iiinova — where your digital self keeps evolving

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Daniel Mann @proteincommandr.bsky.social · 03/07/2026

Bottomless Krios 😱

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Sonja Welsch @sonjawelsch.bsky.social · 30/06/2026

Thought I had discovered a new #cryoEM “hole size gradient” feature. Turns out E.coli in ice creates optical illusions ☹️. Or it’s just me hallucinating.

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Harry M. Williams @harrywilliams.bsky.social · 02/07/2026

Dear #cryoem community, does anyone if its possible disable atlas categorization in EPU? And if so, how?

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Ben Barad @baradlab.com · 13/06/2026

I had such a fun time teaching at the U Michigan Cryo-ET Course this week! I taught quantitative ultrastructure analysis and we made cool figures like these! My course materials are at baradlab.com/michigan_tutorial for anyone to try. Workflow include mesh refinement and thickness measurement!

ChimeraX/ArtiaX visualization showing mitochondria-associated ribosomes connecting drives reduced IMM-OMM spacing in yeast.

Surforama depiction of mitochondrial outer membrane shows membrane-associated ribosomes.

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Sonja Welsch @sonjawelsch.bsky.social · 29/07/2025

A #FIBSEM #cryoET drama in 4 acts. Prelude: Aquilos shuttle rod does not reach cryo stage due to mechanical misalignment.

1 of 2 alignment pins on Aquilos load lock has mysteriously disappeared.

4 pairs of eyes, 4 hours and one treasure hunt later, the alignment pin resurfaces in the most obscure spot.

The stray alignment pin is superglued into place (why isn’t this the default anyway)

Load lock back in working order - sample unloading can proceed. Happy end.

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RCSB Protein Data Bank @rcsbpdb.bsky.social · 21/04/2025

Introducing Extended PDB IDs at RCSB PDB Search, view, and download all PDB structures using their extended PDB ID. wwPDB anticipates that all four-character PDB IDs will be exhausted by 2028, after which only 12-character PDB IDs will be issued. www.rcsb.org/news/fe...

rcsb.org

Introducing Extended PDB IDs at RCSB.org

Search, view, and download all PDB structures using their extended PDB ID. wwPDB anticipates that all four-character PDB IDs will be exhausted by 2028, after which only 12-character PDB IDs will be issued.

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Imaging Artifact @imagingartifact.bsky.social · 21/04/2025

Someone please stop the 3DEM auto-reply madness #cryoEM

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zrizvi.bsky.social @zrizvi.bsky.social · 21/04/2025

First post here- Hello Bluesky! Quick science query: These 2D classes are from negative stain of a FLAG pulldown (HEK cell eluate). Does this look like a real complex or a common contaminant? Would live to hear from anyone who’s worked on similar prep! #negativestainEM #pulldown #HEKcells

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Utz Ermel @uermel.bsky.social · 06/03/2025

Results from CZII's #cryoET object detection challenge on kaggle are in! Participation exceeded our expectations with 931 Teams from 76 countries competing, generating over 27,000 submissions. For an overview check out our post-competition page: cryoetdataportal.czscience.com/competition

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Kyle Harrington @kyleharrington.com · 06/03/2025

Check this out!!! The results from our CryoET particle picking competition are available, the full test data (public and private datasets) is available, and you can dive right into the data on the CZ CryoET Data Portal! Enjoy and please let us know what you think :)

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Yousuf A. Khan @yousufakhan.bsky.social · 04/03/2025

I never felt such joy as I have until I temporarily upgraded to COOT 1, realized how terrible it is, and downgraded back to my old friend Coot 0.9. #CryoEM #TeamTomo

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Lorna Malone @drlornamalone.bsky.social · 17/01/2025

📢 Registration is now open for the @diamondlightsource.bsky.social Biological Cryo-imaging User Meeting 2025! 📍Join us in Nottingham on April 23, in-person & online. Plus, don’t miss the CCP-EM Spring Symposium (April 24-25)🦠#CryoEM #BCIUM2025 🔗Register here: www.diamond.ac.uk/Home/Events/...

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Basil Greber @bjgreber.bsky.social · 12/12/2024

The microscope comparison datasets (Glacios vs. Krios, EF vs. no EF) from our high-resolution/high-throughput #cryoEM SBDD workflow project (the one with more than a dozen ligand-bound 2 Å structures of an 85 kDa complex) have been released on EMPIAR: www.ebi.ac.uk/empiar/EMPIA...

ebi.ac.uk

EMPIAR-11821 Cryo-EM of human CAK: Microscope comparisons

EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-ser...

