iiinova — where your digital self keeps evolving

Reposted by Allegretti Lab

Casper Berger @casperberger.bsky.social · 23/09/2026

We developed Adaptive Polishing for the consistent preparation of ~150 nm thin FIB-lamellae. This was done by using imaging feedback to adjust parameters for each lamella, or Adaptive Milling, rather than applying a fixed recipe. Preprint: doi.org/10.64898/202...

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MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 01/09/2026

Using advanced microscopy @piotrkolata.bsky.social @a-dsantos.bsky.social @tomdendooven.bsky.social @matteoall.bsky.social have identified the molecular anatomy & organisation of proteasomes in the sperm nucleus. Read more about the study here: mrclmb.ac.uk/news-events/... #LMBResearch 🧪

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Tanmay Bharat @tbharat-lab.bsky.social · 08/06/2026

Disrupting phage liquid crystalline droplets restores antibiotic susceptibility in Pseudomonas aeruginosa biofilms out in @plosbiology.org by @abultarafder.bsky.social and team. Exciting collaboration with @geiselbiofilm.bsky.social @pearce-maths.bsky.social and others

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Allegretti Lab @matteoall.bsky.social · 27/05/2026

By making the biological content of cryoET data computationally accessible, easymode helps you screen and explore large datasets and use cellular context to identify particles — enabling flexible approaches to subtomogram averaging.

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Allegretti Lab @matteoall.bsky.social · 27/05/2026

As a showcase we used easymode to solve the in situ structure of filamentous IMPDH – the rate-limiting enzyme in GTP synthesis – at 4.0 Å resolution, and to map the cellular environment of this striking enzyme assembly.

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Allegretti Lab @matteoall.bsky.social · 27/05/2026

We validated the networks by solving structures for 10 distinct targets (15 maps total). Some directly via segmentation, others by combining the outputs of multiple tools – constraining picks to mitochondria, the nucleus, or the minus end of microtubules, for example.

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Allegretti Lab @matteoall.bsky.social · 27/05/2026

Work by @mgflast.bsky.social, @alisterburt.bsky.social, @tom-hale.bsky.social & @matteoall.bsky.social. With generous support from the incredible @mrclmb.bsky.social environment and others! More at mgflast.github.io/easymode

mgflast.github.io

easymode

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Allegretti Lab @matteoall.bsky.social · 27/05/2026

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Allegretti Lab @matteoall.bsky.social · 27/05/2026

To train general networks, the first step was assembling a large, diverse training set. In the end it comprised 4,000+ tilt series from 25+ species and 58 unique data sources, covering all common detector types and everything from purified samples to lifted-out tissue.

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Allegretti Lab @matteoall.bsky.social · 27/05/2026

We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇

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Reposted by Allegretti Lab

Sjors Scheres @sjorsscheres.bsky.social · 16/10/2025

I just made an account and voted for this biology laboratory made of LEGO to "promote biological research and inspire more people into the world of biology". They need 10K votes. Reposting and/or voting below would be helpful. 🤗 beta.ideas.lego.com/product-idea...

beta.ideas.lego.com

Biological Laboratory | LEGO® Ideas

This is a two-floor realistic Biological Laboratory building. In this design, I try to tell stories about biologists, introduce biology concepts to general publ…

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Reposted by Allegretti Lab

Lori Passmore @lapassmore.bsky.social · 25/02/2026

We are excited to be recruiting a new tenure track group leader in the Structural Studies Division at MRC LMB! It is an amazing place to start your own lab. @mrclmb.bsky.social Please get in touch if you have any questions. www.nature.com/naturecareer...

nature.com

Research Group Leader Tenure Track - Structural Studies - LMB 2775 - Cambridge, Cambridgeshire (GB) job with MRC Laboratory of Molecular Biology (LMB) | 12854636

Research Group Leader Tenure Track Starting Salary £67,979 to £76,868 per annum MRC Laboratory of Molecular Biology, Cambridge, UK The MRC Laborato...

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Wilfling Lab @wilflinglab.bsky.social · 13/01/2026

We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉

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Allegretti Lab @matteoall.bsky.social · 19/12/2025

This work is the result of fantastic teamwork! #Integrative structural cell biology @mrclmb.bsky.social, @biorxivpreprint.bsky.social

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Allegretti Lab @matteoall.bsky.social · 19/12/2025

Our findings reveal a unique splice variant and a trapped substrate intermediate never observed before, offering new insights into proteolytic catalysis. We also show proteasomes cluster from the post-meiotic spermatid stage, with striking human-mouse differences in proteasome organisation!

