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Guillaume Gaullier

@guillawme.eurosky.social
751 followers 210 following 119 posts

Structural biologist using #cryoEM ❄️🔬 I feel more at home on the Fediverse at fediscience.org/@Guillawme

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Guillaume Gaullier @guillawme.eurosky.social · 19h
Ref for the "antibody-gate" scandal mentioned in the previous post: reeserichardson.blog/2026/08/25/a...
reeserichardson.blog
At least 15 companies are selling antibodies using faked validation data
Popular vendors’ catalogs feature more than 18,000 manipulated images
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Guillaume Gaullier @guillawme.eurosky.social · 19h
Are these honest western blots done on unintentionally wrong cell lines? Faked western blots done with malicious intent? Honest western blots done on correct cell lines but with unintentionally wrong antibodies from one of the 15 companies selling antibodies with faked validation data? 😵‍💫
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Structural Biology @actacrystd.iucr.org · 20/10/2026
A method for rapidly depositing, thinning and vitrifying samples for cryoEM on the sub-millisecond timescale #CryoEM #TimeResolvedCryoEM #StructuralBiology doi.org/10.1107/S205...
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cryoEM papers @cryoempapers.bsky.social · 17/10/2026
Variable-resolution maps (VRMs) in CCTBX and Phenix: accounting for local resolution in cryoEM pubmed.ncbi.nlm.nih.gov/42878395/ #cryoEM
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Guillaume Gaullier @guillawme.eurosky.social · 19/10/2026
The model building was very challenging with all these co-factors, I would not have managed without ISOLDE so thanks a lot @crolltristan.bsky.social !!
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Guillaume Gaullier @guillawme.eurosky.social · 19/10/2026
Very happy to see this paper published! Got an excellent batch of monomeric photosystem I prepared by 1st author Jelena Boyka: the microscopy and image processing were a breeze (also thanks to the excellent #cryoEM facilities at @uu.se and @scilifelab.se). doi.org/10.1007/s111...
doi.org
High-resolution structure of monomeric photosystem I from Thermosynechococcus vestitus - Photosynthesis Research
Photosystem I (PSI) exists in trimeric (tPSI) and monomeric (mPSI) forms within the thylakoid membrane of cyanobacteria. PSI functions as a light-driven electron pump, enabling the transfer of electro...
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Guillaume Gaullier @guillawme.eurosky.social · 16/10/2026
Were any attempts made at crystallizing bears?? 😆
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Guillaume Gaullier @guillawme.eurosky.social · 15/10/2026
This has always looked like chromatin to me. Some seats in other public transports in France have patterns that look to me like ribosomes, but I don't have a picture.
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pilarcossio.bsky.social @pilarcossio.bsky.social · 14/10/2026
1/5 Cryo-EM gives high-resolution RNA structures, but only when high Mg²⁺ freezes out their motion. At physiological conditions, RNAs are dynamic and 3D reconstruction fails. Can we still learn their folding equilibria? New preprint: yes, straight from the raw particle images👇
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RCSB Protein Data Bank @rcsbpdb.bsky.social · 13/10/2026
As the PDB turns 55 next week, take a look at the dozen historic structures that started it all
pdb101.rcsb.org
PDB101: Molecule of the Month: PDB Pioneers
A dozen historic structures set the foundation for the PDB archive
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Przemek Dutka @przemekdut.bsky.social · 08/10/2026
Our latest development is now on bioRxiv: cryoSHIELDs — amphipathic peptide additives that make cryo-EM grid prep far more reliable. Tested across 24 protein systems, they enabled the first ABCC6 structure and GPCR–G protein structures at just 0.1–0.5 mg/mL! www.biorxiv.org/content/10.6...
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cryoEM papers @cryoempapers.bsky.social · 06/10/2026
Structural basis of PARP2 recognition of nucleosomal DNA breaks and inhibitor trapping pubmed.ncbi.nlm.nih.gov/42831429/ #cryoEM
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Prof William Ripple @williamripple.bsky.social · 28/09/2026
We invite everyone (nonscientists) to endorse the Declaration of a Planetary Emergency, part of the upcoming World Scientists’ Warning to Humanity: A Third Notice. Add your name and share widely. Endorsement helps show public concern about the future of our planet. forms.gle/vAARd7mv2yS7...
