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Spirochrome

@spirochrome.com
2.3K followers 4.1K following 115 posts

Our main focus is to offer innovative tools for live cell fluorescence imaging to the scientific community. spirochrome.com

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Reposted by Spirochrome
Juanma García-Arcos @juanmagararc.bsky.social · 10/09/2026
1/3 A few snapshots from the past intense days at the University of Helsinki for our FLIM and FCS course! We combined lectures, hands-on microscopy and analysis with plenty of questions and discussion. A fantastic group of researchers to learn with. 🔬 #FLIM #FCS #Microscopy
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🍁 Bryan Heit, PhD 🍁 @bryanheit.bsky.social · 28/08/2026
Happy #FluorescenceFriday! This is the cortical actin cytoskeleton of a macrophage imaged using eSRRF and @spirochrome.com's SPY555-FastAct_X.
image shows the web-like network formed by actin. Image is pseudo-coloured using a heatmap with red "dim" regions and orange-white "bright" regions.
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Spirochrome @spirochrome.com · 01/06/2026
Spoiler: lots of 😍 SPY555-FastAct live cell movies in there.
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Spirochrome @spirochrome.com · 01/06/2026
And it's not all: For an exceptional new product, an exceptional new casing! We designed new boxes that we will gradually deploy to replace our old metal boxes. 4 designs will be randomly used for each product!
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Spirochrome @spirochrome.com · 01/06/2026
Today is the Day, CenSpark650 is available! Time to get those centrioles and cilia shining under the 🔬objective! spirochrome.com/product/cens... video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650. movie credits: @cepourroy.bsky.social
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Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦ @dudinlab.bsky.social · 28/05/2026
An amazing new tool to explore "non-models" is just about to open a new world of imagining possibilities....
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Spirochrome @spirochrome.com · 27/05/2026
We strongly recommend reading the article by @cepourroy.bsky.social to get to know everything CenSpark650 can do and beyond! www.nature.com/articles/s41...
nature.com
Development of the fluorescent probe CenSpark for labeling centrioles and cilia - Nature Chemical Biology
CenSpark is a dual-ligand fluorescent probe binding simultaneously inner and outer microtubule sites, a configuration unique to doublet and triplet microtubules, enabling selective live imaging of cen...
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Spirochrome @spirochrome.com · 27/05/2026
📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/ #Bioimaging
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Spirochrome @spirochrome.com · 04/05/2026
Casually posting this one for no particular reason! #MayTheFourth
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Spirochrome @spirochrome.com · 01/05/2026
Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆 The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes. evidentscientific.com/en/landing/i...
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Luc Reym⌬nd @lreymond.bsky.social · 01/05/2026
#FluorescenceFriday material here!
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Spirochrome @spirochrome.com · 01/05/2026
If you want to know more about Induced pluripotent stem cell-derived neurospheres, we strongly advise to read Katie Holden's research article which introduces this technology: doi.org/10.1016/j.bb... Congrats Katie!
doi.org
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Spirochrome @spirochrome.com · 01/05/2026
Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆 The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes. evidentscientific.com/en/landing/i...
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Spirochrome @spirochrome.com · 21/04/2026
Congratulations! The imaging part is absolutely mind blowing 🤯
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Reposted by Spirochrome
Evelyn Strickland @strickland-evelyn.bsky.social · 20/04/2026
What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...
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Spirochrome @spirochrome.com · 21/04/2026
Mesmerizing light sheet imaging of neutrophils swarming at a yeast target! The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue). This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading! www.biorxiv.org/content/10.6...
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Spirochrome @spirochrome.com · 16/04/2026
What a probe! Congratulations @cepourroy.bsky.social! We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!
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Spirochrome @spirochrome.com · 08/04/2026
Thank you very much for sharing this cool animation!
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Spirochrome @spirochrome.com · 08/04/2026
Organoids stained with Spirochrome probes for actin and DNA! 😍
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Reposted by Spirochrome
Zhixing Chen @zhixingchen2.bsky.social · 04/04/2026
See how PKMito dyes promote mitochondrial research😀 @spirochrome.com
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Spirochrome @spirochrome.com · 01/04/2026
🙏 @christlet.bsky.social for the cool "Animated Zooming" & "Animated panning" ImageJ plugins used here 😎
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Spirochrome @spirochrome.com · 01/04/2026
Beware the SPY488-FastAct rollercoaster! We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct. Find out more about SPY488-FastAct: spirochrome.com/product/spy4...
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Spirochrome @spirochrome.com · 23/03/2026
📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy! There is now a very detailed #JOVE video that explains it all step by step. Video link: dx.doi.org/10.3791/6940... Peroxi_SPY link: spirochrome.com/products/?_c... 🧪
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Spirochrome @spirochrome.com · 23/03/2026
📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy! There is now a very detailed #JOVE video that explains it all step by step. Video link: dx.doi.org/10.3791/6940... Peroxi_SPY link: spirochrome.com/products/?_c... 🧪
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Spirochrome @spirochrome.com · 18/03/2026
🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.
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Spirochrome @spirochrome.com · 16/03/2026
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™. These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green). Movie from preprint www.biorxiv.org/content/10.6... 🧪