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Allegretti Lab @matteoall.bsky.social · 19/12/2025

We discovered giant membrane-less islands of proteasomes in human sperm nuclei, containing 20S and 20S-PA200 populations! We solved their in situ structure at sub-nanometer resolution (up to 6.8 Å) and in vitro at near-atomic resolution (1.8 Å)!

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Allegretti Lab @matteoall.bsky.social · 19/12/2025

We are thrilled to share our new pre-print on the molecular anatomy of nuclear proteasome in human sperm cells! #In-cellcryo-ET + #SPA + #LM! @piotrkolata.bsky.social @a-dsantos.bsky.social, @tomdendooven.bsky.social!! doi.org/10.64898/202...

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Reposted by Allegretti Lab

Ben Barad @baradlab.com · 12/12/2025

Holy cow the Zhou lab at UCLA is cooking with Isonet 2 - www.biorxiv.org/content/10.6... Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet

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Reposted by Allegretti Lab

Kiarash Jamali @kjamali.bsky.social · 09/12/2025

I’m excited to announce that I will be starting my research group on computational enzyme design at the Generative Biology Institute, Oxford, UK (@eitoxford.bsky.social)! PhD applications are open, deadline is Jan 8th (www.chem.ox.ac.uk/genbio-dtp). Email kiarash.jamali@eit.org with any questions!

chem.ox.ac.uk

GenBio DTP

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Reposted by Allegretti Lab

James Briscoe @jamesbriscoe.bsky.social · 07/10/2025

Very sad news, John Gurdon has died. A developmental biologist's developmental biologist, Nobel prize winner His work is the foundation of much of today's dev & stem cell bio. An inspiration to many, including me. Always asking questions & wanting the answers www.magd.cam.ac.uk/news/profess...

magd.cam.ac.uk

Professor Sir John Gurdon FRS (1933-2025) | Magdalene College

Magdalene College is deeply saddened to announce the death of Professor Sir John Gurdon FRS, who served as Master of the College from 1995 to 2002.

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Reposted by Allegretti Lab

Martin Pacesa @martinpacesa.bsky.social · 27/08/2025

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...

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Allegretti Lab @matteoall.bsky.social · 27/08/2025

Many congratulations!! Really great work!

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Reposted by Allegretti Lab

MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 11/08/2025

We’re looking for a Research Support Officer to join @matteoall.bsky.social’s group, working with @piotrkolata.bsky.social to investigate nuclear-cytoskeletal coupling in mammalian cells using #cryoEM, #cryoET & other biochemical approaches. www.jobs.ac.uk/job/DOE317/r... Closes 31 AUG #ScienceJobs

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Allegretti Lab @matteoall.bsky.social · 05/08/2025

Also check out the work of @tzviyazbm.bsky.social on the same topic!

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Allegretti Lab @matteoall.bsky.social · 05/08/2025

It was a lot of fun and a tremendous synergy of intents dear @rezamehdipour.bsky.social! Thank you

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Reposted by Allegretti Lab

A. Reza Mehdipour @rezamehdipour.bsky.social · 05/08/2025

We discovered that the SUN5-Nesprin3 LINC complex forms a hexagonal lattice at the sperm HTCA, which radically reshapes the nuclear membrane locally! Our findings also provide the structural basis for understanding the severe ASS (Acephalic Sperm Syndrome) male infertility phenotype.

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Reposted by Allegretti Lab

A. Reza Mehdipour @rezamehdipour.bsky.social · 05/08/2025

This work is the result of a fantastic collaboration with@matteoall.bsky.social! @warred.bsky.social, @a-dsantos.bsky.social , @alisterburt.bsky.social , @tom-hale.bsky.social, @mrclmb.bsky.social,

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Reposted by Allegretti Lab

A. Reza Mehdipour @rezamehdipour.bsky.social · 05/08/2025

We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!! www.biorxiv.org/content/10.1...

