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woj_wie

@wojwie.bsky.social
822 followers 69 following 9 posts

Photosynthesis and cryoEM at Biozentrum, Basel.

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Manon Demulder @manondemulder.bsky.social · 02/10/2026
If you’ve always been curious about how algae fix carbon, @phaips.vd.st, @cellarchlab.com and I have put together the quickest route into pyrenoids. And if you don’t feel like reading at all, we have an accompanying poster doi.org/10.1242/jcs....
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Caitie McCafferty @computingcaitie.bsky.social · 09/09/2026
I am looking for a lab manager for my group that I will be starting at the @fmiscience.bsky.social in February! We will use in situ structural biology to investigate dynamic ciliary processes. More info here —> bsky.app/profile/comp... #cilia #teamtomo #proteomics #protistsky
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Florent Waltz @florentwaltz.bsky.social · 07/08/2026
We've been seeing a lot of "in situ" lately in titles from fancy journals (not pointing any fingers 👀). I’ve delayed posting this, but as more and more come out, I'm afraid "in situ" is becoming an empty buzzword, much like "near-atomic" was for SPA 🔬 1/n #cryo-ET #cryo-EM #cryoET #cryoEM 🧪
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Lorna Malone @drlornamalone.bsky.social · 13/07/2026
⏰ 6 days left to apply for an in-person spot on the Wellcome–MRC in situ Cryo-ET Workshop 2026! 📅 28 Sept – 2 Oct '26 🔬 #Cryo-ET & #Cryo-FIB lectures, demos & practicals 📍 @diamondlightsource.bsky.social & @astburycentre.bsky.social 💻 Lectures also available on Zoom Apply now: shorturl.at/LRihg
shorturl.at
Wellcome-MRC in situ Cryo-ET Workshop 2026 - - Diamond Light Source
eBIC (Diamond light source) has partnered with the Astbury Biostructure Lab (University of Leeds), the Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Fac...
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woj_wie @wojwie.bsky.social · 22/06/2026
Caitie’s a lion 💪♌️🦁🐥. Go work with her to do and learn cool stuff. I’d consider myself… but photosynthesis > cilia 😉@sofie-dot-rec.bsky.social . Congrats Caitie!
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Manon Demulder @manondemulder.bsky.social · 21/05/2026
Our next DinoSphere seminar will be June 18th at 9 AM PDT / 6 PM CEST hosting Jana Pilátová and @gracezhong.bsky.social 🪱 Grace Zhong: Symbiont mobility in a dinoflagellate–acoel worm symbiosis 🪩 Jana Pilátová: Eyespots as photonic mirrors in Symbiodiniaceae More details on tinyurl.com/43uckr2w!
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Caitie McCafferty @computingcaitie.bsky.social · 24/05/2026
Great opportunity to work in an exciting environment and a wonderful group!!
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Lorna Malone @drlornamalone.bsky.social · 22/05/2026
🎥 Kicking off now: the eBIC Cryo Lift-Out webinar @diamondlightsource.bsky.social Join us to learn about new cryo lift-out workflows for vitrified tissues and small organisms using cryo-ET, plus a live Q&A with the team 🕚 Happening now! 🔗 bit.ly/cryo-Lift-Out 🔐 #CryoEM #CryoET
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Join our Cloud HD Video Meeting
Zoom is the leader in modern enterprise cloud communications.
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woj_wie @wojwie.bsky.social · 12/03/2026
Interesting and important research, with someone that will care for you. Recommended. Allez Manon!
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Manon Demulder @manondemulder.bsky.social · 11/03/2026