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Basil Greber @bjgreber.bsky.social · 01/10/2026
Those who had a careful look at the methods section of our work on the #cryoEM structure of the CDK11-cyclin L-SAP30BP complex will have noticed that we added a short peptide to our sample before freezing. If you wonder why...
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Structural Biology @actacrystd.iucr.org · 28/09/2026
After analyzing 72 structures of NcAA9D, it was found that X-rays progressively reduce the active-site copper from Cu(II) to Cu(I), forming an electron-density feature that is easy to misinterpret #RadiationDamage #LPMOs #DioxygenBinding doi.org/10.1107/S205...
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Yuan He @yuanhejhu.bsky.social · 22/09/2026
A big week for the lab! Two new papers on how cells repair DNA double-strand breaks: one on getting NHEJ started in chromatin, one on processing the ends before ligation. Years of work by a fantastic team.
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Guillaume Gaullier @guillawme.eurosky.social · 18/09/2026
Interesting, but the code repository says "Anthropic does not plan further updates and does not accept pull requests". So they seem more interested in the PR stunt than in supporting science on the long term...
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bioRxiv Biophysics @biorxiv-biophys.bsky.social · 17/09/2026
The CAHRA Challenge: A Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms www.biorxiv.org/content/10.64898/20…
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cryoEM papers @cryoempapers.bsky.social · 18/09/2026
Strategies for time-resolved cryogenic electrontomography of transient cellular processes pubmed.ncbi.nlm.nih.gov/42748555/ #cryoEM
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Sergey Ovchinnikov @sokrypton.org · 17/09/2026
Introducing highly experimental localfold.org Building on @martinsteinegger.bsky.social af2 webgpu port, @milot.bsky.social optimizations & jax ports of af3-like models by @marielle.bsky.social, Choonghwan Lee, Julia Buhmann. WARNING: runs directly on your 💻, may drain 🪫 & eat data📱 & overheat 🔥💻
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Basil Greber @bjgreber.bsky.social · 16/09/2026
Is there a recent review that summarises all the biggest, baddest, most mind-boggling #cryoEM structures out there? What I need is basically just 10 pages worth of "look at this crazy thing!" Suggestions welcome! 😆
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Marcin J. Suskiewicz @msuskiewicz.eurosky.social · 15/09/2026
Today, the day of the 100th anniversary symposium for Jean-Pierre Serre in Paris, I'm thinking of this quote from another mathematician, A. Connes. I do think it's important we foster confidence in our own expertise - as a nonsense filter and departure point for creativity.
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Joy Jeongyoon Choi @joyjoychoi.bsky.social · 14/09/2026
Excited to share our new preprint on in situ chromatin structure of the inactive X chromosome in differentiated female mouse embryonic stem cells. www.biorxiv.org/content/10.6... 🧵1/8
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Harmit Singh Malik @harmitmalik.bsky.social · 13/09/2026
But if you gain fulfillment from the doing & the teaching, from the achievements of others, including your competitors, from those that came before you, & those that will follow, you are somewhat insulated. Ultimately, this is a good moment to reflect on what brings YOU joy & act accordingly.
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Structura Biotechnology @structurabio.bsky.social · 10/09/2026
💡 The idea is simple: getting to know your data is often the shortcut to more efficient particle picking. In our latest #CryoSPARC blog post, we cover how to speed up #cryoEM data processing through efficient early curation of your dataset. 👉 cryosparc.com/blog/curatio...
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NBIS @nbis.se · 08/09/2026
A senior bioinformatician working on your project in Sweden for up to 500 hours! Apply to the next call for Bioinformatics Support Peer review track (aka WABI): www.nbis.se/services/bio... Deadline: October 1st 2026 (23:59)
nbis.se
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Juan Carlos De la Concepcion @delaconcepcionjc.bsky.social · 08/09/2026
The beauty of posting in @biorxivpreprint.bsky.social. This amazing tool was available for the community long before it showed up on a journal. I even forgot this publication was not "peer-reviewed", but it was definitively "community-reviewed". Kudos to @lorenzlamm.bsky.social and all the authors!
