iiinova — where your digital self keeps evolving

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Victor Tatarskiy @greenrat.bsky.social · 02/10/2025

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

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Mark Copelovitch @mcopelov.bsky.social · 26/09/2025

The first rule of the Phillies And The World Series is that We. Do. Not. Talk. About. The. Phillies. And. The. World. Series. Also, 89% of <Phillies> fans are optimistic? 🤔 #RingTheBell but come on, do it responsibly & honestly 😂

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Damien Fair @drdamienfair.bsky.social · 10/08/2025

I still get chills Meet Mike *30+ years severe depression *first hospitalized @ 13y *20 meds *3 rounds of ECT *2 near-fatal suicide attempts Mike felt joy for the first time in decades after we turned on his new brain pacemaker or PACE see videos, read paper, follow thread doi.org/10.31234/osf...

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Zebrafish Rock! @zebrafishrock.bsky.social · 18/05/2025

Neural activity in the brain when a #zebrafish recognizes food (paramecium). Credit to Dr. Akira Muto. #ZebrafishZunday 🧪

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Jay 🦋 @jay.bsky.team · 10/05/2025

I think about this comic at least once a week

- I want things to be different
- *smashes everything*
- oh no

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Scott Soderling @scottsoderling.bsky.social · 28/04/2025

Over 50 yrs since the discovery of protein kinases, 80% of human kinases still have ≤20 known substrates, and many are “dark.” I'm EXCITED to announce our new work towards solving this- combining (1) deep learning with (2) proximity proteomics in vivo! ➡️ www.biorxiv.org/content/10.1...

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Andrew Octavian Sasmita @aosasmita.bsky.social · 11/03/2025

In-depth investigation of proteomic changes in plaque-associated axonal swellings 😮

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Jenn Cremins @creminslab.bsky.social · 02/03/2025

transcriptionally similar cells can be functionally diverse

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Maxime Beau @maxime-beau.bsky.social · 01/03/2025

I am very proud to announce that my PhD paper finally came out in Cell! In this *very* collaborative study, we develop and release a deep-learning approach to predict neuron type identity from their electrical signature. doi.org/10.1016/j.ce... 1/16 🧵

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Christophe 🔬 Leterrier @christlet.bsky.social · 10/01/2025

Spiny night for #FluorescenceFriday! Trying some out of left field ways to visualize busy samples. Actin (rainbow depth-color coded) and microtubules (gray) along mature dendrites. projection of a 3D-SIM stack with some undisclosed tricks

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Hiten Madhani @hitenmadhani.bsky.social · 07/01/2025

Happy New Year, blue-sci-peeps! My piece in Annual Review of Biophysics on chromatin replication is now available online. doi.org/10.1146/annu... If you have paywall issues, try this link: madhanilab.ucsf.edu/s/annurev-bi...

doi.org

Mechanisms of Inheritance of Chromatin States: From Yeast to Human | Annual Reviews

In this article I review mechanisms that underpin epigenetic inheritance of CpG methylation and histone H3 lysine 9 methylation (H3K9me) in chromatin in fungi and mammals. CpG methylation can be faith...

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Shweta Ramdas @shwetaramdas.bsky.social · 04/01/2025

Our mentor Casey Brown, apart from being an extraordinary scientist, was a brilliant teacher who loved everything genetics. In his honor, some of the world's leading experts came together to create the 'Casey Brown Lecture Series' on human genetics 1/ shorturl.at/eCm7S

shorturl.at

Casey Brown Lecture Series - YouTube

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Jenn Cremins @creminslab.bsky.social · 02/01/2025

crosstalk between mRNA export and transcription

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The Lewis Lab @peterlewislab.bsky.social · 24/12/2024

Epigenetic memory that couples 5mC and H3K9 methylation, enables chromatin state inheritance across lifetimes and evolution. New excellent review by @hitenmadhani.bsky.social highlights inheritance mechanisms including roles for histone chaperones and nucleosome recycling. tinyurl.com/42ddhj4a

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John Anderson 🇨🇦 @janderz8.bsky.social · 20/12/2024

Apparently "functional connectivity" was first used to correlate the activity of individual neurons over time Gerstein, G. L., & Perkel, D. H. (1969). Simultaneously recorded trains of action potentials: analysis and functional interpretation. Science, 164(3881), 828-830.

