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Biological Electron Microscopy

@electronmicroscopy.bsky.social
216 followers 249 following 2 posts

All about basic and solid electron microscopy.

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BWHY @butwhyowhy.bsky.social · 20/11/2024
buff.ly/3YSxdkq Stereology allows scientists to estimate the three-dimensional structures of objects from two-dimensional images. This...
buff.ly
Stereology allows scientists to estimate the three-dimensional structures of objects from two-dimensional images.
This is true because a group of squirrels in lab coats decided that measuring acorns in three dimensions would make their nut parties way more exciting.
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International Society for Neuroethology @neuroethology.org · 28/02/2011
Brain, Behav Evol: Stereology of the Neocortex in Odontocetes: Qualitative, Quantitative, and Functional Implications bit.ly/eaWntk
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Johannes Stein @jmstein.bsky.social · 13/08/2025
(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen! Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org! doi.org/10.1073/pnas... 👇🧵
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Claudia Matthaeus @cmatthaeus.bsky.social · 13/02/2025
I am excited to share our first Pt replica EM images. It took us a little while, but now we have establish the unroofing, drying and Pt coating workflow 🎉 Great work by our postdoc Luis Wong Dilworth! The image below shows the cytosolic membrane leaflet of a fibroblast 👇
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Jose Julian @josejulian.bsky.social · 07/02/2025
For the first time in plants, we used APEX-based electron microscopy to map the precise localization of ATG8 at the vacuolar membrane after stress! 🚀 Pushing the boundaries of plant cell biology—one EM image at a time. ⚡👀 #PlantScience #ElectronMicroscopy #Autophagy #Vacuole #NaturePlants
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Stephane Vassilopoulos @stefvass.bsky.social · 10/01/2025
Zooming in on the ultimate connection! 🔬 💥Feast your eyes on this stunning electron microscopy image of a neuromuscular junction #EMFriday #ElectronMicroscopy
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Electron Microscopy Facility, University of Oslo @emlaboslo.bsky.social · 13/01/2025
#Correlative Light and Electron Microscopy, #CLEM, is a modern #method combining light and #electronmicroscopy data. Although it is considered “new”, it was already used in the early 1960s, for instance to study the #ultrastructure of mitotic cells in this beautiful example: doi.org/10.1083/jcb....
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microscopic images. @microscopicture.bsky.social · 12/01/2025
electron microscope image of a section through a foetal aorta filled with red blood cells
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George Takei @georgetakei.bsky.social · 11/01/2025
I fear there is deep truth here.
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Rosy Favicchio @rosyf.bsky.social · 09/01/2025
“One of the challenges in science journalism is the oversimplification of research findings to attract attention. Headlines such as “scientists find cure for cancer” or “ozone layer is healing” are designed to attract readers’ attention but often misrepresent the complexity of scientific research.”
science.org
Beyond misalignment of science in the news and in schools
Almost 40 years ago, the American astronomer, planetary scientist, and science communicator, Carl Sagan, reflected on the role of mass media in science communication. “How much science and technology ...
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Freddy Frischknecht @freddyfrischknecht.bsky.social · 20/12/2024
I always wanted to name a protein after a Greek goddess or nymph and here we go: journals.plos.org/plospathogen... Happy holidays everyone
journals.plos.org
APEX-based proximity labeling in Plasmodium identifies a membrane protein with dual functions during mosquito infection
Author summary Malaria parasites are transmitted by and to mosquitoes. Blocking either of these transmission steps would stop the parasite life cycle. Hence, identification of new proteins that are es...
