Reposted by Biology OA
This paper advances deep proteome sequencing, improving detection of protein variants & isoforms beyond standard shotgun proteomics. By analyzing six human cell lines, it identifies a million unique peptides from 17,717 protein groups, highlighting translation of most alternatively spliced isoforms.
doi.org
Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology
Deep proteome sequencing achieves ~80% coverage of the human proteome.