This Review considers developments in enzymes, biosynthetic pathways and cellular engineering that enable their use in catalysis for new chemistry and beyond.

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

Background Terpene synthases (TPSs) are enzymes that catalyze some of the most complex reactions in nature—the cyclizations of terpenes, which form the carbon backbones to the largest group of natural...

Abstract. Antimicrobial resistance (AMR) is a global health threat, yet, despite this, antibiotic drug discovery has stagnated. Most compounds entering the

Abstract. Site-specifically attaching biorthogonal handles to proteins is an essential tool for chemical biology research and diverse applications, includi

Tan and colleagues develop DNA nanodevices to detect the pH of the lysosomal outer surface, observing an acidic layer generated by TMEM175 that regulates lysosome positioning in response to changes in...

An iron-based, enzyme-triggered CO-releasing molecule (ET-CORM) is site-specifically conjugated to the antibody trastuzumab. This novel ET-CORM-antibody conjugate releases carbon monoxide upon cleava...

A chemoproteomic approach is developed that examines changes in ligand binding-induced accessibility by globally labeling reactive proteinaceous lysines, revealing the cellular targets of glycolytic i...

A photoswitchable glucocorticoid receptor agonist enables optical control of receptor activity, achieving picomolar potency and partial agonism that preferentially maintains anti-inflammatory signali...

Massive library-based platforms enable rapid early hit discovery, yet subsequent hit resynthesis and validation remain major bottlenecks in drug discovery. This delay is amplified when optimizing hits...

“My favorite molecule is alpha-ketoglutarate because it is structurally simple yet performs an astonishing variety of functions in the cell… The most exciting thing about my research is bridging scal...

Bioisosteric replacement is a powerful strategy for optimizing physicochemical, pharmacological, and safety properties of drug candidates. In this article, we provide a bioisosteres cheat sheet comple...