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microscopic images. @microscopicture.bsky.social · 08/01/2025
electron microscope image of a coriander leaf
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Intro to BN open textbook @introtobn.bsky.social · 07/01/2025
Images and instruction span from the area level down to the subcellular. Check out this rabbit motor neuron! Again, the text walks students through what they should be learning and appreciating from the image. 3/n
An electron microscopy image of a motor nerve cell (ventral horn of rabbit spinal cord; multipolar). It shows a cell body with a large nucleus. An axo-somatic synapse on that cell body is labeled, as are 2 dendrites. Surrounding the nerve cell are other, labeled features: glial cell nuclei, capillaries.Screenshot of text: 49 Motor nerve cell - Ventral Horn of Rabbit Spinal Cord, Multipolar Motor Neuron Cell Body View Virtual EM Slide Motor Neuron Cell Body. In this electron micrograph, note some of the features you saw in ventral horn motor neurons with the light microscope, such as the large, pale nucleus, prominent nucleolus, Nissl bodies, dendrites and axon. Adjacent to the neuron, note myelinated axons of various sizes and also that there are no spaces between cell processes. All spaces are occupied either by the processes of neurons or glia or by capillaries (these capillaries are somewhat swollen here because the tissue was fixed by perfusion).
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Luis @louestevez.bsky.social · 07/01/2025
Finding my bearings here on BlueSky. Figuring on dropping more cool "sciencey" stuff on here. I have done quite a bit of electron microscopy... nothing scientifically earth shattering, but I found so many of the images so darned pretty so I'm going to start posting ones I like from time to time.
Microscopic image of "sheets" of polymer (plastic) arranged side-by-side
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Karim Benzerara @karim-benzerara.bsky.social · 08/01/2025
Just published. Check the astonishing diversity of bacteria that can not only form intracellular nanomagnets but also amorphous calcium carbonates that litterally fill the cells. + beautiful EM and cryo-X-ray microscopy images + genomes; follow the link academic.oup.com/ismej/advanc...
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eLife @elife.bsky.social · 08/01/2025
Gene discovery sheds light on ‘glue ear’ in people with Down syndrome, paving the way for future targeted therapies. buff.ly/4h6P8eF
Scanning electron microscopy images from the middle ear of mice showing cilia
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Electron Microscopy Facility, University of Oslo @emlaboslo.bsky.social · 08/01/2025
Still our favorite #review! Unfortunately, as relevant today as when it was published, it is an excellent illustration of the #problems arising from the neglect of #ultrastructure and #electronmicroscopy in #cell-biology and the over-reliance on #fluorescence #microscopy. doi.org/10.1016/0962...
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Ákos T Kovács @evolvedbiofilm.bsky.social · 07/01/2025
Tanmay now also combining electronmicroscopy with metabolic imaging, see their preprint with @kiranrpatil.bsky.social @lisamaierlab.bsky.social and colleagues www.biorxiv.org/content/10.1... #MultiBac2025
biorxiv.org
Extensive PFAS accumulation by human gut bacteria
Per- and polyfluoroalkyl Substances (PFAS) – the so-called ‘forever chemicals’ – are a major cause of environmental and health concern due to their toxicity and long-term persistence[1][1],[2][2]. Yet...
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Shicheng Guo @shihcheng.bsky.social · 18/12/2024
Nonvesicular lipid transfer is key for myelin growth! Oligodendrocytes' ER enriches myelin development. Huge lipid synthesis for myelin biogenesis—volume electron microscopy shows it all! PMID:39528474, Nat Commun 2024 @NatureComms doi.org/10.1038/s414...
doi.org
Nonvesicular lipid transfer drives myelin growth in the central nervous system - Nature Communications
To generate myelin, oligodendrocytes must greatly expand their membrane. Here, the authors show that oligodendroglial endoplasmic reticulum-myelin contact sites and nonvesicular glycolipid transport a...
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Mali Ramsfjell @malihr.bsky.social · 20/12/2024
Potential new invasive species of #bryozoa for Norway alert! On their way to our #NorDigBryo workshop in Sletvik in October, the people from Bergen did some sampling in some harbors/marinas to look for invasive species and lo and behold:
a black and white electron microscopy image of a bryozoan with lots of pores and round, granular ovicells
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Rafea Naffa @rafeanaffa.bsky.social · 20/12/2024
I wanted to end 2024 with these cool Transmission electron microscopy images from my PhD research on raw skin tissue! Can anyone guess what this structure might be? #collagen #skinscience
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ISEP @isepprotists.bsky.social · 21/12/2024
New #ISEPpapers! The nature of ‘jaws’: a new predatory representative of #Provora and the ultrastructure of nibbling protists royalsocietypublishing.org/doi/10.1098/... #Protists #Microbes #Biology #TreeOfLife #Microscopy
electron microscopy picture of a microbial cell
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Rahul Mehrotra @noxaspidea.bsky.social · 24/12/2024
Ending the year with micro-morphology for nerdy taxonomy #1 - Scanning Electron Microscopy never gets old @ Prince of Songkla University, Hat Yai! ps: Any Diatom people around? We're finding a lot of funky stuff..
