Genetic code expansion can be limited by the need to supply non-standard amino acids. The authors design bacteria that perform integrated steps of converting supplemented aldehydes to L-phenylalanine ...

Abstract. Genetic circuits for model bacteria often perform poorly in non-canonical hosts, limiting the deployment of regulatory programs in industrially r

Metabolic engineering calls for new bacterial chassis that combine robust metabolic and physiological traits with genetic tractability. We developed P…

Covering: up to August 2024 Enzymes play an essential role in synthesizing value-added chemicals with high specificity and selectivity. Since enzymes utilize substrates derived from renewable…

The rhizobiome are the support systems of plants. These soil microbes living around plant roots help plants during stress like drought and heat periods. Learn how the TOLERATE project is using bioaugmentation and microbe engineering boost crop resilience

Redox homeostasis is central to microbial physiology and stress adaptation, yet the functional roles of transhydrogenases remain poorly understood beyond a few organisms. In this study, we systematica...

Synthetic one-carbon assimilation could contribute to a more sustainable and circular carbon economy, but much work in this field has focused on model microorganisms. Here the authors provide their perspective on the potential value of non-model microbes, and how that potential could be realised.

Soluble C1 feedstocks, such as formate and methanol, have gained attention as sustainable substrates for biotechnology, with the potential to reduce greenhouse gas emissions and reliance on sugar-based resources. Despite their promise, the metabolic ...

Soluble C1 feedstocks, such as formate and methanol, have gained attention as sustainable substrates for biotechnology, with the potential to reduce greenhouse gas emissions and reliance on sugar-based resources. Despite their promise, the metabolic ...

Climate change is intensifying drought events, putting global food production under pressure. Within the EU-funded TOLERATE project, Work Package 3 (WP3), led by Bioclear earth, and in collaboration w...

To advance sustainable bioproduction, we engineered Pseudomonas putida for methanol assimilation via modular synthetic serine cycle variants. By combining native and synthetic functions, we enabled me...

By designing synthetic toolkits for contact-based signaling (MARS) and cell-cell adhesion (SATURN), we program yeast to form multicellular structures and perform complex tasks, like building logic cir...

The development of a modular, phage-based expression system optimized for Pseudomonas putida enables stringent, tunable and high yield expressions with minimal cytotoxicity.

Howard et al. demonstrate that clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa can encode functional plastic-degrading enzymes and that these enzymes can influence bacterial bio...

Nature Communications - The association and dissociation dynamics of the ECF transporter complex for vitamin B12 are visualized by single-molecule FRET, highlighting the original transport...

One-carbon (C1) substrates are attractive feedstocks for biological upgrading as part of a circular, carbon-negative bioeconomy. Nature has evolved a …

Abstract. Synthetic C1 assimilation holds the promise of facilitating carbon capture while mitigating greenhouse gas emissions, yet practical implementatio

All 32 diguanylate cyclase (DGC)-encoding genes of P. aeruginosa PA14 were interrupted by premature STOP codons, introduced through a multiplexed cytosine base-editor. The synthesis of c-di-GMP was i...

Constructing an efficient microbial cell factory still requires exploring and selecting various host strains, as well as identifying the best-suited metabolic engineering strategies, which demand sign...

The urgent need for a circular carbon economy has driven research into sustainable substrates, including one-carbon (C1) compounds. The non-pathogenic soil bacterium Pseudomonas putida is a promising ...

Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS, demonstrating their use for ...

Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS, demonstrating their use for ...