Excited to announce I’m starting a new lab at @gmivienna.bsky.social (Vienna BioCentre) in Sept 2026 We study how carbon fixation is inherited and maintained in algae using cryo-ET, imaging, environmental sampling&more Looking for people who want to build the lab together, get in touch! #teamtomo
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Gregor Mendel Institute of Molecular Plant Biology @gmivienna.bsky.social · 04/03/2026
Apply now! @vbcscitraining.bsky.social offers more than 25 fully-funded PhD positions! GMI group leaders are also recruiting new students.
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Gregor Weiss @weiss-lab.bsky.social · 19/01/2026
🔬 Postdoc position – Infection Biology & Cryo-ET (Zurich) 🇨🇭 We are looking for a motivated Postdoc to join our interdisciplinary team. 📩 Interested? Check out the job advert: www.imm.uzh.ch/dam/jcr:cb17... 👉 More info on the lab: www.weiss-laboratory.com Please share with interested colleagues!
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Vienna BioCenter Scientific Training @vbcscitraining.bsky.social · 05/01/2026
The Vienna BioCenter Summer School 2026 call is open for talented undergrads, it's a great opportunity for students who are interested in graduate study in the life sciences. Sven Klumpe from the IMP/IMBA is recruiting! training.vbc.ac.at/summer-school
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Katharina Geißler @kgeissler.bsky.social · 16/12/2025
It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
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Matt Johnson @profmattjohnson.bsky.social · 12/11/2025
Our new preprint 🚨🌱☀️on flourescent biosensing of ATP levels in chloroplasts in vivo shows disequilibrium between proton motive force and ATP levels. We show PGR5 dependent cyclic electron transfer does not augment ATP/NADPH ratios @gustaf23.bsky.social 🙌 www.biorxiv.org/cgi/content/...
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woj_wie @wojwie.bsky.social · 07/11/2025
It’s strange. I know it’s just an option and not the first AI-assist. but imo the main positive sounds like endorsing scientist to trust opinions of a company(?)-run blackbox. A posterior rather than during design. And it’s coming from the place that to many was the first step to improve publishing…
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Manon Demulder @manondemulder.bsky.social · 28/10/2025
We’re back with the next DinoSphere Online Seminar! Join us on Nov 4th, we're hosting: Edmée Royen (ULiège) - Symbiodinium
 Nicolas dos Santos Pacheco (Cambridge) - Perkinsids 📅 Tue, Nov 4 - 4 PM CET/3 PM GMT 
💻 Zoom link: sites.google.com/view/dinosphere
 Please spread the word! #protistonsky
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Max Planck Institute of Biophysics @mpibp.bsky.social · 23/10/2025
Have you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? 🧊 Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts! 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-... 📍 Frankfurt | 📆 April 12–20, 2026
Orange and green poster of the In-situ structural biology by Cryo-FIB and Cryo-ET in Frankfurt am Main, with EMBO logo. The poster promotes childcare grants, travel grants and that this is a sustainable event.
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Vanni Lab at UNIFR, Switzerland @labvanni.bsky.social · 13/10/2025
Thanks to @snsf.ch, we are hiring two PhD students to investigate frontier topics in lipid biology using a combination of MD simulations and experiments. Preferred starting date: ASAP. Fribourg - and Switzerland - are quite nice, for both work and private life! Please spread the word!
