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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 07/07/2026

🚨New preprint!🚨 Lack of specific affinity reagents hinders studies of signalling mechanisms. We show that sdAbs provide a robust scaffold for making linkage-specific ubiquitin binders, exemplified by 2A6, a versatile reagent for M1-linked ubiquitin chains. www.biorxiv.org/content/10.6...

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Rune Busk Damgaard @rbdamgaard.bsky.social · 07/07/2026

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 26/03/2026

Cell Stress & Inflammation - CSI - is my group's new name. We are still incredibly interested in ubiquitin, but our new name reflects a broadening scope in the lab, in part based on our recent paper (www.nature.com/articles/s41...). Visit our new website here: www.bioengineering.dtu.dk/csi

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Rune Busk Damgaard @rbdamgaard.bsky.social · 26/03/2026

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Fletcher Lab @fletcherlab.bsky.social · 24/03/2026

Receptor clustering is a common theme in viral detection, but whether detection directly hinders genome replication is less clear. Excited to share the Z in RZ-type E3s: ZNFX1 is an RNA helicase that builds ubiquitin chains leading to aggregates that ensnare viral RNA. www.cell.com/molecular-ce...

cell.com

ZNFX1 uses two-component ubiquitin circuitry to quarantine viral RNA

Squair et al. show that ZNFX1 restricts ssRNA virus replication by integrating SF1 helicase activity with dual E3 ligase mechanisms. ATP-dependent RNA binding activates E3 function, driving self-propa...

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/03/2026

I’d take a full review from a grumpy Reviewer #2 any day over student teaching evaluations. Students are savage.

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 07/03/2026

More evidence that LUBAC has functions beyond inflammation: Our study shows that LUBAC regulates AMPK signalling to control metabolic adaptation, autophagy, and cell death. This might explain the metabolic problems observed in LUBAC deficiency and ORAS patients. www.nature.com/articles/s41...

nature.com

M1-linked ubiquitination by LUBAC regulates AMPK signalling and the response to energetic stress - Cell Death & Differentiation

Cell Death & Differentiation - M1-linked ubiquitination by LUBAC regulates AMPK signalling and the response to energetic stress

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Rune Busk Damgaard @rbdamgaard.bsky.social · 07/03/2026

nature.com

M1-linked ubiquitination by LUBAC regulates AMPK signalling and the response to energetic stress - Cell Death & Differentiation

Cell Death & Differentiation - M1-linked ubiquitination by LUBAC regulates AMPK signalling and the response to energetic stress

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 24/02/2026

This project started with confusion 🤔 www.nature.com/articles/s41... Loss of LUBAC or OTULIN causes severe inflammatory disease. But patients also show clear metabolic defects. Neither LUBAC nor OTULIN had been linked to metabolic regulation. So where do these metabolic manifestations come from?

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Rune Busk Damgaard @rbdamgaard.bsky.social · 24/02/2026

We turned to proteomics 📊 and found that LUBAC and OTULIN regulate AMPK. This suggests the metabolic defects may not simply be secondary to inflammation. Rather, they may be central in to the pathogenesis.

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Rune Busk Damgaard @rbdamgaard.bsky.social · 24/02/2026

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 16/02/2026

🧐 What if metabolic defects are not just a consequence of inflammatory disease - but actively contribute to it? Inflammation and metabolism are deeply intertwined, but the molecular links remain unclear. Our new paper in Cell Death & Differentiation uncovers one. 🧵👇 nature.com/articles/s41...

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/02/2026

Huge congratulations to Camilla Elbæk, co-authors, and amazing collaborators on this effort 👏 @keisakamoto.bsky.social @labsakamoto.bsky.social, @annikameinander.bsky.social, Grahame Hardie...

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/02/2026

💡 This links inflammatory ubiquitin signalling directly to metabolic control. Could metabolic dysfunction be a driver - not just a consequence - of inflammatory disease?