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Spirochrome @spirochrome.com · 16/03/2026
HAK-actin product link: spirochrome.com/product/hak-...
spirochrome.com
HAK-actin™ - Actin Expansion Microscopy Probe - Spirochrome
HAK-actin™ is a pan-species Expansion Microscopy (ExM) compatible probe for actin visualization using U-ExM based protocols
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Spirochrome @spirochrome.com · 16/03/2026
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™. These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green). Movie from preprint www.biorxiv.org/content/10.6... 🧪
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Spirochrome @spirochrome.com · 10/03/2026
🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁 Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT) Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
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Spirochrome @spirochrome.com · 10/03/2026
5/5 The Takeaway: Flipper-TR didn't just report CMT; it identified CMT as a master switch linking physics to genetics. From development to disease, this probe proved that how "tight" a cell is matters just as much as its DNA. Read the full study: www.biorxiv.org/content/10.6...
biorxiv.org
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Spirochrome @spirochrome.com · 10/03/2026
4/5 The "Stuck" State: Flipper-TR also exposed a fibrosis mechanism. In healthy lungs, tension drops to allow repair. But in fibrotic lungs (IPF), Flipper-TR showed tension stays high. This high tension state traps cells in a dysfunctional "transitional state," preventing healing.
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Spirochrome @spirochrome.com · 10/03/2026
3/5 Mapping the Invisible: Using Flipper-TR + FLIM imaging, researchers visualized tension in live lung tissue. They discovered a drop in membrane tension before cells differentiate into AT1 or AT2 alveolar cells. Even cooler? It mapped "emergent zones" of low tension where differentiation starts!
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Spirochrome @spirochrome.com · 10/03/2026
2/5 The Challenge: Scientists knew various signals guided lung differentiation, but how do cells "know" when to specialize? Here comes Flipper-TR: A mechanosensitive dye that changes its fluorescence lifetime based on CMT. High tension = Long lifetime; Low tension = Short lifetime
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Spirochrome @spirochrome.com · 10/03/2026
🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁 Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT) Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
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Spirochrome @spirochrome.com · 03/03/2026
🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪
Widefield microscope image of HeLa cells stained with SPY488-FastActWidefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)
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Spirochrome @spirochrome.com · 03/03/2026
🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪
Widefield microscope image of HeLa cells stained with SPY488-FastActWidefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)
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Gerlich Lab @gerlichlab.bsky.social · 25/02/2026
1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰ Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs: www.biorxiv.org/content/10.6...
biorxiv.org
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Spirochrome @spirochrome.com · 27/02/2026
Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday Easy to use: just dilute the probe in cell culture medium and image 30 min later! 🔗 spirochrome.com/product/spy6... 🙏 Big thanks to @lazaresaladin.bsky.social for the images. 🧪
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Spirochrome @spirochrome.com · 27/02/2026
Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday Easy to use: just dilute the probe in cell culture medium and image 30 min later! 🔗 spirochrome.com/product/spy6... 🙏 Big thanks to @lazaresaladin.bsky.social for the images. 🧪
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Spirochrome @spirochrome.com · 27/02/2026
We love how amazing our SPY650-DNA probe looks on your microscopy images. Congratulations to everyone involved!
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Spirochrome @spirochrome.com · 27/02/2026
An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list! Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript. Congratulations to the team!
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Joey Tidei @j-today.bsky.social · 24/02/2026
SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!
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Spirochrome @spirochrome.com · 25/02/2026
Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X. 🤩
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Spirochrome @spirochrome.com · 23/02/2026
ICYMI SPY555-FastAct_X and SiR-tubulin in action here! 🤯 16h imaging of neurons 💥 Thank you @j-today.bsky.social for posting this gem!
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Spirochrome @spirochrome.com · 23/02/2026
Amazing movie! We love to see our probes SPY555-FastAct_X and Sir-tubulin perform so well in your hands! 🤩 Thank you for sharing.
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epithelial mechanics fan club @epimechfc.bsky.social · 08/02/2026
Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells. Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!
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Spirochrome @spirochrome.com · 23/01/2026
Thank you Kim for your amazing work! We love your designs and working with you on the HAK-actin™ project was fast, efficient and a real pleasure!
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Kim Gaudin @gaudinkim.bsky.social · 20/01/2026
📐 Visual communication for @spirochrome.com Design for the launch of HAK-actin™, a new fluorescent probe for Expansion Microscopy. Project included a clear usage schematic, flyer, and folded leaflet, with a minimalist, efficient style aligned with Spirochrome’s visual identity.
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Spirochrome @spirochrome.com · 09/01/2026
You are correct! mtDNA staining is typically performed with an intercalating DNA dye with nanomolar affinity for dsDNA. SPY-DNA probes, primarily designed for live cell imaging, are DNA groove binders and have a lower affinity to avoid toxicity. Probably not strong enough for robust mtDNA staining.
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Spirochrome @spirochrome.com · 08/01/2026
The images were acquired on a @leicamicrosystems.bsky.social Stellaris system by @bioarm.bsky.social from @centriolelab.bsky.social. Huge thank you for trying this out!
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