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Jenn Cremins @creminslab.bsky.social · 19/12/2024

long-term memory without short-term memory

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Jenn Cremins @creminslab.bsky.social · 15/12/2024

Thrilled to see this well-deserved honor for long-time friend and colleague @apombo1.bsky.social - a warm congratulations to Ana and Pombo lab members

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Jenn Cremins @creminslab.bsky.social · 14/12/2024

Congratulations to our alum Dr. Ji Hun Kim who has started his own laboratory at KAIST and just won his first independent grant. Ji Hun is the co-inventor of LADL and will build out the technology to understand condensates in disease. pubmed.ncbi.nlm.nih.gov/31235883/

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Jenn Cremins @creminslab.bsky.social · 14/12/2024

Ten years. Together we built a warm, tight-knit, international community, shared new computational and experimental technologies around the the world, posted >400 preprints, and catalyzed understanding of the mechanisms and functions of chromatin in development and disease.

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Luke Sjulson @lukesjulson.bsky.social · 13/12/2024

Neuroscience students asked us to teach a PRACTICAL course on experimental methods, and it is now on YouTube! Please like and repost to help us get the word out! www.youtube.com/playlist?lis... Lecture 1: Signals and data acquisition Focusing on hardware, digital/analog I/O, synchronization 🧵

youtube.com

Neuroscience methods - YouTube

Nanocourse: Approaches to Study Neural Circuits This course was taught by Anita Autry, Tiago Gonçalves, and Luke Sjulson at Albert Einstein College of Medici...

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Benoit Bruneau @benoitbruneau.bsky.social · 13/12/2024

Best way to cap off 4DN meeting is Karaoke with Bing, @creminslab.bsky.social and many others! So much fun, and most songs sung were Chinese!

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Benoit Bruneau @benoitbruneau.bsky.social · 12/12/2024

San Diego hotpot! With @creminslab.bsky.social and many others. Featuring for this heart guy: aortae!

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Jenn Cremins @creminslab.bsky.social · 12/12/2024

Outstanding talks from the next generation of chromatin researchers - excited for the future @gracebower.bsky.social of @evgenykvon.bsky.social lab @sedonamurphy.bsky.social of Yale Cell Bio Tessa Pompay of Dixon lab Zoe Grant of @benoitbruneau.bsky.social lab Quan Zhu of Shen & Ren labs & others

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Takeshi Imai @imailab.bsky.social · 11/12/2024

From our recent paper: 7-color Tetbow image of the neocortex🌈 We can distinguish hundreds of neurons based on the combinatorial expression of 7 FPs. Here is the list of plasmids for Tetbow experiments if you are interested (all available from Addgene): sites.google.com/site/seedbre...

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Catherine Dulac @dulaclab.bsky.social · 10/12/2024

Antagonistic ties in village social network: an interesting read and 🧵

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Tim Stearns @stearnslab.bsky.social · 08/12/2024

“Acanthocystis turfacea with ingested rotifer” From the beautiful Hudson transparency collection at the University of Exeter. specialcollectionsarchive.exeter.ac.uk/collections/...

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Kristen Panthagani, MD, PhD @kmpanthagani.bsky.social · 14/11/2024

Absolutely fascinating survey from Pew Research on public trust in science came out today. If you are wanting to reach across the aisle and help restore trust in science, the very first step is knowing your audience. Here's what they found. www.pewresearch.org/science/2024...

pewresearch.org

Public Trust in Scientists and Views on Their Role in Policymaking

Americans’ trust in scientists is slightly higher than it was last year, but remains lower than before the COVID-19 pandemic.

