Daniel McManus, PhD @danmcmanus123.bsky.social · 03/10/2026inpreparation.substack.com/p/opinion-re...inpreparation.substack.comOpinion: I Miss When The NIH Payline Was 40% And We All Mouth-Pipetted PhenolBy Dr. Douglas R. Waverly, Professor Emeritus of Biochemistry, Yale University 000
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 01/10/2026Upcoming OBS Immunology Seminar 'TIF1γ regulates stability of regulatory T cells during inflammation' (Nat. Immunol. 2026) Eugenio Contreras-Castillo, PhD Postdoc, Davis Lab Stanford Moderator: Maria A. Cardenas, PhD Postdoc, Wherry Lab, UPENN Thurs, Oct 8th, 2026 5pm ET | 4pm CT | 2pm PT Zoom: 153
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 01/10/2026Upcoming OBS Immunology Seminar Moderator highlight! Maria A. Cardenas, PhD Postdoc, Wherry Lab, UPENN Thurs, Oct 8th, 2026 5pm ET | 4pm CT | 2pm PT Zoom: 131
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 18/09/2026Upcoming OBS Immunology Seminar 'Intratumoral T cell activation kills tumors regardless of T cell specificity' (Nat. Immunol. 2026) Olivia C. Ghirardelli Smith, PhD MD/PhD student, Masopust Lab University of Minnesota Thurs, Sept 24th, 2026 3pm ET | 2pm CT | 12pm PT Zoom 👇️ 175
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 07/06/2026OBS Immunology Seminar| Young Investigator Highlight 'Decoding T Cell Exhaustion: An Epigenetic Roadmap to Enhance Cancer Immunotherapy' Hazem E. Ghoneim, PhD Assoc. Prof. The Ohio State University Thurs, June 11th, 2026 11am ET | 10am CT | 8am PT Zoom below 133
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 08/05/2026OBS Immunology Seminar| Young Investigator Highlight 'Comprehensive genetic manipulation of human immune cells' Theodore L. Roth, MD PhD Asst. Prof. of Pathology Stanford 🎙️@danmcmanus123.bsky.social Thurs, May 14th, 2026 1pm ET | 12pm CT | 10am PT Zoom below 141
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 01/03/2026us02web.zoom.us/meeting/regi... 011
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 24/02/2026NOW ON YOUTUBE youtu.be/5OHRntr_McU 'Understanding CD8+ T cell Tolerance' Ian Parish, PhD Assoc. Professor & Group Leader, Peter MacCallum Cancer Centre lnkd.in/gkXdZbRy @IanParish_AU 🎙️@dmcmanus123 Thursday, Feb 19th, 2026 5pm ET | 4pm CT | 2pm PT Zoom👇️ lnkd.in/gQime6rC 032
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 23/02/2026Upcoming OBS Immunology seminar: Disentangling Tissue-resident Memory and Exhausted CD8 T cells 🎙️Speaker Highlight 🔬Simone L. Park, PhD, UPenn, Wherry Lab 📢 Paper in Nature Immunology (2026) Feb 26th, 2026 | ⏰5pm ET/ 4pm CT/ 2pm PT Zoom: us02web.zoom.us/meeting/regi... 011
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 23/02/2026Upcoming OBS Immunology seminar: Disentangling Tissue-resident Memory and Exhausted CD8 T cells Speaker Highlight 🔬Thomas N. Burn, PhD, Uni of Melbourne, Mackay Lab 📢 Paper in Nature Immunology (2026) Feb 26th, 2026 | ⏰5pm ET/ 4pm CT/ 2pm PT Zoom: us02web.zoom.us/meeting/regi... 011
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 20/02/2026OBS Immunology Seminar Disentangling Tissue-resident Memory and Exhausted CD8 T cells 📢Two papers published back-to-back in @natimmunol.nature.com (2026)! Feb 26, 2026 | ⏰5pm ET/ 4pm CT/ 2pm PT 🔬 Simone Park 🧪 Tom Burn 🎙️ Dan McManus @danmcmanus123.bsky.social Zoom link🧵👇️ 132
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 13/02/2026OBS Immunology | Young Investigator Seminar 'Understanding CD8+ T cell Tolerance' Ian Parish, PhD Assoc. Professor & Group Leader, Peter MacCallum Cancer Centre tinyurl.com/Parish-Lab @ianparish.bsky.social 🎙️@danmcmanus123.bsky.social Thursday, Feb 19th, 2026 5pm ET | 4pm CT | 2pm PT Zoom👇️ 232
