Single-cell proteomics can reveal cellular heterogeneity but remains challenging for small cells. Here, the authors establish a sensitive label-free workflow that quantifies up to 7,000 proteins per HeLa cell and up to 4,000 proteins in small human immune cells.

Members of the LRRC8 family form heteromeric ion channels that are activated upon cell swelling. Here authors describe the architecture of assemblies of LRRC8A and D subunits and their conformational changes during activation.

Ion channels of the LRRC8 family are modular proteins consisting of a transmembrane pore and cytoplasmic regulatory domains. Here the authors combine structural and functional data to define the role of the intracellular sub-domain (ISD) of the obligatory LRRC8A subunit for channel activation.

Proteasomes can be capped by regulatory particles other than the 19S complex, but how these form is unclear. Here, authors reveal cryo-EM structures of Blm10-proteasome assembly intermediates and provide evidence for parallel assembly pathways.

AMPA receptors mediate the majority of excitatory neurotransmission. Here, the authors report structural and functional regulation of GluA2 AMPA receptor by inhibitory auxiliary subunits GSG1L and TARP γ5 and by epilepsy-associated disease mutations.

Lipid transfer proteins organize intracellular lipid distribution. Here, the authors show that seemingly redundant phospholipid transfer proteins have distinct acyl chain preferences, and suggest that their tissue-specific expression levels and phosphorylation fine-tune membrane composition.

The PfPCRCR complex is essential for invasion of human erythrocytes by the malaria parasite, Plasmodium falciparum. Here, authors structurally and functionally characterise antibodies’ interactions with PfPCRCR, with implications for the design of PfRIPR-based anti-malarial vaccines.

The allosteric mechanisms by which lipid binding controls protein activity remain poorly understood. Here, the authors identify a lipid-triggered allosteric site in LC3 that remodels its receptor-binding interface upon membrane interaction. This site can be engineered to precisely tune LC3-LIR interactions, offering a new strategy to regulate selective autophagy.

Protein citrullination by PAD enzymes is vital for cell function but isozyme specificity is poorly understood. Here, the authors map thousands of in vitro modification sites across the active human PAD family to reveal the landscape and structural rules governing substrate recognition.

Here, the authors introduce deep receptor scanning methodology to enable characterization of 940 human GPCRs in parallel. This approach can be coupled to a variety of assays to systematically profile the activity and pharmacology of nearly all human GPCRs.

The endosomal Na+ /H+ exchanger HsNHE6 regulates organellar pH and is implicated in neurological disease. Here the authors determine its cryo-EM structure and show a kinetic preference for K+ over Na+ transport.

In this work, the authors develop and characterize KIT/PDGFRA inhibitors to define key molecular interactions underlying inhibitor selectivity and drug-resistance, reporting structural insights for targeting solvent-front mutations across oncogenic kinases.

SUMO ligases conjugate the SUMO modifier to target proteins, thus regulating their functions. Here, the authors reveal how DNA and ATP binding by the multiple subunits of the SUMO ligase complex Smc5/6 enable its efficient SUMOylation of genome maintenance proteins.

Advances in electron microscopy instrumentation and methods continue to drive progress in the physical and life sciences. The 4th edition of this conference will bring together researchers from physic...

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Nature - Ubiquitination of glycogen and metabolites in cells and tissues

The research group Ubiquitin Signaling Specificity (Dr. Sonja Lorenz) invites applications for the position as PhD student or Postdoc (f/m/d) – Understanding and manipulating ubiquitin ligase specifi...

The International Day for the Elimination of Violence against Women aims to create awareness of the fact that violence against women is a human rights violation that impedes progress in many areas, in...

Nature - Molecular basis of SIFI activity in the integrated stress response

O-GlcNAcylation can regulate protein properties and functions. Here, the authors systematically study its impacts on protein solubility and biocondensate formation. O-GlcNAc dramatically reduces prote...

Job Title: Associate or Senior Editor (Organic Chemistry), Nature Communications Organisation: Nature Portfolio Locations: Jersey City, London, New York, Philadelphia or Washington DC - hybrid working...

Proteasomal degradation is a fundamental process for all eukaryotic life. A protein destined for degradation is first tagged with a polyubiquitin chain, which is selected by the proteasome. Different ...

Chip-Tip is a label-free quantification-based single-cell proteomics workflow for deep single-cell proteomics, which identifies over 5,000 proteins and 40,000 peptides in single HeLa cells.

ATG8ylation of the tonoplast, triggered by cell wall damage, acts as a vital vacuolar quality control mechanism that safeguards vacuolar integrity and ensures cell survival under stress.

SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.

LC-MS-based proteomics often relies on data-dependent acquisition (DDA) for quality control. Here, the authors demonstrate that data-independent acquisition (DIA) outperforms DDA in detecting subtle c...

Visualization and picking of membrane proteins in cryoET tomogram is often challenging. Here, the authors developed a software MPicker based on membrane flattening to simplify the visualization, picki...

Shen and Yin developed a technology named FUSEP to profile the E2-specific ubiquitome that makes use of a fusion between E2 and ubiquitin carrying a R74G point mutation, which leaves an LGGG remnant f...