Schneider, Dussiau, and colleagues show that splenomegaly in myelofibrosis reflects active remodeling of the splenic stromal niche, not only extramedullary hematopoiesis. Reticular cell-driven complement activation disrupts lymphoid architecture and promotes fibrosis, identifying C3 as a targetable stromal axis.

An expanded atlas of human cells maps the expression of nearly all human transcription factors across 701 cell-type/tissue combinations and uncovers the heterogeneous nature of senescence across tissu...

This study uses single-molecule footprinting to quantify chromatin accessibility at enhancers and promoters in mouse embryonic stem cells and to dissect the contributions of transcription factor bindi...

Jia et al. develop SPACE-seq, a versatile technology that integrates CRISPR barcoding with spatial transcriptomics to jointly map cell ancestry, identity, and location. Compatible with diverse spatial platforms, SPACE-seq can be applied to a wide range of biological questions, revealing clonal architecture, migration, and fate-shaping interactions in development and cancer.

Over the past decade, advances in single-cell genomics, lineage tracing, and computational phylogenetics have transformed our understanding of hematopoiesi

Jia et al. develop SPACE-seq, a versatile technology that integrates CRISPR barcoding with spatial transcriptomics to jointly map cell ancestry, identity, and location. Compatible with diverse spatial platforms, SPACE-seq can be applied to a wide range of biological questions, revealing clonal architecture, migration, and fate-shaping interactions in development and cancer.

Single-cell and bulk lineage tracing reveals that adult bone marrow endothelial cells generate functional hematopoietic progenitor and mature blood cells, expanding the cell sources of postnatal hematopoiesis.

« Je ne sais pas comment mon successeur va boucler le budget l’an prochain. » Devant les sénateurs, l’actuel PDG du CNRS est revenu sur les contraintes budgétaires imposées à l’institution, malgré une...

‘Click clotting’ technology seals serious wounds in rats in seconds.

About Guest Posts The openRxiv blog includes occasional guest posts on different aspects of science communication and open research. This allows us to provide space for new perspectives from groups pi...

In this Perspective article, a theoretical framework for how the AP-1 family of transcription factors mediates cellular adaptation in cancer drug resistance is proposed.

Steroid-resistant acute graft-versus-host disease is set at disease onset and leads to distinct cell-to-cell cross-talk and immune trajectories.

UPDATE 01/29/2019. Read this awesome paper Statistical tests, P values, confidence intervals, and power: a guide to misinterpretations. This was an old post I …

Simpleaf is a command line toolkit written in rust that exposes a unified, simplified interface for processing single-cell and single-nucleus sequencing data using the alevin-fry pipeline. In this tut...

Ignoring a very informative lab test

A comprehensive single-cell atlas of AML uncovers regulatory networks and age-dependent molecular differences to advance the understanding of disease mechanisms and enable subtype-specific therapeutic...

To improve utility of cells with low UMI counts, I wanted to optimize KL weighting in SCVI models.

This work presents SUM-seq, an ultra-high-throughput method for co-profiling chromatin accessibility and gene expression in single nuclei across multiplexed samples, advancing the study of gene regula...

The STED-EC atlas enables inter-organ and multi-time-point comparisons of gene expression in endothelial cells, revealing substantial transcriptomic variations across endothelial cells from different ...