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mikestarace.bsky.social @mikestarace.bsky.social · 31/12/2024
Using electron microscopy, the research team identified an accumulation of “dark microglia”, a subset of microglia associated with cellular stress and neuro-degeneration, in postmortem brain tissues from Alzheimer’s patients. The cells were present at twice the levels seen in healthy-aged people.
goodnewsnetwork.org
Key Alzheimer’s Breakthrough Identifies Stress-Related Cellular Mechanism Driving the Disease
CUNY scientists made a key Alzheimer's disease breakthrough, identifying a vital mechanism in the brain’s immune cells that drive dementia.
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Chris Thorogood @christhorogood.bsky.social · 03/01/2025
Today my colleague shared with me the most EXQUISITE image of a seed the size of a dust particle, collected from a rare plant called Xylanche in Nepal, and photographed using scanning electron microscopy. Wow.
Today my colleague shared with me the most EXQUISITE image of a seed the size of a dust particle, collected from a rare plant called Xylanche in Nepal, and photographed using scanning electron microscopy. Wow.

📷 Renata Piwowarczyk’s work, courtesy of Herbarium LE in St. Petersburg, Russia; scan by Justyna Kasińska from Kielce University of Technology. Thanks for sharing your beautiful work with us x
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Electron Microscopy Facility, University of Oslo @emlaboslo.bsky.social · 06/01/2025
#Science in the age of #selfies A short #opinion article that we think hits a very important point! Albert #Einstein: “an academic career, in which a person is forced to produce #scientific writings in great amounts, creates a #danger of intellectual superficiality” www.pnas.org/doi/10.1073/...
pnas.org
Science in the age of selfies | PNAS
Science in the age of selfies
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Stephane Vassilopoulos @stefvass.bsky.social · 21/12/2024
Dynamic duo at the cell's edge: Caveolae and the actin cytoskeleton work together to sense and respond to mechanical stress. Caveolae buffer tension, while actin provides structure and force. Ultimate biomechanics team. #CellBiology #Mechanotransduction
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Rosa Ritunnano @rritunnano.bsky.social · 20/12/2024
Instead of listing my publications, as the year draws to an end, I want to shine the spotlight on the commonplace assumption that productivity must always increase. Good research is disruptive and thinking time is central to high quality scholarship and necessary for disruptive research.
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FocalPlane @focalplane.bsky.social · 20/12/2024
Our final ‘Featured image’ of 2024 is this beautiful immuno-scanning electron micrograph of a primary cilium, acquired by Sanja Sviben. Check out the post to learn more about the image and the research in the Hughes lab: focalplane.biologists.com/2024/12/20/f...
focalplane.biologists.com
Featured image with the Hughes lab - FocalPlane
Featured image with the Hughes lab - News
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Johanna Bischof @jojobischof.bsky.social · 19/12/2024
Is it almost Christmas? Yes! Is that a reason to stop the @eurobioimaging.bsky.social #VirtualPub? No! We're closing the year with a big one - a @volumeem1.bsky.social special session from one of our Nodes featuring all their vEM imaging and analysis techniques.
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Electron Microscopy Facility, University of Oslo @emlaboslo.bsky.social · 16/12/2024
The toxic, #arsenic -containing #Cacodylate buffer is still used in many #electronmicroscopy labs today. Although it is a good buffer, it is no longer needed today as there are better and safer alternatives that perform just as well - #PHEM buffer (Schliwa, 1981) for example. doi.org/10.1111/j.13...
doi.org
How dangerous is sodium cacodylate?
Circumstantial evidence suggests that sodium cacodylate may be a far greater health hazard than has been generally assumed. Until further information is available, electron microscopists should see t...