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/02/2026

🔬 Using cell lines 🧫, mice 🐁, flies 🪰, and patient cells 🏥, we found: ✔️ LUBAC + OTULIN regulate AMPK during energetic stress ✔️ M1-linked ubiquitination promotes AMPK activity and metabolic adaptation ✔️ Loss of this axis reduces cell and organism resilience to starvation

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/02/2026

👉 We show that M1-linked ubiquitination by LUBAC directly regulates AMPK, the master energy sensor of the cell. LUBAC and M1-linked ubiquitin are best known for regulating inflammatory signalling and cell death 💀☠️. But they also control metabolic signalling and adaptation.

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/02/2026

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Reposted by Rune Busk Damgaard

UK Cilia and Centrosome Network @ukcilianetwork.bsky.social · 11/02/2026

Interesting article and accompanying spotlight on the cross-talk between ciliary homeostasis and ubiquitination (written by Jonathan Pruneda and @rbdamgaard.bsky.social). www.nature.com/articles/s41... www.nature.com/articles/s41...

nature.com

A tale of two tags: UFMylation counters ubiquitination for ciliary homeostasis - Cell Death & Differentiation

Cell Death & Differentiation - A tale of two tags: UFMylation counters ubiquitination for ciliary homeostasis

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 29/01/2026

💡 Ciliary homeostasis turns out to depend on an unexpected tug-of-war between UFMylation and ubiquitination. In a recent editorial in Cell Death & Differentiation, @jnpruneda.bsky.social and I highlight new work from Wang et al. 🧵👇

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Rune Busk Damgaard @rbdamgaard.bsky.social · 29/01/2026

This work expands the landscape of PTM crosstalk and raises the possibility that ciliary dysfunction contributes to neurological and developmental disorders linked to the UFM1 pathway. 🔗 www.nature.com/articles/s41...

nature.com

A tale of two tags: UFMylation counters ubiquitination for ciliary homeostasis - Cell Death & Differentiation

Cell Death & Differentiation - A tale of two tags: UFMylation counters ubiquitination for ciliary homeostasis

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Rune Busk Damgaard @rbdamgaard.bsky.social · 29/01/2026

Mechanistically, UFMylation and ubiquitination compete for the same lysine on IFT88, forming a mutually exclusive modification switch that controls protein stability and ciliary integrity across tissues.

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Rune Busk Damgaard @rbdamgaard.bsky.social · 29/01/2026

While ubiquitination is a well-known regulator of protein stability, the roles of many ubiquitin-like modifiers remain unclear. Here, UFL1-mediated UFMylation emerges as a protective signal for the ciliary protein IFT88.

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Rune Busk Damgaard @rbdamgaard.bsky.social · 29/01/2026

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 21/11/2025

🚨 Postdoc position in my lab! A project on protein binder discovery for K6-linked #ubiquitin to investigate their role in infection is available. 📍 DTU, Copenhagen, Denmark ⏰ Deadline: 18 December 2025 Apply here: efzu.fa.em2.oraclecloud.com/hcmUI/Candid...

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Rune Busk Damgaard @rbdamgaard.bsky.social · 21/11/2025

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Rune Busk Damgaard @rbdamgaard.bsky.social · 30/10/2025

A #postdoc position in atypical #ubiquitin signalling and protein binder engineering is opening soon in my lab at DTU Bioengineering! Get in touch if you are interested.

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Rune Busk Damgaard @rbdamgaard.bsky.social · 07/10/2025

🚀 Off to our Research Retreat with the Section for Medical Biotechnology, DTU Biotechnology and Biomedicine. Two days of science, ideas & innovation 🧬💡 12 faculty pitches, 30+ PhDs & postdocs, and endless opportunities for new collaborations 🔬🤝✨

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 16/09/2025

A few months ago, our review “The Molecular Toolbox for Linkage Type-Specific Analysis of Ubiquitin Signaling” 🧬🧰🛠️ came out in ChemBioChem. Time to give it a spotlight! chemistry-europe.onlinelibrary.wiley.com/doi/full/10....

