Ion channels of the LRRC8 family are modular proteins consisting of a transmembrane pore and cytoplasmic regulatory domains. Here the authors combine structural and functional data to define the role of the intracellular sub-domain (ISD) of the obligatory LRRC8A subunit for channel activation.

Members of the LRRC8 family form heteromeric ion channels that are activated upon cell swelling. Here authors describe the architecture of assemblies of LRRC8A and D subunits and their conformational changes during activation.

Individual proteins tend to adopt preferred orientations when subjected to vitrification for cryo-electron microscopy analysis. A laser flash melting procedure followed by rapid revitrification provid...

IST2 serves as a tether between the endoplasmic reticulum and the plasma membrane in yeast. Here, Arndt et al. interrogate its interaction with OSH6, revealing that the two proteins remain associated ...

The Tweety homologue TTYH2 is identified as the lipid transfer mediator for APOE-containing lipoproteins.