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 11/02/2026TOMORROW AT 11AM EST! Young Investigator Seminar 'Dissecting T cell Differentiation in Chronic Settings' Mohamed Abdel Hakeem, PhD, MSc, BPharm Asst. Professor, Emory University tinyurl.com/Hakeem-Lab 📅 Thursday, Feb 12th, 2026 ⏰ 11am ET | 10am CT | 8am PT us02web.zoom.us/meeting/regi... 011
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 05/02/2026OBS Immunology | Young Investigator Seminar 'Dissecting T cell Differentiation in Chronic Settings' Mohamed Abdel Hakeem, PhD, MSc, BPharm Asst. Professor, Emory University tinyurl.com/Hakeem-Lab 📅 Thursday, Feb 12th, 2026 ⏰ 11am ET | 10am CT | 8am PT us02web.zoom.us/meeting/regi... 032
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 23/01/2026OBS Immunology | Young Investigator Seminar 'Tissue Context as a Regulator of CD8⁺ T Cell Function' William H. Hudson, PhD Assistant Professor, Baylor College of Medicine hudsonlaboratory.com 📅 Thursday, Jan 29, 2026 ⏰ 11am ET | 10am CT | 8am PT zoom: us02web.zoom.us/meeting/regi... 011
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 16/01/2026Immunology Lymph nodes as hubs for productive CD8 T cell immunity 📢Two papers from Axel Kallies' lab in @natimmunol.nature.com (2025)! Jan 22, 2026 | ⏰5pm ET/ 4pm CT/ 2pm PT 🔬Sharanya Wijesinghe 🧪Carlson Tsui 🎙️@dmcmanus123 Zoom: us02web.zoom.us/meeting/regi... 041
Reposted by Daniel McManus, PhDOpen Box Science @openboxscience.bsky.social · 06/12/2025Don't miss the Nature Reviews Immunology webinar on new T cell nomenclature guidelines! Monday, Dec 8, 2025 09:00 PST | 12:00 EST | 17:00 GMT | 18:00 CET Register here: t.co/pe6GYY4AsSt.cohttps://z.umn.edu/Tcellnomenclature 063
Reposted by Daniel McManus, PhDProf. Shane Crotty @profshanecrotty.bsky.social · 12/11/2025In our latest study in @cp-immunity.bsky.social, we followed dynamics of vaccine-specific Tfh cells for 60+ weeks after immunization. authors.elsevier.com/a/1m3mV3qNrU... We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail. 2268
Reposted by Daniel McManus, PhDNature [Unofficial] @nature.com.web.brid.gy · 06/10/2025nature.comMedicine Nobel goes to scientists who revealed secrets of immune system ‘regulation’Nature, Published online: 06 October 2025; doi:10.1038/d41586-025-03193-3 Mary Brunkow, Fred Ramsdell and Shimon Sakaguchi discovered cells that protect the body from autoimmune diseases. 011
Reposted by Daniel McManus, PhDnatimmunol.nature.com @natimmunol.nature.com · 06/10/2025Congratulations to Fred Ramsdell, Mary Brunkow and Shimon Sakaguchi - the 2025 Winners of the Nobel Prize in Physiology or Medicine. #Treg_cells #Foxp3 #Immune_regulation 0206
Reposted by Daniel McManus, PhDWaggoner Lab @labwaggoner.bsky.social · 22/09/2025CD7 regulates the persistence of terminally exhausted CD8+ T cells during chronic infection @cp-cellreports.bsky.social www.cell.com/cell-reports... @bcmhouston.bsky.social 162
Daniel McManus, PhD @danmcmanus123.bsky.social · 22/09/2025Great new work from Will Hudson’s lab at @bcmhouston.bsky.social on the role of CD7 during CD8 T cell exhaustion! tinyurl.com/3c5mdyzr hudsonlaboratory.com @cp-cellreports.bsky.socialtinyurl.comCD7 regulates the persistence of terminally exhausted CD8+ T cells during chronic infectionHyslop et al. identify CD7 as a tissue-specific receptor essential for maintaining terminally exhausted CD8+ T cells during chronic infection. CD7 is highly expressed on a CD101+Tim3low liver-resident... 000
