iiinova — where your digital self keeps evolving

Reposted by Tanner Fadero

Chad Hobson @cmhobson.bsky.social · 27/08/2026

A couple years in the making, but the preprint is finally out! We developed a pipeline for timelapse, volumetric FLIM (vFLIM) that incorporates multi-channel light sheet excitation, a SPAD array detector for widefield FLIM, and machine learning lifetime processing. www.biorxiv.org/content/10.6...

biorxiv.org

vFLIM: Machine Learning-enabled Light Sheet Fluorescence Lifetime Imaging

The lifetime of fluorescent molecules provides an orthogonal readout to fluorescence intensity, opening experimental possibilities of measuring changes in local molecular environments, mechanical tens...

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Tanner Fadero @tanner-fadero.bsky.social · 13/08/2026

Where I can get a custom Zernike phase plate fabricated? I need a glass window with a ~100 um diameter hole etched down to a depth of ~250 nm. I also need a window with a reflective ND coating in a ~100 um diameter spot. @retof.bsky.social @amsikking.bsky.social @kmdouglass.bsky.social

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Reposted by Tanner Fadero

Jon Daniels @jsdaniel02.bsky.social · 28/07/2026

Yes I'll make sure the specs get added to the store, and also that we make a nice landing page. Below are the tabulated specs. Note that if you don't need the full design FOV then a larger aperture can usually be supported and vice versa. Black box files available.

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Tanner Fadero @tanner-fadero.bsky.social · 27/07/2026

ASI has begun offering large aperture tube lenses! Really useful for large-FOV imaging at a variety of focal lengths. The large aperture is also useful if you need to put the tube lens in a 4f-configuration without clipping! @jsdaniel02.bsky.social www.asiimaging.com/product/sm2-...

asiimaging.com

SM2-TUBE-200C: Plan-corrected tube lens assembly with 200 mm focal length and SM2 interface – ASI

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Reposted by Tanner Fadero

Alfred Millett-Sikking @amsikking.bsky.social · 20/07/2026

Ultrafast 3D imaging just got faster! Introducing: "Instant dual-view Snouty (iDV-Snouty) light-sheet microscopy" -> Double the data rate of a Snouty microscope by adding a 2nd view! Use the extra view for a variety of imaging modes! Article here: amsikking.github.io/idv-snouty_l...

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Tanner Fadero @tanner-fadero.bsky.social · 27/06/2026

Is @biorxivpreprint.bsky.social inaccessible for everyone, or just me? It's on every device and every browser that I use, whether over wifi or over cellular data. Even my spouse's devices, who has never accessed Biorxiv before today.

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Reposted by Tanner Fadero

Reese Richardson @reeserichardson.bsky.social · 05/06/2026

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

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Ali Shaib @alishaib.bsky.social · 01/06/2026

Hello #world, meet 1,000× Expansion Microscopy. A small gel would grow to the size of an Olympic swimming pool, while amino-acid-scale distances become visible with ordinary light microscopy. Led by Helena Hu from @eboyden3.bsky.social's lab, in collab with us. Story: www.biorxiv.org/content/10.6...

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Tanner Fadero @tanner-fadero.bsky.social · 29/05/2026

Plasmodium sporozites (green) moving through and deforming a fibrin matrix (red), imaged on a #snouty light sheet by Rachel Stadler and @joseph-brzostowski.bsky.social. #fluorescencefriday

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Reposted by Tanner Fadero

Nico Stuurman @nicostuurman.bsky.social · 26/05/2026

Because @tanner-fadero.bsky.social asked for it and I had tokens to spare, I built an orthogonal viewer for @micro-manager.bsky.social today.

Screenshot of a viewer showing xy, xz, and yz planes of a 3D dataset.

