! Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉 ➡️ Mouse Perfusions (bit.ly/4qH6rIY) ➡️ Y-Maze (bit.ly/47aITTC) ➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR) 011
Reposted by www.protocols.ioRonan Doyle @ronandoyle.bsky.social · 20/10/2025Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now: www.medrxiv.org/content/10.1... ✅ Now implemented in 6 NHS hospitals ✅ Increased sensitivity ✅ Increased pathogen genome coverage (1/3)medrxiv.orgOptimisation of whole cell human depletion provides increased sensitivity and microbial genome coverage from respiratory metagenomic assaysMetagenomics is being adopted worldwide as a laboratory developed test. We have previously reported a unified metagenomic method for the direct detection of microorganisms from respiratory samples, de... 175
Reposted by www.protocols.ioEmma Ganley @emmaganley.bsky.social · 09/10/2025Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF: 🔗 www.researchprofessionalnews.com/rr-news-uk-r... Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.researchprofessionalnews.com‘REF needs dedicated section for research methods’ - Research Professional NewsHidden REF Festival: Conference hears plea to fill gap in Research Excellence Framework 152
www.protocols.io @protocolsio.bsky.social · 06/10/2025Explore our new featured protocols protocols.io/welcomeprotocols.ioBring structure to your research - protocols.ioA secure platform for developing and sharing reproducible methods. 141
Reposted by www.protocols.ioLenny Teytelman @lteytelman.bsky.social · 02/10/2025I've been dreaming about this for a decade and it's now live! "Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io" www.protocols.io/blog/global-...protocols.ioGlobal Collaboration Made Easier: Multilingual Protocols Now Available on protocols.ioResearch is a global endeavour, yet the expectation to communicate in English often creates barriers and an additional burden for non-native speakers. At the lab bench or in t... 051