Reposted by Daniel McManus, PhDAdrian Liston @labliston.bsky.social · 19/09/2025For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper? You got it: currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...currentprotocols.onlinelibrary.wiley.com 1015869
Reposted by Daniel McManus, PhDDaniel L. Barber, PhD @danbarberphd.bsky.social · 17/09/2025We are looking to hire post-docs to work on T cell responses to M. tuberculosis infection using murine and NHP models. If you know T cells and wanna work on the top global infectious killer check us out. BSL3 experience not required. The NIH intramural program in Bethesda MD is an amazing place! 02015
Reposted by Daniel McManus, PhDYannick Alexandre @yannick-alexandre.bsky.social · 05/06/2025I’m pleased to share my latest work, published in Science Advances. In this study, we investigated how fibroblastic reticular cells (FRCs) that populate the white pulp of the spleen regulate immune responses to viral infections. #immunology #immunosky www.science.org/doi/10.1126/...science.orgSplenic fibroblastic reticular cells orchestrate dendritic cell maturation and facilitate CD8+ T cell priming and protective memoryFibroblastic reticular cells of the spleen coordinate inflammation for optimal antiviral T cell responses and protective memory. 163
Reposted by Daniel McManus, PhDProf. Shane Crotty @profshanecrotty.bsky.social · 07/04/2025One of our obsessions in the Crotty lab is to better understand B-cell immunology in the context of vaccines. Rare B cells can have antibody specificities particularly important for protection against some diseases. In our most recent paper… @cp-immunity.bsky.social www.cell.com/immunity/ful...cell.comDiverse priming outcomes under conditions of very rare precursor B cellsB cells that give rise to neutralizing antibody responses can be extremely rare in the naive B cell repertoire. Madden et al. show that delivery strategy and adjuvant selection affect the recruitment ... 16418
Reposted by Daniel McManus, PhDImmunity @cp-immunity.bsky.social · 14/03/2025dlvr.itCollaboration between interleukin-7 and -15 enables adaptation of tissue-resident and circulating memory CD8+ T cells to cytokine deficiencyCirculating memory CD8+ T cells are thought to be highly dependent on interleukin (IL)-7 signaling. Using inducible Il7ra deletion in circulating and tissue-resident memory CD8+ T cell subsets, Jarjour et al. reveal resilience to loss of IL-7 signaling… 042
Daniel McManus, PhD @danmcmanus123.bsky.social · 14/03/2025Fantastic talk and beautiful story from Maria Cardenas and @haydnkissick.bsky.social lab! Hosted by @openboxscience.bsky.social youtu.be/rC69rAz6OeEyoutu.be[Maria Cardenas] Differentiation fate of a stem-like CD4 T cell controls immunity to cancerYouTube video by Open Box Science 010
Reposted by Daniel McManus, PhDJoanna Groom @groomlab.bsky.social · 11/03/2025Very excited our work out in @jem.org “Transient inhibition of type I interferon enhances CD8+ T cell stemness and vaccine protection” @wehi-research.bsky.social led by talented (and determined) PhD student @benjbroom.bsky.social Thread below. 25622
Daniel McManus, PhD @danmcmanus123.bsky.social · 03/03/2025chng.it/YPmBFkS8xJchng.itThis campaign needs you nowPetition to Reverse the NIH Indirect Cost Cap (NOT-OD-25-068) 000
Reposted by Daniel McManus, PhDE John Wherry @ejohnwherry.bsky.social · 23/02/2025Excellent Info Graphic. Share everywhere! 01812
Reposted by Daniel McManus, PhDMiguel Reina-Campos @reinacampos.bsky.social · 19/02/2025Using PAXgene as an alternative tissue preservative for spatial transcriptomics with #Xenium @10xgenomics.bsky.social - An insane amount of work and a very methodical investigationbiorxiv.org 073
