The slow growth displayed by Mycobacterium tuberculosis has been historically associated with pathogen’s persistence and drug tolerance. Unexpectedly, here the authors identify a rare subpopulation of...

In cells, ESCRT-III is unique in mediating fission of membrane necks from inside, a process called reverse-topology fission. Yet, in vitro, the complex primarily assembles outside membrane necks and m...

Endomembrane damage of intracellular vesicles triggers signals that activate membrane repair in mammalian cells to restore homeostasis. However, the signals that drive diverse membrane repair recruitm...

Sterol-rich microdomain accumulation at the MCV is crucial for Mm-induced damage and infection in D. discoideum and BV-2 cells.

CRISPRI-mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI-med...

The emergence of cell compartmentalization depends on membrane fission to create the endomembrane compartments. In eukaryotes, membrane fission is commonly executed by ESCRT-III, a protein complex con...

Tuberculosis remains the deadliest infectious disease caused by a single pathogen, highlighting the urgent need for novel therapies. A deeper under...

Various vibrios can colonise a large vacuole called VCV (Vibrio-containing-vacuole) within Paramoeba atlantica. The VCVs acidify and the vibrios multiply and remain alive inside the VCVs for hours. T...

The metabolism of ergosterol shapes this lipid to allow the formation of fluid membrane domains in yeast cells.

Various vibrios can colonise a large vacuole called VCV (Vibrio-containing-vacuole) within Paramoeba atlantica. The VCVs acidify and the vibrios multiply and remain alive inside the VCVs for hours. T....