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Allegretti Lab @matteoall.bsky.social · 01/08/2025

Dear all, we are looking for a research assistant in protein biochemistry / cell biology to support our work on nuclear reshaping in human cells. Here the link to the call, please spread: mrc.tal.net/vx/mobile-0/...

mrc.tal.net

Research Support Officer - Structural Studies - Dr Matteo Allegretti - LMB 2674 - Medical Research Council

Location: Cambridge. Vacancy: Research Support Officer - Structural Studies - Dr Matteo Allegretti - LMB 2674. Closing Date: 31/08/2025, 23:55

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Reposted by Allegretti Lab

Ricardo D. Righetto @lifeonthewedge.bsky.social · 27/07/2025

Using #cryoET to investigate plant cell-cell communication 🤩🌿

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Tanmay Bharat @tbharat-lab.bsky.social · 23/06/2025

Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDAphi Out in @pnas.org Congrats @janboehning.bsky.social @abultarafder.bsky.social Miles Graham @mathieucoureuil.bsky.social @mrclmb.bsky.social @hopitalnecker.bsky.social www.pnas.org/doi/10.1073/...

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Derivery Lab - MRC LMB @deriverylab.bsky.social · 23/06/2025

🚨 JOB ALERT 🚨 We're hiring a Research Assistant – no PhD needed! Join us to explore the molecular mechanisms of receptor sorting in endosomes. Experience in protein expression/purification, cloning & cell culture desired 🧫🔬 Apply now 👉 www.nature.com/naturecareer... #STEMCareers #ResearchJobs

A picture of 3 rows of people on steps at the MRC LMB. From left to right - top row: Akaash, Vic, Lara - Middle row: Kerrie, Manu, Paddy - bottom row: Meg and Julie

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MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 05/06/2025

Congratulations to @tbharat-lab.bsky.social, Group Leader in the LMB’s Structural Studies Division, who has received the Gold Medal from @embo.org for his research on prokaryotic surface molecules. Read more here: www2.mrc-lmb.cam.ac.uk/tanmay-bhara... #LMBNews

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Phil Holliger @philholliger.bsky.social · 29/05/2025

New paper from my group rdcu.be/eoaRr "Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme" in Nature Chemistry — great collaboration with attwaterlab.uk.

rdcu.be

Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme

Nature Chemistry - Models of abiotic RNA replication suffer from inherent product inhibition arising from the high stability of RNA duplexes. Now, it has been shown that RNA trinucleotide...

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Reposted by Allegretti Lab

Ben Engel @cellarchlab.com · 07/05/2025

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

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Gaia Pigino @gaiapigino.bsky.social · 19/02/2025

“Join the Pigino Lab! We’re hiring a PhD student to explore primary cilia biology using advanced imaging, spatial ‘omics, and AI. Apply now at www.cilia-ai.eu and be part of the Cilia-AI Doctoral Network!”

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Tanmay Bharat @tbharat-lab.bsky.social · 23/04/2025

Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.

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Suyang Zhang @suyangzhang.bsky.social · 24/03/2025

Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein. www.biorxiv.org/content/10.1... #cryoEM #transcription #splicing

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Sven Klumpe @svenklumpe.bsky.social · 05/03/2025

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

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Reposted by Allegretti Lab

GW Gant Luxton @gwgl.bsky.social · 21/02/2025

science.org/doi/10.1126/... Just published! We uncovered a molecular machine that connects the cell's two major structural networks revealing how cells accomplish precise internal reorganization - a process disrupted in diseases ranging from cancer to multiple sclerosis.

science.org

Active microtubule-actin cross-talk mediated by a nesprin-2G–kinesin complex

Nesprin-2 Giant links the nucleus to the cytoskeleton, enabling actin transport and nuclear movement through kinesin-1 activation.

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Allegretti Lab @matteoall.bsky.social · 10/02/2025

Huge congratulations and best of luck with the new adventure Sven!

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Reposted by Allegretti Lab

Sjors Scheres @sjorsscheres.bsky.social · 27/01/2025

We have an exciting opportunity for a new independent group leader with an interest in structural/molecular biology and machine learning to join our Division of Structural Studies at the @mrclmb.bsky.social! 🥳 Re-posts appreciated. www.nature.com/naturecareer...

nature.com

Research Group Leader Tenure Track - Structural Studies - LMB 2549 - Cambridgeshire job with MRC Laboratory of Molecular Biology | 12835196

Research Group Leader Tenure Track Starting Salary £65,940 to £74,448 per annum MRC Laboratory of Molecular Biology, Cambridge, UK

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Allegretti Lab @matteoall.bsky.social · 20/01/2025

The work also explains how this mechanism is selective for cancer cells and not healthy cells!! Enjoy the read! @a-dsantos.bsky.social, @piotrkolata.bsky.social, @mrclmb.bsky.social

trkolata.bsky.social

Bluesky

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