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Luca Giorgetti lab @FMI @lucagiorgetti.bsky.social · 06/12/2024

Join us in Banff for the Keystone Interplay of Chromatin Architecture and Transcriptional Regulation meeting next spring (joint with Epigenetics in Development and Disease): www.keystonesymposia.org/conferences/... Short talk abstract coming up - Jan 9

keystonesymposia.org

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Dave Shechner @shechnerlab.bsky.social · 06/12/2024

Hallo! Does anyone have a robust protocol for coupling DNA oligos to proteins? The oligo will be fairly long, and so protocols that require lower mass inputs are preferred. We have free-rein over the oligo chemistry, but the protein is from a commercial source. Feel free to PM. Thanks in advance! 🙏

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rohaniopatel.bsky.social @rohaniopatel.bsky.social · 05/12/2024

Thank you @creminslab.bsky.social!! I am super excited for the work to come, and extremely grateful to the NIH, NHGRI, and the reviewers for believing in my work!

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Molly Przeworski @mollyprz.bsky.social · 05/12/2024

Most of the talks from our Oct meeting are now online, with a few more to come: www.precisionmedicine.columbia.edu/videos

precisionmedicine.columbia.edu

Video Library | Precision Medicine

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Jenn Cremins @creminslab.bsky.social · 05/12/2024

Congratulations to PhD student Rohan Patel @rohaniopatel.bsky.social: Awarded a new NIH F31 fellowship for his work to computationally explore chromatin patterns and mechanisms of loop extrusion in sequential oligopaints imaging data. doi.org/10.1101/2024... x.com/PatelRohanD/...

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Tim Behrens @behrenstimb.bsky.social · 01/12/2024

OK If we are moving to Bluesky I am rescuing my favourite ever twitter thread (Jan 2019). The renamed: Bluesky-sized history of neuroscience (biased by my interests)

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John Ngo @johntngo.bsky.social · 30/11/2024

We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs. You can check out our pre-print here: tinyurl.com/27p7hyt9, and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...

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William J. Brady @williambrady.bsky.social · 28/11/2024

New paper out in @ScienceMagazine! In 8 studies (multiple platforms, methods, time periods) we find: misinformation evokes more outrage than trustworthy news, when it does it's shared more + ppl are less likely to read before sharing. w/ @killianmcl1 @Klonick @mollycrockett 🧵👇

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Luciano A. Masullo @lumasullo.bsky.social · 27/11/2024

ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY. Can we do structural biology with an optical microscope? Yes! Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social [re-post from Twitter]

nature.com

Ångström-resolution fluorescence microscopy - Nature

The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off...

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Jenn Cremins @creminslab.bsky.social · 26/11/2024

Neat thread on perturbing DNA methylation and the real challenges and spinning them into insight. Happy to see deep discussions about science take center stage. Kudos @maxvcg.bsky.social and team

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Christophe 🔬 Leterrier @christlet.bsky.social · 25/11/2024

A complement to @peiferlabunc.bsky.social reposting this image – it was the cover of the 40th anniversary issue of J Neuroscience back in 2021, together with an historical review on imaging the neuronal cytoskeleton with lots of cool pictures: www.jneurosci.org/content/41/1...

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Jenn Cremins @creminslab.bsky.social · 25/11/2024

Congratulations Dr. Chandrashekar 💐 Using human neurons homozygously-engineered with familial AD mutations, Harshini found a role for miswired promoter-enhancer multi-loops in dysregulated excitatory and inhibitory gene expression programs doi.org/10.1101/2024...

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Jenn Cremins @creminslab.bsky.social · 24/11/2024

Interneuron subtypes shift their composition with changes in pyramidal neuron prevalence. SST interneurons adjust abundance through programmed cell death - and PV interneurons shape shift their identity. Cool, from @gordfishell and team.

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