iiinova — where your digital self keeps evolving

Jenn Cremins @creminslab.bsky.social · 31/07/2026

Congratulations! Loved learning about your work

Reposted by Jenn Cremins

Victor Tatarskiy @greenrat.bsky.social · 02/10/2025

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

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Mark Copelovitch @mcopelov.bsky.social · 26/09/2025

The first rule of the Phillies And The World Series is that We. Do. Not. Talk. About. The. Phillies. And. The. World. Series. Also, 89% of <Phillies> fans are optimistic? 🤔 #RingTheBell but come on, do it responsibly & honestly 😂

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Reposted by Jenn Cremins

Damien Fair @drdamienfair.bsky.social · 10/08/2025

I still get chills Meet Mike *30+ years severe depression *first hospitalized @ 13y *20 meds *3 rounds of ECT *2 near-fatal suicide attempts Mike felt joy for the first time in decades after we turned on his new brain pacemaker or PACE see videos, read paper, follow thread doi.org/10.31234/osf...

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Jenn Cremins @creminslab.bsky.social · 08/06/2025

Neat Elphege. Look forward to seeing the data play out and if there are curious functional differences for any chromatin modification in post-mitotic vs. dividing cell states

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Jenn Cremins @creminslab.bsky.social · 08/06/2025

My pleasure LPS-stimulated macrophages pmc.ncbi.nlm.nih.gov/articles/PMC... Neural maturation in vivo and pharmacologically stimulated post-mitotic neurons in vitro elifesciences.org/articles/76539 human post-mitotic neurons www.biorxiv.org/content/10.1... as they say: Mo' Loops, Mo' Problems

elifesciences.org

Cohesin-dependence of neuronal gene expression relates to chromatin loop length

Cohesin is required for the expression of neuronal genes that engage in long chromatin loops, while short-range enhancer-promoter loops can form in the absence of cohesin.

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Jenn Cremins @creminslab.bsky.social · 03/06/2025

-Critical developmentally regulated enhancer-promoter loops -Establishment of new gene expression, not maintenance -Genes with multiple distant enhancers -In physiologic or developmentally relevant models -Strong perturbations and rigorous technology -Disruption linked to severe disease phenotypes

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Jenn Cremins @creminslab.bsky.social · 03/06/2025

It has been unambiguously demonstrated that loops are necessary for the establishment of new induced gene expression programs in post-mitotic neurons in vitro and in vivo and in macrophages in vitro. Clean, strong perturbations in physiolgically or developmentally relevant models helped resolve.

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Jenn Cremins @creminslab.bsky.social · 29/05/2025

It's profound how the OP insight scales to life in science too. The scientists that find that absolutely most amazing creative unexpected patterns in data that are actually true and stand the test of time tend to obsess about every minor detail and dot every i and cross every t.

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Reposted by Jenn Cremins

Zebrafish Rock! 🎃 @zebrafishrock.bsky.social · 18/05/2025

Neural activity in the brain when a #zebrafish recognizes food (paramecium). Credit to Dr. Akira Muto. #ZebrafishZunday 🧪

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Jenn Cremins @creminslab.bsky.social · 16/05/2025

It's beyond hard right now - profound disappointment in the system. Yet - science and the power of the scientific method to seek truth is timeless. Truth always comes into the light in the end. Your skills and all you've trained to learn will matter more than ever in the coming years.

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Jenn Cremins @creminslab.bsky.social · 16/05/2025

100% in your corner and in the corner of your very bright future no matter what comes next

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Reposted by Jenn Cremins

Jay 🦋 @jay.bsky.team · 10/05/2025

I think about this comic at least once a week

- I want things to be different
- *smashes everything*
- oh no

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Reposted by Jenn Cremins

Scott Soderling @scottsoderling.bsky.social · 28/04/2025

Over 50 yrs since the discovery of protein kinases, 80% of human kinases still have ≤20 known substrates, and many are “dark.” I'm EXCITED to announce our new work towards solving this- combining (1) deep learning with (2) proximity proteomics in vivo! ➡️ www.biorxiv.org/content/10.1...

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Jenn Cremins @creminslab.bsky.social · 27/04/2025

awesome family - and Oracle from the inside 🤩

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Jenn Cremins @creminslab.bsky.social · 27/04/2025

congratulations to Soderling III on carrying the family legacy!

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Reposted by Jenn Cremins

Andrew Octavian Sasmita @aosasmita.bsky.social · 11/03/2025

In-depth investigation of proteomic changes in plaque-associated axonal swellings 😮

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Jenn Cremins @creminslab.bsky.social · 09/03/2025

Empowerment really is as simple as this.

