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SEVA Plasmids

@sevaplasmids.bsky.social
651 followers 470 following 213 posts

A non-profit Open Access repository of standardised plasmids following the SEVA format. Check out our database and request your #plasmid free of charge #synbio seva-plasmids.com

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SEVA Plasmids @sevaplasmids.bsky.social · 28/07/2026
Tke5: A new weapon against crop-killing bacteria! 🌾⚠️ This toxin kills harmful plant pathogens by forming pores in their membranes. 🦠 #SEVA ⭕ allowed controlling its expression safely during the study. Read more: shre.ink/31qa
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SEVA Plasmids @sevaplasmids.bsky.social · 21/07/2026
PlasmidGPT 🤖 A new step for plasmid design If we combine data-driven modeling with standardized engineering practices, we can make biological design more intuitive, efficient, and expansive. Read the paper👉 shre.ink/31bJ #SEVAplasmids #SynBio #Standardization #GenomeEngineering
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SEVA Plasmids @sevaplasmids.bsky.social · 14/07/2026
A SEVA plasmid name ⭕ can tell you all you need to know. Just read the code: 🔹Selection marker 🔹Replication module 🔹Cargo 🔹Additional gadgets Standards make life easier. Learn more about nomenclature 👉https://acortar.link/XZmJVZ
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SEVA Plasmids @sevaplasmids.bsky.social · 30/06/2026
An automated workflow for high-precision genotyping of transposon variants with minimal manual intervention 🚫🤲🏼 Once again, #SEVA⭕✨steps forward to assure scalability and reproducibility for the #SynBio community. Read the study here 👉 bit.ly/4vAkSAb
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SEVA Plasmids @sevaplasmids.bsky.social · 23/06/2026
Generative AI is changing synthetic biology 🧬🤖 with an immense potential for plasmid design⭕ The era of precision design is just around the corner⚙️✨ Read more here 👉 bit.ly/4uqPxz1
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SEVA Plasmids @sevaplasmids.bsky.social · 16/06/2026
SEVA’s ⭕ LEGO style! Build your plasmid: 1️⃣ Pick a Marker. 2️⃣ Select an Origin. 3️⃣ Snap in a Gadget. 4️⃣ Add the Cargo. Start: 👉 acortar.link/G9uSJA
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SEVA Plasmids @sevaplasmids.bsky.social · 02/06/2026
Pooled rAAV libraries might be noisier than we think! This study reveals that packaging recombinant AAV libraries as a pool leads to extensive chimerism due to "barcode swapping" 🧬🔀🧬 At #SEVA ⭕, we believe that standardisation can be a good strategy to prevent this. Read here 👉 doi.org/q42d
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SEVA Plasmids @sevaplasmids.bsky.social · 26/05/2026
Rare codons slowing down your protein expression? 🛑 pCODE plasmids are an elegant solution to rescue recombinant protein production by overexpressing tRNAs! ✨ Proudly, #SEVAplasmids⭕ were used to design this new system. Check out 👉 doi.org/q42c
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SEVA Plasmids @sevaplasmids.bsky.social · 19/05/2026
🧬 Balancing genes in one cell has always been a great challenge! 🧑‍🔬 This review shows the shift from simple co-expression setups to more complex systems. It highlights the contribution of #SEVA⭕✨ as a key step toward modular, programmable designs. Read here👉 doi.org/q4z9
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SEVA Plasmids @sevaplasmids.bsky.social · 30/04/2026
🧑‍🔬 "The question is no longer whether labs can automate, but how far they should go". A recent article in R&D World magazine discusses the future of laboratory work and the impact of AI. 👉
rdworldonline.com
A new roadmap for automation in synthetic biology
The building blocks for the autonomous lab exist, and a new framework published in SLAS Technology outlines how to get there.