Reposted by Daniel McManus, PhDNik Joshi @joshilabyale.bsky.social · 18/02/2025My wife and I were postbac IRTAs 25 years ago. Neither would have pursued a career in research if not for this program We have PhD students in Yale Immunobiology now who also came from this NIH program including (one amazing student in my lab) This is a valuable program and has been for decades. 0143
Reposted by Daniel McManus, PhDColin Raposo @colinraposo.bsky.social · 16/02/2025I’m excited to share our lab’s new preprint! We find that some exhausted T cell clones maintain the capacity form functional memory cells: www.biorxiv.org/content/10.1... 1/ 🧵biorxiv.org 133
Reposted by Daniel McManus, PhDDaniel Utzschneider @danielutzschneider.bsky.social · 31/01/2025Excited for our lab's FIRST paper #ScienceImmunology! 🎉 Led by @categraca.bsky.social & Amania Sheikh, with amazing collabs from Axel Kallies, Dane Newman, & @rickyjohnstone.bsky.social Huge thanks to reviewers & @claireolingy.bsky.social 🙌 #Tpex #onthescoreboard www.science.org/doi/full/10....science.orgStem-like memory and precursors of exhausted T cells share a common progenitor defined by ID3 expressionID3 defines stem-like T cells with high developmental potential that sustain T cell responses to chronic infection and cancer. 2357
Reposted by Daniel McManus, PhDWaggoner Lab @labwaggoner.bsky.social · 24/01/2025ImmunOctoberfest reloaded @natimmunol.bsky.social @annisch.bsky.social www.nature.com/articles/s41... 021
Reposted by Daniel McManus, PhDVineet Joag @vjoag.bsky.social · 22/01/2025Super excited to announce that the Joag Lab will officially open at @UW, @SCRI in March 2025! We will study the interplay between memory T cells and stromal, humoral, and innate immunity in mucosal tissues. researcherprofiles.seattlechildrens.org/Vineet.Joag. Hiring a technician and a postdoc! 2159
Daniel McManus, PhD @danmcmanus123.bsky.social · 15/01/2025Special thanks to my PhD mentor Rafi, my co-first author, Rajesh Valanparambil, and all the rest of our co-authors. Image above shows CD8 T cells asking a crystal ball to predict infection outcome. Credit: ChatGPT4 Also see our companion paper from Dietmar Zehn's lab: tinyurl.com/yck3mduptinyurl.comPrecursors of exhausted T cells are preemptively formed in acute infection - NatureNature - Precursors of exhausted T cells are preemptively formed in acute infection 032
Daniel McManus, PhD @danmcmanus123.bsky.social · 15/01/2025Excited to share my PhD work from Rafi Ahmed’s lab, now published in @nature.com. We show how CD8 T cells overcome the inability to predict how long a viral infection will last by making adaptable precursor cells a priori—an elegant strategy for a non-prescient immune system! tinyurl.com/53nrpsa6 154
Reposted by Daniel McManus, PhDPayal Yokota @payalyokota.bsky.social · 11/01/2025Two beautiful stories out this month in Nature (Jan8, 2025) about the enigmatic decision making of stem cell like CD8+ T cells from Rafi and Dietmar's labs. Have a look! www.nature.com/articles/s41... www.nature.com/articles/s41... 1136
Reposted by Daniel McManus, PhDDaniel Utzschneider @danielutzschneider.bsky.social · 09/01/2025Two studies published in @nature.com today showing that Tpex-like cells develop independent from acute or chronic infection #Tpex www.nature.com/articles/s41...nature.comPrecursors of exhausted T cells are preemptively formed in acute infection - NatureNature - Precursors of exhausted T cells are preemptively formed in acute infection 184
