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Matt Johnson

@profmattjohnson.bsky.social
624 followers 1.1K following 25 posts

Group leader at University of Sheffield, photosynthesis 🌱☀️, plant physiology, biochemistry and structural biology 🔬🇪🇺🇬🇧 www.Sheffield.ac.uk/photosynthesis

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Matt Johnson @profmattjohnson.bsky.social · 06/09/2026
Great to share our new preprint on Chlamy’s chloroplast ATP synthase at 2.2 Å we see 13 c subunits, a modified redox regulation and a complete Grotthuss proton wire 🦠☀️ www.biorxiv.org/content/10.6...
biorxiv.org
A 13-subunit c-ring in the Chlamydomonas chloroplast ATP synthase lowers the H⁺/ATP cost of carbon fixation
The chloroplast F1Fo ATP synthase is a rotary motor that converts the light-driven proton-motive force into the chemical energy of ATP. The number of c-subunits in its rotor fixes the number of proton...
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Gustaf Degen @gustaf23.bsky.social · 17/08/2026
Please to share our paper in @natplants.nature.com showing that PGR5 is not required for ATP augmentation in Arabidopsis chloroplasts @profmattjohnson.bsky.social @schwarzlanderlab.bsky.social www.nature.com/articles/s41...
nature.com
Disequilibrium between chloroplast proton motive force and ATP levels in Arabidopsis - Nature Plants
A fluorescent ATP biosensor reveals that chloroplast ATP levels remain unchanged in Arabidopsis mutants with a much weaker proton gradient, showing that cyclic electron transport protects photosynthes...
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Plant Energy Biology Lab @schwarzlanderlab.bsky.social · 17/08/2026
50% PMF🪫keeps ATP at 100%🔋 www.nature.com/articles/s41... @natplants.nature.com CET provides ∆pH for photoprotection, while the redox balance is most likely handled by the malate valve 🍃☀️ Based on elegant in vivo ATP biosensing by @gustaf23.bsky.social & @profmattjohnson.bsky.social
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Matt Johnson @profmattjohnson.bsky.social · 17/08/2026
For 20 yrs textbook view: linear electron flow can't make enough ATP for CO₂ fixation, so plants use cyclic electron transfer (CET) to top it up. Today in @natplants.nature.com we measured ATP directly inside living chloroplasts — and that story doesn't hold. www.nature.com/articles/s41...
nature.com
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Jamie Blaza @jnb-lab.bsky.social · 10/08/2026
Permanent academic position as a Lecturer in cryo-ET open at York. We are looking for someone to lead a research program making the most of the stunning advances occurring in cryo-ET. It's a research and teaching post but with a focus on research in the early years. jobs.york.ac.uk/vacancy/lect...
jobs.york.ac.uk
Jobs - The University of York
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Matt Johnson @profmattjohnson.bsky.social · 09/08/2026
Why supercomplexes? 🌱☀️🦠 🚨New 3 year Postdoc opportunity 🚨 A BBSRC sponsored project using single molecule AFM to interrogate ephemeral interactions between ET proteins in respiration and photosynthesis Join our diverse team and Apply here: sheffield.ac.uk/photosynthes...
sheffield.ac.uk
BBSRC Sponsored Postdoc Position
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Gustaf Degen @gustaf23.bsky.social · 06/06/2026
Pleased to share our preprint on “Loss of PSAE redirects PGRL1 to photosystem I and enhances PGR5-dependent cyclic electron transfer in Arabidopsis” @profmattjohnson.bsky.social @sheffieldpps.bsky.social www.biorxiv.org/content/10.6...
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Matt Johnson @profmattjohnson.bsky.social · 12/11/2025
Our new preprint 🚨🌱☀️on flourescent biosensing of ATP levels in chloroplasts in vivo shows disequilibrium between proton motive force and ATP levels. We show PGR5 dependent cyclic electron transfer does not augment ATP/NADPH ratios @gustaf23.bsky.social 🙌 www.biorxiv.org/cgi/content/...
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Ben Engel @cellarchlab.com · 25/09/2025
Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on! #TeamTomo #PlantScience 🧪 🧶🧬 🌾 elifesciences.org/articles/105... 1/🧵
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Matt Johnson @profmattjohnson.bsky.social · 21/05/2025
Pleased to share my very general review of Photosynthesis now published in Nature Reviews Mol Cell Biol 🌞🌱 rdcu.be/em1lz Hopefully a useful primer for Postgrad and undergrad students