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SEVA Plasmids @sevaplasmids.bsky.social · 28/04/2026
🧑‍🔬Easy to design, easy to order, free of charge… It sounds like a dream, but it is not! In SEVA plasmids we want you to be able to get the exact plasmid you need, so we created an open designing platform to create your own! Try it our yourself!👉
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
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SEVA Plasmids @sevaplasmids.bsky.social · 23/04/2026
🤔The ins and outs of membrane fusion, as told by experts from the University of Genova and Max Planck Institute. This last review brings together insights on the impact of lipid composition, environmental conditions, and fusogenic agents on this essential biological process👉 doi.org/10.1080/2374...
doi.org
Browse all journals
Browse all journals
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SEVA Plasmids @sevaplasmids.bsky.social · 21/04/2026
🧑‍🔧⭕Let’s dissect a SEVA plasmid! Built on a modular architecture, our plasmids make it easier to identify key vector elements. 🧬Origin of replication: more then 15 types 🔆Cargo region: such as cloning or CRSIPR. 💊Antibiotic marker: choose it from out of 10 possibilities. 👉 shorturl.at/04ZtK
seva-plasmids.com
FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture
Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…
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SEVA Plasmids @sevaplasmids.bsky.social · 17/04/2026
Better vector design can make all the difference 🔬 Researchers examined how plasmid vectors are optimized for gene editing and genetic engineering. They focused on 🔧function 📈efficiency 🔀and versatility in #biotech applications Just how SEVA likes it.👉 Read their insights here: doi.org/qz47
doi.org
Plasmid vectors for gene editing and genetic engineering: Design principles, efficiency, and applications
Plasmid vectors remain central to gene editing and genetic engineering, providing flexible, modular, and cost-effective platforms for genome manipulation across microbial, plant, and eukaryotic…
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SEVA Plasmids @sevaplasmids.bsky.social · 15/04/2026
Designing plasmids shouldn’t be blocking your experiment 🧬 ⭕SEVA plasmids provide a shared framework for vector construction, helping researchers work with greater flexibility and #reproducibility in biotechnology and microbial engineering. Explore our website👉
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
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SEVA Plasmids @sevaplasmids.bsky.social · 13/04/2026
🔬New model systems are helping reshape drug discovery. Nowadays there are more relevant ways to study disease biology, and improve early-stage screening. TheScientist has reviewed the latest advances in this new guide about how to use in vitro models 👉https://shorturl.at/DXbIg
shorturl.at
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SEVA Plasmids @sevaplasmids.bsky.social · 10/04/2026
Methanol as feedstock? Maybe not for us but… 🧪 Resarchers built new biology toolkit to help microbes grow using methanol, making them easier to engineer and more stable. 👉Read how they managed to do it:
doi.org
A synthetic biology toolkit for the plasmid-dependent and thermophilic methylotroph Bacillus methanolicus
Bacillus methanolicus, a unique plasmid-dependent and thermophilic methylotroph, is an ideal chassis for one-carbon (C1) biomanufacturing. Despite its…
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SEVA Plasmids @sevaplasmids.bsky.social · 08/04/2026
👩‍🔬 The #SEVA collection keeps growing thanks to community contributions. Add your constructs to our open plasmid repository and help improve reproducibility in #SynBio. ✨ Check the guidelines and join our mission 👉
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
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SEVA Plasmids @sevaplasmids.bsky.social · 31/03/2026
🔬Tired of replicating that experiment and getting different results? SEVA simplifies #SynBio with a universal vector system! All SEVA #plasmids follow a standardised format. Reproducibility starts here! ⚙️👉
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
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SEVA Plasmids @sevaplasmids.bsky.social · 27/03/2026
🧬🖨️ Boosting 3D-printed tissues! MIT engineers have developed a compact magnetic mixer that keeps bioinks uniformly mixed during printing. This makes engineered tissues more reproducible and scalable – just like SEVA likes it! Check the whole story👉
surl.li
Magnetic mixer improves 3D bioprinting
A new approach developed at MIT aims to solve a core limitation in 3D bioprinting, by actively preventing cell sedimentation within bioinks, allowing for more reliable and biologically consistent 3D…
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SEVA Plasmids @sevaplasmids.bsky.social · 25/03/2026
🔭New plasmids on the horizon! Scientists from @bfrde.bsky.social detected megaplasmids in enteropathogenic Vibrio spp. These have 80% unclassified genes! ⭕What’s more, the megaplasmids suggest a non-Vibrio origin 👉
doi.org
Megaplasmids of the enteropathogenic species Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus represent a group of novel genetic elements unrelated to other plasmids of Vibrionaceae
Vibrio (V.) parahaemolyticus is a marine-associated bacterium that has previously been linked to foodborne illness associated with seafood consumption…
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SEVA Plasmids @sevaplasmids.bsky.social · 23/03/2026
💪An easy way to gain glucose! Scientists have developed a plasmid-free and inducer-free Escherichia coli to produce glucose. This may be an interesting way of reducing expenses💸. Read how they could do it👉
doi.org
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SEVA Plasmids @sevaplasmids.bsky.social · 19/03/2026
🛠️✨We are the Standard European Vector Architecture (SEVA), a web-based resource in which you can choose the #plasmid that you need for your research. Our goal is to make #reproducibility, a reality. Check them out 👉
seva-plasmids.com
Find your plasmid
Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your canonical...