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micro-manager.bsky.social @micro-manager.bsky.social · 21/05/2026

Development of Micro-Manager code has accelerated quite a bit with availability of the new generation of AI coding tools (and the need to provide functionality for microscopes in our core at UCSF). Follow these developments here: nicost.github.io/microscope-t...

nicost.github.io

Half a year of progress in Micro-Manager development

Faster progress on Micro-Manager development thanks to new AI coding tools

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Torsten Wittmann @microtubule.bsky.social · 22/05/2026

A day in the life of a growing hiPSC spheroid expressing endogenously mStayGold-tagged histone 3.3 for #FluorescenceFriday and the #Snouty aficionados. 28 hours (and >50,000 exposures) later and they are still going! Home-brewed volume projection.

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NPR @npr.org · 21/05/2026

Even with federal grants largely restored, scientists say the Trump administration is still preventing those funds from reaching them. The consequences, they say, are already becoming clear. n.pr/4dwU7Fi

n.pr

Researchers say the Trump administration is finding new ways to punish science

Even with federal grants largely restored, scientists say the Trump administration is still preventing those funds from reaching them. The consequences, they say, are already becoming clear.

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Reposted by Tanner Fadero

Julia Lazzari-Dean @jlazzaridean.bsky.social · 12/05/2026

We invented “triplet tumbling microscopy” (TTM) to track protein-protein interactions in living cells! Tag your protein with GFP and see it interact with *untagged* binding partners www.biorxiv.org/content/10.6...

A cartoon overview of triplet tumbling microscopy, comparing data from bound and unbound samples.

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Kevin Dean @kevin-dean.bsky.social · 12/05/2026

Altair Direct View OPM Preprint! We built a simplified direct-view oblique-plane microscope for imaging big, optically accessible specimens with a single-objective light-sheet geometry. Delivers... • ~5.4 mm field of view • ~2.6 μm, ~3.4 μm, and ~17.8 μm resolution in X/Y/Z

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Andrew G. York @andrewgyork.bsky.social · 29/04/2026

Meet MagLuc: a magnetically-controlled luminescent protein. This is a foundational tool for remote control of biological processes, also known as "magnetogenetics". Can you tell when the magnetic field turns on and off? From @mariaingaramo.bsky.social and the team at @nonfictionlabs.bsky.social

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Tanner Fadero @tanner-fadero.bsky.social · 25/04/2026

Besides the clearly visible image duplication and precognitive mice that can predict future perturbations, they make some truly EXTRAORDINARY claims that are EASILY replicatable. They applied 60 Hz EMF with 4 kHz modulation to WILD-TYPE fibroblast cells and saw rhythmic calcium fluctuations.

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Tanner Fadero @tanner-fadero.bsky.social · 10/03/2026

@patrickzager.bsky.social acquired this lovely 3D timelapse of live neutrophil membrane protrusions on the snoutscope at the UCSF Center for Advanced Light Microscopy. Shown are three orthogonal maximum intensity projections.

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Reposted by Tanner Fadero

Reto Fiolka @retof.bsky.social · 30/01/2026

Integrated in an imaging system with a 60X NA 1.49 lens. Pretty clean rotation near optical axis.

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Tanner Fadero @tanner-fadero.bsky.social · 26/01/2026

There are some things that blue lasers can't excite... ... for everything else, there's red and orange lasers

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Reposted by Tanner Fadero

Nature @nature.com · 21/01/2026

Researchers have engineered magnetically controlled fluorescent proteins that can be remotely dimmed and brightened in cells and living animals go.nature.com/3NUSY12

go.nature.com

‘Remote controlled’ proteins illuminate living cells

The discovery that some fluorescent proteins are sensitive to magnets could lead to the development of switchable drugs and biosensors.

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Andrew G. York @andrewgyork.bsky.social · 13/01/2026

Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody. Its binding turns on and off with a magnet. This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.

