iiinova — where your digital self keeps evolving

Reposted by Andrea Caldarola

Felipe Opazo @opazo.bsky.social · 04/09/2026

This new paper 🥂, just published in ACS Nano @pubs.acs.org s.acs.org, needed physicists, chemists, and biochemists in the same room! 🤓 Running out of colors in your #microscopy experiments? We developed #NanoFLex, which encodes protein identity by leveraging #nanobodies and #fluorescence lifetime

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Andrea Caldarola @andreacaldarola.bsky.social · 10/07/2026

For #FluorescenceFriday: hungry hungry HeLas get some food making them very very very happy, visualized through a very bright PIP3 sensor (PIP3 is produced in response to happiness)

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Reposted by Andrea Caldarola

bioRxiv Neuroscience @biorxiv-neursci.bsky.social · 03/06/2026

A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo www.biorxiv.org/content/10.64898/20…

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Reposted by Andrea Caldarola

bioRxiv Cell Biology @biorxiv-cellbio.bsky.social · 23/05/2026

Optogenetic cross-linking of the actin cytoskeleton using DARPins www.biorxiv.org/content/10.64898/20…

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Reposted by Andrea Caldarola

bioRxiv Neuroscience @biorxiv-neursci.bsky.social · 05/05/2026

Automated behavioral segmentation and markerless pose tracking of mice during spaceflight www.biorxiv.org/content/10.64898/20…

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Reposted by Andrea Caldarola

Chemical Science @chemicalscience.rsc.org · 15/04/2026

🔥 New and HOT in Chemical Science! 🔥 “Molecular jackhammers induce intracellular calcium release and skeletal muscle contraction by vibronic-driven action” by Anatoly Kolomeisky, Jacob T. Robinson, James M. Tour et al. from Rice University. Read it for free: doi.org/10.1039/D5SC...

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Reposted by Andrea Caldarola

Mohammed AlQuraishi @moalquraishi.bsky.social · 13/03/2026

New OpenFold3 preview out! (OF3p2) It closes the gap to AlphaFold3 for most modalities. Most critically, we're releasing everything, including training sets & configs, making OF3p2 the only current AF3-based model that is functionally trainable & reproducible from scratch🧵1/9

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Spencer LaVere Smith @spencerlaveresmith.bsky.social · 27/02/2026

Two-photon calcium imaging at 24,000 lines/s, with the resonant axis spanning 4x what other systems can do. Inertia-free. Diffraction-limited. No tradeoffs. Che-Hang Yu developed a 4x angle multiplier for laser scanning. His paper is out today: opg.optica.org/optica/fullt... 1/n #fluorescenceFriday

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Reposted by Andrea Caldarola

Claus Wilke @clauswilke.com · 13/02/2026

AI agent goes nuts on open source maintainer after having its pull request denied. This is a pretty insane story. Open source development as we've been used to for the last few decades is likely over. 1/ theshamblog.com/an-ai-agent-...

theshamblog.com

An AI Agent Published a Hit Piece on Me

Summary: An AI agent of unknown ownership autonomously wrote and published a personalized hit piece about me after I rejected its code, attempting to damage my reputation and shame me into acceptin…

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Reposted by Andrea Caldarola

I'm Uni :3 @unicouniuni3.bsky.social · 30/12/2025

He’s purring happily… but his butt is extremely close to my face 😂

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Reposted by Andrea Caldarola

Heilemann Lab @heilemannlab.bsky.social · 20/12/2025

Receptor tyrosine kinases bind ligands and (often) dimerize. Yet how long, and how does that change their mobility? Using single-molecule FRET in living cells, we found that MET dimers 'socialize' for ~1 second while lowering lateral diffusion and confinement 👉🏻 doi.org/10.1002/smll...

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Reposted by Andrea Caldarola

Joey Tidei @j-today.bsky.social · 19/12/2025

I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

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Luc Reym⌬nd @lreymond.bsky.social · 16/12/2025

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

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Ewan Birney @ewanbirney.bsky.social · 26/10/2025

I am genuinely impressed by large language models - they can absorb disparate components of text into some consolidated view, they can produce extremely good language and - with the right model - translate pretty well between languages and they are an excellent text based UI for humans to use. But..

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Reposted by Andrea Caldarola

Claire Deo @clairedeo.bsky.social · 25/10/2025

Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....

onlinelibrary.wiley.com

A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells

Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....

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Reposted by Andrea Caldarola

Kai Murk @kaimurk.bsky.social · 12/10/2025

This #microscopymonday is a cleaning day. Watch how a #microglia cell mops up and phagozytoses debris and junk in a very messy neuronal co-culture. BTW, it was a proper cleaning job → the neurons made it and formed nice networks Phase contrast imaging started at DIV1. One frame per 20 min.

