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Sila Sopran

@silasopran.bsky.social
524 followers 875 following 3 posts

SynBio lover. 🧬🌻 Biotech student Py 🇵🇾

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Reposted by Sila Sopran
Philip Ball @philipcball.bsky.social · 16/10/2026
This is a truly startling paper. It shows that mRNA can perform regulatory functions *in addition to* its role in encoding a protein. In other words, the distinction between protein-coding RNA and noncoding regulatory RNA is fuzzy. /1 www.science.org/doi/10.1126/...
science.org
Regulation of target gene expression by chromatin-associated protein-coding transcripts in plants
Protein-coding transcripts are regarded as intermediates that mediate DNA-to-protein information flow, leaving their noncoding regulatory potential largely underappreciated. Genome-wide profiling of R...
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Reposted by Sila Sopran
James Briscoe @jamesbriscoe.bsky.social · 16/10/2026
New commentary from the lab - can we identify principles of transcriptional regulation & cis-regulatory element (CRE) control of gene expression? With @jcornwallscoones.bsky.social & @joadelas.bsky.social in @cp-molcell.bsky.social A thread 🧵 doi.org/10.1016/j.mo...
doi.org
Redirecting
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Benjamin Swedlund @benswedlund.bsky.social · 27/05/2026
I’m super excited to announce that the work from my postdoc is now openly available on BioRxiv!! A novel reaction-diffusion architecture for engineering self-organized patterns in mammalian cells, from @leonardomorsut.bsky.social lab and friends (1/9) www.biorxiv.org/content/10.6...
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Nicholas Provart @bar-plantbio.bsky.social · 24/09/2026
Check out the BAR's Single Cell Data Portal at bar.utoronto.ca/single_cell_..., in beta. There's a curated list of scRNA-seq datasets with viewer links, our own CellxGene instance for exploring 14 datasets from 6 papers, and a new tool, SUPeR Viewer! Thanks to PCA and C-SPIRIT for input.
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Bas Bargmann @bas-bargmann.bsky.social · 25/09/2026
To visualize the development of protoplast regeneration microscopically with enhanced detail and resolution, we incorporate fluorescent cellular markers for the plasma membrane and the nucleus. To better distinguish cell-division events, which can be challenging with brightfield microscopy. (5/n)
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epithelial mechanics fan club @epimechfc.bsky.social · 27/09/2026
Can mathematics explain how biological patterns are formed? Alan Turing thought so. Join me, @benswedlund.bsky.social, as we explore his reaction-diffusion theory and how it transformed our understanding of pattern formation and self-organisation.
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Chenxin Li, PhD @chenxinli2.bsky.social · 20/09/2026
Single cell transcriptome reveals the molecular pathways of MADS-box genes in maize ovule development From: academic.oup.com/plcell/advan...
academic.oup.com
Single cell transcriptome reveals the molecular pathways of MADS-box genes in maize ovule development
Abstract. Flowers are the reproductive structures of flowering plants, involved in seed production. A mature flower has four whorls of specialized organs t
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Laurens Pauwels @laurenspauwels.bsky.social · 12/09/2026
BackBone Builder (B3): a modular Golden Gate standard with a compatible Agrobacterium parts library is now published in @plantphys.bsky.social ! Has been a very useful system for our lab. @psb-vib.bsky.social #PlantScience academic.oup.com/plphys/artic...
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Nature Plants @natplants.nature.com · 08/09/2026
New Brief Communication: "Cell-based CRISPRa screen identifies a papillae-specific receptor kinase mediating broad-spectrum fungal resistance in rice" rdcu.be/ZZFv8dTuRi8a A RK that positively regulates fungal resistance via silicon-mediated physical & biochemical defences. #PlantScience
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Reposted by Sila Sopran
Arvid Herrmann 🍃 @arvidhema.bsky.social · 08/09/2026
Very happy to see this work out as a preprint! 🌽🌱 How are shoot stem cells specified in the maize embryo? We uncover a small RNA-driven morphogenic circuit linking positional information with auxin, cell wall mechanics & cell division patterning. Check it out 👇 www.biorxiv.org/content/10.6...
biorxiv.org
Specification of embryonic shoot stem cells via a small RNA-driven morphogenic circuit
The specification of embryonic stem cells capable of self-renewing and differentiation into virtually any cell type, is one of the most consequential events in the development of a multicellular organ...