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SEVA Plasmids @sevaplasmids.bsky.social · 17/03/2026
🔬SEVA format involves a set of rules to unify the scientific community. These are visible in our #plasmid nomenclature. By order: 🦠 Antibiotic resistance marker ⭕ Origin of replication 📦 Cargo module 🛠️ Optional gadgets Learn how it works here 👉https://shorturl.at/V5ff1
seva-plasmids.com
BACKBONE MODULES & NOMENCLATURE - SEVA plasmids - Standard European Vector Architecture
Plasmid Backbone The colored arrows above the cargo and AbR modules represent the directionality of the transcription flow of that element SEVA Plasmid Nomenclature The figure sketches the expanded…
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SEVA Plasmids @sevaplasmids.bsky.social · 13/03/2026
Using photosynthesis as a work chain ☀️ Researchers aimed to engineer cells that can capture light and use it to produce valuable compounds. 🦠They successfully reprogrammed E. coli to perform efficient photobiosynthesis of several products! 👉
doi.org
Light-driven metabolic makeover
New-to-nature photometabolisms are highly intriguing for manufacturing but difficult to achieve. Now, Escherichia coli engineering integrates flavin-based photobiocatalysis with natural enzymatic reactions,...
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SEVA Plasmids @sevaplasmids.bsky.social · 11/03/2026
⭕❌What would happen if organisms with and without plasmids compete? I will give you a hint: this plasmid-bearing population can produce an allelopathic toxin that is lethal to its plasmid-free competitor, and yet, who wins?👉
doi.org
Mortality can induce limit cycles in a chemostat competition model between plasmid-bearing and plasmid-free organisms with allelopathy - Computational and Applied Mathematics
In this paper, we study a model of competition between plasmid-bearing and plasmid-free organisms in a chemostat, where the plasmid-bearing population produces an allelopathic toxin that is lethal to…
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SEVA Plasmids @sevaplasmids.bsky.social · 08/03/2026
⭕✨Standard, tailor-made, and send directly to your door food free. Too good can be true. Take a look at our website and check our vectors free of charge 👉
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
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SEVA Plasmids @sevaplasmids.bsky.social · 04/03/2026
🦠✨This bacterium can act as a “sickness traffic light”. @ctropini.bsky.social and her group from the University of British Columbia have engineered a system in which cells that normally produce GFP reduce their fluorescence under stress conditions. Discover the full story 👉https://shorturl.at/f9bpu
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SEVA Plasmids @sevaplasmids.bsky.social · 03/03/2026
❓🦠With N, what is the main cause of foodborne illness? Non-typhoidal Salmonella! In it, plasmids enhance adaptability, combining resistance and virulence. Microbiota-targeted or CRISPR-based tools can provide opportunities to disrupt plasmid dissemination. 👉Check how:
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SEVA Plasmids @sevaplasmids.bsky.social · 26/02/2026
Science anywhere? Absolutely yes! Science anyhow? No please! The #SEVA database of vector plasmids aims to improve Science replication and create a world in which high-quality science is available no matter where. Join us! 👉 seva-plasmids.com
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
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SEVA Plasmids @sevaplasmids.bsky.social · 24/02/2026
The @berkeleylab.lbl.gov presented the Global Soil Plasmidome Resource. A comprehensive dataset of 98,728 plasmid sequences amassed from 6860 terrestrial microbial communities and isolates. Check them out!👉 www.nature.com/articles/s41...
nature.com
A global soil plasmidome resource unveils functional and ecological roles of plasmids in soil microbiomes - Nature Communications
Here, the authors report the Global Soil Plasmidome Resource, a comprehensive plasmid soil catalog, that explores the roles of plasmids in microbial adaptation and host integration, highlighting…
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SEVA Plasmids @sevaplasmids.bsky.social · 23/02/2026
Replicable science requires shared standards. The #SEVA platform provides modular, standardized plasmid vectors that improve reproducibility and design. Explore the SEVA collection and order validated plasmids for free 👉 seva-plasmids.com/find-your-pl...