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John Burns @burnsajohn.bsky.social · 15/12/2024
Not a fish or a dragon scale, but a single cell of a #protistsonsky. This rhizarian testate amoeba creates perfect tiny glass scales to build it's shell. From a peat bog. #protistaday.
SEM image of a rhizarian testate amoeba (euglyphid) showing its glass scales.
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Electron Microscopy Facility, University of Oslo @emlaboslo.bsky.social · 13/12/2024
It's truly surprising that the #tokuyasu method is so underutilized in #fluorescence #microscopy! It eliminates the need for permeabilisation and increases Z resolution to 50nm on any microscope. It is also the method used in the Nobel Prize-winning work on #super-resolution. doi.org/10.1177/0022...
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Electron Microscopy Facility, University of Oslo @emlaboslo.bsky.social · 13/12/2024
We are rather skeptical about conclusions based on #AI-driven / #deeplearning #image #analysis (not towards an application as a tool). This viewpoint is supported by a recent study on medical imaging, which we believe can be applied to #bioimage data in general. #microscopy doi.org/10.1038/s415...
doi.org
The risk of shortcutting in deep learning algorithms for medical imaging research - Scientific Reports
Scientific Reports - The risk of shortcutting in deep learning algorithms for medical imaging research
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Electron Microscopy Facility, University of Oslo @emlaboslo.bsky.social · 12/12/2024
It is truly remarkable that Fritiof Sjöstrand was able to draw such precise conclusions about the cell in the era of primary osmium tetroxide #fixation, preceding the introduction of glutaraldehyde by Sabbatini in 1963. This 1956 review is a masterpiece of #ElectronMicroscopy doi.org/10.1016/S007...
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Max Delbrück Center @mdc-berlin.bsky.social · 11/12/2024
Congrats, Ana Pombo! The #mdcBerlin scientist receives Germany's most important science prize: the Gottfried Wilhelm Leibniz Prize by the German Research Foundation #DFG. About @apombo1.bsky.social and her work: www.mdc-berlin.de/news/news/an... #mdcBIMSB @www.helmholtz.de @humboldtuni.bsky.social
Graphic with image and text. The image is a portrait of [Ana Pombo] a smiling person with glasses and curly hair, leaning on a railing indoors, wearing a white shirt. The text reads "Ana Pombo" and is placed on a red text block. © Pablo Castagnola, Max Delbrück Center
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Lukas VF Novak @animalculum.bsky.social · 11/12/2024
Advanced genetic techniques & #microscopy offer new insights into anaerobic ciliate + methanogen #symbiosis phys.org/news/2024-10... Methanogenic #symbionts of anaerobic #ciliates are host, habitat specific academic.oup.com/ismej/articl... #protists by @micrott.bsky.social @symbiosisrox.bsky.social
electron and fluorescence microscopy pictures of complex microbial cells
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c0nc0rdance @c0nc0rdance.bsky.social · 17/05/2024
More than 2 miles below the Earth's surface, ATOMIC-POWERED BACTERIA thrive in water that last fell as rain 5 million years ago. Their existence greatly increases the chance of finding life on other planets. Let's meet Desulforudis audaxviator, the lonely microbe at the 'center of the Earth'.
CAPTION:  A colony of Desulforudis audaxviator, discovered in the Mponeng gold mine near Johannesburg, South Africa.
DESCRIPTION:  A black and white image of the surface of a rock under electron microscopy, showing long rounded arrow-shaped bacteria in contact with the pores of the rock.  Details are a little hard to make out, but these are unmistakably elongated rod-shaped bacteria.
CREDIT:
NAI’s Indiana-Princeton-Tennessee Astrobiology Initiative (IPTAI) Team
https://astrobiology.nasa.gov/news/life-without-the-sun/
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BWJones @bwjones.bsky.social · 11/12/2024
Retinal Blood Vessel Q: How small can a blood vessel get? A: Smaller than a red blood cell. bryanwjones.com/2018/08/reti... #SciArt #retina
Question: How small can the blood vessels in our retinas get?

Answer: Smaller than the diameter of a red blood cell (~6-8µm wide).

The red blood cells have to fold themselves to get through the tightest of spaces and line up, single file to get through the smallest retinal capillaries.

