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Reposted by Alexandre Dumoulin

Melanie D. White @melaniedwhite.bsky.social · 01/10/2026

I spoke with @nature.com about the Nikon Small World controversy. As AI becomes part of scientific imaging, we need greater transparency about what was measured, inferred or generated. It's not about excluding AI. It’s about preserving trust in scientific evidence. www.nature.com/articles/d41...

nature.com

This award-winning microscopy image used AI — igniting controversy in a prestigious competition

Researchers say that using artificial-intelligence tools to visualize scientific images can become problematic when models misrepresent the underlying data.

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Alexandre Dumoulin @axonsalex.bsky.social · 12/09/2026

Can small extracellular vesicles guide growing axons?🧪 Our new preprint provides evidence that small extracellular vesicle (sEV) secretion regulates axon guidance at a key ventral midline choice point during spinal cord development. www.biorxiv.org/content/10.6... #neuroscience #EVs #axon

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Alexandre Dumoulin @axonsalex.bsky.social · 06/02/2026

Thanks! At a so early stage of the development (E5), they don't, but later for sure. It is a chick DRG explant.

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Alexandre Dumoulin @axonsalex.bsky.social · 06/02/2026

This cell migrates along axons and forms repeated adhesive contacts with their growth cones, creating a highly dynamic, almost dance-like behavior. Such contact-dependent interactions are common during nervous system development and help coordinate how cells and axons organize in space.

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Alexandre Dumoulin @axonsalex.bsky.social · 06/02/2026

A Sticky Dance Between a Cell and a Neuron. #neuroscience #science #mysterious #HiddenWorld #cell #FluorescenceFriday ➡️A Schwann cell precursor interacting with sensory axons 🔬🧫🧪

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Alexandre Dumoulin @axonsalex.bsky.social · 23/01/2026

This Is What Drives Axon Growth. Axon growth is powered by the actin cytoskeleton at the growth cone. Here, filamentous actin dynamics drive fast, directed axon extension in real time. #Fluorescencefriday #science #neuroscience #microscopy 🧪

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Reposted by Alexandre Dumoulin

Jeremy Baskin @jeremybaskin.bsky.social · 31/12/2025

Proximity labeling users: check out this study by @zhixingchen2.bsky.social and Peng Zou, which establishes how much contact-dependence vs. diffusion is responsible for biotinylation — tl;dr TurboID has a much smaller effective radius! www.nature.com/articles/s41... 1/2

nature.com

Spatial barcoding reveals reaction radii and contact-dependent mechanism of proximity labeling - Nature Chemical Biology

A DNA nanoruler platform enables precise measurement of the labeling radii of TurboID and APEX2, uncovering their contact-dependent mechanisms. These findings redefine the enzymology of proximity labe...

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Alexandre Dumoulin @axonsalex.bsky.social · 28/11/2025

Happy #FluorescenceFriday! Temporal projection of a time-lapse recording decided to become abstract art. Pretty splashy, isn't it? PalmBow-labeled commissural axons in culture. #neuroscience #microscopy #livecellimaging 🧪

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Alexandre Dumoulin @axonsalex.bsky.social · 28/11/2025

Hello, Yes, this video has been published as a cropped video in this paper: www.pnas.org/doi/10.1073/... also feel free to use this uncropped video for your lecture! Cheers

pnas.org

A gentle palette of plasma membrane dyes | PNAS

Plasma membrane (PM) stains are important organelle markers for monitoring membrane morphology and dynamics. The state-of-the-art PM stains are bri...

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Alexandre Dumoulin @axonsalex.bsky.social · 05/11/2025

Zurich is ready! 🤘 looking forward to hosting you at our Fish and Chicks seminar!

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Reposted by Alexandre Dumoulin

Victor Tatarskiy @greenrat.bsky.social · 02/10/2025

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

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Alexandre Dumoulin @axonsalex.bsky.social · 29/09/2025

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

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Reposted by Alexandre Dumoulin

Zhixing Chen @zhixingchen2.bsky.social · 16/07/2025

Check it out at PNAS today. Thank you Alexandre for the collaboration!! PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by Jing Ling, Yitong Liu, and all the collaborators! Available @spirochrome.com

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Alexandre Dumoulin @axonsalex.bsky.social · 16/07/2025

Here’s an embryonic axonal growth cone labeled with PK Mem dye (red) and tubulin (blue), imaged every 2 seconds. 🤩

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Alexandre Dumoulin @axonsalex.bsky.social · 16/07/2025

Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social ! We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience tiny.cc/3qhp001

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Reposted by Alexandre Dumoulin

Bassem Hassan @bassemh.bsky.social · 07/05/2025

Hot off the press: The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience: rdcu.be/ek010

rdcu.be

Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster

Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...

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Alexandre Dumoulin @axonsalex.bsky.social · 02/05/2025

cool! here are chick commissural axons crossing the neural tube floor plate :): bsky.app/profile/axon...

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Reposted by Alexandre Dumoulin

Kevin Mitchell @wiringthebrain.bsky.social · 30/04/2025

These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.

