iiinova — where your digital self keeps evolving

Richard Held @rgsheld.bsky.social · 02/01/2026

I just went to the gym...but it's Jan. 1 so PR for the year

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Richard Held @rgsheld.bsky.social · 02/01/2026

Set my deadlift PR for the year 🤫

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Richard Held @rgsheld.bsky.social · 07/12/2025

Holds up: www.mcsweeneys.net/articles/the...

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derek guy @dieworkwear.bsky.social · 06/12/2025

The Nobel Prize committee should announce the World Cup winner tomorrow

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Cathy Spangler @cathyspangler.bsky.social · 24/11/2025

Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers! Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...

pnas.org

PNAS

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

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Richard Held @rgsheld.bsky.social · 01/12/2025

I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell. authors.elsevier.com/a/1mA6h,LqAr...

authors.elsevier.com

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cryoEM papers @cryoempapers.bsky.social · 30/11/2025

ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/41315814/ #cryoEM

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Richard Held @rgsheld.bsky.social · 28/11/2025

Thanks! The center ones didnt get quite the color I wanted but I'm happy with it.

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Richard Held @rgsheld.bsky.social · 18/11/2025

Clearly a poorly resolved flexible domain here

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Richard Held @rgsheld.bsky.social · 07/11/2025

Important to remember how fast the fusion process actually is! Millisecond experimental temporal resolution is hard to achieve but synchronous fusion is happening within hundreds of microseconds of action potential onset. www.nature.com/articles/384...

nature.com

Timing of neurotransmission at fast synapses in the mammalian brain - Nature

Nature - Timing of neurotransmission at fast synapses in the mammalian brain

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Richard Held @rgsheld.bsky.social · 07/11/2025

I'm not entirely on board with the proposed temporal sequence but I think the size of the dataset convincingly demonstrates the presence of "small" and "large" membrane proximal vesicle populations. A more tempting model imo would be that these small vesicles preferentially fuse asynchronously.

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Richard Held @rgsheld.bsky.social · 03/11/2025

Gonna need somebody to explain daylight savings to my dogs

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Richard Held @rgsheld.bsky.social · 02/11/2025

www.theatlantic.com/ideas/archiv...

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Richard Held @rgsheld.bsky.social · 09/10/2025

I think about this tweet on a near-daily basis

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Richard Held @rgsheld.bsky.social · 08/10/2025

This is what made America great

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Sven Truckenbrodt @sventruckenbrodt.bsky.social · 15/09/2025

The cat is officially out of the bag! The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix! This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠

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Richard Held @rgsheld.bsky.social · 08/08/2025

Heart rate data or RC voltage step?

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Richard Held @rgsheld.bsky.social · 17/07/2025

Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?

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Jennifer Waters @jencwaters.bsky.social · 23/06/2025

"The money the government sends to Harvard is, in effect, not a subsidy to advance the university’s mission. It’s a payment for the role Harvard plays in advancing the research mission of the United States." www.nytimes.com/interactive/...

nytimes.com

Here Is All the Science at Risk in Trump’s Clash With Harvard (Gift Article)

More than 900 research grants worth $2.6 billion are in jeopardy. So is the 80-year-old model of American science.

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Richard Held @rgsheld.bsky.social · 21/06/2025

Boston is the best city to run in. This is a take I'm willing to fight over.

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Richard Held @rgsheld.bsky.social · 21/06/2025

Having come up through US academia the rug pull of the last few months has been pretty extreme. Wondering how much longer I (& everyone else) get to do science sucks but with some dead-man's logic it also makes me really appreciate the years that I got to spend asking questions for their own sake.

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Michael Grange @michaelgrange.bsky.social · 17/06/2025

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

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Derek Lowe @dereklowe.bsky.social · 09/06/2025

A call to stand up and show support for the Bethesda Declaration, the NIH, and for federally funded science in general.

science.org

The Bethesda Declaration

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Richard Held @rgsheld.bsky.social · 09/06/2025

I believe @cmholab.bsky.social mentioned in a talk that they did something to assess this for plasmodium.

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Richard Held @rgsheld.bsky.social · 02/06/2025

A formidable opponent in the fight against productivity.

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jankosinski.bsky.social @jankosinski.bsky.social · 30/05/2025

New method toward modeling cells and viruses! HMFF enables integrative membrane modeling and simulation guided by electron microscopy data. Created by Valentin Maurer and Marc Siggel @embl.org ‪‪@cssbhamburg.bsky.social‬, in collaboration with @WPezeshkian lab (1/n) www.biorxiv.org/content/10.1...

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Michael Baym @baym.lol · 14/05/2025

Yesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab

A screenshot of the termination notice showing "Outstanding Investigator Grants"

A screenshot of the termination notice with "This award is terminated effective the date of this award, due to unsafe antisemitic actions that suggest the institution lacks concern for the safety and wellbeing of Jewish students." highlighted

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Richard Held @rgsheld.bsky.social · 13/05/2025

Ok microtubule people, what is going on here? Cross section projection images, two different areas and surrounded by intact microtubules:

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Ricardo D. Righetto @lifeonthewedge.bsky.social · 07/05/2025

My colleagues @phaips.vd.st and @florentwaltz.bsky.social have put together this wonderful website with step-by-step tutorials for in situ subtomogram averaging using different software packages 🤩 Check it out, especially if you are a newcomer to the field! #CryoET #TeamTomo #ChlamyDataset

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Richard Held @rgsheld.bsky.social · 29/04/2025

Not an arctis fan personally. The only real pro for arctis over hydra is the ability to take the cassette straight to Krios without having to worry about lamellae orientation.

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Ricardo D. Righetto @lifeonthewedge.bsky.social · 23/04/2025

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...

Figure 4. MemBrain v2 end-to-end workflow detects periodic phycobilisome organization. A: Raw tomogram slice of EMD-31244. B: Out-of-the-box MemBrain-seg segmentation (light blue). C: A single membrane instance can be visualized in Surforama and manually annotated with GT phycobilisome positions (magenta). D: MemBrain-pick localizes particles (trained with data from C) on all membranes in the tomogram. E: MemBrain-stats computes Ripley’s O statistic using the positions from D with a bin size of 5nm. The distance between peaks (35 nm) was measured to estimate chain unit spacings.

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Sven Klumpe @svenklumpe.bsky.social · 09/04/2025

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

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bioRxivpreprint @biorxivpreprint.bsky.social · 06/04/2025

Exploring shaped focused ion beams for lamella preparation www.biorxiv.org/content/10.1101/202…

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Richard Held @rgsheld.bsky.social · 28/03/2025

Could run this article for every flight he takes

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Richard Held @rgsheld.bsky.social · 28/03/2025

Maybe Jeffrey Goldberg is in all of our signal chats and we just have to step back from our lives for a bit to really notice him there.

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Ed Twomey @cryoet.bsky.social · 26/03/2025

Here's our newest study: we reveal the molecular principles of neuronal excitation by glutamate, and how physiological temperatures influence this process. www.nature.com/articles/s41... www.nature.com/articles/d41... Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com

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