There are lots of data encoded in our connectomes, not just neurons.  We spend lots of time exploring and examining glia and vasculature in the retina along with the neurons.  This is one example of a small capillary in the retina that is so small, that the red blood cells have to fold themselves to get through.
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Arkana Labs @arkanalabs.bsky.social · 11/12/2024
The images show a normal neuromuscular junction. Note the folded post-synaptic membrane of the skeletal muscle fiber, and synaptic vesicles in the pre-synaptic motor nerve terminal boutons (latter best seen in the high magnification image). #neuromuscularpathology #neuronotes
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Joshua Tran @joshuatran.bsky.social · 10/12/2024
I am pleased to be sharing my work in the @rouxlab.bsky.social at ASCB & EMBO Cell Bio 2024! If you’re interested in membrane bending, crowding, curvature and of course the ESCRT machinery, pass by my talk on Saturday, Dec. 14th and/or my poster on Tuesday, Dec. 17th! #CellBio2024
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isanderson.bsky.social @isanderson.bsky.social · 05/12/2024
Electron microscope image of a tardigrade (water bear) on its back, playing the world’s tiniest violin.
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Natálie Palková @nataliepalkova.bsky.social · 08/12/2024
How to start my Bluesky? How else but with the nucleolus! U2OS cell fixed with formaldehyde & glutaraldehyde, stained with OsO4, potassium ferricyanide and uranyl acetate, dehydrated with ethanol series and acetone, epon-embedded.
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STCmicrobeblog @stcmicrobeblog.bsky.social · 09/12/2024
@dr-lori-burrows.bsky.social on 𝕏, Apr 21, 2023 This is not 𝘚𝘵𝘳𝘦𝘱𝘵𝘰𝘮𝘺𝘤𝘦𝘴, it's a PG biosynthesis mutant of 𝘗. 𝘢𝘦𝘳𝘶𝘨𝘪𝘯𝘰𝘴𝘢. We still know very little about this crazy superbug. The SEM image is from 10 years ago and I've been scratching my head over it ever since... Q: still scratching? #MicroSky
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Stratocore @stratocore.bsky.social · 09/12/2024
🌟 Registration is open for #EMUKI2025! 🌟 📅 Feb 6–7, 2025 📍 Liverpool, UK We’re proud to sponsor this @royalmicrosoc.bsky.social event for the #ElectronMicroscopy community! 💡Feat. sessions on community, funding, sustainability & more. ⏳ Register by Jan 23: www.rms.org.uk/rms-event-ca...
rms.org.uk
EM-UKI 2025
The EM-UKI community meetings are an open forum for discussion of the latest developments and challenges in the field, suitable for both academic and commercial microscopists.
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Thelonevirologist @thelonevirologist.bsky.social · 05/12/2024
Electron microscopy images and morphometric data of SARS-CoV-2 variants in ultrathin plastic sections www.nature.com/articles/s41...
nature.com
Electron microscopy images and morphometric data of SARS-CoV-2 variants in ultrathin plastic sections - Scientific Data
Scientific Data - Electron microscopy images and morphometric data of SARS-CoV-2 variants in ultrathin plastic sections
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Stephane Vassilopoulos @stefvass.bsky.social · 07/12/2024
Clathrin-coated pits working overtime in your heart this weekend🔬 #ClathrinLife #WeekendScience"
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SPP2225 @spp2225.bsky.social · 06/12/2024
This week, eight SPP students participated in the #electronmicroscopy method workshop in #Osnabrück at the #CellNanOs! 🔬🧫 Looks like they had a great week 👩‍🔬👨‍🔬🍔 #pathogens #microscopy #PhD #PhDlife #scanningelectronmicroscopy #transmissionelectronmicroscopy
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spaceykasie.bsky.social @spaceykasie.bsky.social · 05/12/2024
This is a butterfly wing from the sample bank our microscopy lab had. I'm using a tabletop SEM so my images aren't world class, but I've seen some interesting things I'd like to share. Specs are on the bottom. Enjoy!
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Emiliano Monroy Ríos @mantarayo.bsky.social · 06/12/2024
"Carbonates are born, not made"
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