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Dennis J. Michalak @dmichalak.bsky.social · 10/12/2024

Was thinking about RELION5's new coordinate system and came to a realization... Was the switch to a centered coordinate system done to make sure that the coordinates of a particle are applicable to reconstructed tomograms of any size? #teamtomo

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Imaging Artifact @imagingartifact.bsky.social · 11/12/2024

But what is the resolution cutoff that distinguishes near from close to?

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Kyle Harrington @kyleharrington.com · 08/12/2024

For the #ML and #CryoET folks out there: Are folks on here submitting to the CZII CryoET Object Identification competition: www.kaggle.com/competitions... ? The leaderboard is really interesting, and the forums are full of insights. How many of the latest picking models have been submitted?

kaggle.com

CZII - CryoET Object Identification

Find small biological structures in large 3D volumes

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Edward T Eng @edwardteng.bsky.social · 09/12/2024

We received our TFS Arctis this past weekend. This #cryoEM instrument is part of our initiative to expand the #cryoET biomedical research capabilities at the center. SEMC/NYSBC - Providing expertise and resources for understanding both molecular and cellular structures. Find out more at: nysbc.org

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Sjors Scheres @sjorsscheres.bsky.social · 07/12/2024

The underlying theory (in Fourier space) isn't too far away from that of crystallography. Hopefully the #relion tutorial and the #cryoEM video lectures at @mrclmb.bsky.social's YouTube channel will help too. 🤗

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Imaging Artifact @imagingartifact.bsky.social · 06/12/2024

Cryosparc isn't the only tool you should RELION 🥲 #cryoEM

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klbr @klbr.bsky.social · 05/12/2024

I'm going to run an intensive "boot camp" called "Practical Cryo-EM" where we learn how to freeze grids, align microscopes, collect images, and do 3D reconstruction and stuff. January 13-17

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Daniel Hurdiss @danielhurdiss.bsky.social · 30/11/2024

Greatly enjoying our ongoing #cryoET adventures. My new favourite way to procrastinate from administrative tasks is to play I spy with @bobcoelingh.bsky.social's recent data set! 👀 Here's an easy one! 👇

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Jess Heebner @jessheebner.bsky.social · 01/12/2024

Anyone have any publicly available data (or data you want to share with me) that you'd love to see segmented? I find myself in need of some fun data to segment in my free time... #cryoET or #volumeEM!

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Imaging Artifact @imagingartifact.bsky.social · 05/12/2024

What did you listen to most this year? #CryoEM Reposting this old meme on the new platform

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King Cada @kingcada.bsky.social · 04/12/2024

Particle looks like a happy puppers eager to see me. #cryoEM

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Bui Lab @McGill @builab.bsky.social · 22/09/2021

#TeamTomo What is the most user-friendly template matching software for cryo-ET with samples with rather low complexity? Can't do Warp now without Windows machine.

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Bui Lab @McGill @builab.bsky.social · 07/12/2021

My first contribution to #TeamTomo . Script to help visualize the subtomogram orientations from #Relion star file inside its tomogram using ChimeraX. Thanks to @AlisterBurt for eulerangles & starfile module. github.com/builab/subtomo…

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Utz Ermel @uermel.bsky.social · 24/11/2024

FYI: Thanks to @cellarchlab.com's awesome open science practices, this data is available on the CZ cryoET data portal (dataset 10008) and @ebi.embl.org's EMPIAR (11058)! You can check out the tomograms in your browser here: cryoetdataportal.czscience.com/datasets/10008

cryoetdataportal.czscience.com

Cryo-electron tomograms of T. kivui of FIB-SEM lamella.

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Basil Greber @bjgreber.bsky.social · 25/11/2024

Some #cryoEM people: The cup can only ever be 2/3 full!

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Bridget Carragher @bcarra.bsky.social · 24/11/2024

CZII’s cryoET particle picking machine learning challenge is up and running. More than 3400 signups on Kaggle, great discussions going on, and leaderboard scores better than we were able to do! Start off at: cryoetdataportal.czscience.com/competition

cryoetdataportal.czscience.com

Started

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Imaging Artifact @imagingartifact.bsky.social · 16/11/2024

There has to be a better way to do this. Anyone found a creative solution?

