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Big thanks to our entire dream-team of imaging and proteomics experts, especially collaborators @rhodamine110.bsky.social and @dyerfulchymist.bsky.social

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Some benefits of this approach include: no overexpression, imaging to confirm localization in parallel with proteomics, and general compatibility across baits

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Inspired by our POCA-1.0 platform www.nature.com/articles/s41... and the OMAP platforms elifesciences.org/reviewed-pre... pmc.ncbi.nlm.nih.gov/articles/PMC... here we we append photosensitizers, including JF570 and DBF to oligo and antibodies for in situ proximity labeling of fixed cells

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Keri Backus @keribackus.bsky.social · 29/04/2026

Super excited to launch POCAxOMAP with @dschweppe.bsky.social and @oligopain.bsky.social and led by Eli Biletch, Conor Herlihy and Lidan Li. If you’ve ever wanted to do proximity labeling on DNA, RNA, or protein without genetic engineering, look no further. www.biorxiv.org/content/10.6...

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Congrats to all award recipients and special recognition to our lab’s own Caroline Canavan!

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And likely my all-time favorite experiment in this manuscript was our head-to-head comparison of asciminib and dasatinib probes, which perfectly resolved the known binding sites on ABL1 kinase and provides a nice confirmation of both the method and PAL more generally

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Like @sinzandrea.bsky.social has reported pubs.acs.org/doi/10.1021/... for crosslinking MS, we found that the photocrosslinked modifications likely fragment to release the modification, which can result in MS/MS spectra without shifted ions

pubs.acs.org

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Consistent with prior studies, we saw a substantial amount of diazirine labeling of acidic residues, which results in a quite labile ester bond

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Lots of stuff I'm really excited about in this paper, including the new localization hyperscores in #FragPipe to guide pinpointing of labeled residues., including for peptides without any shifted ions at all

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Big congrats to first authors Chau Ngo and Sho Takechi and a big thank you to all the authors and especially to our collaborators including @nesvilab.bsky.social @fcyucn.bsky.social @marcliesa.bsky.social and Ajit Divakaruni

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Devin Schweppe @dschweppe.bsky.social · 07/04/2026

Super excited that our SEE-CITE photoaffinity labeling method is now out in @natchem.nature.com rdcu.be/ffBSC. Here we introduce a custom silyl ether cleavable linker between a diazirine photocrosslinker handle and molecules of interest to streamline site-of-labeling analysis for PAL chemoproteomics

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Reposted by Keri Backus

The next iteration of O-MAP targeting genomic loci is now at eLife!! DNA O-MAP enables proximity labeling at specific DNA loci took a ton of work from many lab members and collaborators, so it's really exciting to wrap up this paper with @oligopain.bsky.social! elifesciences.org/articles/102...

elifesciences.org

DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci

DNA O-MAP enables proximity labeling at specific genomic loci in fixed cells using programmable oligonucleotides, revealing locus-proximal proteomes and chromatin interactions without genetic modifica...

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And lastly, Andrew is on the job hunt for Bay area-adjacent positions. So, if anyone is looking to hire a stellar organic chemist turned chemical and systems biologist, look no further

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And thanks to the reviewers and editors--this one was a journey and the final product really is light years from our preprint.

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Also big shoutout to @rhodamine110.bsky.social for his amazing generosity in sharing dyes. JF570 is a very special molecule!

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So many wonderful collaborators helped make this project happen, including Zander Nguyen, JP Kennelly, Julia Mack, @soongookhong.bsky.social, Rohith Nagari, LJ Cui, Xu Xiao, and especially Peter Tontonoz, who introduced me to the wild world of lipids.

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