seva-plasmids.com
FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture
Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…
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SEVA Plasmids @sevaplasmids.bsky.social · 20/02/2026
🌍🧬 Plasmids are spreading antibiotic resistance across European urban water systems. A multi-omics survey found tens of thousands of plasmids carrying resistance genes, many previously unknown. It highlights how wastewater networks share and mobilize ARGs across borders 👉https://doi.org/qn2v
doi.org
Beyond borders: plasmids drive a shared antibiotic resistome in European urban water systems - Microbiome
Background Urban wastewater systems (UWSs) act as reservoirs and conduits for the dissemination of antibiotic resistance genes (ARGs), with plasmids playing a central role in their spread. Despite…
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SEVA Plasmids @sevaplasmids.bsky.social · 18/02/2026
🧬📊 Read this comparative review of plasmid vectors used in gene editing and genetic engineering. It highlights how designing elements such as replication origin, host range, and CRISPR compatibility affect multiple features. How important it is to make the right selection? 👉
doi.org
Comparative analysis of plasmid vectors used in gene editing and genetic engineering
Plasmid vectors are foundational tools in gene editing and genetic engineering, enabling targeted genome modification, heterologous gene expression, and metabolic pathway optimization across diverse…
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SEVA Plasmids @sevaplasmids.bsky.social · 16/02/2026
If you are a researcher working with plasmids, this is for you!👩🏾‍🔬🔬Standard, effective and ✨free✨, what else can you ask for? Take a look at our website and find your #SEVAplasmid ready to use 👉
seva-plasmids.com
FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture
Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…
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SEVA Plasmids @sevaplasmids.bsky.social · 14/02/2026
🧬🔧 New tools keep emerging for mycobacterial protein production. This plasmid-based system enables translationally gated secretion in Mycobacterium, combining riboswitch control with highly efficient extracellular export. A new layer of regulation unlocked👉
doi.org
A Versatile Plasmid System for Translational Control and Secretion of Recombinant Proteins in Mycobacteria
Recombinant protein expression in mycobacteria faces two major challenges: limited regulatory tools for inducible expression and inefficient secretion of heterologous products. In this study, we…
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SEVA Plasmids @sevaplasmids.bsky.social · 12/02/2026
A free, worldwide and standardized tool for researchers 🧑‍🔬🛠️ It seems too good to be true, but it exists! ⭕Our #SEVA plasmids accomplish all those characteristics to help scientific research succeed. Use them yourself! 👉
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
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SEVA Plasmids @sevaplasmids.bsky.social · 10/02/2026
🩸🩹You may have heard about using glue to stop bleedings. Maybe it is not the best strategy in the world, but scientists have engineered bacteria to function as therapeutic ‘living glues’ inside your body! 😳. Check how they do it 👉
doi.org
A therapeutic glue that autonomously targets gut bleeding in inflammatory bowel disease - Nature Biotechnology
We engineered bacteria to function as therapeutic ‘living glues’ that autonomously detect gastrointestinal bleeding and, in response, secrete adhesive and therapeutic proteins to enable targeted…
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SEVA Plasmids @sevaplasmids.bsky.social · 06/02/2026
⭕ Plasmids of the group lncP-1 replicate and usually persist in many bacteria, but nothing in nature comes without exception. The lncP-1𝛃 plasmid pBP136 became extinct in E.coli at the lab. Scientists from the @uidaho.bsky.social suspect which gene is guilty. 👉
doi.org
Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost
Abstract. Plasmids promote adaptation of bacteria by facilitating horizontal transfer of diverse genes, notably those conferring antibiotic resistance. Som
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SEVA Plasmids @sevaplasmids.bsky.social · 04/02/2026
🧬Plasmids are the workhorses of molecular biology, but are we really choosing them wisely? @vdlorenzo.bsky.social and Esteban Martínez-García revisit what really defines plasmid performance and why modular standards like #SEVA matter for the future of #SynBio 👉https://doi.org/10.1111/1751-7915.70273
doi.org
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SEVA Plasmids @sevaplasmids.bsky.social · 02/02/2026
Have you already found your pSEVA plasmid match for your #SynBio project? You’re just one step away! 🚀 📤 Visit our website and choose the gateway to your next experiment—free, open, and ready to use 👉https://seva-plasmids.com/contact/
seva-plasmids.com
CONTACT - SEVA plasmids - Standard European Vector Architecture
Order plasmids If you wish to order SEVA plasmids, please fill in the form below. We offer this service pro bono so it is free of charge. Large orders ( > 15 plasmids) may experience shipment delays…
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SEVA Plasmids @sevaplasmids.bsky.social · 28/01/2026
♻️🦠Tackling mixed plastics! See the potential of engineered microbial consortia to upcycle complex mixed plastic waste. Integrating transition metal-free plastic depolymerization with the strengths of each bacterial specialist and division of labor 👇 doi.org/10.1038/s414...
