Standard algorithms fail to fully exploit the spatial information in DNA-PAINT. Here, the authors present G5M, an algorithm providing molecular maps by accurately inferring biomolecule positions at di...

4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

Light: Science & Applications - Localization of single molecules with structured illumination and structured detection

By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

The nanoscale organization of the antigen-antibody complexes influences the therapeutic action of monoclonal antibodies. Here, the authors present a multi-target 3D RESI imaging assay for the nanomete...

Zaza and colleagues demonstrate that DNA-PAINT on a spinning disk confocal microscope with optical photon reassignment enables high-resolution imaging across large fields and imaging depths, resolving...

Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch. - HarrisonKramer/optiland

STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

Mammalian cells present RNA-binding proteins on the cell surface that form clustered domains containing glycoRNAs.

Over the last 30 years, fluorescence microscopy, renowned for its sensitivity and specificity, has undergone a revolution in resolving ever-smaller details. This advancement began with stimulated emis...

The ability to rapidly image mesoscopic samples is critically important for many areas of biological research. Owing to its high throughput and gentle, volumetric imaging nature, light-sheet fluoresce...

Various super-resolution fluorescence microscopy methods achieve nanoscale resolution, vital for visualizing subcellular structures. Choosing suitable biological standards is challenging, demanding p...

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The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off...