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Biozentrum, University of Basel @biozentrum.unibas.ch · 29/09/2025
Assistant/Associate Professor in Structural Biology, Biophysics or Biological Imaging www.biozentrum.unibas.ch/open-positio... #biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging
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Ben Engel @cellarchlab.com · 25/09/2025
Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on! #TeamTomo #PlantScience 🧪 🧶🧬 🌾 elifesciences.org/articles/105... 1/🧵
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Sven Klumpe @svenklumpe.bsky.social · 15/08/2025
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
meetings.embo.org
In situ structural biology by Cryo-FIB and Cryo-ET
Cryo-focused ion beam/scanning electron microscopy (cryo-FIB/SEM) and cryo-electron tomography (cryo-ET) are emerging, versatile tools for structural cell biologists, aiming at the exploration of mol…
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Biozentrum, University of Basel @biozentrum.unibas.ch · 04/08/2025
Apply now for the prestigious and independent #BiozentrumPhDFellowships. Great science. Unique rotation-based selection of research group and other incentives. The summer call is open until October 12, 2025. bit.ly/4caiqqX @biozentrum.unibas.ch @unibas.ch #fellowship #PhD#Switzerland
bit.ly
Biozentrum PhD Fellowships
Share your passion for life sciences. If you are talented and highly motivated, want to broaden your horizons and are interested in a wide range of research topics, apply for one of the sought after B...
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Manon Demulder @manondemulder.bsky.social · 30/06/2025
Our first DinoSphere seminar is this Tuesday, 1 July at 9 AM CEST! A good way to start the day if you're in Europe ☕ We will hear from Yong Heng Phua (OIST) and Karel Mocaer (Heidelberg) 🔗 Zoom link on our website: tinyurl.com/4duvwtvp
tinyurl.com
DinoSphere - Online talks
Video credits: Liz Cooney
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Florian Wollweber @florianwollweber.bsky.social · 27/06/2025
After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis First job ad: #teamtomo scientist!🔬❄️ tinyurl.com/2rdu2ze6
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Manon Demulder @manondemulder.bsky.social · 04/06/2025
We’re kicking off the DinoSphere Online Seminar Series! Join us for our first session with Karel Mockaer (Heidelberg) & Yong Heng Phua (OIST) 📅 1 July 9AM CEST 🔗 tinyurl.com/4mjaverj Spread the word! @protistwtmostest.bsky.social @ehehenberger.bsky.social @chandnibhickta.bsky.social&Norico Yamada
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Matt Johnson @profmattjohnson.bsky.social · 21/05/2025
Pleased to share my very general review of Photosynthesis now published in Nature Reviews Mol Cell Biol 🌞🌱 rdcu.be/em1lz Hopefully a useful primer for Postgrad and undergrad students
rdcu.be
Structure, regulation and assembly of the photosynthetic electron transport chain
Nature Reviews Molecular Cell Biology - The electron transfer chain in chloroplast thylakoid membranes uses solar energy to produce NADPH and ATP, which power carbon fixation into biomass. This...
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George Currie @georgealfredcurrie.bsky.social · 19/05/2025
Does publishing serve science or is science serving publishing? Damian Pattinson and I (@elife.bsky.social) argue scientific publishing has evolved into a system that, rather than facilitate scholarly communication, distorts and dictates it. onlinelibrary.wiley.com/doi/10.1002/... #OpenScience
Screenshot of article summary of: Toward Science-Led Publishing
by Damian Pattinson, George Currie