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Alexandre Dumoulin @axonsalex.bsky.social · 30/04/2025

Hi Luc, thanks! it is indeed Spy555-FastAct. Cheers

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Alexandre Dumoulin @axonsalex.bsky.social · 30/04/2025

it is a filopodial (thin protusions) party in there! 🕺🧐 🧪 #liveimaging

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Alexandre Dumoulin @axonsalex.bsky.social · 30/04/2025

This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging. I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉 🧪

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Reposted by Alexandre Dumoulin

Nikita Vladimirov @nvladimus.bsky.social · 20/01/2025

Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!

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Alexandre Dumoulin @axonsalex.bsky.social · 10/01/2025

me too! They seem to be retraction fibers that are then fragmenting into these small vesicles. It has been described in some migrating cells/cancer cells before. We are now inestigating it in the context of axon growth and guidance!

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Alexandre Dumoulin @axonsalex.bsky.social · 10/01/2025

Yes, it is, I wrote it in the ALT text but somehow it I can't see it. SPY555-FastAct

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Alexandre Dumoulin @axonsalex.bsky.social · 10/01/2025

And sometimes accross the floor plate 🙂

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Reposted by Alexandre Dumoulin

Roberto Mayor Lab @mayorlab.bsky.social · 04/01/2025

Join us in beautiful Quintay to celebrate 25 years of the Practical Course on Developmental Biology! To register go to biodesarrollo.unab.cl/mini-symposi... Come to enjoy a unique blend of science, sea, and food – all for free!

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Reposted by Alexandre Dumoulin

Addgene @addgene.bsky.social · 31/12/2024

Did you know 2024 was our 20th anniversary? We're closing it out by sharing a brief history of Addgene, with commentary from our incredible community. Read it below! blog.addgene.org/add...

blog.addgene.org

Addgene: The First Twenty Years

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

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Reposted by Alexandre Dumoulin

Danielle Beckman @daniellebeckman.bsky.social · 27/12/2024

2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)

Big blue neurons surrounded by small yellow microglia everywhere!

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Reposted by Alexandre Dumoulin

Zhixing Chen @zhixingchen2.bsky.social · 19/12/2024

How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling. www.biorxiv.org/content/10.1... Special thanks to @aliceyting.bsky.social for discussions

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Reposted by Alexandre Dumoulin

Lorena Benedetti @lorenabenedetti.bsky.social · 20/12/2024

I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social.

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Alexandre Dumoulin @axonsalex.bsky.social · 20/12/2024

For this #FluorescenceFriday, let's watch an axonal growth cone (for a change) displaying its most beautiful filopodia (this time)! 🤩 They are so dynamic! 1 image taken every 500 ms for 5 min played at 15 fps. 🧪

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Reposted by Alexandre Dumoulin

Philip Hubbard @philiphubbard.bsky.social · 20/12/2024

I used VVD Viewer for this #FluorescenceFriday video: 141 volumes from the #HHMIJanelia FlyLight Split-GAL4 #Drosophila driver collection. The animation comes from a high-level description processed by neuVid. Such a rapid tour of the data has questionable scientific value, but it's fun to watch!

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Reposted by Alexandre Dumoulin

Andy Moore @aaandmoore.bsky.social · 17/12/2024

Vimentin-mSG FRAP

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Reposted by Alexandre Dumoulin

Angelo Iulianella @aineurolab.bsky.social · 13/12/2024

Lynn Landmesser was a giant in the field of developmental neurobiology. Sad to hear of her passing. #DevBio #neuroscience 🧪

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Alexandre Dumoulin @axonsalex.bsky.social · 13/12/2024

It is showing off for sure! 🕺 Might be a piece of debris. Maybe a filopodium that still wants to party hard...

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Alexandre Dumoulin @axonsalex.bsky.social · 13/12/2024

This axonal growth cone from a culture of spinal neurons decided to display its most beautiful lamellipodia for this #FluorescenceFriday! 🤩 #neuroscience #liveimaging 🧪 1 image taken every second for 10 min, played at 15 images/s

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Alexandre Dumoulin @axonsalex.bsky.social · 11/12/2024

this overqualifies! you were already in the list. I just added your lab. cheers

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Reposted by Alexandre Dumoulin

Cedric Boeckx @cedricboeckx.bsky.social · 09/12/2024

Excellent preprint from @goodfrognosis.bsky.social’s lab, showing convergence of #autism related proteins at the #cilium (sure to interest people working on cilia/centrosome on this platform; ping @cilialab.bsky.social) www.biorxiv.org/content/10.1...

biorxiv.org

Convergence of autism proteins at the cilium

Hundreds of high confidence autism genes have been identified, yet the relevant etiological mechanisms remain unclear. Gene ontology analyses have repeatedly identified enrichment of proteins with ann...

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Reposted by Alexandre Dumoulin

Thibaut Brunet @thibautbrunet.bsky.social · 07/12/2024

Come to be our colleague @pasteur.fr! center of Paris, great colleagues, outstanding facilities, permanent position, crème brûlée every Friday - what else could one want?

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