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Tatsuya Nobori @tatsuyanobori.bsky.social · 03/09/2026
Great work by Ingram & Zipfel labs. Congratulations, everyone! An embryo-derived peptide signal directs endosperm polarity in Arabidopsis www.science.org/doi/10.1126/...
science.org
An embryo-derived peptide signal directs endosperm polarity in Arabidopsis
Angiosperm seed formation requires the coordinated development of the products of double fertilization—the embryo and the endosperm. The endosperm mediates efficient nutrient transfer from surrounding...
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James Lloyd 🧬 @jamespblloyd.bsky.social · 25/08/2026
Introducing PICARD-seq: might take on using ONT MinION to rapidly sequence large complex plasmids in-house. dx.doi.org/10.17504/pro... 1/5
dx.doi.org
Plasmid Integrity by Complete Assembly and Rapid Determination sequencing (PICARD-seq) using ONT MinION
Plasmid Integrity by Complete Assembly and Rapid Determination sequencing (PICARD-seq) using ONT MinION. . Read full protocol, steps, and materials on protoc...
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Chenxin Li, PhD @chenxinli2.bsky.social · 28/08/2026
3 new GitHub repositories from me! I put what I'm teaching on GitHub. Module 1: github.com/cxli233/Sect... Module 2: github.com/cxli233/Sect... Module 3: github.com/cxli233/Sect... #Rstats #Datavis
github.com
GitHub - cxli233/Section-301-R-Programming-Intro: R programming foundations for bioinformatics, data science, and data visualization
R programming foundations for bioinformatics, data science, and data visualization - cxli233/Section-301-R-Programming-Intro
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Akira Yoshinari @ayoshinari.bsky.social · 22/08/2026
We’re thrilled to announce that our new preprint is now live! In this study, we uncover a previously unrecognized molecular framework underlying radial cell polarity in plants. www.biorxiv.org/content/10.6...
biorxiv.org
The Lateral Protein Cluster as a Key Component of Plant Cell Polarity
Cell polarity is an ancient organizing principle across kingdoms. As in animal epithelial cells, plant cells asymmetrically distribute proteins to establish functionally distinct membrane domains. In ...
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bioRxiv Plant Bio @biorxiv-plants.bsky.social · 24/08/2026
Specification of embryonic shoot stem cells via a small RNA-driven morphogenic circuit www.biorxiv.org/content/10.64898/20…
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Stephen Turner @stephenturner.us · 13/08/2026
Coolest paper I've seen in a while. Female mice created from male embryos ♂️♀️ www.biorxiv.org/content/10.6...
biorxiv.org
Initiating mammalian reproduction from a single male genome
Sex in mammals is unidirectionally determined by the configuration of sex chromosomes. In contrast, several species, including fishes, can switch sexes in response to environmental cues, illustrating ...
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Reposted by Sila Sopran
Tatsuya Nobori @tatsuyanobori.bsky.social · 30/07/2026
A collection of perspectives on the opportunities and challenges in single-cell and spatial biology is now published in @ThePlantCell . academic.oup.com/plcell/advan... Happy to have contributed the chapter, “Spatial transcriptomics in plants: where next?”
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Darius Kosmützky @dariuskos.bsky.social · 31/07/2026
Ever had issues with contamination in #Marchantia or #liverwort tissue culture? We've developed a protocol that is simple and low-effort (albeit slow) to get rid of fungi and bacteria: dx.doi.org/10.17504/pro.... Work led by Isaac Coven and supported by Chetan Pandey, myself & @dromius.bsky.social.
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Plant Systems Biology @psb-vib.bsky.social · 30/06/2026
PAPER from @therhizolab.bsky.social, @vandepoelelab.bsky.social, @bertderybel.bsky.social in @currentbiology.bsky.social www.cell.com/current-biol...
cell.com
Decoding stage-specific symbiotic programs in the Rhizophagus irregularis-tomato interaction using single-nucleus transcriptomics
Stuer et al. use single-nucleus RNA-seq to resolve stage-specific transcriptional programs during arbuscular mycorrhizal colonization of tomato roots. Their analysis identifies candidate transcription...