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/09/2025

Hope this serves as a useful overview for anyone working on ubiquitin signalling or PTMs. Big thanks to those who did the heavy lifting in the writing process: Julian Koch, Camilla Reiter Elbæk & @dompri.bsky.social 🙌😊

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/09/2025

We also highlight: ✅ Trade-offs between specificity, throughput, & sensitivity ✅ Gaps in the current toolbox where innovation is needed ✅ The value of combining orthogonal methods for robust insight

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/09/2025

…alongside analytical platforms such as: ✅ Immunoblotting 🧫📊 ✅ Fluorescence microscopy 🔬🌈 ✅ Enzymatic assays 🧪⏱️ ✅ Mass spectrometry ⚡📈

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/09/2025

In the review, we summarise state-of-the-art tools for probing linkage-type specificity: 🎯 Antibodies & antibody-like molecules 🎯 Affimers 🎯 Engineered Ub-binding domains 🎯 Catalytically inactive deubiquitinases 🎯 Macrocyclic peptides

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/09/2025

Why this matters ⬇️ Ubiquitin chain diversity, i.e. different linkage types, mixed or branched chains, is central to cell signalling. But analysing these chain types specifically remains a big challenge.

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Rune Busk Damgaard @rbdamgaard.bsky.social · 16/09/2025

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Rune Busk Damgaard @rbdamgaard.bsky.social · 14/08/2025

Is it still useful to insist that authors spell out every protein name in research papers? Many names are long, obscure, and say nothing about function... we might as well call them James, Joan, and John.

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Reposted by Rune Busk Damgaard

TanentzapfLab @tanentzapflab.bsky.social · 13/08/2025

The goal of a PhD is not to learn some facts or read a few papers or learn a bunch of techniques. The goal of a PhD is to learn independence, problem solving, how to finish things you start, resilience, & gain the ability to adapt & think creatively. Learning these things is hard.

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Reposted by Rune Busk Damgaard

Richard Sever @richardsever.bsky.social · 02/07/2025

the really key number to remember is that biorxiv serves the entire global science community, posting thousands of papers per month for millions of readers, and it's entire running costs ( a few $M/year) are less than some single labs....

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Reposted by Rune Busk Damgaard

Prasad Jallepalli, MD, PhD @prasad.bsky.social · 23/05/2025

it’s a global contest, whether we want to compete or watch from the sidelines

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Reposted by Rune Busk Damgaard

Sakamoto Lab @labsakamoto.bsky.social · 16/05/2025

Special day for our group @cbmr.science as we celebrate the successful defense of our (first) freshly minted PhD, Dr. Conchita Bringas 🥳 Her thesis was based on characterizing novel #AMPK inhibitors. Special thanks to the great committee @rbdamgaard.bsky.social ,Dave Carling & Lykke Sylow.

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Reposted by Rune Busk Damgaard

David Vilchez @thevilchezlab.bsky.social · 15/05/2025

We have a 4-year postdoc position open in our Research Unit on proteostasis! The project explores inter-tissue regulation using advanced proteomics approaches. Collaborative & interdisciplinary environment. More details & application here: jobs-uk-koeln.onlyfy.jobs/job/pktg6ivj...

jobs-uk-koeln.onlyfy.jobs

Postdoc Position (f/m/x)

We are looking to support our rapidly growing team as soon as possible:

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Rune Busk Damgaard @rbdamgaard.bsky.social · 13/05/2025

Haven’t shared this in a while. But the #ubiquitin and #ubl Starter Pack now includes 116 profiles to follow if you’re interested in the field: signalling, structure and biochemistry, biology, mechanisms, proteostasis, TPD, chemical biology… Check out the list and let me know if you are missing.

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Reposted by Rune Busk Damgaard

Rune Busk Damgaard @rbdamgaard.bsky.social · 22/04/2025

Happy to see our review out in ChemBioChem @chemistryeurope.bsky.social in which we describe the tools and strategies for deciphering linkage type-specific #ubiquitin signalling: doi.org/10.1002/cbic.... Thanks to Julian for spearheading this, and to Camilla and Dominik for important contributions.

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