published as an opinion piece, in Learned Publishing

Summary

The current dynamic of scholarly publishing prioritises the wants of the publishing industry over the needs of the research community.

This article explores this theme through the lens of ‘publisher-led science’ as a description of our current status quo, and through ‘science-led publishing’ as an improved future state.

We argue that financial motivations central to most publishing distort how research is presented, how it is assessed and even what research is undertaken, leading to a system that hinders, rather than facilitates, scientific progress.

We propose three elements of a science-led publishing approach that would accelerate research communication, incentivise collaboration between authors, editors and reviewers, and create a more transparent and equitable research landscape.

We believe that research funding and research assessment are two of the primary levers for wider change in research and research culture and consider the future purpose of scholarly publishing in a world where these proposals have been widely adopted.
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Philippe Van der Stappen @phaips.vd.st · 06/05/2025
Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪
tomoguide.github.io
Welcome to TomoGuide
A step-by-step Cryo-ET guide
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Florent Waltz @florentwaltz.bsky.social · 06/05/2025
You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !
3.8 angstrom resolution ribosome from 33 tomograms The TomoGuide website
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Lorenz Lamm @lorenzlamm.bsky.social · 25/04/2025
🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo
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Philipp Erdmann @3p1l.bsky.social · 22/04/2025
Hey Everyone!❄️🔬 We have two fully funded PhD positions @humantechnopole.bsky.social this year! Help us understand better how one of the main stress regulators - the nucleolus - integrates synaptic inputs!🧠Find all the calls - also from my amazing colleagues - here: www.semm.it/education/ho...
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Florian Wollweber @florianwollweber.bsky.social · 21/03/2025
Asgard archaea are full of surprises, this time we found (tiny) microtubules (snapshots of microtubule evolution?!) - Check out our new paper on the cytoskeleton of Lokiarchaeum ossiferum: www.cell.com/cell/fulltex...
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Florent Waltz @florentwaltz.bsky.social · 20/03/2025
The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨
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Katharina Geißler @kgeissler.bsky.social · 15/03/2025
Check out @deglushk.bsky.social 's method to measure membrane thickness in cryo electron tomograms!
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Richard Sever @richardsever.bsky.social · 11/03/2025
Big news: we are setting up a new non-profit organization to run bioRxiv and medRxiv. It's called openRxiv [no it's not a new preprint server; it's dedicated organization to oversee the servers] openrxiv.org 1/n
openrxiv.org
Homepage - openRxiv
openRxiv is an independent non-profit, the new organizational home for bioRxiv and medRxiv, enabling researchers to instantly share groundbreaking findings with the global scientific community.
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Carl T. Bergstrom @carlbergstrom.com · 06/03/2025
Let's talk about this Nature piece in more detail. I've rarely read something so anti-scientific anywhere short of the National Review. www.nature.com/articles/d41...
nature.com
Three AI-powered steps to faster, smarter peer review
Tired of spending countless hours on peer reviews? An AI-assisted workflow could help.
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Sven Klumpe @svenklumpe.bsky.social · 05/03/2025
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
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Ricardo D. Righetto @lifeonthewedge.bsky.social · 28/02/2025
To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻 www.ebi.ac.uk/empiar/EMPIA... A little thread about what's new... 🧵 1/n
Rare cellular events observed in the cryo-ET dataset. Slices through tomograms (left) with corresponding 3D segmentation (middle and right). Right panels show enlarged views of the boxed regions in the middle panels. A) Mitochondrion at the plasma membrane, tethered to microtubules by thin filaments of unknown identity. Segmented classes: nuclear envelope (light blue), nuclear pore complex (dark blue), 80S ribosomes (orange), mitochondrial membranes (dark red), ATP synthase (royal blue), plasma membranes (grey), microtubules (pink), filaments (yellow), endoplasmic reticulum (purple). Zoomed-in panel shows thin filaments running between microtubules and the mitochondrion at the plasma membrane. Note that this tomogram contains two closely appressed cells, and thus, two plasma membranes with cell walls between. B) Mitochondrial fission event with ER membrane interactions. Segmented classes: mitochondrial membranes (dark red), ATP synthase (royal blue), 80S ribosomes (orange), thylakoid membranes (green), PSII (bright yellow), cell wall (grey), ER (purple), fission site containing filamentous structures perpendicular to the mitochondrial long axis (pale yellow). C) Ciliary transition zone between basal body and axoneme, including assembling IFT train and stellate structure77. Segmented classes: microtubule doublets (light blue), central microtubule pair (light green), stellate structure (pale red), IFT train (yellow). D) Pyrenoid tubule extending from the thylakoids into the phase-separated Rubisco matrix of the pyrenoid. Minitubules originate from thylakoid membranes82. Segmented classes: thylakoid membranes (dark green), PSII (yellow), pyrenoid tubule (lime green), Rubisco (lavender blue), minitubules (orange). Scale bars in A-D: 100 nm. Related to Fig. 2C-H.
