iiinova — where your digital self keeps evolving

Richard Held @rgsheld.bsky.social · 02/01/2026

Set my deadlift PR for the year 🤫

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Richard Held @rgsheld.bsky.social · 07/12/2025

Holds up: www.mcsweeneys.net/articles/the...

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derek guy @dieworkwear.bsky.social · 06/12/2025

The Nobel Prize committee should announce the World Cup winner tomorrow

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Cathy Spangler @cathyspangler.bsky.social · 24/11/2025

Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers! Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...

pnas.org

PNAS

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

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Richard Held @rgsheld.bsky.social · 01/12/2025

I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell. authors.elsevier.com/a/1mA6h,LqAr...

authors.elsevier.com

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cryoEM papers @cryoempapers.bsky.social · 30/11/2025

ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/41315814/ #cryoEM

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Richard Held @rgsheld.bsky.social · 18/11/2025

Clearly a poorly resolved flexible domain here

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Richard Held @rgsheld.bsky.social · 03/11/2025

Gonna need somebody to explain daylight savings to my dogs

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Richard Held @rgsheld.bsky.social · 09/10/2025

I think about this tweet on a near-daily basis

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Richard Held @rgsheld.bsky.social · 08/10/2025

This is what made America great

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Sven Truckenbrodt @sventruckenbrodt.bsky.social · 15/09/2025

The cat is officially out of the bag! The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix! This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠

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Richard Held @rgsheld.bsky.social · 08/08/2025

Heart rate data or RC voltage step?

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Richard Held @rgsheld.bsky.social · 17/07/2025

Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?

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Jennifer Waters @jencwaters.bsky.social · 23/06/2025

"The money the government sends to Harvard is, in effect, not a subsidy to advance the university’s mission. It’s a payment for the role Harvard plays in advancing the research mission of the United States." www.nytimes.com/interactive/...

nytimes.com

Here Is All the Science at Risk in Trump’s Clash With Harvard (Gift Article)

More than 900 research grants worth $2.6 billion are in jeopardy. So is the 80-year-old model of American science.

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Richard Held @rgsheld.bsky.social · 21/06/2025

Boston is the best city to run in. This is a take I'm willing to fight over.

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Richard Held @rgsheld.bsky.social · 21/06/2025

Having come up through US academia the rug pull of the last few months has been pretty extreme. Wondering how much longer I (& everyone else) get to do science sucks but with some dead-man's logic it also makes me really appreciate the years that I got to spend asking questions for their own sake.

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Michael Grange @michaelgrange.bsky.social · 17/06/2025

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

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Derek Lowe @dereklowe.bsky.social · 09/06/2025

A call to stand up and show support for the Bethesda Declaration, the NIH, and for federally funded science in general.

science.org

The Bethesda Declaration

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Richard Held @rgsheld.bsky.social · 02/06/2025

A formidable opponent in the fight against productivity.

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jankosinski.bsky.social @jankosinski.bsky.social · 30/05/2025

New method toward modeling cells and viruses! HMFF enables integrative membrane modeling and simulation guided by electron microscopy data. Created by Valentin Maurer and Marc Siggel @embl.org ‪‪@cssbhamburg.bsky.social‬, in collaboration with @WPezeshkian lab (1/n) www.biorxiv.org/content/10.1...

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Michael Baym @baym.lol · 14/05/2025

Yesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab

A screenshot of the termination notice showing "Outstanding Investigator Grants"

A screenshot of the termination notice with "This award is terminated effective the date of this award, due to unsafe antisemitic actions that suggest the institution lacks concern for the safety and wellbeing of Jewish students." highlighted

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Richard Held @rgsheld.bsky.social · 13/05/2025

Ok microtubule people, what is going on here? Cross section projection images, two different areas and surrounded by intact microtubules:

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Ricardo D. Righetto @lifeonthewedge.bsky.social · 07/05/2025

My colleagues @phaips.vd.st and @florentwaltz.bsky.social have put together this wonderful website with step-by-step tutorials for in situ subtomogram averaging using different software packages 🤩 Check it out, especially if you are a newcomer to the field! #CryoET #TeamTomo #ChlamyDataset

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Ricardo D. Righetto @lifeonthewedge.bsky.social · 23/04/2025

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...

Figure 4. MemBrain v2 end-to-end workflow detects periodic phycobilisome organization. A: Raw tomogram slice of EMD-31244. B: Out-of-the-box MemBrain-seg segmentation (light blue). C: A single membrane instance can be visualized in Surforama and manually annotated with GT phycobilisome positions (magenta). D: MemBrain-pick localizes particles (trained with data from C) on all membranes in the tomogram. E: MemBrain-stats computes Ripley’s O statistic using the positions from D with a bin size of 5nm. The distance between peaks (35 nm) was measured to estimate chain unit spacings.

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Sven Klumpe @svenklumpe.bsky.social · 09/04/2025

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

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bioRxivpreprint @biorxivpreprint.bsky.social · 06/04/2025

Exploring shaped focused ion beams for lamella preparation www.biorxiv.org/content/10.1101/202…

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Richard Held @rgsheld.bsky.social · 28/03/2025

Could run this article for every flight he takes

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Richard Held @rgsheld.bsky.social · 28/03/2025

Maybe Jeffrey Goldberg is in all of our signal chats and we just have to step back from our lives for a bit to really notice him there.