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Guillaume Gaullier @guillawme.eurosky.social · 07/09/2026
"How contemporary academic structures constrain scientific creativity and hold back early-career researchers" doi.org/10.1038/s415... #AcademicChatter #Academia
doi.org
How contemporary academic structures constrain scientific creativity and hold back early-career researchers - Nature Human Behaviour
In this Perspective, Haubrock et al. argue that today’s scientific structures constrain creativity and that strategic conformity is a rational response. They discuss how this holds back early-career r...
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Magnus Bauer @kinasekid.bsky.social · 06/09/2026
AlphaFold Server’s ligand menu just got a lot bigger and I almost missed it! You can now add any ligand from the wwPDB Chemical Component Dictionary. Just enter its CCD code 🥳
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Guillaume Gaullier @guillawme.eurosky.social · 03/09/2026
Thanks Cody! Hope all is well with you. 🙂
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Guillaume Gaullier @guillawme.eurosky.social · 02/09/2026
This paper was a nice side quest that started as a random encounter with @hrbridges.bsky.social at the poster session of the 2025 @ccpem.bsky.social Spring Symposium. It was very satisfying to finally see the macro domain of ALC1 that had escaped all previous structures of ALC1-nucleosome complexes!
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Florent Waltz @florentwaltz.bsky.social · 07/08/2026
We've been seeing a lot of "in situ" lately in titles from fancy journals (not pointing any fingers 👀). I’ve delayed posting this, but as more and more come out, I'm afraid "in situ" is becoming an empty buzzword, much like "near-atomic" was for SPA 🔬 1/n #cryo-ET #cryo-EM #cryoET #cryoEM 🧪
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Martin Steinegger 🇺🇦 @martinsteinegger.bsky.social · 30/08/2026
Run AlphaFold2 locally in your browser via WebGPU, no installation needed, it's just a static webpage using your own GPU or CPU. Short proteins run in seconds. Larger ones are still slow: a 291-aa protein takes ~7 minutes on my M4 Pro. ❗It's just a PoC. 🌐 martin-steinegger.github.io/alphafold2-w...
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Structural Biology Communications @actacrystf.iucr.org · 27/08/2026
WebCalEM is a browser-based tool that makes lattice-based pixel-size calibration a routine step in the cryo-EM workflow #CryoElectronMicroscopy #MagnificationCalibration #PixelSize doi.org/10.1107/S205...
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Reese Richardson @reeserichardson.bsky.social · 25/08/2026
A massive update: At least ~15 companies~ are selling scientists antibodies using faked validation data. We've documented 18,000+ manipulated images on 17,000+ products sold by leading laboratory suppliers including Thermo Fisher, Abcam, Santa Cruz Biotechnology, Millipore Sigma and Bio-Techne. 1/🧵
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Nature Methods @natmethods.nature.com · 24/08/2026
A Kaggle challenge for particle picking in cryo-ET data spurred the development of new algorithms that outperform state of the art software. www.nature.com/articles/s41...
nature.com
Lessons learned from a Kaggle challenge for particle picking in cryo-electron tomography - Nature Methods
A Kaggle challenge for particle picking in experimental cryo-electron tomography data delivered new machine learning algorithms that outperformed state-of-the-art software and provides a critical refe...
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Structural Biology @actacrystd.iucr.org · 24/08/2026
AlignPCA-2D is a principal component analysis-space Euclidean vector alignment method for fast, interpretable 2D classification in cryo-EM #3DReconstructionAndImageProcessing #SingleParticleCryoEM doi.org/10.1107/S205...
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Protein Data Bank in Europe (PDBe) @pdbeurope.bsky.social · 06/08/2026
Out now in Journal of Applied Crystallography: mmCIF Validator, automated validation of mmCIF files against the PDBx/mmCIF dictionary Useful for structure deposition prep, biocuration, and automated QC (VS Code extension + Python/CLI) Paper: doi.org/10.1107/S160... Code: github.com/PDBeurope/mm...
doi.org
mmCIF Validator: a comprehensive validation tool for structural biology data files
The mmCIF Validator provides real-time automated validation of mmCIFs against a valid PDBx/mmCIF dictionary. The validator is available as both a Visual Studio Code extension and a standalone Python s...