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Lorna Malone @drlornamalone.bsky.social · 18/02/2025
📢 Registration is now open for 'Wellcome-MRC Cryo-EM in Structural Biology 2025'! 📅 Join us online March 3-7 at @diamondlightsource.bsky.social @universityofleeds.bsky.social for lectures & demos in #CryoEM & #CryoET: sample prep, SPA, model building & more!🧬 🔗 www.diamond.ac.uk/Instruments/...
diamond.ac.uk
Wellcome-MRC Cryogenic Electron Microscopy in Structural Biology 2025 - - Diamond Light Source
eBIC (Diamond light source) has partnered with the Astbury Biostructure Lab (University of Leeds), the Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Fac...
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Marcel Dickmanns | 🌿❄️🔬 @madic.bsky.social · 19/02/2025
Our methods paper made it to bioRxiv: doi.org/10.1101/2025... Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5 #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM
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Sven Klumpe @svenklumpe.bsky.social · 04/02/2025
Extremely excited to be joining the vibrant community of the Vienna BioCenter to build up cryo-ET and cryo-FIB milling on campus soon!
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Sjors Scheres @sjorsscheres.bsky.social · 04/02/2025
Give control to shareholders, and enshittification will happen, where a relentless drive for growth will reduce quality for users. It would be cheaper and better to keep companies away from control over scientific publishing. Scientists do most of the work anyway. Look at @elife.bsky.social!
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Bianca Introini @bianca-introini.bsky.social · 27/01/2025
I am very happy to share our latest work on the detailed cryo-EM structure of the NDH-PSI supercomplex of spinach. Thanks to Werner Kühlbrandt, Alex Hahn and @mpibp.bsky.social ! 🍃❄️🔬 You can find the paper in open access in Nature Structural and Molecular Biology: www.nature.com/articles/s41...
nature.com
Cryo-EM structure of the NDH–PSI–LHCI supercomplex from Spinacia oleracea - Nature Structural & Molecular Biology
Here, the authors report the structure of the nicotinamide adenine dinucleotide phosphate dehydrogenase–photosystem I–light-harvesting complex I supercomplex, providing insights into subunit interacti...
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Yannick Schwab @yschwab.bsky.social · 24/01/2025
Another reminder, we are soon reaching the max number of participants so now is a good time to register #volumeEM
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SBGrid Consortium @sbgrid.bsky.social · 23/01/2025
In case you missed our first webinar of 2025, it is now available. Lorenz Lamm from MUDS - Munich School for Data Science presented MemBrainv2- an end-to-end tool for the analysis of membranes in cryo-electron tomography. buff.ly/4hutFwf #SBGridwebinars #ScienceMatters
buff.ly
MemBrainv2
SBGrid webinars are hosted with partial support from the NIH R25 Continuing Education for Structural Biology Mentors #GM151273, in collaboration with Co-PI J...
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Richard Sever @richardsever.bsky.social · 18/01/2025
Announcing the new bioRxiv dashboard widget - it's now easier to when articles have comments, peer reviews, community discussion, media coverage, videos, and automated analyses 1/2 connect.biorxiv.org/news/2025/01...
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Priya Ramakrishna @priyaramakrishna.bsky.social · 16/01/2025
Most plants can`t handle salt, but have evolved strategies to survive in it. How do they do it?🌱🧪 Excited to share some of our first insights using a new cryoelemental imaging technique to answer these questions at the subcellular scale!(1/8) www.nature.com/articles/s41... @nature.com #PlantScience
nature.com
Elemental cryo-imaging reveals SOS1-dependent vacuolar sodium accumulation - Nature
This study demonstrates that cryo nanoscale secondary ion mass spectrometry (CryoNanoSIMS) enables direct multi-elemental imaging at subcellular resolution of macro- and micronutrients or trace elemen...
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Lorenz Lamm @lorenzlamm.bsky.social · 13/01/2025
Looking forward to talking about MemBrain-seg tomorrow and giving a sneak peek into other MemBrain v2 functionalities 👀🚀
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Max Planck Institute of Biophysics @mpibp.bsky.social · 07/01/2025
At the intersection of art and science 🎨🧪 some masterpieces reveal fascinating lessons in #biophysics! Join Prof. Werner Kühlbrandt on a unique tour of the Städel Museum, where he explores mirror symmetry, cellular energy, and more. Read more: www.biophys.mpg.de/2840124/art-... 🔗 #ArtMeetsScience
Prof. Werner Kühlbrandt stands before the iconic Goethe in the Roman Campagna painting at the Städel Museum in Frankfurt. c Shau Chung Shin.
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