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Felipe Opazo @opazo.bsky.social · 02/07/2026
New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚
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Plant & Cell Physiology @plantcellphysiol.bsky.social · 25/06/2026
Plasticity & robustness through a 3D lens 🔎 Suzuki & Ueda comment on the work by Tezuka et al. (2026) demonstrating the remarkable plasticity & robustness shown by early #rice embryo cells during establishment of the apical−basal & inner−outer axes doi.org/10.1093/pcp/... #PlantScience
Overview of rice embryogenesis and geometric principles underlying cell division, taken from Figure 1 of Hidemasa Suzuki, Minako Ueda, Plant embryogenesis: plasticity and robustness through a 3D lens, Plant and Cell Physiology, Volume 67, Issue 5, May 2026, Pages 701–703, https://doi.org/10.1093/pcp/pcag029
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Chenxin Li, PhD @chenxinli2.bsky.social · 23/06/2026
Interesting paper: Somatic embryogensis, like normal embryogenesis, resets the epigenetic state of vernalization. On the other hand, regeneration from callus does not reset that. #PlantScience From: www.nature.com/articles/s41...
nature.com
Resetting of epigenetic cold memory through somatic embryogenesis in plant regeneration - Nature Plants
This study shows that the epigenetic cold memory or ‘vernalized state’ in overwintering plants can be reset through somatic embryogenesis, overturning the long-standing idea that the vernalized state ...
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Plant & Cell Physiology @plantcellphysiol.bsky.social · 12/05/2026
A comprehensive database for #Marchantia research Tanizawa et al. present the new improved MarpolBase #database - a central resource for M. polymorpha #genomics with near-telomere-to-telomere reference male Tak-1 and female Tak-2 genomes (ver. 7.1) 🆓 doi.org/10.1093/pcp/... #PlantScience
marchantia.info
[Important notice] To maintain nomenclature consistent and systematic, we strongly recommend that gene names/symbols should be registered before publications and presentations.
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Gitta Coaker @gittacoaker.bsky.social · 12/06/2026
Marchantia has two RBOHs. Double mutant = disorganized cell mass. Conditional mutant analyses show they are required to sustain morphogenesis via cuticle-cell-wall integrity. Clever study. www.sciencedirect.com/science/arti...
sciencedirect.com
Indispensable roles of RBOH-mediated ROS production in development and morphogenesis of Marchantia polymorpha
Reactive oxygen species (ROS) are now well established as essential signaling molecules in eukaryotes. In plants, NADPH oxidases, known as respiratory…
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The Sainsbury Laboratory @thesainsburylab.bsky.social · 11/06/2026
TSL Synbio just deposited 192 highly requested plasmids on @addgene.bsky.social! 🧪 Popular by demand: Our deposits include MoClo Plant promoters, plant terminators, C-terminal and N-terminal tags. Find them here: www.addgene.org/kits/youles-...
addgene.org
Addgene: Plant MoClo Tags, Promoters, Terminators, and Acceptors
A MoClo collection containing a selection of N-Terminal and C-Terminal tag modules, common plant promoters and terminators, and acceptor vectors.
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Plant Science Spotlight @plant-sci.bsky.social · 13/06/2026
Don’t reach for ChatGPT Images or Nano Banana as your first option when creating scientific images There are multiple free and open-source images, icons, and tools for creating scientific illustrations. #plantscience @stephenturner.us blog.stephenturner.us/p/free-open-...
blog.stephenturner.us
Free and open-source images, icons, and tools for creating scientific illustrations
Phylopic, NIH Bioart, Bioicons, Scidraw, Open Science Art, Health Icons, Servier Medical Art, Biodiversity Heritage Library, the Noun Project, Segment Anything, Excalidraw, draw.io, Biographics
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Michael Raissig @michaelraissig.bsky.social · 05/11/2025
1/ Preprint alert:
 🌾 The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution
 👉 doi.org/10.1101/2025... A 70k-cell single-cell RNA-seq atlas of the developing grass leaf—from the shoot meristem to mature leaf tissues. @cerealcell.bsky.social @lbmountain.bsky.social
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Dan Landau @landau.bsky.social · 04/06/2026
Exciting breakthrough technology from the lab, now live in @cellcellpress.bsky.social ! Instead of cutting the genome where proteins bind (e.g., Cut&Tag), D&D-seq scars the DNA with a deaminase, allowing single cell genome mapping of TFs and chromatin remodellers!