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Jenn Cremins @creminslab.bsky.social · 02/03/2025

transcriptionally similar cells can be functionally diverse

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Reposted by Jenn Cremins

Maxime Beau @maxime-beau.bsky.social · 01/03/2025

I am very proud to announce that my PhD paper finally came out in Cell! In this *very* collaborative study, we develop and release a deep-learning approach to predict neuron type identity from their electrical signature. doi.org/10.1016/j.ce... 1/16 🧵

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Reposted by Jenn Cremins

Christophe 🔬 Leterrier @christlet.bsky.social · 10/01/2025

Spiny night for #FluorescenceFriday! Trying some out of left field ways to visualize busy samples. Actin (rainbow depth-color coded) and microtubules (gray) along mature dendrites. projection of a 3D-SIM stack with some undisclosed tricks

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Reposted by Jenn Cremins

Hiten Madhani @hitenmadhani.bsky.social · 07/01/2025

Happy New Year, blue-sci-peeps! My piece in Annual Review of Biophysics on chromatin replication is now available online. doi.org/10.1146/annu... If you have paywall issues, try this link: madhanilab.ucsf.edu/s/annurev-bi...

doi.org

Mechanisms of Inheritance of Chromatin States: From Yeast to Human | Annual Reviews

In this article I review mechanisms that underpin epigenetic inheritance of CpG methylation and histone H3 lysine 9 methylation (H3K9me) in chromatin in fungi and mammals. CpG methylation can be faith...

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Jenn Cremins @creminslab.bsky.social · 06/01/2025

One question I've been pondering is how responsiveness to the NOSI is evaluated in study section. If one takes great care to design a grant to respond to the NOSI - and the grant goes to a standard study section - does the SRO update the reviewers on special things to look for to evaluate?

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Reposted by Jenn Cremins

Shweta Ramdas @shwetaramdas.bsky.social · 04/01/2025

Our mentor Casey Brown, apart from being an extraordinary scientist, was a brilliant teacher who loved everything genetics. In his honor, some of the world's leading experts came together to create the 'Casey Brown Lecture Series' on human genetics 1/ shorturl.at/eCm7S

shorturl.at

Casey Brown Lecture Series - YouTube

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Jenn Cremins @creminslab.bsky.social · 02/01/2025

crosstalk between mRNA export and transcription

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Reposted by Jenn Cremins

The Lewis Lab @peterlewislab.bsky.social · 24/12/2024

Epigenetic memory that couples 5mC and H3K9 methylation, enables chromatin state inheritance across lifetimes and evolution. New excellent review by @hitenmadhani.bsky.social highlights inheritance mechanisms including roles for histone chaperones and nucleosome recycling. tinyurl.com/42ddhj4a

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Jenn Cremins @creminslab.bsky.social · 23/12/2024

Technotriumphalism: a force in fields of chromatin and gene regulation too. My optimism is that it appears the winds of change might bring back the value of elegance and depth of conceptual thought. And the beauty in historic literature.

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Reposted by Jenn Cremins

John Anderson 🇨🇦 @janderz8.bsky.social · 20/12/2024

Apparently "functional connectivity" was first used to correlate the activity of individual neurons over time Gerstein, G. L., & Perkel, D. H. (1969). Simultaneously recorded trains of action potentials: analysis and functional interpretation. Science, 164(3881), 828-830.

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Jenn Cremins @creminslab.bsky.social · 20/12/2024

we celebrated him and 25 years of his impact on all of us in June - you can see the trigger words if you look closely at his garish blue slides from the roast

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Jenn Cremins @creminslab.bsky.social · 19/12/2024

Never a burden. Grief is real, raw, authentic and a shared experience of all of humanity. Without grief, we have no love, and then life's simply not worth it at all.

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Jenn Cremins @creminslab.bsky.social · 19/12/2024

long-term memory without short-term memory

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Jenn Cremins @creminslab.bsky.social · 17/12/2024

happy to be here - see you tomorrow!

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Jenn Cremins @creminslab.bsky.social · 15/12/2024

Thrilled to see this well-deserved honor for long-time friend and colleague @apombo1.bsky.social - a warm congratulations to Ana and Pombo lab members

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Jenn Cremins @creminslab.bsky.social · 14/12/2024

Thank you to many wonderful colleagues who invested in our community and allowed all voices to be valued and heard. Our perspective mattered, we belonged, and it made the difference for the quality and impact of the science. Tangible excitement for the future - the best is yet to come. Onward.

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Jenn Cremins @creminslab.bsky.social · 14/12/2024

Congratulations to our alum Dr. Ji Hun Kim who has started his own laboratory at KAIST and just won his first independent grant. Ji Hun is the co-inventor of LADL and will build out the technology to understand condensates in disease. pubmed.ncbi.nlm.nih.gov/31235883/

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Jenn Cremins @creminslab.bsky.social · 14/12/2024

Thank you to the NIH and all of the 4DNucleome common fund staff for all they have done to support us, inspire us, and to bring us together. We will need each other more than ever in the coming months as we stand for the future of basic science research.

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Jenn Cremins @creminslab.bsky.social · 14/12/2024

Ten years. Together we built a warm, tight-knit, international community, shared new computational and experimental technologies around the the world, posted >400 preprints, and catalyzed understanding of the mechanisms and functions of chromatin in development and disease.

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Luke Sjulson @lukesjulson.bsky.social · 13/12/2024

Neuroscience students asked us to teach a PRACTICAL course on experimental methods, and it is now on YouTube! Please like and repost to help us get the word out! www.youtube.com/playlist?lis... Lecture 1: Signals and data acquisition Focusing on hardware, digital/analog I/O, synchronization 🧵