Tools that track specific molecules in neurons have enabled researchers to probe previously unexplored aspects of neurobiology — although important caveats remain.

SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membr...

Deep neural networks have led to significant advancements in microscopy image generation and analysis. In single-molecule localization based super-resolution microscopy, neural networks are capable of...

Recent advances in fluorescence nanoscopy have pushed resolution to the 1-10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments. Achieving this...

A super-resolution microscopy technique offers an unparalleled glimpse into how monoclonal antibodies bind to their targets on cancer cells to induce cell death.

Very honored and grateful to receive the NSF CAREER Award! Big thanks to the National Science Foundation (NSF) and to my fantastic research team and…

Polymer-supported or tethered lipid bilayers serve as versatile platforms for mimicking plasma membrane structure and dynamics, yet the impact of polymer supports on lipid bilayers remains largely un...

Over the last 30 years, fluorescence microscopy, renowned for its sensitivity and specificity, has undergone a revolution in resolving ever-smaller details. This advancement began with stimulated emis...

Super-resolution microscopy (SRM) has transformed biological imaging by circumventing the diffraction limit of light and enabling the visualization of cellular structures and processes at the molecula...

Visualizing the ultrastructure of mitotic spindles, the macromolecular machines that segregate chromosomes during mitosis, by fluorescence imaging remains challenging. Here we introduce an azido- and ...

Rhobo6 is a cell-impermeable small-molecule fluorophore that displays reversible fluorogenic binding to glycans, making it a general, wash-free, and non-perturbative label for the extracellular matrix...

Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high t...

Various super-resolution fluorescence microscopy methods achieve nanoscale resolution, vital for visualizing subcellular structures. Choosing suitable biological standards is challenging, demanding p...

Nature Photonics - The authors present a review of super-resolution optical fluctuation imaging (SOFI), including its core working principle, recent advances and remaining challenges.

Elucidating the interaction between membrane proteins and antibodies requires whole-cell imaging at high spatiotemporal resolution. Lattice light-sheet (LLS) microscopy offers fast volumetric imaging ...