rdcu.be
Structure, regulation and assembly of the photosynthetic electron transport chain
Nature Reviews Molecular Cell Biology - The electron transfer chain in chloroplast thylakoid membranes uses solar energy to produce NADPH and ATP, which power carbon fixation into biomass. This...
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Plants, Photosynthesis and Soil @sheffieldpps.bsky.social · 08/05/2025
2. Towards climate-change resilient crops: unravelling relationships between CO2 assimilation and ATP production led by @profmattjohnson.bsky.social Power photosynthesis for climate-smart crops. Investigate CO₂ uptake + ATP production. bit.ly/4m5BZ8X #PlantScience #AcademicSky
bit.ly
Towards climate-change resilient crops by unravelling the relationship between CO2 assimilation and ATP production at University of Sheffield on FindAPhD.com
PhD Project - Towards climate-change resilient crops by unravelling the relationship between CO2 assimilation and ATP production at University of Sheffield, listed on FindAPhD.com
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Gustaf Degen @gustaf23.bsky.social · 03/03/2025
Finally! After a lot of hard work our paper on tethering FNR to PSI in Chlamy is out in @theplantcell.bsky.social @sheffieldpps.bsky.social @profmattjohnson.bsky.social academic.oup.com/plcell/advan...
academic.oup.com
Tethering ferredoxin-NADP+ reductase to photosystem I promotes photosynthetic cyclic electron transfer
The location of the key photosynthetic enzyme FNR impacts the partitioning of photosynthetic electrons between the linear and cyclic electron transport pat
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Ryo Yokoyama @yokoyama-ryo.bsky.social · 19/12/2024
A family of NADPH/NADP+ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes www.nature.com/articles/s41... @naturecomms.bsky.social
nature.com
A family of NADPH/NADP+ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes - Nature Communications
This article presents NAPstars, a family of genetically-encoded biosensors that enable real-time monitoring of NADP redox dynamics across species. The sensors reveal robust NADP redox regulation, cell...
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Plants, Photosynthesis and Soil @sheffieldpps.bsky.social · 29/11/2024
Determining role of FNR location in photoprotection of photosystem I buff.ly/4imFBBM Life depends on photosynthesis; you will study a key photoprotective regulatory mechanism controlling electrical current flow between photosystems matt.johnson@sheffield.ac.uk @profmattjohnson.bsky.social
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woj_wie @wojwie.bsky.social · 27/11/2024
This one was delayed for a while… apologies to the musicians. Collab with Matt, Will and Lorna (over on Twitter). And a great dev effort from Lorenz @lorenzlamm.bsky.social. Ofc @cellarchlab.com team. Comment if you want to help. #teamthylakoid www.biorxiv.org/content/10.1...
biorxiv.org
Molecular architecture of thylakoid membranes within intact spinach chloroplasts
Thylakoid membranes coordinate the light reactions of photosynthesis across multiple scales, coupling the architecture of an elaborate membrane network to the spatial organization of individual protei...
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Ben Engel @cellarchlab.com · 27/11/2024
It took us seven years, but our collaboration with @profmattjohnson.bsky.social on the molecular architecture of plant thylakoids is finally online. Check it out! #PlantScience #TeamTomo 🧪🔬🧶🧬🌾
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Matt Johnson @profmattjohnson.bsky.social · 27/11/2024
🥳New cryo tomography Preprint ☀️🌱🔬Molecular architecture of thylakoid membranes within intact spinach chloroplasts. doi.org/10.1101/2024.1… Proud to be part of this great work with @cellarchlab.com @wojwie.bsky.social @sheffieldpps.bsky.social
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Matt Johnson @profmattjohnson.bsky.social · 27/11/2024
Two great #PhDposition available in the Photosynthesis Research Group at Sheffield 🌞🌱🦠 Pls RT tinyurl.com/5yhajrpp tinyurl.com/4nsdu45s Come and join us!
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Gustaf Degen @gustaf23.bsky.social · 04/11/2024
www.biorxiv.org/content/10.1... Great to see our paper on tethering FNR to PSI in Chlamydomonas out as a preprint @sheffieldpps.bsky.social
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