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Ed Twomey @cryoet.bsky.social · 26/03/2025

Here's our newest study: we reveal the molecular principles of neuronal excitation by glutamate, and how physiological temperatures influence this process. www.nature.com/articles/s41... www.nature.com/articles/d41... Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com

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Kelton Minor @keltonminor.bsky.social · 18/03/2025

There are days in life that shake you. I’m shattered 💔 to share that I just found out that the US Government terminated my 2024 NIH Director’s Early Independence Award (~$2 million), threatening my long-promised assistant professor job at Columbia University & academic career... 1/🧵

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Richard Held @rgsheld.bsky.social · 28/02/2025

Soapbox: acknowledging that the establishment of pax americana is often a morally complicated legacy does not require you to say there is no "wrong side of history." Nuance =/= nihilism and whataboutism is designed to paralyze you into believing you can never act in a principled manner.

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Richard Held @rgsheld.bsky.social · 25/02/2025

As flying in the US gets increasingly dicey, we will have to fall back on our rail system in order to travel safely. *whispered sidebar* oh I see...

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Reichow Lab @reichowlab.bsky.social · 21/02/2025

#NIHFundedDiscovery How do cells avoid the "kiss of death"? Gap junctions share life-saving signals—but under stress, they shut down to prevent spreading cytotoxic damage. #CryoEM reveals the molecular switches behind this process! www.biorxiv.org/content/10.1... www.biorxiv.org/content/10.1...

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Richard Held @rgsheld.bsky.social · 21/02/2025

Somewhere between this Derek Lowe quote and the Kendrick halftime show, you will find my current vibe

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Jack Iwashyna @iwashyna.bsky.social · 20/02/2025

Over on LinkedIn, the head of the Executive Secretariat of the NIH -- a central part of NIH leadership 🧪🩺-- resigned with a lettter worth reading www.linkedin.com/posts/nathan...

February 18, 2024
TO:
Dr. Matthew Memoli, Acting Director, NIH
CC:
John Burklow, Chief of Staff, NIH
Julie Berko, Director, OHR, NIH
FROM:
Nathaniel James Brought, Director, ES, NIH
SUBJECT: Resignation
Dear Dr. Memoli,
On July 3, 2001, I stepped off a bus on Marine Corps Recruit Training Depot Perris Island. Scared out of my mind, I stood on a pair of freshly painted yellow footprints, raised my right hand, and recited the oath of enlistment:
I, Nathaniel James Brought, do solemnly swear that I will support and defend the Constitution of the United States against all enemies, foreign and domestic; that I will bear true faith and allegiance to the same; and that I will obey the orders of the President of the United States and the orders of the officers appointed over me, according to regulations and the Uniform Code of Military Justice. So help me God.
For the last 23 years, 7 months, and 15 days, I like to believe I have faithfully carried out the duties of each office to which I've been appointed in my military and civilian service to this nation. That Service has taken me from the Marine Corps to 3 different federal departments, spanned 3 continents, included service in one war zone, and has included:
• For the Marine Corps and the National Security Agency, I worked on intelligence operations at the highest classification levels using bleeding edge intelligence tools to ensure America's special operators put boots-to-asses on America's enemies overseas (including commendations crediting my work for the kill or capture of dozens of terrorists), ensuring America's policy makers were able to track the movement of dangerous dual

nuclear technology across international borders, and monitored the flow of terrorist financing across the international banking system.
• Utilized information from all-source intelligence to ensure the continued security of America's homeland from international and domestic threats.
• Worked with some of the finest lawyers in the world to ensure America's security operations were effective, while upholding the rights of all those who interacted with them.
• Ensuring that America's rural communities had access to programs like rural development loans, farm aid, and that America's children wouldn't be hungry as they sat in their classrooms and tried to learn.
• Most recently, and frankly most dear to my heart, working with each of you here at the National Institutes of Health to advance the future of science and medicine. Not for Americans. Not for any one group of people. But for ALL of humanity.
I am unbelievably proud to be able to say that there are Americans who are alive, and terrorists who are not, because of the work I've done to serve this nation. I am proud to say that my service to this country has allowed me to ensure that my children have never faced the struggles of poverty that I grew up with. That service didn't begin because of some great altruistic impulse or drive. I didn't grow up saying "I want to do the great work that needs to be done to weave the fabric of America and ensure her people are not only safe, but healthy." Frankly, that service began because I was poor, and I was inspired. I grew up as a free lunch kid who lived in project housing. It was my fellow Americans who made sure I wasn't hungry in class and that I had enough food to excel academically the way I did. It was Americans who had more than we did that made sure I had good schools to attend where I could learn things that expanded my mind.
As I approached the end of high school, I dreamed of going to college and figuring out how to make a living that would allow me to do more tha…

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Richard Held @rgsheld.bsky.social · 20/02/2025

Window shopping for Scientifica rigs like pressing my nose against a Ferrari dealership

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Richard Held @rgsheld.bsky.social · 18/02/2025

Going to patch for the first time since 2018 (🤯). How long do we think it takes to get a decent trace? Like riding a bike, I'm sure 😬

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Richard Held @rgsheld.bsky.social · 14/02/2025

My small selfish bit of rage – insignificant compared to the existential threats to US science – is that the work of almost half my life is going really well and taking off into new directions and I would love to be happy and optimistic about that.

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