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James Lloyd 🧬 @jamespblloyd.bsky.social · 29/05/2026
New review out: The Promise of Synthetic Biology for Redesigning Plant Architecture Great to have worked on this with @suruchiroy.bsky.social and Gerad dos Santos! doi.org/10.3390/ijms...
doi.org
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Alejandro Montenegro @aemonten.bsky.social · 26/05/2026
"(...) plasmids are chromatinized in an organized, sequence-dependent manner and adopt a heterogeneous and incomplete chromatin architecture relative to nuclear-encoded chromatin fibers" www.cell.com/molecular-ce...
cell.com
Chromatin architectures underlying plasmid-based assays for regulatory variant effects
Mallory et al. develop plasmid Fiber-seq to map chromatin architecture along individual transfected plasmids at near-nucleotide resolution, which reveals that plasmids adopt heterogeneous chromatin pa...
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Eva-Sophie Wallner @eswallner.bsky.social · 05/12/2025
A light-induced apical auxin signalling minimum marks the site of organogenesis and de novo meristem formation in Marchantia sporelings (now peer-reviewed): authors.elsevier.com/a/1mDc83QW8S...
authors.elsevier.com
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Eva-Sophie Wallner @eswallner.bsky.social · 27/05/2026
For more information on my research, please visit: www.mpipz.mpg.de/wallner
mpipz.mpg.de
Polarity and morphogenesis of flat plant structures
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New Phytologist @newphyt.bsky.social · 14/05/2026
The secret behind stevia’s sweetness decoded by multi-omics science 📖 nph.onlinelibrary.wiley.com/doi/10.1111/... by Shoji et al. @WileyPlantSci #PlantScience
Biosynthetic pathway and genes involved in SG production in stevia.
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Chenxin Li, PhD @chenxinli2.bsky.social · 24/04/2026
Really interesting paper from @grey-monroe.bsky.social lab at UC Davis: www.pnas.org/doi/10.1073/... "Genome degradation in plant tissue culture" #PlantScience The data presented have a lot of implications both on basic biology and applications.
pnas.org
Genome degradation in plant tissue culture | PNAS
Many plants, unlike most animals, can reproduce as clones [D. Kester, HortScience 18, 831–837 (1983), 10.21273/hortsci.18.6.831]. Cloning plants is...
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Marcel Dickmanns | 🌿❄️🔬 @madic.bsky.social · 26/07/2025
New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM
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Molecular Plant & Plant Communications @mplantpcom.bsky.social · 25/12/2025
A Single-Cell-Resolution Spatial Transcriptomic Atlas Decodes Wheat Spike Development and Yield Potential #resource #MolecularPlant cell.com/molecular-pl...
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edgardemesa.bsky.social @edgardemesa.bsky.social · 08/01/2026
It's finally out!... Using single-cell and spatial transcriptome data, and data imputation, we created BARVISTA, a website able to perform almost genome-wide information on gene expression profiles at single-cell resolution in barley spikes! www.nature.com/articles/s41...
nature.com
Imputation integrates single-cell and spatial gene expression data to resolve transcriptional networks in barley shoot meristem development - Nature Plants
Spatially resolved gene expression during barley development was done by integrating an scRNA-seq dataset from cells with unknown position with spatial transcriptomics. This dataset is publicly availa...
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Rüdiger Simon @simonrdg.bsky.social · 26/05/2025
Meristems shape plant architectures, and grasses generate a complex array of them. To characterise barley vegetative SAMs and spike development, we used single cell and spatial transcriptome data and integrated them in a new database, BARVISTA. A click on a cell now..... tinyurl.com/38c3mf5d
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bioRxivpreprint @biorxivpreprint.bsky.social · 16/02/2026
Programmable and Switchable RNA Scaffolds for Synthetic Condensate Engineering in Mammalian Cells www.biorxiv.org/content/10.64898/20…
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Eftychis Frangedakis @e-frangedakis.bsky.social · 26/02/2026
The OpenPlant kit is finally available from Addgene as... a kit :) www.addgene.org/kits/haselof... @addgene.bsky.social #marchantia #synbio
addgene.org
Addgene: OpenPlant Kit
Use the OpenPlant toolkit for genetic engineering of the plant synthetic biology model Marchantia polymorpha.