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Dominik Hrebik @dhrebik.bsky.social · 14/11/2024

I will slowly start (re)posting the #cryoEM #Science #Memes from my Tw/X profile. Let's start with this one

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Veronica Falconieri Hays, MA CMI @falconierivisuals.bsky.social · 15/11/2024

Let's get caught up, shall we? Over the next few weeks I'm going to share #sciart highlights from my 10 years as a professional medical illustrator. First up, my 2014 image of beta-galactosidase, created in collaboration with Doreen Matthies (NIH), illustrating #cryoem resolution breakthroughs

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King Cada @kingcada.bsky.social · 13/11/2024

Vesicle getting engulfed by forming autophagosome inside axons. #cryoET #teamtomo

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Richard Held @rgsheld.bsky.social · 13/11/2024

Reintro: I'm a neuroscientist interested in how cells use molecular topography to control signal transduction. I use #CryoET and mouse genetic tools to map the topography of synaptic proteins and determine in-situ structures of scaffolding interactions that control said topography.

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Ben Barad @baradlab.com · 15/11/2024

Seems like its time for a re-introduction. I am a junior faculty at OHSU and a #teamtomo #cryoET enthusiast with a particular interest in membrane reorganization within mammalian cells. I develop the Surface Morphometrics toolkit (and some other bits and bobs).

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Yousuf A. Khan @yousufakhan.bsky.social · 15/11/2024

A proper intro: I'm at Stanford currently and I study (3) things 🧪 1) #cryoEM and #cryoET #teamtomo of molecular machines: We put out a preprint on how the AAA+ machine Sec18 bypasses topology of all SNARE substrates by using 'side-release' and 'side-loading' (www.biorxiv.org/content/10.1...) 1/3

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Ieva Drulyte @ievadr.bsky.social · 14/11/2024

🌌 Seeing cryo-EM picking up on Bluesky, it's time for a reintroduction! 👋 I'm Ieva, a structural biologist (former crystallographer turned cryo-EM aficionado since 2014). I work at Thermo Fisher Scientific and am passionate about advancing #cryoEM & #cryoET applications in #DrugDiscovery 🔬💊

A selfie featuring cryo-EM autogrid box earrings 📸

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Rémi Fronzes @fronzeslab.bsky.social · 15/11/2024

To the #CryoET folks: Is there any advantage to collecting tilt series in EER (vs. good old Tiff format.) for in situ CryoET? I saw a meeting report from July 2024 on the web related to this question, but could not find a publication (yet).

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Rémi Fronzes @fronzeslab.bsky.social · 08/11/2024

Here is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksC

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Ben Barad @baradlab.com · 16/11/2024

Starter packs are great for a maximally twitter-like experience, but I've been really into feeds, which let me sort by content rather than by specific people. Here's one for #teamtomo bsky.app/profile/did:...

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Paolo Swuec @pscryoem.bsky.social · 04/07/2024

𝐄𝐥𝐞𝐜𝐭𝐫𝐨𝐧 𝐌𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲 𝐒𝐩𝐞𝐜𝐢𝐚𝐥𝐢𝐬𝐭 post: deadline extended! Please, share it with whoever is seeking professional growth. "Junior" profiles strongly encouraged, you'll be put on the right training track by our Specialists. careers.humantechnopole.it/o/electron-m...

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drjay.bsky.social @drjay.bsky.social · 05/02/2024

We got alpha helices ❄️🔬

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Ben Barad @baradlab.com · 08/02/2024

If you don't have or want to use a personal or lab website and do cryo-ET I'll set you up with a @NAME.tomo.science handle.

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Stephen Graham's Lab @atomicvirology.bsky.social · 10/02/2024

Very excited that our pathogen cryo microscopy facility is starting to take shape... Can wait to plunge some infected cells and get to imaging! ❄️🔬🦠

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Guillaume Gaullier @guillawme.eurosky.social · 11/02/2024

"Is X-ray crystallography dead?" some asked when cryoEM got momentum. Depends on what you work on. For nucleosomes it certainly looks like it is dead: all structures with nucleosomes released in 2023 were cryoEM structures! @nucleosomepolice.bsky.social guillawme.github.io/insights-fro...

Bar graph showing the number of PDB entries containing a nucleosome released every year since 1997 (one structure). Crystal structures are red bars, cryoEM structures are green bars. In 2023, all PDB entries with a nucleosome were cryoEM structures.

(The big slow-down caused by the quarantines during the COVID-19 pandemic is also very apparent when comparing the bars for 2019 and 2020...)

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