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bioRxiv Cell Biology @biorxiv-cellbio.bsky.social · 12/10/2025

Optogenetic control of PLC-γ1 activity polarizes cell motility www.biorxiv.org/content/10.1101/202…

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Andrea Caldarola @andreacaldarola.bsky.social · 10/10/2025

For #FluorescenceFriday : 3 cells start at the same Ca2+ concentration but undergo 3 different Histamine responses. Imaged using the lifetime-based sensor Tq-Ca-FLITS.

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Reposted by Andrea Caldarola

bioRxiv Cell Biology @biorxiv-cellbio.bsky.social · 09/10/2025

Optogenetic construction of de novo integrin-adhesion complexes reveals role for biocondensation in adhesion nucleation www.biorxiv.org/content/10.1101/202…

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Andrea Caldarola @andreacaldarola.bsky.social · 30/09/2025

“We are making sure that people have a stronger bond with our Kingdom and are well integrated and able to fully participate in society before becoming Dutch citizens” : Dutch society is obviously too alien & bizarre for a silly foreigner to integrate within 5 years, we'll take your tax money though!

nltimes.nl

Cabinet to make 10 years minimum to obtain Dutch citizenship

According to the government, the Dutch cabinet plans to double the minimum residency requirement for naturalization from five to 10 years, extending the time foreigners must live legally and continuou...

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Reposted by Andrea Caldarola

Rakyan Lab @rakyanlab.bsky.social · 17/07/2025

Paper in Nature 150 years ago. Beats reading any single-cell experiment…

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Andrea Caldarola @andreacaldarola.bsky.social · 25/06/2025

Also a great supervisor!! In my entirely unbiased opinion

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Reposted by Andrea Caldarola

Robert E. Campbell @campbell-lab.bsky.social · 19/04/2025

New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...

biorxiv.org

High-performance genetically-encoded green and red fluorescent biosensors for pyruvate

Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...

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Reposted by Andrea Caldarola

spooky cannoli @boner.ink · 09/04/2025

president tweeted "no hot dog" and i lost $5 trillion dollars but today he tweet "hot dog back" and i got 5 trillion hot dog

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Adam Glaser @adamkglaser.bsky.social · 04/04/2025

Sound-sheet microscopy - in this study, we introduce nonlinear sound-sheet microscopy (NSSM), a method to image targeted biological functions across cubic centimeters of opaque living tissue. www.science.org/doi/10.1126/...

science.org

Nonlinear sound-sheet microscopy: Imaging opaque organs at the capillary and cellular scale

Light-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescen...

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Reposted by Andrea Caldarola

Biology Open @biologyopen.bsky.social · 25/03/2025

We are excited to be launching the next phase of our Fast & Fair peer review initiative: offering high-quality peer review within 7 working days. #fastandfairpeerreview Read the Editorial by EiC Daniel Gorelick @danielgorelick.bsky.social at: bit.ly/4kYD1mL

Fast & Fair peer review

A new initiative offering high-quality peer review within 7 working days of submission

Biology Open

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Anh Hoang Le, PhD 🏳️‍🌈🏴󠁧󠁢󠁳󠁣󠁴󠁿🇻🇳 @anhhle2702.bsky.social · 14/03/2025

So happy I finally made the ILID-SspB Optogenetic system work in 🐸. Though not perfect and need some optimisations, it worked. Got the constructs from @joachimgoedhart.bsky.social and made it into a frog expression system. Here is the Opto-PH-Rho in action. It's real, guys!! #FluorescenceFriday

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@LaBonneLaB @labonnelab.bsky.social · 13/03/2025

Dear all, Because of your help shining light on this all 15 were reinstated today. This is a huge win for science - When We Fight We Win!!!

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Richard Lincoln, PhD @rlincolnchemist.bsky.social · 28/02/2025

📢 #Preprint Alert: For #fluorescentfriday we report minimally-sized photoactivatable PaX dyes with red and far-red emission for #STED, #SMLM, and multiplexed #MINFLUX #SuperResolution #Microscopy! A huge thanks to the Optical #Nanoscopy team at @mpi-mr.bsky.social! 🔬 www.biorxiv.org/content/10.1...

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Arjun Raj @arjunraj.bsky.social · 26/02/2025

Gen Z from lab, reflecting wistfully upon the passage of time in grad school: “dang rip”

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Reposted by Andrea Caldarola

Stephen Curry @scurry.bsky.social · 23/02/2025

A million times the man that Trump or Putin will ever be.

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