doi.org
Engineering microbial consortia for mixed plastic upcycling - Nature Communications
The chemical complexity of post-consumer ‘mixed’ plastic waste limits its use as a feedstock for biomanufacturing. Here the authors combine transition-metal-free plastic deconstruction with a…
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SEVA Plasmids @sevaplasmids.bsky.social · 23/01/2026
In need of a new #SEVA construct? Contributing to the community and to the plasmid repository is simple: 1. Remove unwanted and extra restriction sites 2. Flank every module with the appropriate SEVA-standard sites 3. Send your construct! 📋Check all the details seva-plasmids.com/description/...
seva-plasmids.com
Description - SEVA plasmids - Standard European Vector Architecture
What is the SEVA format? The SEVA format involves a set of rules for the physical assembly of the three basic components of plasmid vectors (origin of replication, selection marker and the business…
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SEVA Plasmids @sevaplasmids.bsky.social · 22/01/2026
🔬Investigating viral infections in a controlled way: researchers establish a cell-free T7 phage cycle on synthetic cells. A simplified but promising in vitro system for studying viral infection variables, phage-host interactions, and more. Full story👇 doi.org/10.1038/s414...
doi.org
A synthetic cell phage cycle - Nature Communications
The reconstitution of complex biological processes in cell-free systems can support the detailed characterisation of biochemical mechanisms which are difficult to probe in vivo. Here authors present…
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SEVA Plasmids @sevaplasmids.bsky.social · 20/01/2026
🦠⏩Beyond traditional organisms, broad-host-range synthetic biology offers a powerful framework for innovation, expanding chassis selection and rethinking their role in genetic design. Take a look at the perspective paper doi.org/10.1021/acss...
doi.org
Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable
Broad-host-range synthetic microbiology is redefining the role of microbial hosts in genetic design by moving beyond the traditional organisms. Historically, synthetic biology has focused on…
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SEVA Plasmids @sevaplasmids.bsky.social · 15/01/2026
Let’s break down a pSEVA plasmid’s mix-and-match available modules! 🔵 Antibiotic resistance marker 🟡 Origin of replication 🔴 Cargo 🟢 and optional gadget Check our user-friendly platform and find the plasmids with the ideal modules for your research! 👉 seva-plasmids.com/find-your-pl...
seva-plasmids.com
FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture
Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…
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SEVA Plasmids @sevaplasmids.bsky.social · 14/01/2026
🧬How selective pressures shape plasmid evolution within the host cell? Scientists have measured within-cell fitness of competing plasmids, revealing new insights into cell and plasmid dynamics and into how antibiotic resistance evolves in bacteria! Take a deeper look doi.org/10.1126/scie...
doi.org
Intracellular competition shapes plasmid population dynamics
From populations of multicellular organisms to selfish genetic elements, conflicts between levels of biological organization are central to evolution. Plasmids are extrachromosomal, self-replicating…
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SEVA Plasmids @sevaplasmids.bsky.social · 09/01/2026
👩‍🔬Researchers use a SEVA plasmid to help develop citrOgen. A rapid platform that repurposes Citrobacter rodentium to present antigens from high-priority human pathogens, triggering the host to produce protective antibodies🛡️. 👉https://doi.org/10.1038/s41467-025-63922-0
doi.org
citrOgen: a synthesis-free polysaccharide and protein antigen-presentation to antibody-induction platform - Nature Communications
Bacterial antigens, such as lipopolysaccharides, are complex structures which remain difficult to synthesise or purify for antibody generation. Here, authors present a platform technology using…
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SEVA Plasmids @sevaplasmids.bsky.social · 07/01/2026
A standardised plasmid repository for experimental replicability ⭕. Check #SEVAplasmids database and join the movement! 👉 seva-plasmids.com/find-your-pl...
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SEVA Plasmids @sevaplasmids.bsky.social · 31/12/2025
✨And with that the 2025 season comes to an end... But at SEVA we continue working for a replicable and reliable science🤝 We want to thank you all for your support through this past year, and we can’t wait for all the surprises 2026 will bring! 🤩
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