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Structural Biology Communications @actacrystf.iucr.org · 30/07/2026
A new type of article for Acta F: Protocols The purpose of this new category is to provide to the reader procedures that are sufficiently detailed so other researchers can reproduce the described workflow. Find out more in 'Show me how it's done: introducing Protocols': doi.org/10.1107/S205...
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Prism Science @prismscienceorg.bsky.social · 29/07/2026
An mmCIF file can say a residue has two alternate conformations and how often each appears. It cannot say which alternates occur together in the same copy. A draft proposal for recording that correlation, plus a tool to inspect it: diffuse.science/posts/mmcif-...
diffuse.science
mmCIF Explorer and the Heterogeneity Proposal
A playground for the PDBx/mmCIF file exploration, a look at the current proposal for encoding correlated heterogeneity
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Guillaume Gaullier @guillawme.eurosky.social · 28/07/2026
I experienced something similar last year (new thing found in an old dataset, as a minor species). Really cool to see previous datasets revisited! We should all do it when there is any suspicion that something might have been missed.
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Carl T. Bergstrom @carlbergstrom.com · 27/07/2026
A question: does anyone know about communities or groups for scientists who are committed to writing without the use of LLMs / genAI?
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Bui Lab @McGill @builab.bsky.social · 20/07/2026
Interested in 3D-printed models of cryo-EM grids, autogrid and cryo-FIB for visual teaching/decoration. Download our model and print: makerworld.com/en/models/30... Designed in Blender by Khan Bao, an undergrad in the lab.
makerworld.com
Cryo-EM Grids for Teaching - Free 3D Print Model - MakerWorld
Download this free 3D print file designed by CiliaBuilder. EM Grid Scaled ModelScaled up and simplified 3D model of EM grids and autogrids for demonstration of preparation process and milling. Content...
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Structura Biotechnology @structurabio.bsky.social · 21/07/2026
🧩 Why can two refinements with very different particle counts produce nearly identical maps? Our new “Digging Deeper” series breaks down #CryoSPARC case studies in a practical way, starting with how refinement parameters shape reconstruction quality. Read more 👉 cryosparc.com/blog/paramet...
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Michael Hothorn @structplantbio.bsky.social · 26/06/2026
7. For scientific progress and OUR understanding of the world this is of course very bad news. The only way to change this is to refocus on discovery NOT being prolific. In our days Galileo would have had not time to look at Jupiter, he would have reported telescopes in different colors instead.
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Richard Sever @richardsever.bsky.social · 25/06/2026
This is an interesting idea. Since journals are keen to make authors declare when they have used AI, might authors want editors to do the same...?
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Guillaume Gaullier @guillawme.eurosky.social · 26/06/2026
If you posted your preprint under a CC-BY license, you already gave permission to this kind of re-use, whether you like it or not. But very relevant question for preprints posted with the option "all rights reserved", or under a CC license with clauses NC (non-commercial) and/or ND (no derivatives).
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Dr. B. Duygu Özpolat (she/her) @biyolokum.bsky.social · 25/06/2026
It is just so unfortunate that you chose to fuel a similar kind of "top dog / alpha male" culture by using language (1%) that is associated with extremely rich people whose ethics are questionable at best. I am actually excited about what you are doing with QED, this "best" papers thing...
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Guillaume Gaullier @guillawme.eurosky.social · 25/06/2026
"At least doing something", but will automated review fix the broken incentive of maximizing number of papers for bean-counting evaluation? It might in fact encourage an even higher paper throughput, i.e. the opposite of what we need if we want to keep up and still have time to think...
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Guillaume Gaullier @guillawme.eurosky.social · 25/06/2026
He said he wanted to do away with journals, not paywalls... 😜 (I find the mandatory sign-up annoying, I already have enough accounts. If it won't be fully public, at least offer ORCiD login so the alternatives aren't only to create yet another account or go through Google.)
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