Reposted by Benjamin Vincent
We (me and @benjamingvincent.bsky.social) naively assumed that heavy isotope labeled peptide synthesis for targeted MS was a solved problem. Wish I had read more before ordering so many contaminated peptides:
pubmed.ncbi.nlm.nih.gov/35119480/
pubmed.ncbi.nlm.nih.gov
Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics - PubMed
In mass spectrometry-based proteomics, heavy internal standards are used to validate target peptide detections and to calibrate peptide quantitation. Here, we report light contamination present in hea...
![There is a “centre” where the money, the fame, is; most likely your proposal gets funded because it’s on the most favourable topic. Maybe today, RNA is [most favourable]. If you are working with mRNA, maybe that's the centre there.
And then there are people in the periphery. There is no fame, there is no money, no nothing there. The only thing in the periphery is freedom. You can do what you like to do, what you feel is important.
Here’s what a proposal is: why they should give me money. And they should question that. “She came from university nobody knew about.” “She never had a mentor who was famous.”
And somehow it gravitates always to the same people, same circle. They get published there, they get the money. And that's another explanation: I was not famous enough or didn't have anybody who would support me in a way that somebody that’s a famous and well-established scientist stands behind you and says, “Oh, look at this, it’s good.”](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:h6srr75ahvugj6gts7ql4cpl/bafkreie7jkxonqwgbbcuni4dh54nhodteq24wv4dp6smnvsomdpwpve4we)
