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Jon Daniels @jsdaniel02.bsky.social · 29/09/2026

Glad that you found a solution for striping with the ASI single-objective microscope!

Jon Daniels @jsdaniel02.bsky.social · 21/02/2026

Gratifying to have been a part of the tech dev behind this (specifically high-res cleared tissue light sheet microscopy)

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Jon Daniels @jsdaniel02.bsky.social · 20/02/2026

I'm very happy with the new ExM objective design that I've been working on with Special Optics Now looking for pre-orders using kickstarter model. Key specs: - NA 1.0 water-dipping - FOV 1.1 mm - WD 5.4 mm - EFL 7.5 mm - flat field - chromatic corrections optimized for 500-750 nm

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Jon Daniels @jsdaniel02.bsky.social · 17/02/2026

Cool use for the ASI single-objective light sheet at MPI-CBG

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Jon Daniels @jsdaniel02.bsky.social · 22/01/2026

I'm working on an objective lens for expansion microscopy. I have a candidate design to share. Looking for feedback before we finalize things. NA 1.0 water-dipping 5.5mm WD 1.1mm FOV, plan-corrected 8mm EFL ("25x") corrected in visible with exception noted below minimum mechanical profile

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Jon Daniels @jsdaniel02.bsky.social · 22/01/2026

I highly recommend this course as an introduction to microscopy.

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Reposted by Jon Daniels

Neuronal Signalling Lab @mjc-lab.bsky.social · 19/01/2026

Our snouty v3 (AMS-AGY v3) objective arrived today! Looking forward to getting it installed on our single objective light sheet system very soon...

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Jon Daniels @jsdaniel02.bsky.social · 18/12/2025

I'm working on a new water-dipping objective targeting expansion imaging. Targeting the following specs: 5mm WD NA 1.0 1.2mm FOV, plan-corrected 8mm EFL ("25x") but this is flexible well-corrected in visible minimum mechanical profile Optics question: is there any reason to make it telecentric?

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Jon Daniels @jsdaniel02.bsky.social · 09/12/2025

The ASI machine shop made a nice replica model of the AMS-AGY v3 objective for us to have in the #CellBio2025 booth. The first of the real ones will be ready by the end of the year.

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Jon Daniels @jsdaniel02.bsky.social · 07/12/2025

If you are at #CellBio2025 stop by and say hello at booth 1101

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Jon Daniels @jsdaniel02.bsky.social · 09/07/2025

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

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Jon Daniels @jsdaniel02.bsky.social · 15/05/2025

I'm excited to be able to be in Zurich for the mesoSPIM symposium. This is a really fantastic project.

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Jon Daniels @jsdaniel02.bsky.social · 14/03/2025

Most high-NA air objective lenses are coverslip-corrected but sometimes you want to image in air, e.g. high-NA SOLS. We have glued coverslips onto objectives for this. To make sure it gets glued properly we take image stack of diffraction-limited beads supported on a coverslip. 🧵

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Jon Daniels @jsdaniel02.bsky.social · 06/12/2024

Interested in getting imaging data like this? ASI is commercializing single-objective light sheet microscopes and might make one like DaXi if there are people who would buy it. DaXi originally developed by @loicaroyer.bsky.social and Bin Yang. vimeo.com/801341925

vimeo.com

Zebrafish embryo development acquired on the DaXi light-sheet microscope

Volumetric imaging of zebrafish embryo development acquired on the DaXi light-sheet microscope. Imaging volume is 1200 μm * 800 μm * 300 μm acquired every…

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