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Clay Kosonocky @kosonocky.bsky.social · 04/03/2026
The results are finally in! 🏆💻🧬 I'm thrilled to announce that the manuscript for the Bits to Binders protein design competition is out on bioRxiv! Here's a summary of our findings, including some simple criteria that nearly *double* success rates when applied as a filter 🧵
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New Phytologist @newphyt.bsky.social · 11/03/2026
Advances in imaging Arabidopsis embryogenesis Babić et al. @istaresearch.bsky.social @milanzupunski.bsky.social @frimllab.bsky.social nph.onlinelibrary.wiley.com/doi/10.1111/...
Microscopical and computational visualisation of Arabidopsis thaliana embryos.
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Chenxin Li, PhD @chenxinli2.bsky.social · 24/02/2026
This paper is great. It describes a method to generate DNA binding proteins de novo: www.nature.com/articles/s41... I wish there is a web server where you can punch in DNA sequences, submit a job, then it generates the cDNA of your DNA binders, and you can go straight for synthesis & testing.
nature.com
Computational design of sequence-specific DNA-binding proteins - Nature Structural & Molecular Biology
The authors develop a computational method to design small DNA-binding proteins (DBPs) that target specific sequences. Designed DBPs show structural accuracy and function in both bacterial and mammali...
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Tominaga K. (tomiken) @pacyc184.bsky.social · 19/03/2026
Single-molecule peptide sequencing through reverse translation of peptides into DNA | Nature Biotechnology www.nature.com/articles/s41587-026-…
nature.com
Single-molecule peptide sequencing through reverse translation of peptides into DNA - Nature Biotechnology
Peptides are sequenced by converting each amino acid into amplifiable DNA barcodes.
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Molecular Plant & Plant Communications @mplantpcom.bsky.social · 13/03/2026
WIND1 controls cell fate transition through coordinately integrating histone acetylation and deacetylation-mediated transcriptional reprogramming during somatic embryogenesis #research #MolecularPlant cell.com/molecular-pl...
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Kai Johnsson @kjohnsson.bsky.social · 26/03/2026
A high-affinity split-HaloTag for live-cell protein labeling. And much more. www.nature.com/articles/s41...
nature.com
A high-affinity split-HaloTag for live-cell protein labeling
Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...
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bioRxiv Plant Bio @biorxiv-plants.bsky.social · 31/03/2026
DNA demethylation suppresses a state of enhanced cellular pluripotency and regeneration competence in Arabidopsis. www.biorxiv.org/content/10.64898/20…
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wverstraeten.bsky.social @wverstraeten.bsky.social · 01/04/2026
Recent highlights: Received seed funding from the SynGen Center to explore synthetic genomes in bottom-up synthetic biology. 🚀 The first work of my PhD is now published: onlinelibrary.wiley.com/doi/full/10.... Huge thanks to Franziska Gießler, Tobias Abele, Stefan Maurer, and @kgoepfrich.bsky.social
onlinelibrary.wiley.com
Growth, Dissolution and Segregation of Genetically Encoded RNA Droplets by Ribozyme Catalysis
Ribozymes enable sequence-encoded turnover of RNA droplet material, resulting in transiently active droplets that selectively segregate based on their genotype. This establishes a direct link between...
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François Parcy @flowerwhatelse.bsky.social · 10/02/2026
Have you ever wondered why some petal are bicolor ? Like in dahlias or petunia ? (Part of) the answer is in this publication : doi.org/10.1093/pcp/...
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Dominik Handler @86dominik.bsky.social · 13/02/2026
How does the piRNA pathway solve the self vs. non-self problem? 🧬 Since piRNAs come from single-stranded RNA, how does the cell choose the right ones? For years, "piRNA clusters" were seen as THE privileged source. But are they really special and earmarked for biogenesis? (1/19)
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