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Reposted by Filipe Carvalho

Biology OA @biologyoa.bsky.social · 01/06/2025

This paper advances deep proteome sequencing, improving detection of protein variants & isoforms beyond standard shotgun proteomics. By analyzing six human cell lines, it identifies a million unique peptides from 17,717 protein groups, highlighting translation of most alternatively spliced isoforms.

doi.org

Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology

Deep proteome sequencing achieves ~80% coverage of the human proteome.

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