By Dr. Martin Kessler, Postdoctoral Fellow, Department of Cell Biology, University of Pennsylvania

I never wrote up the other repo I actually use the most, which is Hyde, the thing this blog runs on. Btw, now is the time to start YOUR blog, really.

This Article introduces a phospho-switch for allosterically regulating target proteins in response to ERK kinase activity.

Translational regulation is a critical component of the cellular response to environmental stress. Ionizing radiation (IR) exists naturally at low doses (e.g., cosmic rays and radioactive materials) b...

To date, collision-induced dissociation and methods based on electron transfer dissociation are considered the ‘best’ approaches for the mass spectrometry analysis of N- and O-glycopeptides, respectiv...

Analytical ChemistryDOI: 10.1021/acs.analchem.6c02479

Mass Spectrometry Reviews, EarlyView.

Despite extensive research, molecular differences in human populations and the influence of ancestry, age, geography, and diet are poorly understood. …

Umbrella sampling is a powerful tool to investigate the underlying free-energy landscapes that govern the permeation properties of small molecules through complex lipid bilayers. However, obtaining co...

Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00389

O-glycan analysis faces analytical challenges due to poor fragmentation characteristics of oxidatively released O-glycan acids, which can preserve labile O-acetyl modifications but suffer from charge ...

Nature Protocols, Published online: 22 April 2026; doi:10.1038/s41596-026-01354-wThe peptide-centric local stability assay (PELSA) is used to identify ligand targets and binding regions at the proteome scale. By subjecting native proteins to brief yet extensive trypsin digestion, this method generates local stability profiles.

Sensory neuron subtypes are defined by transcriptomics, but their proteomic identities remain unclear. Here, authors show distinct protein signatures of electrophysiologically defined nociceptors and ...

Does HIV-1 package host proteins that promote infection? Using in virion RNA interactome capture, Garcia-Moreno et al. mapped human proteins bound to the viral genome inside virions. They identified o...