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Hamamatsu Corporation @hamamatsucorp.bsky.social · 25/11/2025

We’re thrilled to feature Dr. Tanner Fadero from the Chan Zuckerberg Imaging Institute in an upcoming Nature-hosted webinar about... PHOTONS! 😎 Join the webinar to learn and take part in the live Q&A. 👉 Register here: ow.ly/vsyn50XkZ88 #FluorescenceMicroscopy #LightSheetMicroscopy

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Tanner Fadero @tanner-fadero.bsky.social · 19/11/2025

Motorized slit! Off-the-shelf part from ScienceTech. Thorlabs stepper motor driven by K-cube controller. www.sciencetech-inc.com/shop/product... @amsikking.bsky.social @retof.bsky.social @kevin-dean.bsky.social

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Torsten Wittmann @microtubule.bsky.social · 08/10/2025

Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.

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Kari Herrington @kahsage.bsky.social · 18/09/2025

@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.

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Alfred Millett-Sikking @amsikking.bsky.social · 01/09/2025

What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post). -> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!

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Reposted by Tanner Fadero

Torsten Wittmann @microtubule.bsky.social · 29/08/2025

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

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Tanner Fadero @tanner-fadero.bsky.social · 28/08/2025

Optics folks: does anyone know a manufacturer of a dichroic beamsplitter *cube*? Essentially, a dichroic version of this (for fluorescence microscopy):

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Tanner Fadero @tanner-fadero.bsky.social · 21/08/2025

Science hive mind! Does anyone do hardware tech dev on MRI or CT? Or do you know someone who does? I'd love an introduction, if so. Let me know!

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Tanner Fadero @tanner-fadero.bsky.social · 20/08/2025

Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy! Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social. #snouty

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Tanner Fadero @tanner-fadero.bsky.social · 19/08/2025

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

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Tanner Fadero @tanner-fadero.bsky.social · 05/08/2025

Hey @thorlabs.bsky.social! Thanks for such a well-documented APT Controller Communication Protocol! It was fun coding against it. @andrewgyork.bsky.social and I did find a small bug, though. When talking to a MFF102 flipper, it only ever reports clockwise motion (never counterclockwise).

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Tanner Fadero @tanner-fadero.bsky.social · 26/07/2025

If you've ever wanted to know what the actin filaments on the scales in a developing moth wing look like in 3D, here you go! Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy. #snouty

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Torsten Wittmann @microtubule.bsky.social · 25/07/2025

Finally having some time to play with ‘Snouty’ again. Thanks a million once more @tanner-fadero.bsky.social and @amsikking.bsky.social

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DrLachie @drlachie.bsky.social · 10/07/2025

Assume it's still done this way? www.youtube.com/watch?v=2s0O...

youtube.com

The making of "Mr Snouty"

YouTube video by Microscopist's Nightmares

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Reposted by Tanner Fadero

Jon Daniels @jsdaniel02.bsky.social · 09/07/2025

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

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Tanner Fadero @tanner-fadero.bsky.social · 01/07/2025

I spent some time finally deriving the relationship between lateral image magnification and axial magnification! It's an interesting equation, with dependencies on sample refractive index and the wave propogation angle w.r.t. the optical axis. Play with it yourself! www.desmos.com/calculator/q...

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Tanner Fadero @tanner-fadero.bsky.social · 26/06/2025

@kahsage.bsky.social is killing it with these teaching tools for #snoutscope training at the UCSF Center for Advanced Light Microscopy. Fantastic illustrations and a whiteboard in the room. Brilliant!

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Joachim Goedhart @joachimgoedhart.bsky.social · 26/06/2025

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

$Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.$

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Will Ratcliff @wcratcliff.bsky.social · 24/06/2025

1/27 We have a new paper out! Turns out that snowflake yeast have been hiding a secret from us - they've evolved a (very!) crude circulatory system. Not with blood vessels or a heart, but through spontaneous fluid flows powered by their metabolism. 🧪🔬 www.science.org/doi/full/10....

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Tanner Fadero @tanner-fadero.bsky.social · 24/06/2025

Another #snoutscope imaging session at the UCSF Center for Advanced Light Microscopy! This time, with neutrophils! Courtesy of Patrick Zager in the Weiner lab. Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.

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Tanner Fadero @tanner-fadero.bsky.social · 21/06/2025

I got a text from Yovan Bodal, who just used our #snoutscope @UCSF to image fly embryos. His quote says it all! "This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"

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